anti-inos antibody Santa Cruz Biotechnology Search Results


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  • 95
    Millipore antibodies against inos
    Antibodies Against Inos, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology inducible nitric oxide synthase inos
    Dose-dependent effect of barley sprouts extract on nitric oxide (NO) generation and protein expression of inducible nitric oxide <t>synthase</t> <t>(iNOS)</t> and cyclooxygenase (COX)-2 in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. ( A ) Cell viability was determined after treatment with barley sprouts extract for 24 h. Cells were pretreated with barley sprouts extract for 1 h before treatment with 200 ng/mL LPS for 24 h; ( B ) NO generation in medium and ( C ) protein expression of iNOS and COX2 in whole cell lysates; ( D ) Quantitative analysis of blots. Each value is mean ± standard deviation (SD) of triplicates in three independent experiments. Values with different letters are significantly different by analysis of variance (ANOVA) followed by Newman–Keuls multiple range test ( p
    Inducible Nitric Oxide Synthase Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti inducible nitric oxide synthase
    Dose-dependent effect of barley sprouts extract on nitric oxide (NO) generation and protein expression of inducible nitric oxide <t>synthase</t> <t>(iNOS)</t> and cyclooxygenase (COX)-2 in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. ( A ) Cell viability was determined after treatment with barley sprouts extract for 24 h. Cells were pretreated with barley sprouts extract for 1 h before treatment with 200 ng/mL LPS for 24 h; ( B ) NO generation in medium and ( C ) protein expression of iNOS and COX2 in whole cell lysates; ( D ) Quantitative analysis of blots. Each value is mean ± standard deviation (SD) of triplicates in three independent experiments. Values with different letters are significantly different by analysis of variance (ANOVA) followed by Newman–Keuls multiple range test ( p
    Anti Inducible Nitric Oxide Synthase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology polyclonal rabbit anti inducible nitric oxide synthase inos
    Dose-dependent effect of barley sprouts extract on nitric oxide (NO) generation and protein expression of inducible nitric oxide <t>synthase</t> <t>(iNOS)</t> and cyclooxygenase (COX)-2 in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. ( A ) Cell viability was determined after treatment with barley sprouts extract for 24 h. Cells were pretreated with barley sprouts extract for 1 h before treatment with 200 ng/mL LPS for 24 h; ( B ) NO generation in medium and ( C ) protein expression of iNOS and COX2 in whole cell lysates; ( D ) Quantitative analysis of blots. Each value is mean ± standard deviation (SD) of triplicates in three independent experiments. Values with different letters are significantly different by analysis of variance (ANOVA) followed by Newman–Keuls multiple range test ( p
    Polyclonal Rabbit Anti Inducible Nitric Oxide Synthase Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Santa Cruz Biotechnology anti inducible nitric oxide synthase anti inos isoform nitros oxide
    Dose-dependent effect of barley sprouts extract on nitric oxide (NO) generation and protein expression of inducible nitric oxide <t>synthase</t> <t>(iNOS)</t> and cyclooxygenase (COX)-2 in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. ( A ) Cell viability was determined after treatment with barley sprouts extract for 24 h. Cells were pretreated with barley sprouts extract for 1 h before treatment with 200 ng/mL LPS for 24 h; ( B ) NO generation in medium and ( C ) protein expression of iNOS and COX2 in whole cell lysates; ( D ) Quantitative analysis of blots. Each value is mean ± standard deviation (SD) of triplicates in three independent experiments. Values with different letters are significantly different by analysis of variance (ANOVA) followed by Newman–Keuls multiple range test ( p
    Anti Inducible Nitric Oxide Synthase Anti Inos Isoform Nitros Oxide, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology rabbit anti inducible nitric oxide synthase inos
    Dose-dependent effect of barley sprouts extract on nitric oxide (NO) generation and protein expression of inducible nitric oxide <t>synthase</t> <t>(iNOS)</t> and cyclooxygenase (COX)-2 in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. ( A ) Cell viability was determined after treatment with barley sprouts extract for 24 h. Cells were pretreated with barley sprouts extract for 1 h before treatment with 200 ng/mL LPS for 24 h; ( B ) NO generation in medium and ( C ) protein expression of iNOS and COX2 in whole cell lysates; ( D ) Quantitative analysis of blots. Each value is mean ± standard deviation (SD) of triplicates in three independent experiments. Values with different letters are significantly different by analysis of variance (ANOVA) followed by Newman–Keuls multiple range test ( p
    Rabbit Anti Inducible Nitric Oxide Synthase Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology phycoerythrin pe conjugated anti inducible nitric oxide synthase
    Dose-dependent effect of barley sprouts extract on nitric oxide (NO) generation and protein expression of inducible nitric oxide <t>synthase</t> <t>(iNOS)</t> and cyclooxygenase (COX)-2 in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. ( A ) Cell viability was determined after treatment with barley sprouts extract for 24 h. Cells were pretreated with barley sprouts extract for 1 h before treatment with 200 ng/mL LPS for 24 h; ( B ) NO generation in medium and ( C ) protein expression of iNOS and COX2 in whole cell lysates; ( D ) Quantitative analysis of blots. Each value is mean ± standard deviation (SD) of triplicates in three independent experiments. Values with different letters are significantly different by analysis of variance (ANOVA) followed by Newman–Keuls multiple range test ( p
    Phycoerythrin Pe Conjugated Anti Inducible Nitric Oxide Synthase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 80/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology mouse anti inducible nitric oxide synthase monoclonal antibody
    Effect of P2Y12R on LPS-stimulated BV-2 microglial morphological changes and inflammatory cytokine production. a Double immunofluorescence staining images of <t>iNOS</t> with Iba-1 in BV-2 cells stimulated with LPS and pretreated with MRS2395/clopidogrel (1.8 μM). Scale bar: 20 μm. b Representative Western blot showing iNOS expression in LPS-stimulated BV-2 cells pretreated with the <t>P2Y12</t> antagonists MRS2395 (20 μM) and clopidogrel (0.18 and 1.8 μM). The band intensity is presented relative to that of β-actin. Data are presented as mean ± SEM of three independent experiments; ** p
    Mouse Anti Inducible Nitric Oxide Synthase Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 78/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology mouse anti human inducible nitric oxide synthase inos
    Effect of P2Y12R on LPS-stimulated BV-2 microglial morphological changes and inflammatory cytokine production. a Double immunofluorescence staining images of <t>iNOS</t> with Iba-1 in BV-2 cells stimulated with LPS and pretreated with MRS2395/clopidogrel (1.8 μM). Scale bar: 20 μm. b Representative Western blot showing iNOS expression in LPS-stimulated BV-2 cells pretreated with the <t>P2Y12</t> antagonists MRS2395 (20 μM) and clopidogrel (0.18 and 1.8 μM). The band intensity is presented relative to that of β-actin. Data are presented as mean ± SEM of three independent experiments; ** p
    Mouse Anti Human Inducible Nitric Oxide Synthase Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 81/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology polyclonal rabbit anti rat inducible nitric oxide synthase inos antibody
    Effect of P2Y12R on LPS-stimulated BV-2 microglial morphological changes and inflammatory cytokine production. a Double immunofluorescence staining images of <t>iNOS</t> with Iba-1 in BV-2 cells stimulated with LPS and pretreated with MRS2395/clopidogrel (1.8 μM). Scale bar: 20 μm. b Representative Western blot showing iNOS expression in LPS-stimulated BV-2 cells pretreated with the <t>P2Y12</t> antagonists MRS2395 (20 μM) and clopidogrel (0.18 and 1.8 μM). The band intensity is presented relative to that of β-actin. Data are presented as mean ± SEM of three independent experiments; ** p
    Polyclonal Rabbit Anti Rat Inducible Nitric Oxide Synthase Inos Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology goat polyclonal anti inducible nitric oxide synthase
    Effect of P2Y12R on LPS-stimulated BV-2 microglial morphological changes and inflammatory cytokine production. a Double immunofluorescence staining images of <t>iNOS</t> with Iba-1 in BV-2 cells stimulated with LPS and pretreated with MRS2395/clopidogrel (1.8 μM). Scale bar: 20 μm. b Representative Western blot showing iNOS expression in LPS-stimulated BV-2 cells pretreated with the <t>P2Y12</t> antagonists MRS2395 (20 μM) and clopidogrel (0.18 and 1.8 μM). The band intensity is presented relative to that of β-actin. Data are presented as mean ± SEM of three independent experiments; ** p
    Goat Polyclonal Anti Inducible Nitric Oxide Synthase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 81/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology rabbit polyclonal anti inducible nitric oxide synthase
    mGSTA4-4 Expression and <t>iNOS</t> upregulation in mGSTA4 -transfected MS1 cells. Panel A: mGSTA4-4 expression by Western blot analysis. 50 μg cell lysates were loaded on 4–12% SDS-PAGE. The primary <t>polyclonal</t> rabbit-anti-mGSTA4-4 antibodies
    Rabbit Polyclonal Anti Inducible Nitric Oxide Synthase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 80/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti mouse inducible nitric oxide synthases
    mGSTA4-4 Expression and <t>iNOS</t> upregulation in mGSTA4 -transfected MS1 cells. Panel A: mGSTA4-4 expression by Western blot analysis. 50 μg cell lysates were loaded on 4–12% SDS-PAGE. The primary <t>polyclonal</t> rabbit-anti-mGSTA4-4 antibodies
    Anti Mouse Inducible Nitric Oxide Synthases, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology mouse anti inducible nitric oxide synthases inos
    mGSTA4-4 Expression and <t>iNOS</t> upregulation in mGSTA4 -transfected MS1 cells. Panel A: mGSTA4-4 expression by Western blot analysis. 50 μg cell lysates were loaded on 4–12% SDS-PAGE. The primary <t>polyclonal</t> rabbit-anti-mGSTA4-4 antibodies
    Mouse Anti Inducible Nitric Oxide Synthases Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti inducible nitric oxide no synthase synthase inos igg
    mGSTA4-4 Expression and <t>iNOS</t> upregulation in mGSTA4 -transfected MS1 cells. Panel A: mGSTA4-4 expression by Western blot analysis. 50 μg cell lysates were loaded on 4–12% SDS-PAGE. The primary <t>polyclonal</t> rabbit-anti-mGSTA4-4 antibodies
    Anti Inducible Nitric Oxide No Synthase Synthase Inos Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology polyclonal rabbit anti mouse inducible nitric oxide synthase inos
    Inhibiting TNFα and <t>iNOS</t> production by human marrow stromal cell treatment in SOD1 transgenic mice. Lumbar spinal cord tissues from wild-type mice, PBS-treated and human marrow stromal cell (hMSC)-treated transgenic mice at the age of 11 weeks are assayed for inducible nitric oxide <t>synthase</t> (iNOS) protein expression by Immunoblot and TNFα level by ELISA. ( A ) Representative immunoblots for iNOS and β-actin of the lumbar spinal cord from PBS-treated transgenic mice (1), wild-type mice (2) and hMSC-treated transgenic mice (3). ( B ) Quantitative evaluation through optical densitometry of iNOS blot and relation to β-actin blot. The levels of iNOS in Cu/Zn superoxide dismutase 1 (SOD1) transgenic mice receiving hMSC transplantation were significantly reduced compared with those in vehicle-treated mice (* P
    Polyclonal Rabbit Anti Mouse Inducible Nitric Oxide Synthase Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti inos antibody
    Laser (632.8nm) suppressed Aβ-induced expressions of <t>IL-1β</t> and <t>iNOS</t> in astrocytes
    Anti Inos Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology goat monoclonal anti inducible nitric oxide synthase antibody
    Laser (632.8nm) suppressed Aβ-induced expressions of <t>IL-1β</t> and <t>iNOS</t> in astrocytes
    Goat Monoclonal Anti Inducible Nitric Oxide Synthase Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology rabbit igg anti mouse inducible nitric oxide synthase inos enzyme
    Th17-associated cytokines regulate the IFN-γ production and inducible nitric oxide <t>synthase</t> enzyme <t>(iNOS)</t> expression during the experiment of virulent yeast strain 18 of Paracoccidioides brasiliensis (Pb18) infection. (A,B) C57BL/6, IL-6 −/− , IL-23 −/− , and IL-17 receptor A (IL-17RA) −/− mice were infected (intravenously) with 1 × 10 6 Pb18 yeast cells. (A) IFN-γ levels were measured by enzyme-linked immunosorbent assay in the lung homogenate at 15 and 30 days postinfection (dpi). (B) Immunohistochemistry was performed in lung tissue to analyze the iNOS expression at 30 dpi. (C) The percentage of the pulmonary area containing iNOS + cells is indicated. Lung sections were stained for iNOS at 30 dpi using immunohistochemistry. Scale bar: 50 µm. Similar results were obtained in three independent experiments. Data represent the mean ± SEM of five mice. Uninfected mice are represented by the dashed line. * p
    Rabbit Igg Anti Mouse Inducible Nitric Oxide Synthase Inos Enzyme, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 81/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology rabbit anti mouse inducible nitric oxide synthase inos
    Th17-associated cytokines regulate the IFN-γ production and inducible nitric oxide <t>synthase</t> enzyme <t>(iNOS)</t> expression during the experiment of virulent yeast strain 18 of Paracoccidioides brasiliensis (Pb18) infection. (A,B) C57BL/6, IL-6 −/− , IL-23 −/− , and IL-17 receptor A (IL-17RA) −/− mice were infected (intravenously) with 1 × 10 6 Pb18 yeast cells. (A) IFN-γ levels were measured by enzyme-linked immunosorbent assay in the lung homogenate at 15 and 30 days postinfection (dpi). (B) Immunohistochemistry was performed in lung tissue to analyze the iNOS expression at 30 dpi. (C) The percentage of the pulmonary area containing iNOS + cells is indicated. Lung sections were stained for iNOS at 30 dpi using immunohistochemistry. Scale bar: 50 µm. Similar results were obtained in three independent experiments. Data represent the mean ± SEM of five mice. Uninfected mice are represented by the dashed line. * p
    Rabbit Anti Mouse Inducible Nitric Oxide Synthase Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology polyclonal anti inos
    Th17-associated cytokines regulate the IFN-γ production and inducible nitric oxide <t>synthase</t> enzyme <t>(iNOS)</t> expression during the experiment of virulent yeast strain 18 of Paracoccidioides brasiliensis (Pb18) infection. (A,B) C57BL/6, IL-6 −/− , IL-23 −/− , and IL-17 receptor A (IL-17RA) −/− mice were infected (intravenously) with 1 × 10 6 Pb18 yeast cells. (A) IFN-γ levels were measured by enzyme-linked immunosorbent assay in the lung homogenate at 15 and 30 days postinfection (dpi). (B) Immunohistochemistry was performed in lung tissue to analyze the iNOS expression at 30 dpi. (C) The percentage of the pulmonary area containing iNOS + cells is indicated. Lung sections were stained for iNOS at 30 dpi using immunohistochemistry. Scale bar: 50 µm. Similar results were obtained in three independent experiments. Data represent the mean ± SEM of five mice. Uninfected mice are represented by the dashed line. * p
    Polyclonal Anti Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 79/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti inos pe
    Th17-associated cytokines regulate the IFN-γ production and inducible nitric oxide <t>synthase</t> enzyme <t>(iNOS)</t> expression during the experiment of virulent yeast strain 18 of Paracoccidioides brasiliensis (Pb18) infection. (A,B) C57BL/6, IL-6 −/− , IL-23 −/− , and IL-17 receptor A (IL-17RA) −/− mice were infected (intravenously) with 1 × 10 6 Pb18 yeast cells. (A) IFN-γ levels were measured by enzyme-linked immunosorbent assay in the lung homogenate at 15 and 30 days postinfection (dpi). (B) Immunohistochemistry was performed in lung tissue to analyze the iNOS expression at 30 dpi. (C) The percentage of the pulmonary area containing iNOS + cells is indicated. Lung sections were stained for iNOS at 30 dpi using immunohistochemistry. Scale bar: 50 µm. Similar results were obtained in three independent experiments. Data represent the mean ± SEM of five mice. Uninfected mice are represented by the dashed line. * p
    Anti Inos Pe, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 77/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti inos ab m 19
    Th17-associated cytokines regulate the IFN-γ production and inducible nitric oxide <t>synthase</t> enzyme <t>(iNOS)</t> expression during the experiment of virulent yeast strain 18 of Paracoccidioides brasiliensis (Pb18) infection. (A,B) C57BL/6, IL-6 −/− , IL-23 −/− , and IL-17 receptor A (IL-17RA) −/− mice were infected (intravenously) with 1 × 10 6 Pb18 yeast cells. (A) IFN-γ levels were measured by enzyme-linked immunosorbent assay in the lung homogenate at 15 and 30 days postinfection (dpi). (B) Immunohistochemistry was performed in lung tissue to analyze the iNOS expression at 30 dpi. (C) The percentage of the pulmonary area containing iNOS + cells is indicated. Lung sections were stained for iNOS at 30 dpi using immunohistochemistry. Scale bar: 50 µm. Similar results were obtained in three independent experiments. Data represent the mean ± SEM of five mice. Uninfected mice are represented by the dashed line. * p
    Anti Inos Ab M 19, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology monoclonal anti inos ab
    cSCK-pa 100 mediated silencing of <t>iNOS</t> expression by siRNA. Anti-iNOS siRNA481 (100 nM) was transfected into mouse monocyte-macrophage RAW264.7 cells using PAEA 128 - b -PS 40 cSCK-pa 100 at different N/P ratios (for N/P=1, cSCK 0.62 μg/mL),
    Monoclonal Anti Inos Ab, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology mouse anti inos antibody
    cSCK-pa 100 mediated silencing of <t>iNOS</t> expression by siRNA. Anti-iNOS siRNA481 (100 nM) was transfected into mouse monocyte-macrophage RAW264.7 cells using PAEA 128 - b -PS 40 cSCK-pa 100 at different N/P ratios (for N/P=1, cSCK 0.62 μg/mL),
    Mouse Anti Inos Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology rabbit anti inos antibody
    Loss of Mφ function in disseminated tularemia. Wild-type or caspase-3-deficient mice were infected with 48 CFU of KU49, and the expression of <t>F4/80,</t> TNF-α, and <t>iNOS</t> in their spleens or livers 3 or 4 days p.i. were determined by immunoperoxidase techniques. Bars, 100 μm. The frequencies of F4/80 + , TNF-α + , and iNOS + cells on day 4 p.i. in the spleens of caspase-3-deficient mice were significantly greater than those of wild-type mice (*, P
    Rabbit Anti Inos Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology goat anti inos
    Normal migration of macrophages and DCs in the absence of CCR5. (A) C57BL/6 and CCR5 −/− mice were infected with 2,000 L. monocytogenes organisms, and spleens were harvested at 2 days postinfection. Spleen sections were stained with antibodies to L. monocytogenes (green), CD169 (red), Mac-3 (red), and <t>DEC-205</t> (red). RP, red pulp; WP, white pulp; MZ, marginal zone. (B) Spleens were harvested from wild-type and CCR5 −/− mice at 2 days postinfection and digested with collagenase, and the expression of CD11c, CD11b, and Mac-3 was analyzed by flow cytometry. The percentages of CD11b high , CD11b int , CD11b low CD11c high , and CD11b neg CD11c high (upper panels) and of CD11b int Mac-3 high and CD11b high Mac-3 low (lower panels) cells are indicated. Representative dot plots for three mice per group are shown. (C) Spleen sections from mice infected with L. monocytogenes for 3 days were stained for <t>iNOS</t> (red) and counterstained with hematoxylin. Each subpanel in panels A and C is a representative staining with three mice per group analyzed, and the experiments were repeated twice.
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    Santa Cruz Biotechnology anti inos polycloncal antibody pab
    Normal migration of macrophages and DCs in the absence of CCR5. (A) C57BL/6 and CCR5 −/− mice were infected with 2,000 L. monocytogenes organisms, and spleens were harvested at 2 days postinfection. Spleen sections were stained with antibodies to L. monocytogenes (green), CD169 (red), Mac-3 (red), and <t>DEC-205</t> (red). RP, red pulp; WP, white pulp; MZ, marginal zone. (B) Spleens were harvested from wild-type and CCR5 −/− mice at 2 days postinfection and digested with collagenase, and the expression of CD11c, CD11b, and Mac-3 was analyzed by flow cytometry. The percentages of CD11b high , CD11b int , CD11b low CD11c high , and CD11b neg CD11c high (upper panels) and of CD11b int Mac-3 high and CD11b high Mac-3 low (lower panels) cells are indicated. Representative dot plots for three mice per group are shown. (C) Spleen sections from mice infected with L. monocytogenes for 3 days were stained for <t>iNOS</t> (red) and counterstained with hematoxylin. Each subpanel in panels A and C is a representative staining with three mice per group analyzed, and the experiments were repeated twice.
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    Santa Cruz Biotechnology rabbit polyclonal anti inos antibody
    Normal migration of macrophages and DCs in the absence of CCR5. (A) C57BL/6 and CCR5 −/− mice were infected with 2,000 L. monocytogenes organisms, and spleens were harvested at 2 days postinfection. Spleen sections were stained with antibodies to L. monocytogenes (green), CD169 (red), Mac-3 (red), and <t>DEC-205</t> (red). RP, red pulp; WP, white pulp; MZ, marginal zone. (B) Spleens were harvested from wild-type and CCR5 −/− mice at 2 days postinfection and digested with collagenase, and the expression of CD11c, CD11b, and Mac-3 was analyzed by flow cytometry. The percentages of CD11b high , CD11b int , CD11b low CD11c high , and CD11b neg CD11c high (upper panels) and of CD11b int Mac-3 high and CD11b high Mac-3 low (lower panels) cells are indicated. Representative dot plots for three mice per group are shown. (C) Spleen sections from mice infected with L. monocytogenes for 3 days were stained for <t>iNOS</t> (red) and counterstained with hematoxylin. Each subpanel in panels A and C is a representative staining with three mice per group analyzed, and the experiments were repeated twice.
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    Santa Cruz Biotechnology pe conjugated anti inos
    Gr1 + (Ly6C+) inflammatory monocytes in the lamina propria express <t>iNOS,</t> TNFα, and IL-12 but not dendritic cell markers
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    Gr1 + (Ly6C+) inflammatory monocytes in the lamina propria express <t>iNOS,</t> TNFα, and IL-12 but not dendritic cell markers
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    Image Search Results


    Dose-dependent effect of barley sprouts extract on nitric oxide (NO) generation and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. ( A ) Cell viability was determined after treatment with barley sprouts extract for 24 h. Cells were pretreated with barley sprouts extract for 1 h before treatment with 200 ng/mL LPS for 24 h; ( B ) NO generation in medium and ( C ) protein expression of iNOS and COX2 in whole cell lysates; ( D ) Quantitative analysis of blots. Each value is mean ± standard deviation (SD) of triplicates in three independent experiments. Values with different letters are significantly different by analysis of variance (ANOVA) followed by Newman–Keuls multiple range test ( p

    Journal: Nutrients

    Article Title: Barley Sprouts Extract Attenuates Alcoholic Fatty Liver Injury in Mice by Reducing Inflammatory Response

    doi: 10.3390/nu8070440

    Figure Lengend Snippet: Dose-dependent effect of barley sprouts extract on nitric oxide (NO) generation and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. ( A ) Cell viability was determined after treatment with barley sprouts extract for 24 h. Cells were pretreated with barley sprouts extract for 1 h before treatment with 200 ng/mL LPS for 24 h; ( B ) NO generation in medium and ( C ) protein expression of iNOS and COX2 in whole cell lysates; ( D ) Quantitative analysis of blots. Each value is mean ± standard deviation (SD) of triplicates in three independent experiments. Values with different letters are significantly different by analysis of variance (ANOVA) followed by Newman–Keuls multiple range test ( p

    Article Snippet: The membranes were incubated with TBST containing 5% milk and the primary antibodies against anti-inducible nitric oxide synthase (iNOS), anti-cyclooxygenase (COX)-2, and β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA).

    Techniques: Expressing, Standard Deviation

    Plasma markers of oxidative stress and nitric oxide synthase activity. Urinary excretion of the oxidative stress markers 8‐ OH ‐ dG (A), 8,12‐iso‐ iPF 2α‐ VI (B), and its metabolite 2,3‐dinor‐8‐iso iPF 2α (C) were increased in A 3 +/+ mice after UNX in combination with HS ( UNX ‐ HS ) but were not significantly changed in the A 3 −/− mice. UNX ‐ HS caused significant increase of plasma citrulline/arginine ratio in A 3 −/− mice (D) and significant reduction of plasma ornithine/arginine ratio in A 3 +/+ mice only (E). Baseline levels of both citrulline/arginine and ornithine/arginine ratios were lower in the A 3 −/− mice compared with A 3 +/+ mice. A 3 −/− mice had significantly lower plasma ADMA and SDMA levels (F and G). Although UNX ‐ HS did not influence circulating ADMA and SDMA levels, the SDMA level after UNX ‐ HS was significantly lower in A 3 −/− compared with A 3 +/+ mice. Data are shown as mean± SEM , * P

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Genetic Abrogation of Adenosine A3 Receptor Prevents Uninephrectomy and High Salt–Induced Hypertension

    doi: 10.1161/JAHA.116.003868

    Figure Lengend Snippet: Plasma markers of oxidative stress and nitric oxide synthase activity. Urinary excretion of the oxidative stress markers 8‐ OH ‐ dG (A), 8,12‐iso‐ iPF 2α‐ VI (B), and its metabolite 2,3‐dinor‐8‐iso iPF 2α (C) were increased in A 3 +/+ mice after UNX in combination with HS ( UNX ‐ HS ) but were not significantly changed in the A 3 −/− mice. UNX ‐ HS caused significant increase of plasma citrulline/arginine ratio in A 3 −/− mice (D) and significant reduction of plasma ornithine/arginine ratio in A 3 +/+ mice only (E). Baseline levels of both citrulline/arginine and ornithine/arginine ratios were lower in the A 3 −/− mice compared with A 3 +/+ mice. A 3 −/− mice had significantly lower plasma ADMA and SDMA levels (F and G). Although UNX ‐ HS did not influence circulating ADMA and SDMA levels, the SDMA level after UNX ‐ HS was significantly lower in A 3 −/− compared with A 3 +/+ mice. Data are shown as mean± SEM , * P

    Article Snippet: Membranes were treated with blocking solution (5% nonfat dry milk, 20 mmol/L Tris base, 150 mmol/L NaCl, 0.1% Tween‐20, pH 7.6) and then incubated with primary antibodies gp91phox/Nox2 (BD Biosciences); p67phox (Cell Signaling/BioNordika); or p22phox, p47phox, or inducible nitric oxide synthase (iNOS; Santa Cruz Biotechnology) overnight at 4°C.

    Techniques: Activity Assay, Mouse Assay

    Inducible nitric oxide synthase (iNOS) immunoreactivity is increased following MDMA administration. ( A – D ) Representative images (light microscopy, 40×) of iNOS immunoreactivity in the frontal cortex of rats receiving ( A ) saline (CTRL) and of rats receiving MDMA and sacrificed after ( B ) 6 h, ( C ) 16 h, and ( D ) 24 h from its administration. ( E – H ) Representative images (light microscopy, 40×) of endothelial nitric oxide synthase (eNOS) immunoreactivity in the frontal cortex of rats receiving ( E ) saline (CTRL) and of rats receiving MDMA and sacrificed after ( F ) 6 h, ( G ) 16 h, and ( H ) 24 h from its administration. ( I – L ) Representative images (light microscopy, 40×) of neuronal nitric oxide synthase (nNOS) immunoreactivity in the frontal cortex of rats receiving ( I ) saline (CTRL) and of rats receiving MDMA and sacrificed after ( J ) 6 h, ( K ) 16 h, and ( L ) 24 h from its administration. Scale bar for images in panels ( A – L ) = 50 μm. ( M – O ) Quantification of ( M ) iNOS, ( N ) eNOS, and ( O ) nNOS positive-stained cells/area analyzed in controls (CTRL) and MDMA-exposed rats, sacrificed after 6 h, 16 h, and 24 h from its administration. One-way ANOVA followed by Tukey’s post-hoc test. For iNOS: F = 9.090, *** p

    Journal: International Journal of Molecular Sciences

    Article Title: Increased iNOS and Nitrosative Stress in Dopaminergic Neurons of MDMA-Exposed Rats

    doi: 10.3390/ijms20051242

    Figure Lengend Snippet: Inducible nitric oxide synthase (iNOS) immunoreactivity is increased following MDMA administration. ( A – D ) Representative images (light microscopy, 40×) of iNOS immunoreactivity in the frontal cortex of rats receiving ( A ) saline (CTRL) and of rats receiving MDMA and sacrificed after ( B ) 6 h, ( C ) 16 h, and ( D ) 24 h from its administration. ( E – H ) Representative images (light microscopy, 40×) of endothelial nitric oxide synthase (eNOS) immunoreactivity in the frontal cortex of rats receiving ( E ) saline (CTRL) and of rats receiving MDMA and sacrificed after ( F ) 6 h, ( G ) 16 h, and ( H ) 24 h from its administration. ( I – L ) Representative images (light microscopy, 40×) of neuronal nitric oxide synthase (nNOS) immunoreactivity in the frontal cortex of rats receiving ( I ) saline (CTRL) and of rats receiving MDMA and sacrificed after ( J ) 6 h, ( K ) 16 h, and ( L ) 24 h from its administration. Scale bar for images in panels ( A – L ) = 50 μm. ( M – O ) Quantification of ( M ) iNOS, ( N ) eNOS, and ( O ) nNOS positive-stained cells/area analyzed in controls (CTRL) and MDMA-exposed rats, sacrificed after 6 h, 16 h, and 24 h from its administration. One-way ANOVA followed by Tukey’s post-hoc test. For iNOS: F = 9.090, *** p

    Article Snippet: For this study, 4 μm paraffin-embedded sections of the frontal cortex region were obtained from specimen A, by using an automized microtome (Leica, Cambridge, UK), mounted on 3-amminopropyl-triethoxysilane covered slides (Fluka, Buchs, Switzerland), and dried at 37 °C for 24 h. Brain sections were then deparaffinized through graded alcohols, subjected to epitope retrieval for 15 min and incubated for two hours at room temperature with primary antibodies, diluted in a blocking buffered serum solution containing albumin and fetal bovine serum (Sigma-Aldrich S.R.L., Milan, Italy), raised against NOX2 (1:50, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), NOX1 (1: 250, Abcam, Cambridge, UK), NOX4 (1:100, Abcam), iNOS (1:100, Santa Cruz Biotechnology), eNOS, (1:100, Santa Cruz Biotechnology), nNOS (1:150, Santa Cruz Biotechnology, Inc.), 8OHdG (1:10, JaICA, Shizuoka, Japan), NT (1:600, Santa Cruz Biotechnology, Inc.), DT1 (1:100, Abcam).

    Techniques: Light Microscopy, Staining

    Effect of P2Y12R on LPS-stimulated BV-2 microglial morphological changes and inflammatory cytokine production. a Double immunofluorescence staining images of iNOS with Iba-1 in BV-2 cells stimulated with LPS and pretreated with MRS2395/clopidogrel (1.8 μM). Scale bar: 20 μm. b Representative Western blot showing iNOS expression in LPS-stimulated BV-2 cells pretreated with the P2Y12 antagonists MRS2395 (20 μM) and clopidogrel (0.18 and 1.8 μM). The band intensity is presented relative to that of β-actin. Data are presented as mean ± SEM of three independent experiments; ** p

    Journal: Journal of Neuroinflammation

    Article Title: P2Y12 receptor mediates microglial activation via RhoA/ROCK pathway in the trigeminal nucleus caudalis in a mouse model of chronic migraine

    doi: 10.1186/s12974-019-1603-4

    Figure Lengend Snippet: Effect of P2Y12R on LPS-stimulated BV-2 microglial morphological changes and inflammatory cytokine production. a Double immunofluorescence staining images of iNOS with Iba-1 in BV-2 cells stimulated with LPS and pretreated with MRS2395/clopidogrel (1.8 μM). Scale bar: 20 μm. b Representative Western blot showing iNOS expression in LPS-stimulated BV-2 cells pretreated with the P2Y12 antagonists MRS2395 (20 μM) and clopidogrel (0.18 and 1.8 μM). The band intensity is presented relative to that of β-actin. Data are presented as mean ± SEM of three independent experiments; ** p

    Article Snippet: Coverslips were blocked with 5% goat/donkey serum containing 0.3% Triton X-100 for 30 min at room temperature, and were then incubated with following primary antibodies overnight at 4 °C: rabbit anti-P2Y12 (1:500, Anaspec Inc), goat anti-Iba1 (1:200, Abcam), and mouse anti-iNOS (1:50, Santa Cruz).

    Techniques: Double Immunofluorescence Staining, Western Blot, Expressing

    mGSTA4-4 Expression and iNOS upregulation in mGSTA4 -transfected MS1 cells. Panel A: mGSTA4-4 expression by Western blot analysis. 50 μg cell lysates were loaded on 4–12% SDS-PAGE. The primary polyclonal rabbit-anti-mGSTA4-4 antibodies

    Journal: Toxicology and applied pharmacology

    Article Title: Endothelial glutathione-S-transferase A4-4 protects against oxidative stress and modulates iNOS expression through NF-?B translocation

    doi: 10.1016/j.taap.2008.03.018

    Figure Lengend Snippet: mGSTA4-4 Expression and iNOS upregulation in mGSTA4 -transfected MS1 cells. Panel A: mGSTA4-4 expression by Western blot analysis. 50 μg cell lysates were loaded on 4–12% SDS-PAGE. The primary polyclonal rabbit-anti-mGSTA4-4 antibodies

    Article Snippet: Lesions and adjacent sections of selected lesions were immunostained for 4-HNE and iNOS with goat anti-HNE antiserum (Alpha Diagnostic, San Antonio, TX), and rabbit anti-iNOS polyclonal antibodies (Santa Cruz Biotechnology., Santa Cruz, CA).

    Techniques: Expressing, Transfection, Western Blot, SDS Page

    Inhibiting TNFα and iNOS production by human marrow stromal cell treatment in SOD1 transgenic mice. Lumbar spinal cord tissues from wild-type mice, PBS-treated and human marrow stromal cell (hMSC)-treated transgenic mice at the age of 11 weeks are assayed for inducible nitric oxide synthase (iNOS) protein expression by Immunoblot and TNFα level by ELISA. ( A ) Representative immunoblots for iNOS and β-actin of the lumbar spinal cord from PBS-treated transgenic mice (1), wild-type mice (2) and hMSC-treated transgenic mice (3). ( B ) Quantitative evaluation through optical densitometry of iNOS blot and relation to β-actin blot. The levels of iNOS in Cu/Zn superoxide dismutase 1 (SOD1) transgenic mice receiving hMSC transplantation were significantly reduced compared with those in vehicle-treated mice (* P

    Journal: Journal of Neuroinflammation

    Article Title: Human marrow stromal cells reduce microglial activation to protect motor neurons in a transgenic mouse model of amyotrophic lateral sclerosis

    doi: 10.1186/1742-2094-10-52

    Figure Lengend Snippet: Inhibiting TNFα and iNOS production by human marrow stromal cell treatment in SOD1 transgenic mice. Lumbar spinal cord tissues from wild-type mice, PBS-treated and human marrow stromal cell (hMSC)-treated transgenic mice at the age of 11 weeks are assayed for inducible nitric oxide synthase (iNOS) protein expression by Immunoblot and TNFα level by ELISA. ( A ) Representative immunoblots for iNOS and β-actin of the lumbar spinal cord from PBS-treated transgenic mice (1), wild-type mice (2) and hMSC-treated transgenic mice (3). ( B ) Quantitative evaluation through optical densitometry of iNOS blot and relation to β-actin blot. The levels of iNOS in Cu/Zn superoxide dismutase 1 (SOD1) transgenic mice receiving hMSC transplantation were significantly reduced compared with those in vehicle-treated mice (* P

    Article Snippet: The blots were blocked with 5% (w/v) defatted milk in TBS-Tween20 (pH 8.0) for 1 hour at room temperature and then incubated overnight at 4°C with primary antibodies: polyclonal rabbit anti-mouse inducible nitric oxide synthase (iNOS) (1:1,000; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and mouse anti-actin (1:1,000; Santa Cruz Biotechnology).

    Techniques: Transgenic Assay, Mouse Assay, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Transplantation Assay

    Laser (632.8nm) suppressed Aβ-induced expressions of IL-1β and iNOS in astrocytes

    Journal: Neuroscience

    Article Title: Low energy laser light (632.8 nm) suppresses amyloid-? peptide-induced oxidative and inflammatory responses in astrocytes

    doi: 10.1016/j.neuroscience.2010.09.025

    Figure Lengend Snippet: Laser (632.8nm) suppressed Aβ-induced expressions of IL-1β and iNOS in astrocytes

    Article Snippet: Membranes were blocked for 1 h with 5% (w/v) nonfat dry milk in Tris-buffered saline containing 0.1% (v/v) Tween 20 (TBST) and were incubated overnight at 4°C in 3% (w/v) BSA with 0.02% (w/v) sodium azide in TBST with p-cPLA2 or cPLA2 antibodies (1:1000 dilution; Cell Signaling Technology, Beverly, MA), anti-IL-1β antibody (1:1000 dilution; R & D Systems, Minneapolis, MN), anti-iNOS antibody (1:1000 dilution; Santa Cruz Biotechnology, Santa Cruz, CA) and β-actin antibody (Sigma, St. Louis, MO).

    Techniques:

    Effects of DS on phosphorylation of Akt and eNOS in brain tissues. Phosphorylation of Akt (p-Akt) and eNOS (p-eNOS) in brain tissues of saline- (Con) and DS-treated mice at 60 min after ischemia. Akt, p-Akt, eNOS, p-eNOS, iNOS, and nNOS protein levels were analyzed by Western blotting ( N = 4). DS promoted Akt and eNOS phosphorylation in both ischemic (ipsilateral, Ipsil) and nonischemic regions (contralateral, Cont) of the brain compared with control.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: The Traditional Herbal Medicine, Dangkwisoo-San, Prevents Cerebral Ischemic Injury through Nitric Oxide-Dependent Mechanisms

    doi: 10.1155/2011/718302

    Figure Lengend Snippet: Effects of DS on phosphorylation of Akt and eNOS in brain tissues. Phosphorylation of Akt (p-Akt) and eNOS (p-eNOS) in brain tissues of saline- (Con) and DS-treated mice at 60 min after ischemia. Akt, p-Akt, eNOS, p-eNOS, iNOS, and nNOS protein levels were analyzed by Western blotting ( N = 4). DS promoted Akt and eNOS phosphorylation in both ischemic (ipsilateral, Ipsil) and nonischemic regions (contralateral, Cont) of the brain compared with control.

    Article Snippet: Immunoblot analysis was performed with anti-eNOS and anti-phospho-eNOS (pS1177) antibodies (BD Biosciences, San Jose, CA), anti-Akt and anti-phospho-Akt (Ser 473) antibodies (Cell signaling, Danvers, MA), anti-nNOS and anti-iNOS antibodies (Santa Cruz Biotechnology, Santa Cruz, CA) followed by incubation with secondary antibody conjugated with horseradish peroxidase.

    Techniques: Mouse Assay, Western Blot

    Th17-associated cytokines regulate the IFN-γ production and inducible nitric oxide synthase enzyme (iNOS) expression during the experiment of virulent yeast strain 18 of Paracoccidioides brasiliensis (Pb18) infection. (A,B) C57BL/6, IL-6 −/− , IL-23 −/− , and IL-17 receptor A (IL-17RA) −/− mice were infected (intravenously) with 1 × 10 6 Pb18 yeast cells. (A) IFN-γ levels were measured by enzyme-linked immunosorbent assay in the lung homogenate at 15 and 30 days postinfection (dpi). (B) Immunohistochemistry was performed in lung tissue to analyze the iNOS expression at 30 dpi. (C) The percentage of the pulmonary area containing iNOS + cells is indicated. Lung sections were stained for iNOS at 30 dpi using immunohistochemistry. Scale bar: 50 µm. Similar results were obtained in three independent experiments. Data represent the mean ± SEM of five mice. Uninfected mice are represented by the dashed line. * p

    Journal: Frontiers in Immunology

    Article Title: Th17-Inducing Cytokines IL-6 and IL-23 Are Crucial for Granuloma Formation during Experimental Paracoccidioidomycosis

    doi: 10.3389/fimmu.2017.00949

    Figure Lengend Snippet: Th17-associated cytokines regulate the IFN-γ production and inducible nitric oxide synthase enzyme (iNOS) expression during the experiment of virulent yeast strain 18 of Paracoccidioides brasiliensis (Pb18) infection. (A,B) C57BL/6, IL-6 −/− , IL-23 −/− , and IL-17 receptor A (IL-17RA) −/− mice were infected (intravenously) with 1 × 10 6 Pb18 yeast cells. (A) IFN-γ levels were measured by enzyme-linked immunosorbent assay in the lung homogenate at 15 and 30 days postinfection (dpi). (B) Immunohistochemistry was performed in lung tissue to analyze the iNOS expression at 30 dpi. (C) The percentage of the pulmonary area containing iNOS + cells is indicated. Lung sections were stained for iNOS at 30 dpi using immunohistochemistry. Scale bar: 50 µm. Similar results were obtained in three independent experiments. Data represent the mean ± SEM of five mice. Uninfected mice are represented by the dashed line. * p

    Article Snippet: Briefly, slides were incubated with anti-mouse IgG as control or rabbit IgG anti-mouse inducible nitric oxide synthase (iNOS) enzyme (Santa Cruz Biotechnology, Santa Cruz, CA, USA) diluted 100 times in PBS 0.01% saponin.

    Techniques: Expressing, Infection, Mouse Assay, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Staining

    cSCK-pa 100 mediated silencing of iNOS expression by siRNA. Anti-iNOS siRNA481 (100 nM) was transfected into mouse monocyte-macrophage RAW264.7 cells using PAEA 128 - b -PS 40 cSCK-pa 100 at different N/P ratios (for N/P=1, cSCK 0.62 μg/mL),

    Journal: Nucleic Acid Therapeutics

    Article Title: Efficient Protection and Transfection of Small Interfering RNA by Cationic Shell-Crosslinked Knedel-Like Nanoparticles

    doi: 10.1089/nat.2012.0390

    Figure Lengend Snippet: cSCK-pa 100 mediated silencing of iNOS expression by siRNA. Anti-iNOS siRNA481 (100 nM) was transfected into mouse monocyte-macrophage RAW264.7 cells using PAEA 128 - b -PS 40 cSCK-pa 100 at different N/P ratios (for N/P=1, cSCK 0.62 μg/mL),

    Article Snippet: The separated proteins were then transferred onto polyvinylidene difluoride membrane (Amersham Hybond™ -P, GE Healthcare). iNOS was detected by using monoclonal anti-iNOS antibody NOS2(C-11) at 1:200 (Santa Cruz Biotechnology, Inc.). β-actin was detected using mouse anti- β-actin monoclonal antibody at 1:20,000 (GenScript Corporation).

    Techniques: Expressing, Transfection

    iNOS mRNA silencing by cSCK•siRNA•GALA nanocomplex in RAW264.7 cells. (A) cytoxicity of PAEA 160 -b-PS 30 cSCK-pa 100 after 24 h in RAW cells that had been induced for 24 hours with LPS/γ-IFN as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium

    Journal: Nucleic Acid Therapeutics

    Article Title: Efficient Protection and Transfection of Small Interfering RNA by Cationic Shell-Crosslinked Knedel-Like Nanoparticles

    doi: 10.1089/nat.2012.0390

    Figure Lengend Snippet: iNOS mRNA silencing by cSCK•siRNA•GALA nanocomplex in RAW264.7 cells. (A) cytoxicity of PAEA 160 -b-PS 30 cSCK-pa 100 after 24 h in RAW cells that had been induced for 24 hours with LPS/γ-IFN as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium

    Article Snippet: The separated proteins were then transferred onto polyvinylidene difluoride membrane (Amersham Hybond™ -P, GE Healthcare). iNOS was detected by using monoclonal anti-iNOS antibody NOS2(C-11) at 1:200 (Santa Cruz Biotechnology, Inc.). β-actin was detected using mouse anti- β-actin monoclonal antibody at 1:20,000 (GenScript Corporation).

    Techniques:

    Endocytosis inhibition and tracking studies. (A) Inhibition experiments. iNOS-induced RAW 264.7 cells were incubated with cytochalasin D (Cyto-D) (average of 2–50 μM), chlorpromazine (CPZ) (20 μM) and methyl-beta-cyclodextrin,

    Journal: Nucleic Acid Therapeutics

    Article Title: Efficient Protection and Transfection of Small Interfering RNA by Cationic Shell-Crosslinked Knedel-Like Nanoparticles

    doi: 10.1089/nat.2012.0390

    Figure Lengend Snippet: Endocytosis inhibition and tracking studies. (A) Inhibition experiments. iNOS-induced RAW 264.7 cells were incubated with cytochalasin D (Cyto-D) (average of 2–50 μM), chlorpromazine (CPZ) (20 μM) and methyl-beta-cyclodextrin,

    Article Snippet: The separated proteins were then transferred onto polyvinylidene difluoride membrane (Amersham Hybond™ -P, GE Healthcare). iNOS was detected by using monoclonal anti-iNOS antibody NOS2(C-11) at 1:200 (Santa Cruz Biotechnology, Inc.). β-actin was detected using mouse anti- β-actin monoclonal antibody at 1:20,000 (GenScript Corporation).

    Techniques: Inhibition, Incubation

    Loss of Mφ function in disseminated tularemia. Wild-type or caspase-3-deficient mice were infected with 48 CFU of KU49, and the expression of F4/80, TNF-α, and iNOS in their spleens or livers 3 or 4 days p.i. were determined by immunoperoxidase techniques. Bars, 100 μm. The frequencies of F4/80 + , TNF-α + , and iNOS + cells on day 4 p.i. in the spleens of caspase-3-deficient mice were significantly greater than those of wild-type mice (*, P

    Journal: Infection and Immunity

    Article Title: Francisella tularensis Induces Extensive Caspase-3 Activation and Apoptotic Cell Death in the Tissues of Infected Mice ▿

    doi: 10.1128/IAI.00246-09

    Figure Lengend Snippet: Loss of Mφ function in disseminated tularemia. Wild-type or caspase-3-deficient mice were infected with 48 CFU of KU49, and the expression of F4/80, TNF-α, and iNOS in their spleens or livers 3 or 4 days p.i. were determined by immunoperoxidase techniques. Bars, 100 μm. The frequencies of F4/80 + , TNF-α + , and iNOS + cells on day 4 p.i. in the spleens of caspase-3-deficient mice were significantly greater than those of wild-type mice (*, P

    Article Snippet: The following primary antibodies were used in the present study: goat anti-activated caspase-1 (Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-activated caspase-3 (Cell Signaling Technology, Danvers, MA), rabbit anti-activated capase-9 (Cell Signaling), rabbit anti- F. tularensis (BD Diagnostic Systems, Sparks, MD), rat anti-F4/80 (AbD Serotec, Raleigh, NC), rabbit anti-iNOS (Santa Cruz), and rat anti- tumor necrosis factor alpha (anti-TNF-α; BD Biosciences, San Jose, CA).

    Techniques: Mouse Assay, Infection, Expressing

    Normal migration of macrophages and DCs in the absence of CCR5. (A) C57BL/6 and CCR5 −/− mice were infected with 2,000 L. monocytogenes organisms, and spleens were harvested at 2 days postinfection. Spleen sections were stained with antibodies to L. monocytogenes (green), CD169 (red), Mac-3 (red), and DEC-205 (red). RP, red pulp; WP, white pulp; MZ, marginal zone. (B) Spleens were harvested from wild-type and CCR5 −/− mice at 2 days postinfection and digested with collagenase, and the expression of CD11c, CD11b, and Mac-3 was analyzed by flow cytometry. The percentages of CD11b high , CD11b int , CD11b low CD11c high , and CD11b neg CD11c high (upper panels) and of CD11b int Mac-3 high and CD11b high Mac-3 low (lower panels) cells are indicated. Representative dot plots for three mice per group are shown. (C) Spleen sections from mice infected with L. monocytogenes for 3 days were stained for iNOS (red) and counterstained with hematoxylin. Each subpanel in panels A and C is a representative staining with three mice per group analyzed, and the experiments were repeated twice.

    Journal: Infection and Immunity

    Article Title: Chemokine Receptor 5 Is Dispensable for Innate and Adaptive Immune Responses to Listeria monocytogenes Infection

    doi: 10.1128/IAI.72.2.1057-1064.2004

    Figure Lengend Snippet: Normal migration of macrophages and DCs in the absence of CCR5. (A) C57BL/6 and CCR5 −/− mice were infected with 2,000 L. monocytogenes organisms, and spleens were harvested at 2 days postinfection. Spleen sections were stained with antibodies to L. monocytogenes (green), CD169 (red), Mac-3 (red), and DEC-205 (red). RP, red pulp; WP, white pulp; MZ, marginal zone. (B) Spleens were harvested from wild-type and CCR5 −/− mice at 2 days postinfection and digested with collagenase, and the expression of CD11c, CD11b, and Mac-3 was analyzed by flow cytometry. The percentages of CD11b high , CD11b int , CD11b low CD11c high , and CD11b neg CD11c high (upper panels) and of CD11b int Mac-3 high and CD11b high Mac-3 low (lower panels) cells are indicated. Representative dot plots for three mice per group are shown. (C) Spleen sections from mice infected with L. monocytogenes for 3 days were stained for iNOS (red) and counterstained with hematoxylin. Each subpanel in panels A and C is a representative staining with three mice per group analyzed, and the experiments were repeated twice.

    Article Snippet: The sections were acetone fixed and incubated with goat anti-CCR5 (Santa Cruz Biotechnology, Santa Cruz, Calif.), rat anti-CD169 (SEROTEC), rat anti-Mac-3 (BD Pharmingen, San Diego, Calif.), Difco Listeria O polyserum (Fisher), and rat anti-DEC-205 and goat anti-iNOS (Santa Cruz) antibodies.

    Techniques: Migration, Mouse Assay, Infection, Staining, Expressing, Flow Cytometry, Cytometry

    Gr1 + (Ly6C+) inflammatory monocytes in the lamina propria express iNOS, TNFα, and IL-12 but not dendritic cell markers

    Journal:

    Article Title: Gr1+ (Ly6C+) Inflammatory Monocytes are Required for Mucosal Resistance to the Pathogen Toxoplasma gondii

    doi: 10.1016/j.immuni.2008.05.019

    Figure Lengend Snippet: Gr1 + (Ly6C+) inflammatory monocytes in the lamina propria express iNOS, TNFα, and IL-12 but not dendritic cell markers

    Article Snippet: Thereafter, cells were incubated on ice for 30 min with fluorescently conjugated antibodies for cell surface markers: Cy5-conjugated anti-Gr1 mAb RB6-8C5 (eBioscience, San Diego, CA), PE or APC- conjugated anti-F4/80 mAb A3-1 (AbD serotec, Raleigh, NC), FITC-conjugated hamster anti-mouse p150/90 (CD11c) (eBioscience), PE-conjugated anti-iNOS (M-19, Santa Cruz), FITC-conjugated anti-Ly6C mAb AL-21 (BD bioscience), PE-conjugated anti-Ly6G mAb 1A8 (BD bioscience), or Cy5-conjugated anti-CD11b mAb Mac-1α (eBioscience).

    Techniques: