anti-lim1 Search Results


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  • 85
    Millipore anti lim1
    The SHH signaling pathway plays a pivotal and orchestral role in the constitutive activation of oncogenic pathways in human CRCC . ( A ) Western blots analysis of human CRCC 786-0 cell lysates treated for 2 days in control (Ctl) or with cyclopamine (Cy) at 20 μM and incubated with the antibodies against non-phosphorylated GSK-3 (GSK-3), phospho-GSK-3 (P-GSK3), non-phosphorylated Akt (Akt), phospho-Akt (P-Akt), non-phosphorylated NF-κB (NF-κB), phospho-NF-κB (P-NF-κB), non-phosphorylated Erk1/2 (Erk1/2), phospho-Erk1/2 (P-Erk1/2) and corresponding β-actin. The gels shown are representative for at least 3 independent experiments. ( B ) Western blots analysis of human CRCC 786-0 cells lysates treated for 2 days in control (Ctl) or with cyclopamine (Cy) at 20 μM and incubated with the antibodies against Gli1, cyclin D1, Pax2, <t>Lim1,</t> VEGF, TGF-β1 and corresponding β-actin. The gels shown are representative for at least 3 independent experiments. ( C ) Western blot analysis in 9 human tumors (T1 ...) and normal corresponding tissues (N1 ...) lysates incubated with antibodies against Gli1, cyclin D1, Pax2, Lim1, and corresponding β-actin. The gels shown are representative for at least 3 independent experiments.
    Anti Lim1, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Developmental Studies Hybridoma Bank lim1
    Cell proliferation and early development in the G9a Dkk3 CKO retina. A–L , Immunofluorescence staining of the retinas at E14.5. Retinal sections of control ( A , C , E , G , I , K ) and G9a Dkk3 CKO ( B , D , F , H , J , L ) were stained with markers for Otx2 + and Crx + photoreceptor precursors ( A–D ), Thrβ2 + cone photoreceptor precursor cells ( E , F ), Prox1 + and <t>Lim1</t> + horizontal cells ( G , H ), Pax6 + amacrine cells ( I , J ), Brn3b + ganglion cells ( K , L ), and Chx10 + retinal progenitor cells ( E , F ). Blue staining is a nuclear dye. For early generated cell types, cell fate determination was normal in the G9a Dkk3 CKO retina. Scale bars, 50 μm.
    Lim1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 88/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lim1/product/Developmental Studies Hybridoma Bank
    Average 88 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    lim1 - by Bioz Stars, 2020-08
    88/100 stars
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    91
    Abcam rabbit anti lim1 antibody
    Cell proliferation and early development in the G9a Dkk3 CKO retina. A–L , Immunofluorescence staining of the retinas at E14.5. Retinal sections of control ( A , C , E , G , I , K ) and G9a Dkk3 CKO ( B , D , F , H , J , L ) were stained with markers for Otx2 + and Crx + photoreceptor precursors ( A–D ), Thrβ2 + cone photoreceptor precursor cells ( E , F ), Prox1 + and <t>Lim1</t> + horizontal cells ( G , H ), Pax6 + amacrine cells ( I , J ), Brn3b + ganglion cells ( K , L ), and Chx10 + retinal progenitor cells ( E , F ). Blue staining is a nuclear dye. For early generated cell types, cell fate determination was normal in the G9a Dkk3 CKO retina. Scale bars, 50 μm.
    Rabbit Anti Lim1 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti lim1 antibody/product/Abcam
    Average 91 stars, based on 15 article reviews
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    rabbit anti lim1 antibody - by Bioz Stars, 2020-08
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    88
    Developmental Studies Hybridoma Bank anti lim1 2
    P450c17 expression in the developing spinal cord in the mouse . (A) Immunostaining of P450c17 in E13.5 sagittal sections of the spinal cord. (B) Co-expression of P450c17 (green) with Isl-1, Isl2, <t>Lim1/2,</t> Lim3, and HB9 (red) in an E13.5 transverse section of whole embryo (thoracic level of the spinal cord). For each marker, an overlay with P450c17 is shown, as well as a high magnification view. P450c17 being a microsomal protein, its staining is found both in cell bodies and in fibers, whereas Isl-1, Isl2, Lim1/2, Lim3, and HB9 are nuclear proteins. Asterisks indicate cells dually labeled. (A–C) Bars equal 100 μm. (D) Schematic representation of the expression domains of P450c17 in the different domains of motor neuron subtypes markers.
    Anti Lim1 2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 88/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 63 article reviews
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    anti lim1 2 - by Bioz Stars, 2020-08
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    85
    Developmental Studies Hybridoma Bank mouse anti lim1
    Lgr5 is expressed in the early female reproductive tract during embryogenesis. a RNA ISH for Lgr5 in coelomic epithelium at E12.0. b The Lgr5-2A-EGFP mouse model employed to evaluate endogenous Lgr5 expression. c Co-IF for Lgr5-EGFP and <t>Lim1</t> in coelomic epithelium at E12.0. d Confocal z-stack image of a whole-mount E12.5 Müllerian duct (highlighted by the red dashed line). Yellow box indicates the region magnified in e . e Endogenous EGFP fluorescence in E12.5 Lgr5-2A-EGFP mouse at Md. f Immunostaining for Lgr5-EGFP and E-cadherin in P0 uterus. Dashed red lines indicate Md, and dashed black or white lines indicate Wd, respectively. Md, Müllerian duct; Wd, Wolffian duct; Scale bars, 50 μm. All images are representative of three independent mice.
    Mouse Anti Lim1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 85/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Millipore rabbit anti lim1
    Loss of HCs in Oc1 -null retinas occurs at early developmental stages. A–H , Immunolabeling for <t>Lim1</t> and Prox1 at E14.5. Lim1 and Prox1 are expressed in newly generated HCs located at the boundary between the GCL and the NBL ( A , C , E , G ). Prox1
    Rabbit Anti Lim1, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti lim1/product/Millipore
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    lim1  (Abcam)
    90
    Abcam lim1
    Loss of HCs in Oc1 -null retinas occurs at early developmental stages. A–H , Immunolabeling for <t>Lim1</t> and Prox1 at E14.5. Lim1 and Prox1 are expressed in newly generated HCs located at the boundary between the GCL and the NBL ( A , C , E , G ). Prox1
    Lim1, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lim1/product/Abcam
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    91
    Developmental Studies Hybridoma Bank mouse anti lim1 lhx1
    Loss of HCs in Oc1 -null retinas occurs at early developmental stages. A–H , Immunolabeling for <t>Lim1</t> and Prox1 at E14.5. Lim1 and Prox1 are expressed in newly generated HCs located at the boundary between the GCL and the NBL ( A , C , E , G ). Prox1
    Mouse Anti Lim1 Lhx1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Developmental Studies Hybridoma Bank mouse igg1 anti lim1
    Loss of HCs in Oc1 -null retinas occurs at early developmental stages. A–H , Immunolabeling for <t>Lim1</t> and Prox1 at E14.5. Lim1 and Prox1 are expressed in newly generated HCs located at the boundary between the GCL and the NBL ( A , C , E , G ). Prox1
    Mouse Igg1 Anti Lim1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Developmental Studies Hybridoma Bank anti lim1 2 4f2
    Loss of HCs in Oc1 -null retinas occurs at early developmental stages. A–H , Immunolabeling for <t>Lim1</t> and Prox1 at E14.5. Lim1 and Prox1 are expressed in newly generated HCs located at the boundary between the GCL and the NBL ( A , C , E , G ). Prox1
    Anti Lim1 2 4f2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Developmental Studies Hybridoma Bank anti lim1 2 4f2 mouse monoclonal
    Loss of HCs in Oc1 -null retinas occurs at early developmental stages. A–H , Immunolabeling for <t>Lim1</t> and Prox1 at E14.5. Lim1 and Prox1 are expressed in newly generated HCs located at the boundary between the GCL and the NBL ( A , C , E , G ). Prox1
    Anti Lim1 2 4f2 Mouse Monoclonal, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The SHH signaling pathway plays a pivotal and orchestral role in the constitutive activation of oncogenic pathways in human CRCC . ( A ) Western blots analysis of human CRCC 786-0 cell lysates treated for 2 days in control (Ctl) or with cyclopamine (Cy) at 20 μM and incubated with the antibodies against non-phosphorylated GSK-3 (GSK-3), phospho-GSK-3 (P-GSK3), non-phosphorylated Akt (Akt), phospho-Akt (P-Akt), non-phosphorylated NF-κB (NF-κB), phospho-NF-κB (P-NF-κB), non-phosphorylated Erk1/2 (Erk1/2), phospho-Erk1/2 (P-Erk1/2) and corresponding β-actin. The gels shown are representative for at least 3 independent experiments. ( B ) Western blots analysis of human CRCC 786-0 cells lysates treated for 2 days in control (Ctl) or with cyclopamine (Cy) at 20 μM and incubated with the antibodies against Gli1, cyclin D1, Pax2, Lim1, VEGF, TGF-β1 and corresponding β-actin. The gels shown are representative for at least 3 independent experiments. ( C ) Western blot analysis in 9 human tumors (T1 ...) and normal corresponding tissues (N1 ...) lysates incubated with antibodies against Gli1, cyclin D1, Pax2, Lim1, and corresponding β-actin. The gels shown are representative for at least 3 independent experiments.

    Journal: Molecular Cancer

    Article Title: The sonic hedgehog signaling pathway is reactivated in human renal cell carcinoma and plays orchestral role in tumor growth

    doi: 10.1186/1476-4598-8-123

    Figure Lengend Snippet: The SHH signaling pathway plays a pivotal and orchestral role in the constitutive activation of oncogenic pathways in human CRCC . ( A ) Western blots analysis of human CRCC 786-0 cell lysates treated for 2 days in control (Ctl) or with cyclopamine (Cy) at 20 μM and incubated with the antibodies against non-phosphorylated GSK-3 (GSK-3), phospho-GSK-3 (P-GSK3), non-phosphorylated Akt (Akt), phospho-Akt (P-Akt), non-phosphorylated NF-κB (NF-κB), phospho-NF-κB (P-NF-κB), non-phosphorylated Erk1/2 (Erk1/2), phospho-Erk1/2 (P-Erk1/2) and corresponding β-actin. The gels shown are representative for at least 3 independent experiments. ( B ) Western blots analysis of human CRCC 786-0 cells lysates treated for 2 days in control (Ctl) or with cyclopamine (Cy) at 20 μM and incubated with the antibodies against Gli1, cyclin D1, Pax2, Lim1, VEGF, TGF-β1 and corresponding β-actin. The gels shown are representative for at least 3 independent experiments. ( C ) Western blot analysis in 9 human tumors (T1 ...) and normal corresponding tissues (N1 ...) lysates incubated with antibodies against Gli1, cyclin D1, Pax2, Lim1, and corresponding β-actin. The gels shown are representative for at least 3 independent experiments.

    Article Snippet: Membranes were incubated overnight at 4°C with the appropriate dilution of the following primary antibodies: anti-Akt antibody (1:250; Millipore), anti-phospho-Akt antibody (1:150; Ozyme, Cell signaling local distributor, Saint-Quentin-en-Yvelines, France), anti-GSK3α/β antibody (1:1000; Millipore 05-903), anti-phospho-GSK3α/β (Ser21/9) antibody (1:250; Ozyme), anti-NF-κB (1:2000; Millipore AB1604), anti-phospho-NF-κB (S468) (1:250; Ozyme); anti-Erk1/(1:1000; Ozyme), anti-phospho-Erk1/2 (1:1000; Millipore 05-481), anti-SHH (1:500; Ozyme), anti-cyclinD1 (1:750; Ozyme), anti-Gli1 (1:2000; Millipore AB3444), anti-Pax2 (1:1000; Ozyme), anti-Lim1 (1:3000; Millipore AB3200), anti-VEGF (1:250; Millipore MAB3734) and anti-TGFβ1 (1:200; Ozyme).

    Techniques: Activation Assay, Western Blot, CTL Assay, Incubation

    Abnormal gene expression in the patches. A–R , In situ hybridization of E12.5 and E16.5 Otx1 cre/+ ; Otx2 +/− and conditional mutants with Gli1 ( A , F ), Gli2 ( B , G ), Gli3 ( C , H ), Ptch1 ( D , I ), Smo ( E , J ), Lim1 ( K , O ), Pax3 ( L , P ), Pax7 ( M , Q ), and Gbx2 ( N , R ) probes. Note that Gbx2 is lost in the patches in which the expression of Lim1 , Pax3 , and Pax7 is activated. For control embryos, the sections at E12.5 from A–D and from E–N are two different groups of adjacent sections; similarly, also for the conditional mutant, the sections at E12.5 from A–D and from E–N are two groups of adjacent sections belonging to two different embryos. Th, Thalamus.

    Journal: The Journal of Neuroscience

    Article Title: Otx2 Controls Identity and Fate of Glutamatergic Progenitors of the Thalamus by Repressing GABAergic Differentiation

    doi: 10.1523/JNEUROSCI.1097-06.2006

    Figure Lengend Snippet: Abnormal gene expression in the patches. A–R , In situ hybridization of E12.5 and E16.5 Otx1 cre/+ ; Otx2 +/− and conditional mutants with Gli1 ( A , F ), Gli2 ( B , G ), Gli3 ( C , H ), Ptch1 ( D , I ), Smo ( E , J ), Lim1 ( K , O ), Pax3 ( L , P ), Pax7 ( M , Q ), and Gbx2 ( N , R ) probes. Note that Gbx2 is lost in the patches in which the expression of Lim1 , Pax3 , and Pax7 is activated. For control embryos, the sections at E12.5 from A–D and from E–N are two different groups of adjacent sections; similarly, also for the conditional mutant, the sections at E12.5 from A–D and from E–N are two groups of adjacent sections belonging to two different embryos. Th, Thalamus.

    Article Snippet: The rabbit antibodies were directed against Otx2 (1:5000; G. Corte, National Institute for Cancer Research, Genova, Italy), Pax3 (1:200; Zymed, San Francisco, CA), GABA (1:500; Sigma, St. Louis, MO), Lim1 (1:200; Chemicon, Temecula, CA), VGLUT2 (1:300; SySy, Goettingen, Germany), and Ph-H3 (the phosphorylated form of histone H3) (1:200; Upstate, Charlottesville, VA).

    Techniques: Expressing, In Situ Hybridization, Mutagenesis

    Cell-type identity in the patches. A–H , Combinatorial immunodetection with Otx2 ( A , C , E , H ), Pax3/7 ( A , C , D , F ) and Lim1 ( B , D , E–G ) antibodies. The sections at E10.5 and E16.5 are counterstained with Hoechst. The red color for Pax3/7 ( D ) is a pseudocolor. V, Ventricle.

    Journal: The Journal of Neuroscience

    Article Title: Otx2 Controls Identity and Fate of Glutamatergic Progenitors of the Thalamus by Repressing GABAergic Differentiation

    doi: 10.1523/JNEUROSCI.1097-06.2006

    Figure Lengend Snippet: Cell-type identity in the patches. A–H , Combinatorial immunodetection with Otx2 ( A , C , E , H ), Pax3/7 ( A , C , D , F ) and Lim1 ( B , D , E–G ) antibodies. The sections at E10.5 and E16.5 are counterstained with Hoechst. The red color for Pax3/7 ( D ) is a pseudocolor. V, Ventricle.

    Article Snippet: The rabbit antibodies were directed against Otx2 (1:5000; G. Corte, National Institute for Cancer Research, Genova, Italy), Pax3 (1:200; Zymed, San Francisco, CA), GABA (1:500; Sigma, St. Louis, MO), Lim1 (1:200; Chemicon, Temecula, CA), VGLUT2 (1:300; SySy, Goettingen, Germany), and Ph-H3 (the phosphorylated form of histone H3) (1:200; Upstate, Charlottesville, VA).

    Techniques: Immunodetection

    Proliferating activity and cell fate analysis. A–T , Immunodetection of Pax3/7 and BrdU ( A–D , I–L ), BrdU alone ( M–P ), and Ph-H3 ( E–H , Q–T ) in embryos exposed to a short pulse of BrdU at E10.5, E12.5, E13.5, and E16.5. Note that, at E12.5 and particularly at E13.5, the density of BrdU + and Ph-H3 + cells within the patches ( J , K , N , O , R , S ) is remarkably increased when compared with the unaffected neuroepithelium of conditional mutants (yellow arrow in J , K , N , O , R , S ) or Otx1 cre/+ ; Otx2 +/− control embryos ( B , C , F , G ). The red arrows in D point to the sporadic BrdU + cells detected at E16.5 in control embryos, and the white arrows in Q–T point to the Ph-H3 + cells in the patches. U–W , BrdU long-pulse experiments show that BrdU + cells labeled between E13.3 and E13.6 accumulate at E16.5 in the patches without mixing with those labeled along the unaffected neuroepithelium ( U ), and most of them are Lim1 + ( V ). Note that BrdU + -Lim1 − cells (arrowheads in the magnifications) may correspond to additional cell type(s) of the patches ( V , W ).

    Journal: The Journal of Neuroscience

    Article Title: Otx2 Controls Identity and Fate of Glutamatergic Progenitors of the Thalamus by Repressing GABAergic Differentiation

    doi: 10.1523/JNEUROSCI.1097-06.2006

    Figure Lengend Snippet: Proliferating activity and cell fate analysis. A–T , Immunodetection of Pax3/7 and BrdU ( A–D , I–L ), BrdU alone ( M–P ), and Ph-H3 ( E–H , Q–T ) in embryos exposed to a short pulse of BrdU at E10.5, E12.5, E13.5, and E16.5. Note that, at E12.5 and particularly at E13.5, the density of BrdU + and Ph-H3 + cells within the patches ( J , K , N , O , R , S ) is remarkably increased when compared with the unaffected neuroepithelium of conditional mutants (yellow arrow in J , K , N , O , R , S ) or Otx1 cre/+ ; Otx2 +/− control embryos ( B , C , F , G ). The red arrows in D point to the sporadic BrdU + cells detected at E16.5 in control embryos, and the white arrows in Q–T point to the Ph-H3 + cells in the patches. U–W , BrdU long-pulse experiments show that BrdU + cells labeled between E13.3 and E13.6 accumulate at E16.5 in the patches without mixing with those labeled along the unaffected neuroepithelium ( U ), and most of them are Lim1 + ( V ). Note that BrdU + -Lim1 − cells (arrowheads in the magnifications) may correspond to additional cell type(s) of the patches ( V , W ).

    Article Snippet: The rabbit antibodies were directed against Otx2 (1:5000; G. Corte, National Institute for Cancer Research, Genova, Italy), Pax3 (1:200; Zymed, San Francisco, CA), GABA (1:500; Sigma, St. Louis, MO), Lim1 (1:200; Chemicon, Temecula, CA), VGLUT2 (1:300; SySy, Goettingen, Germany), and Ph-H3 (the phosphorylated form of histone H3) (1:200; Upstate, Charlottesville, VA).

    Techniques: Activity Assay, Immunodetection, Labeling

    GABAergic fate switch of glutamatergic progenitors. A–C , In situ hybridization ( A , B ) and immunohistochemistry ( C ) of E16.5 Otx1 cre/+ ; Otx2 +/− and Otx1 cre/+ ; Otx2 flox/− embryos with VGLUT2 ( A ), Gad1 ( B ) probes, and GABA ( C ) antibody show that, in the patches, the lack of VGLUT2 expression correlates with the induction of GABAergic markers. D–G , Combinatorial immunodetection with GABA ( D , E ), VGLUT2 ( D , F , G ), and BrdU ( E , F ) antibodies in E16.5 conditional mutants labeled at E13.3 with BrdU shows that many of the BrdU + neurons are GABA + , although none of them are VGLUT2 + . H–M , Combinatorial immunodetection with GABA ( H–K ), Lim1 ( H , I , L , M ), and Pax3/7 ( J , K ) antibodies shows that GABA + neurons are Lim1 + . I , K , and M correspond to the magnification of the area demarcated in H , J , and L . The green color for VGLUT2 ( D ) is a pseudocolor.

    Journal: The Journal of Neuroscience

    Article Title: Otx2 Controls Identity and Fate of Glutamatergic Progenitors of the Thalamus by Repressing GABAergic Differentiation

    doi: 10.1523/JNEUROSCI.1097-06.2006

    Figure Lengend Snippet: GABAergic fate switch of glutamatergic progenitors. A–C , In situ hybridization ( A , B ) and immunohistochemistry ( C ) of E16.5 Otx1 cre/+ ; Otx2 +/− and Otx1 cre/+ ; Otx2 flox/− embryos with VGLUT2 ( A ), Gad1 ( B ) probes, and GABA ( C ) antibody show that, in the patches, the lack of VGLUT2 expression correlates with the induction of GABAergic markers. D–G , Combinatorial immunodetection with GABA ( D , E ), VGLUT2 ( D , F , G ), and BrdU ( E , F ) antibodies in E16.5 conditional mutants labeled at E13.3 with BrdU shows that many of the BrdU + neurons are GABA + , although none of them are VGLUT2 + . H–M , Combinatorial immunodetection with GABA ( H–K ), Lim1 ( H , I , L , M ), and Pax3/7 ( J , K ) antibodies shows that GABA + neurons are Lim1 + . I , K , and M correspond to the magnification of the area demarcated in H , J , and L . The green color for VGLUT2 ( D ) is a pseudocolor.

    Article Snippet: The rabbit antibodies were directed against Otx2 (1:5000; G. Corte, National Institute for Cancer Research, Genova, Italy), Pax3 (1:200; Zymed, San Francisco, CA), GABA (1:500; Sigma, St. Louis, MO), Lim1 (1:200; Chemicon, Temecula, CA), VGLUT2 (1:300; SySy, Goettingen, Germany), and Ph-H3 (the phosphorylated form of histone H3) (1:200; Upstate, Charlottesville, VA).

    Techniques: In Situ Hybridization, Immunohistochemistry, Expressing, Immunodetection, Labeling

    Cell proliferation and early development in the G9a Dkk3 CKO retina. A–L , Immunofluorescence staining of the retinas at E14.5. Retinal sections of control ( A , C , E , G , I , K ) and G9a Dkk3 CKO ( B , D , F , H , J , L ) were stained with markers for Otx2 + and Crx + photoreceptor precursors ( A–D ), Thrβ2 + cone photoreceptor precursor cells ( E , F ), Prox1 + and Lim1 + horizontal cells ( G , H ), Pax6 + amacrine cells ( I , J ), Brn3b + ganglion cells ( K , L ), and Chx10 + retinal progenitor cells ( E , F ). Blue staining is a nuclear dye. For early generated cell types, cell fate determination was normal in the G9a Dkk3 CKO retina. Scale bars, 50 μm.

    Journal: The Journal of Neuroscience

    Article Title: G9a Histone Methyltransferase Activity in Retinal Progenitors Is Essential for Proper Differentiation and Survival of Mouse Retinal Cells

    doi: 10.1523/JNEUROSCI.1869-12.2012

    Figure Lengend Snippet: Cell proliferation and early development in the G9a Dkk3 CKO retina. A–L , Immunofluorescence staining of the retinas at E14.5. Retinal sections of control ( A , C , E , G , I , K ) and G9a Dkk3 CKO ( B , D , F , H , J , L ) were stained with markers for Otx2 + and Crx + photoreceptor precursors ( A–D ), Thrβ2 + cone photoreceptor precursor cells ( E , F ), Prox1 + and Lim1 + horizontal cells ( G , H ), Pax6 + amacrine cells ( I , J ), Brn3b + ganglion cells ( K , L ), and Chx10 + retinal progenitor cells ( E , F ). Blue staining is a nuclear dye. For early generated cell types, cell fate determination was normal in the G9a Dkk3 CKO retina. Scale bars, 50 μm.

    Article Snippet: We used the following primary antibodies: mouse monoclonal antibodies specific to Brn3a (1:100; Millipore Bioscience Research Reagents), Calbindin (1:200; Zymed), Cyclin D3 (1:100; Cell Signaling Technology), G9a (1:100; Perseus Proteomics), Lim1 (1:100; Developmental Studies Hybridoma Bank), Pax6 (1:200; Developmental Studies Hybridoma Bank), proliferating cell nuclear antigen (PCNA) (1:500; Dako), Rhodopsin (RET-P1) (1:5000; Sigma), and S100β (1:1000; Sigma); rabbit polyclonal antibodies to active caspase 3 (1:200; Promega), Calbindin (1:1000; Calbiochem), Chx10 (1:100) , Crx (1:300) , glial fibrillary acidic protein (GFAP) (1:1000; Sigma), M-opsin (1:300; Millipore Bioscience Research Reagents), phospho-Histone H3 (pH3) (1:100; Millipore), Prox1 (1:2000; Millipore Bioscience Research Reagents), Recoverin (1:1000; Millipore Bioscience Research Reagents), and Sox9 (1:750; Millipore); goat polyclonal antibodies to Brn3b (1:100; Santa Cruz Biotechnology), Lhx2 (1:1000; Santa Cruz Biotechnology), Otx2 (1:500; R & D Systems), and S-opsin (1:500; Santa Cruz Biotechnology); and a guinea pig polyclonal antibody to Thrβ2 (1:100; Wako) ( ).

    Techniques: Immunofluorescence, Staining, Generated

    Mis-expression of pretectal neuron markers in the absence of Barhl2 . (a) Immunolabeling reveals that the confined expression of DBX1 (green) in thalamic progenitor cells is disrupted and that PAX7 (red) expression expands from the p1 domain to the presumptive p2 domain in the Barhl2 -null mice at E12.5. (b and c) A similar fate switch is seen by expression of LIM1 (green in b ) and Pax3 (c) , markers for prectectal neurons and LHX2 (red in b ) expression is down-regulated in the dorsal thalamic neurons of the Barhl2 -null mice at E12.5. Scale bars equal 200 µm.

    Journal: Molecular neurobiology

    Article Title: Barhl2 determines the early patterning of the diencephalon by regulating Shh

    doi: 10.1007/s12035-016-0001-5

    Figure Lengend Snippet: Mis-expression of pretectal neuron markers in the absence of Barhl2 . (a) Immunolabeling reveals that the confined expression of DBX1 (green) in thalamic progenitor cells is disrupted and that PAX7 (red) expression expands from the p1 domain to the presumptive p2 domain in the Barhl2 -null mice at E12.5. (b and c) A similar fate switch is seen by expression of LIM1 (green in b ) and Pax3 (c) , markers for prectectal neurons and LHX2 (red in b ) expression is down-regulated in the dorsal thalamic neurons of the Barhl2 -null mice at E12.5. Scale bars equal 200 µm.

    Article Snippet: The following antibodies were used for IHC: Anti-DBX1 (1:1,000, gift from Y. Nakagawa), Anti-DCC (1:500, Santa Cruz), Anti-GFP (1:1000, Abcam), Anti-L1 (1:200, Chemicon), Anti-LHX2 (1:200, Santa Cruz), Anti-LHX9 (1:200, Santa Cruz), Anti-LIM1 (1:500, DSHB), Anti-ASCL1 (1:200, R & D Systems), Anti-NKX2.2 (1:200, DSHB), Anti-PAX7 (1:200, DSHB), Anti-PROX1 (1:500, Covance), Anti-SERT (1:500, Immunostar), Anti-SHH (1:500, R & D Systems).

    Techniques: Expressing, Immunolabeling, Mouse Assay

    P450c17 expression in the developing spinal cord in the mouse . (A) Immunostaining of P450c17 in E13.5 sagittal sections of the spinal cord. (B) Co-expression of P450c17 (green) with Isl-1, Isl2, Lim1/2, Lim3, and HB9 (red) in an E13.5 transverse section of whole embryo (thoracic level of the spinal cord). For each marker, an overlay with P450c17 is shown, as well as a high magnification view. P450c17 being a microsomal protein, its staining is found both in cell bodies and in fibers, whereas Isl-1, Isl2, Lim1/2, Lim3, and HB9 are nuclear proteins. Asterisks indicate cells dually labeled. (A–C) Bars equal 100 μm. (D) Schematic representation of the expression domains of P450c17 in the different domains of motor neuron subtypes markers.

    Journal: Frontiers in Endocrinology

    Article Title: Dehydroepiandrosterone Biosynthesis, Role, and Mechanism of Action in the Developing Neural Tube

    doi: 10.3389/fendo.2012.00016

    Figure Lengend Snippet: P450c17 expression in the developing spinal cord in the mouse . (A) Immunostaining of P450c17 in E13.5 sagittal sections of the spinal cord. (B) Co-expression of P450c17 (green) with Isl-1, Isl2, Lim1/2, Lim3, and HB9 (red) in an E13.5 transverse section of whole embryo (thoracic level of the spinal cord). For each marker, an overlay with P450c17 is shown, as well as a high magnification view. P450c17 being a microsomal protein, its staining is found both in cell bodies and in fibers, whereas Isl-1, Isl2, Lim1/2, Lim3, and HB9 are nuclear proteins. Asterisks indicate cells dually labeled. (A–C) Bars equal 100 μm. (D) Schematic representation of the expression domains of P450c17 in the different domains of motor neuron subtypes markers.

    Article Snippet: Pools of motor neurons were identified by using anti-Isl-1/2 (40.2D6, DSHB) at 1:100, anti-Isl2 (gift from Dr. Pfaff) at 1:2,000, anti-Lim1/2 (4F2, DSHB) at 1:100, anti-Lim3 (gift from Dr. Pfaff) at 1:3,000, and anti-HB9 (gift from Dr. Pfaff) at 1:8,000.

    Techniques: Expressing, Immunostaining, Marker, Staining, Labeling

    Characterization of Wt1+ neurons in the developing spinal cord. (A) Schematic illustration and Wt1 immunolabelling analysis of a transverse section (12 μm) from E12.5 spinal cord showing the position of Wt1+ neurons (red) in the mantle zone of the developing spinal cord. Stippled line represents the border between the ventricular and mantle zones. Scale bar: 50 μm. (B) Plot showing the average cell number of Wt1+ neurons per 12 μm spinal cord section from different embryonic and postnatal stages. Wt1+ neurons are first found at E12.5 and decrease in cell number postnatally. Data expressed as mean ± SD. n = 12–20 embryos. (C) Determination of the birthdate of Wt1+ neurons by BrdU proliferation assay. Proliferating cells situated in the ventricular zone were labelled by BrdU incorporation at different embryonic stages (E9.5, E10.5, and E11.5). Additional immunolabelling of these cells for Wt1 and BrdU at E12.5 revealed that prospective Wt1 -expressing cells still proliferate at E9.5 and at E10.5 but not at E11.5. Scale bar: 10 μm. Insets show higher magnifications of respective areas. Scale bar: 5 μm. (D) Schematic illustration of an E12.5 spinal cord section with markers and their occurrence in different neuron populations. These markers were used to establish the origin of Wt1+ neurons as dI6 neurons (red). (E) Immunolabelling of Wt1+ neurons with markers present in dI6 and adjacent interneuron populations. The partly overlapping location of Wt1 with Pax2, Lim1/2, Lbx1, and Bhlhb5 supports a dI6 character. Scale bar: 10 μm. Insets show higher magnifications of respective areas. Scale bar: 5 μm.

    Journal: Life Science Alliance

    Article Title: Neuron-specific inactivation of Wt1 alters locomotion in mice and changes interneuron composition in the spinal cord

    doi: 10.26508/lsa.201800106

    Figure Lengend Snippet: Characterization of Wt1+ neurons in the developing spinal cord. (A) Schematic illustration and Wt1 immunolabelling analysis of a transverse section (12 μm) from E12.5 spinal cord showing the position of Wt1+ neurons (red) in the mantle zone of the developing spinal cord. Stippled line represents the border between the ventricular and mantle zones. Scale bar: 50 μm. (B) Plot showing the average cell number of Wt1+ neurons per 12 μm spinal cord section from different embryonic and postnatal stages. Wt1+ neurons are first found at E12.5 and decrease in cell number postnatally. Data expressed as mean ± SD. n = 12–20 embryos. (C) Determination of the birthdate of Wt1+ neurons by BrdU proliferation assay. Proliferating cells situated in the ventricular zone were labelled by BrdU incorporation at different embryonic stages (E9.5, E10.5, and E11.5). Additional immunolabelling of these cells for Wt1 and BrdU at E12.5 revealed that prospective Wt1 -expressing cells still proliferate at E9.5 and at E10.5 but not at E11.5. Scale bar: 10 μm. Insets show higher magnifications of respective areas. Scale bar: 5 μm. (D) Schematic illustration of an E12.5 spinal cord section with markers and their occurrence in different neuron populations. These markers were used to establish the origin of Wt1+ neurons as dI6 neurons (red). (E) Immunolabelling of Wt1+ neurons with markers present in dI6 and adjacent interneuron populations. The partly overlapping location of Wt1 with Pax2, Lim1/2, Lbx1, and Bhlhb5 supports a dI6 character. Scale bar: 10 μm. Insets show higher magnifications of respective areas. Scale bar: 5 μm.

    Article Snippet: For prefixed samples, antigen retrieval was performed by incubation in sub-boiling 10 mM sodium citrate buffer (pH 6.0) for 30 min. After blocking with 10% goat serum and 2% BSA in PBS-T (postfix or prefix), the sections were incubated with primary antibodies (in blocking solution) using the following dilutions: gBhlhb5 1:50 (Santa Cruz Biotechnology, Inc.), BrdU 1:100 (abcam), shChx10 1:100 (abcam), gpDmrt3 1:5,000 (custom made [ ]), mEvx1 1:100 (1:3,000 prefix) (Developmental Studies Hybridoma Bank, University of Iowa), chGFP 1:1,000 prefix (abcam), mGFP 1:100 (Santa Cruz Biotechnology, Inc.), rFoxP2 1:800 (abcam), mIslet1/2 1:50 (Developmental Studies Hybridoma Bank, University of Iowa), gpLbx1 1:20,000 (gift from C. Birchmeier, MDC), Lim1/2 1:50 (Developmental Studies Hybridoma Bank, University of Iowa), NeuN 1:500 (Merck), rbPax2 1:50 (Thermo Fisher Scientific), rbLmx1b 1:100 (gift from R. Witzgall, University of Regensburg), and rbWt1 1:100 (Santa Cruz Biotechnology, Inc.).

    Techniques: Proliferation Assay, BrdU Incorporation Assay, Expressing

    FGF-induced limbs are innervated by flank level motoneurones, which are responsive to its anterio-posterior orientation. (A) Schematic diagram of stage 13/14 chick embryo showing location of the implanted FGF-4 bead in the presumptive flank (red dot). (B) Whole-mount immunohistochemistry with 3A10 showing axons from flank levels of the spinal cord enter the additional limb (arrowheads). (C) Transverse section of the spinal cord showing HRP-labelled motoneurones on the side of the FGF-induced additional limb at flank levels (arrowhead). (D,E) Transverse section of a stage 26 chick embryonic spinal cord at wing bud level counterstained with DAPI (D) and reacted with the LIM1/2 antibody (E). The LMC L which expresses LIM1 is outlined by dots. Additional staining outside the LMC L is due to LIM2-positive neurons. (F,G) Transverse section of a stage 26 chick embryonic spinal cord at the level of the additional limb counterstained with DAPI (F) and reacted with the LIM1/2 antibody (G). LIM1 is not expressed by the MMC motoneurones (arrowed). (H) Whole-mount antibody staining with 3A10 showing reversed anterio-posterior orientation of the major nerve bundles in the additional bud (asterix and arrows). A and A′= anterior; P and P′= posterior.

    Journal: Journal of Anatomy

    Article Title: The innervation of FGF-induced additional limbs in the chick embryo

    doi: 10.1046/j.1469-7580.2003.00131.x

    Figure Lengend Snippet: FGF-induced limbs are innervated by flank level motoneurones, which are responsive to its anterio-posterior orientation. (A) Schematic diagram of stage 13/14 chick embryo showing location of the implanted FGF-4 bead in the presumptive flank (red dot). (B) Whole-mount immunohistochemistry with 3A10 showing axons from flank levels of the spinal cord enter the additional limb (arrowheads). (C) Transverse section of the spinal cord showing HRP-labelled motoneurones on the side of the FGF-induced additional limb at flank levels (arrowhead). (D,E) Transverse section of a stage 26 chick embryonic spinal cord at wing bud level counterstained with DAPI (D) and reacted with the LIM1/2 antibody (E). The LMC L which expresses LIM1 is outlined by dots. Additional staining outside the LMC L is due to LIM2-positive neurons. (F,G) Transverse section of a stage 26 chick embryonic spinal cord at the level of the additional limb counterstained with DAPI (F) and reacted with the LIM1/2 antibody (G). LIM1 is not expressed by the MMC motoneurones (arrowed). (H) Whole-mount antibody staining with 3A10 showing reversed anterio-posterior orientation of the major nerve bundles in the additional bud (asterix and arrows). A and A′= anterior; P and P′= posterior.

    Article Snippet: Sections were incubated in a 1 : 50 dilution of the LIM1/2 antibody.

    Techniques: Immunohistochemistry, Staining

    Lgr5 is expressed in the early female reproductive tract during embryogenesis. a RNA ISH for Lgr5 in coelomic epithelium at E12.0. b The Lgr5-2A-EGFP mouse model employed to evaluate endogenous Lgr5 expression. c Co-IF for Lgr5-EGFP and Lim1 in coelomic epithelium at E12.0. d Confocal z-stack image of a whole-mount E12.5 Müllerian duct (highlighted by the red dashed line). Yellow box indicates the region magnified in e . e Endogenous EGFP fluorescence in E12.5 Lgr5-2A-EGFP mouse at Md. f Immunostaining for Lgr5-EGFP and E-cadherin in P0 uterus. Dashed red lines indicate Md, and dashed black or white lines indicate Wd, respectively. Md, Müllerian duct; Wd, Wolffian duct; Scale bars, 50 μm. All images are representative of three independent mice.

    Journal: Nature Communications

    Article Title: Neonatal Wnt-dependent Lgr5 positive stem cells are essential for uterine gland development

    doi: 10.1038/s41467-019-13363-3

    Figure Lengend Snippet: Lgr5 is expressed in the early female reproductive tract during embryogenesis. a RNA ISH for Lgr5 in coelomic epithelium at E12.0. b The Lgr5-2A-EGFP mouse model employed to evaluate endogenous Lgr5 expression. c Co-IF for Lgr5-EGFP and Lim1 in coelomic epithelium at E12.0. d Confocal z-stack image of a whole-mount E12.5 Müllerian duct (highlighted by the red dashed line). Yellow box indicates the region magnified in e . e Endogenous EGFP fluorescence in E12.5 Lgr5-2A-EGFP mouse at Md. f Immunostaining for Lgr5-EGFP and E-cadherin in P0 uterus. Dashed red lines indicate Md, and dashed black or white lines indicate Wd, respectively. Md, Müllerian duct; Wd, Wolffian duct; Scale bars, 50 μm. All images are representative of three independent mice.

    Article Snippet: The following primary antibodies were employed: mouse anti-Lim1 (1:100; DSHB, 4F2), rabbit anti-Foxa2 (1:400; Cell Signaling, 8186), rabbit anti-K8 (1:400; Abcam, ab53280), rabbit anti-vimentin (1:1000; Abcam, ab92547), mouse anti-E-cadherin (1:200; BD Transduction Laboratories, 610181), rabbit anti-cleaved Caspase3 (1:200; Cell Signaling, 9661), rabbit anti-Ki67 (1:200; ThermoFisher, MA5-14520), rabbit anti-LIF (1:200; Origene, TA321468), mouse anti-Ki67 (1:200; BD Transduction Laboratories, 550609), chicken anti-GFP (1:100; Abcam, ab13970), rabbit anti-GFP (1:200; Cell Signaling, 2956S), rabbit anti-RFP (1:200; Rockland, 600-401-379), mouse anti-RFP (1:100; Abcam, ab125244).

    Techniques: In Situ Hybridization, Expressing, Fluorescence, Immunostaining, Mouse Assay

    Embryonic Lgr5+ populations are stem/progenitor cells for the female reproductive tract. a The Lgr5-2A-CreERT2; R26-tdTomato mouse model employed to trace Lgr5 + cell-derived progeny. b , c Short-term lineage tracing in the developing reproductive tract induced at E11.5. Co-IF for tdTomato and Lim1 on E12.5 genital ducts shows tdTom + cells in both the Lim1 + Md and the adjacent Lim1 − Wd cells (white dashed lines) ( b ) and reveals expansion of Lim1 + /tdTom + tracing exclusively within the Md at E13.5 ( c ). Yellow arrows indicate single tdTom + cells at E12.5. d Long-term lineage tracing in the female reproductive tract induced from E11.5 to P90. Immunostaining for tdTomato reveals a major contribution of embryonic Lgr5 + cells to the epithelia of the adult oviduct, uterus and vagina. Red arrows indicate glandular cells. Scale bars, 50 μm. All images are representative of three independent mice.

    Journal: Nature Communications

    Article Title: Neonatal Wnt-dependent Lgr5 positive stem cells are essential for uterine gland development

    doi: 10.1038/s41467-019-13363-3

    Figure Lengend Snippet: Embryonic Lgr5+ populations are stem/progenitor cells for the female reproductive tract. a The Lgr5-2A-CreERT2; R26-tdTomato mouse model employed to trace Lgr5 + cell-derived progeny. b , c Short-term lineage tracing in the developing reproductive tract induced at E11.5. Co-IF for tdTomato and Lim1 on E12.5 genital ducts shows tdTom + cells in both the Lim1 + Md and the adjacent Lim1 − Wd cells (white dashed lines) ( b ) and reveals expansion of Lim1 + /tdTom + tracing exclusively within the Md at E13.5 ( c ). Yellow arrows indicate single tdTom + cells at E12.5. d Long-term lineage tracing in the female reproductive tract induced from E11.5 to P90. Immunostaining for tdTomato reveals a major contribution of embryonic Lgr5 + cells to the epithelia of the adult oviduct, uterus and vagina. Red arrows indicate glandular cells. Scale bars, 50 μm. All images are representative of three independent mice.

    Article Snippet: The following primary antibodies were employed: mouse anti-Lim1 (1:100; DSHB, 4F2), rabbit anti-Foxa2 (1:400; Cell Signaling, 8186), rabbit anti-K8 (1:400; Abcam, ab53280), rabbit anti-vimentin (1:1000; Abcam, ab92547), mouse anti-E-cadherin (1:200; BD Transduction Laboratories, 610181), rabbit anti-cleaved Caspase3 (1:200; Cell Signaling, 9661), rabbit anti-Ki67 (1:200; ThermoFisher, MA5-14520), rabbit anti-LIF (1:200; Origene, TA321468), mouse anti-Ki67 (1:200; BD Transduction Laboratories, 550609), chicken anti-GFP (1:100; Abcam, ab13970), rabbit anti-GFP (1:200; Cell Signaling, 2956S), rabbit anti-RFP (1:200; Rockland, 600-401-379), mouse anti-RFP (1:100; Abcam, ab125244).

    Techniques: Derivative Assay, Immunostaining, Mouse Assay

    Knockdown of Meis1 reduces the expression of Foxn4 and Lim1+2. Chick retinas were electroporated with shMeis1-1 or shControl construct at E4. Transfected retina tissues were harvested at E7, sectioned, and immunostained for cell specific antibodies: Foxn4 and Lim1+2 (green). RFP + cells show a dramatic reduction of Foxn4 and Lim1+2 expression in the shMeis1-1 (B,D) transfected cells, but not in the shControl transfected cells (A,C). Double labeled cells (Foxn4 + /RFP + or Lim1+2 + /RFP + ) were indicated by arrowheads, while arrows represent RFP + cells that were negative with Foxn4 or Lim1+2 staining. A histogram (E) shows that there was a dramatic reduction of the percentage of RFP+ cells with Foxn4 and Lim 1+2 staining in shMeis1-1 transfected population. Error bars represent standard error of the mean. Each histogram represents the mean ± s.d.; n ≥3. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar: 20 µm.

    Journal: Biology Open

    Article Title: Meis1 regulates Foxn4 expression during retinal progenitor cell differentiation

    doi: 10.1242/bio.20132279

    Figure Lengend Snippet: Knockdown of Meis1 reduces the expression of Foxn4 and Lim1+2. Chick retinas were electroporated with shMeis1-1 or shControl construct at E4. Transfected retina tissues were harvested at E7, sectioned, and immunostained for cell specific antibodies: Foxn4 and Lim1+2 (green). RFP + cells show a dramatic reduction of Foxn4 and Lim1+2 expression in the shMeis1-1 (B,D) transfected cells, but not in the shControl transfected cells (A,C). Double labeled cells (Foxn4 + /RFP + or Lim1+2 + /RFP + ) were indicated by arrowheads, while arrows represent RFP + cells that were negative with Foxn4 or Lim1+2 staining. A histogram (E) shows that there was a dramatic reduction of the percentage of RFP+ cells with Foxn4 and Lim 1+2 staining in shMeis1-1 transfected population. Error bars represent standard error of the mean. Each histogram represents the mean ± s.d.; n ≥3. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar: 20 µm.

    Article Snippet: Primary antibodies and dilutions used were as follows: goat or rabbit anti-GFP (1:500, Abcam), mouse anti-Foxn4 (1:1000, Aviva), mouse anti-Lim1+2 (1:40, 4F2 supernatant, DSHB), mouse anti-Brn3a (1:200, Millipore), mouse anti-NeuN (1:1000, Millipore), mouse anti-Visinin (1:20, 7G4 supernatant, DSHB), and goat anti-Meis1/2 (1:250, Santa Cruz).

    Techniques: Expressing, Construct, Transfection, Labeling, Staining

    CR4.2 directs GFP expression in Foxn4 + cells and differentiating horizontal cells. Chick retinas were electroporated with either the control CAG-GFP construct or CR4.2-βGP-GFP (CR4.2-GFP) construct at embryonic day 4 (E4). Transfected retinas were harvested at E6, E7, and E8 during development, sectioned, and immunostained for GFP (green), Foxn4 (red, panels A–F), and Lim1+2 (red, panels G–L). (A–F) Double labeled cells (boxed regions) are shown in higher magnification on the right (indicated by arrowheads; arrows point to Foxn4-negative cells). (M,N) Quantification of double labeled cells (GFP + /Foxn4 + or GFP + /Lim1+2 + ). Error bars represent standard error of the mean. Data represent the mean ± s.d.; n ≥3. ONBL, outer neuroblastic layer; INBL, inner neuroblastic layer; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar: 20 µm.

    Journal: Biology Open

    Article Title: Meis1 regulates Foxn4 expression during retinal progenitor cell differentiation

    doi: 10.1242/bio.20132279

    Figure Lengend Snippet: CR4.2 directs GFP expression in Foxn4 + cells and differentiating horizontal cells. Chick retinas were electroporated with either the control CAG-GFP construct or CR4.2-βGP-GFP (CR4.2-GFP) construct at embryonic day 4 (E4). Transfected retinas were harvested at E6, E7, and E8 during development, sectioned, and immunostained for GFP (green), Foxn4 (red, panels A–F), and Lim1+2 (red, panels G–L). (A–F) Double labeled cells (boxed regions) are shown in higher magnification on the right (indicated by arrowheads; arrows point to Foxn4-negative cells). (M,N) Quantification of double labeled cells (GFP + /Foxn4 + or GFP + /Lim1+2 + ). Error bars represent standard error of the mean. Data represent the mean ± s.d.; n ≥3. ONBL, outer neuroblastic layer; INBL, inner neuroblastic layer; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar: 20 µm.

    Article Snippet: Primary antibodies and dilutions used were as follows: goat or rabbit anti-GFP (1:500, Abcam), mouse anti-Foxn4 (1:1000, Aviva), mouse anti-Lim1+2 (1:40, 4F2 supernatant, DSHB), mouse anti-Brn3a (1:200, Millipore), mouse anti-NeuN (1:1000, Millipore), mouse anti-Visinin (1:20, 7G4 supernatant, DSHB), and goat anti-Meis1/2 (1:250, Santa Cruz).

    Techniques: Expressing, Construct, Transfection, Labeling

    Expression patterns of FGF8 , FGFR3 and PEA3 , and ERK1/2 activity in WD. (A-J) In situ hybridization to show expression of LIM1 (A,B; a marker for WD), FGFR3 (C,D), FGF8 (E-H′) and PEA3 (I,J) mRNA in E1.5/HH10 and E2/HH13 embryos. Arrowheads (A-D,I,J) indicate leader cells. The anterior border of the FGF8- expressing area, including L-IMM and PSM, was juxtaposed to the leader cells with a slight overlap (G′,H′, arrowheads). (K,L) Whole-mount immunostaining for diphosphorylated ERK1/2 (pERK) in E1.5/HH10 (K) and E2/HH13 (L) embryos. Arrowheads indicate leader cells. (M) E2/HH13 embryo stained with antibodies for pERK (red) and LIM1 protein (green). Signals for pERK were more intense in leader cells (white arrowheads) than in rear cells (black arrowheads). (N,O) Transverse sections of E2/HH13 embryos immunostained for pERK showing leader cells (O) and rear cells (N). (P) Diagram illustrating the relative positions of FGF signal activation in WD (red) and caudal regression of the FGF8 -expressing area (purple). Scale bars: 100 μm in M; 20 μm in N.

    Journal: Development (Cambridge, England)

    Article Title: FGF8 coordinates tissue elongation and cell epithelialization during early kidney tubulogenesis

    doi: 10.1242/dev.122408

    Figure Lengend Snippet: Expression patterns of FGF8 , FGFR3 and PEA3 , and ERK1/2 activity in WD. (A-J) In situ hybridization to show expression of LIM1 (A,B; a marker for WD), FGFR3 (C,D), FGF8 (E-H′) and PEA3 (I,J) mRNA in E1.5/HH10 and E2/HH13 embryos. Arrowheads (A-D,I,J) indicate leader cells. The anterior border of the FGF8- expressing area, including L-IMM and PSM, was juxtaposed to the leader cells with a slight overlap (G′,H′, arrowheads). (K,L) Whole-mount immunostaining for diphosphorylated ERK1/2 (pERK) in E1.5/HH10 (K) and E2/HH13 (L) embryos. Arrowheads indicate leader cells. (M) E2/HH13 embryo stained with antibodies for pERK (red) and LIM1 protein (green). Signals for pERK were more intense in leader cells (white arrowheads) than in rear cells (black arrowheads). (N,O) Transverse sections of E2/HH13 embryos immunostained for pERK showing leader cells (O) and rear cells (N). (P) Diagram illustrating the relative positions of FGF signal activation in WD (red) and caudal regression of the FGF8 -expressing area (purple). Scale bars: 100 μm in M; 20 μm in N.

    Article Snippet: For DAB (3,3′-diaminobenzidine; WAKO) staining, specimens were washed with TBST and incubated with DAB/PBST for 20 min, then reacted with a 1:2000 dilution of 30% H2 O2 for 5 min. For double staining of LIM1 protein and pERK, 1:200 anti-LIM1 mouse monoclonal antibody (4F2; DSHB) was used along with the anti-pERK antibody.

    Techniques: Expressing, Activity Assay, In Situ Hybridization, Marker, Immunostaining, Staining, Activation Assay

    Loss of HCs in Oc1 -null retinas occurs at early developmental stages. A–H , Immunolabeling for Lim1 and Prox1 at E14.5. Lim1 and Prox1 are expressed in newly generated HCs located at the boundary between the GCL and the NBL ( A , C , E , G ). Prox1

    Journal: The Journal of Neuroscience

    Article Title: Onecut1 Is Essential for Horizontal Cell Genesis and Retinal Integrity

    doi: 10.1523/JNEUROSCI.0116-13.2013

    Figure Lengend Snippet: Loss of HCs in Oc1 -null retinas occurs at early developmental stages. A–H , Immunolabeling for Lim1 and Prox1 at E14.5. Lim1 and Prox1 are expressed in newly generated HCs located at the boundary between the GCL and the NBL ( A , C , E , G ). Prox1

    Article Snippet: Other antibodies used in these studies include: rabbit anti-Lim1 (1:100; Millipore, AB3200), mouse anti-Prox1 (1:200; Millipore, MAB5654), rabbit anti-Sox9 (1:500; Millipore, AB5535), rabbit anti-Pgp9.5 (1:500; Millipore, AB1761), rabbit anti-cone arrestin (CAR) (1:500; Millipore, AB15282), guinea pig anti-doublecortin (Dcx) (1:500; Millipore, AB2253), guinea pig anti-Ptf1a (1:400; Dr. Jane Johnson, Utah Southwestern Medical Center), rabbit anti-Ptf1a (1:800; Dr. Helena Edlund, Umeå University), mouse anti-Bassoon (1:100; Enzo Life Sciences, ADI-VAM-PS003-D), and rabbit anti-GFP (1:150; Abcam, AB290).

    Techniques: Immunolabeling, Generated

    Oc1 and Ptf1a are sufficient to specify HC fate. Retinal sections from embryos electroporated with plasmid constructs expressing GFP, Oc1, and Ptf1a were double immunolabeled with anti-GFP (green) and anti-Lim1 or anti-Prox1 (red). A–D , In retinas

    Journal: The Journal of Neuroscience

    Article Title: Onecut1 Is Essential for Horizontal Cell Genesis and Retinal Integrity

    doi: 10.1523/JNEUROSCI.0116-13.2013

    Figure Lengend Snippet: Oc1 and Ptf1a are sufficient to specify HC fate. Retinal sections from embryos electroporated with plasmid constructs expressing GFP, Oc1, and Ptf1a were double immunolabeled with anti-GFP (green) and anti-Lim1 or anti-Prox1 (red). A–D , In retinas

    Article Snippet: Other antibodies used in these studies include: rabbit anti-Lim1 (1:100; Millipore, AB3200), mouse anti-Prox1 (1:200; Millipore, MAB5654), rabbit anti-Sox9 (1:500; Millipore, AB5535), rabbit anti-Pgp9.5 (1:500; Millipore, AB1761), rabbit anti-cone arrestin (CAR) (1:500; Millipore, AB15282), guinea pig anti-doublecortin (Dcx) (1:500; Millipore, AB2253), guinea pig anti-Ptf1a (1:400; Dr. Jane Johnson, Utah Southwestern Medical Center), rabbit anti-Ptf1a (1:800; Dr. Helena Edlund, Umeå University), mouse anti-Bassoon (1:100; Enzo Life Sciences, ADI-VAM-PS003-D), and rabbit anti-GFP (1:150; Abcam, AB290).

    Techniques: Plasmid Preparation, Construct, Expressing, Immunolabeling