anti-human syndecan-2-allophycocyanin Search Results


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  • 99
    Thermo Fisher live dead fixable violet dead cell stain kit
    MPA decreases cell proliferation and PBMC response to activation. <t>VK2/E6E7</t> cells were plated in the presence or absence of the indicated concentrations of MPA and after 5 days in culture, the cells were trypsinized and stained for Ki67 expression by flow cytometry analysis (A). PBMC were isolated and treated with PHA and the indicated concentrations of MPA for 3 days and analyzed by flow cytometry (B). Data is expressed as Ki67+ expression in <t>live</t> epithelial (A) and live CD4+ or CD8+ T cell populations (B) relative to mock-treated cells. Results are mean + SEM of three independent experiments (A,B). PBMC were plated in the presence or absence of MPA. At 3 days post-treatment, PBMC were activated with PHA (5 μg/ml) and incubated for an additional 3 days. Supernatants were collected and analyzed for cytokines and chemokines (C). Results are mean + SEM obtained from two different blood donors (C). Asterisks indicate statistically significantly different from mock-treated cells, p
    Live Dead Fixable Violet Dead Cell Stain Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2209 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Santa Cruz Biotechnology syntenin 1
    MPA decreases cell proliferation and PBMC response to activation. <t>VK2/E6E7</t> cells were plated in the presence or absence of the indicated concentrations of MPA and after 5 days in culture, the cells were trypsinized and stained for Ki67 expression by flow cytometry analysis (A). PBMC were isolated and treated with PHA and the indicated concentrations of MPA for 3 days and analyzed by flow cytometry (B). Data is expressed as Ki67+ expression in <t>live</t> epithelial (A) and live CD4+ or CD8+ T cell populations (B) relative to mock-treated cells. Results are mean + SEM of three independent experiments (A,B). PBMC were plated in the presence or absence of MPA. At 3 days post-treatment, PBMC were activated with PHA (5 μg/ml) and incubated for an additional 3 days. Supernatants were collected and analyzed for cytokines and chemokines (C). Results are mean + SEM obtained from two different blood donors (C). Asterisks indicate statistically significantly different from mock-treated cells, p
    Syntenin 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Becton Dickinson alexa fluor 647 rat anti mouse rankl
    MPA decreases cell proliferation and PBMC response to activation. <t>VK2/E6E7</t> cells were plated in the presence or absence of the indicated concentrations of MPA and after 5 days in culture, the cells were trypsinized and stained for Ki67 expression by flow cytometry analysis (A). PBMC were isolated and treated with PHA and the indicated concentrations of MPA for 3 days and analyzed by flow cytometry (B). Data is expressed as Ki67+ expression in <t>live</t> epithelial (A) and live CD4+ or CD8+ T cell populations (B) relative to mock-treated cells. Results are mean + SEM of three independent experiments (A,B). PBMC were plated in the presence or absence of MPA. At 3 days post-treatment, PBMC were activated with PHA (5 μg/ml) and incubated for an additional 3 days. Supernatants were collected and analyzed for cytokines and chemokines (C). Results are mean + SEM obtained from two different blood donors (C). Asterisks indicate statistically significantly different from mock-treated cells, p
    Alexa Fluor 647 Rat Anti Mouse Rankl, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    BioLegend apc anti mouse cd138
    MPA decreases cell proliferation and PBMC response to activation. <t>VK2/E6E7</t> cells were plated in the presence or absence of the indicated concentrations of MPA and after 5 days in culture, the cells were trypsinized and stained for Ki67 expression by flow cytometry analysis (A). PBMC were isolated and treated with PHA and the indicated concentrations of MPA for 3 days and analyzed by flow cytometry (B). Data is expressed as Ki67+ expression in <t>live</t> epithelial (A) and live CD4+ or CD8+ T cell populations (B) relative to mock-treated cells. Results are mean + SEM of three independent experiments (A,B). PBMC were plated in the presence or absence of MPA. At 3 days post-treatment, PBMC were activated with PHA (5 μg/ml) and incubated for an additional 3 days. Supernatants were collected and analyzed for cytokines and chemokines (C). Results are mean + SEM obtained from two different blood donors (C). Asterisks indicate statistically significantly different from mock-treated cells, p
    Apc Anti Mouse Cd138, supplied by BioLegend, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend anti cd45r b220 cy7 apc
    MPA decreases cell proliferation and PBMC response to activation. <t>VK2/E6E7</t> cells were plated in the presence or absence of the indicated concentrations of MPA and after 5 days in culture, the cells were trypsinized and stained for Ki67 expression by flow cytometry analysis (A). PBMC were isolated and treated with PHA and the indicated concentrations of MPA for 3 days and analyzed by flow cytometry (B). Data is expressed as Ki67+ expression in <t>live</t> epithelial (A) and live CD4+ or CD8+ T cell populations (B) relative to mock-treated cells. Results are mean + SEM of three independent experiments (A,B). PBMC were plated in the presence or absence of MPA. At 3 days post-treatment, PBMC were activated with PHA (5 μg/ml) and incubated for an additional 3 days. Supernatants were collected and analyzed for cytokines and chemokines (C). Results are mean + SEM obtained from two different blood donors (C). Asterisks indicate statistically significantly different from mock-treated cells, p
    Anti Cd45r B220 Cy7 Apc, supplied by BioLegend, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    R&D Systems anti human syndecan 3 allophycocyanin
    MPA decreases cell proliferation and PBMC response to activation. <t>VK2/E6E7</t> cells were plated in the presence or absence of the indicated concentrations of MPA and after 5 days in culture, the cells were trypsinized and stained for Ki67 expression by flow cytometry analysis (A). PBMC were isolated and treated with PHA and the indicated concentrations of MPA for 3 days and analyzed by flow cytometry (B). Data is expressed as Ki67+ expression in <t>live</t> epithelial (A) and live CD4+ or CD8+ T cell populations (B) relative to mock-treated cells. Results are mean + SEM of three independent experiments (A,B). PBMC were plated in the presence or absence of MPA. At 3 days post-treatment, PBMC were activated with PHA (5 μg/ml) and incubated for an additional 3 days. Supernatants were collected and analyzed for cytokines and chemokines (C). Results are mean + SEM obtained from two different blood donors (C). Asterisks indicate statistically significantly different from mock-treated cells, p
    Anti Human Syndecan 3 Allophycocyanin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 88/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    R&D Systems anti syndecan 4 antibody
    MPA decreases cell proliferation and PBMC response to activation. <t>VK2/E6E7</t> cells were plated in the presence or absence of the indicated concentrations of MPA and after 5 days in culture, the cells were trypsinized and stained for Ki67 expression by flow cytometry analysis (A). PBMC were isolated and treated with PHA and the indicated concentrations of MPA for 3 days and analyzed by flow cytometry (B). Data is expressed as Ki67+ expression in <t>live</t> epithelial (A) and live CD4+ or CD8+ T cell populations (B) relative to mock-treated cells. Results are mean + SEM of three independent experiments (A,B). PBMC were plated in the presence or absence of MPA. At 3 days post-treatment, PBMC were activated with PHA (5 μg/ml) and incubated for an additional 3 days. Supernatants were collected and analyzed for cytokines and chemokines (C). Results are mean + SEM obtained from two different blood donors (C). Asterisks indicate statistically significantly different from mock-treated cells, p
    Anti Syndecan 4 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    R&D Systems anti glypican 1 antibody
    Prolonged exposure to atheroprotective flow induces expression of syndecan-1 on the apical surface of endothelial cells. A : flow cytometry histograms of syndecan-1, -2, and -4 and <t>glypican-1</t> surface expression on cells cultures under static condition or exposed to atheroprotective or atheroprone shear stress waveform for 7 days and quantitative analysis of flow cytometry data from 3 independent experiments. Values are means ± SE ( n = 3). B : representative immunostaining images of syndecan-1 in cells cultured under static condition or exposed to atheroprotective or atheroprone flow for 7 days. Syndecan-1 is shown in green, and nucleus (DAPI) is shown in red. C : mRNA expression of heparan sulfate protein carriers ( left ) and key enzymes specifically responsible for heparan sulfate chain biosynthesis ( right ). Values are means ± SE ( n = 3).
    Anti Glypican 1 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Becton Dickinson alexa fluor 700 mouse anti human ki 67 antibody
    Prolonged exposure to atheroprotective flow induces expression of syndecan-1 on the apical surface of endothelial cells. A : flow cytometry histograms of syndecan-1, -2, and -4 and <t>glypican-1</t> surface expression on cells cultures under static condition or exposed to atheroprotective or atheroprone shear stress waveform for 7 days and quantitative analysis of flow cytometry data from 3 independent experiments. Values are means ± SE ( n = 3). B : representative immunostaining images of syndecan-1 in cells cultured under static condition or exposed to atheroprotective or atheroprone flow for 7 days. Syndecan-1 is shown in green, and nucleus (DAPI) is shown in red. C : mRNA expression of heparan sulfate protein carriers ( left ) and key enzymes specifically responsible for heparan sulfate chain biosynthesis ( right ). Values are means ± SE ( n = 3).
    Alexa Fluor 700 Mouse Anti Human Ki 67 Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 89/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    BioLegend apc cy7 anti mouse cd138 syndecan 1 antibody
    Prolonged exposure to atheroprotective flow induces expression of syndecan-1 on the apical surface of endothelial cells. A : flow cytometry histograms of syndecan-1, -2, and -4 and <t>glypican-1</t> surface expression on cells cultures under static condition or exposed to atheroprotective or atheroprone shear stress waveform for 7 days and quantitative analysis of flow cytometry data from 3 independent experiments. Values are means ± SE ( n = 3). B : representative immunostaining images of syndecan-1 in cells cultured under static condition or exposed to atheroprotective or atheroprone flow for 7 days. Syndecan-1 is shown in green, and nucleus (DAPI) is shown in red. C : mRNA expression of heparan sulfate protein carriers ( left ) and key enzymes specifically responsible for heparan sulfate chain biosynthesis ( right ). Values are means ± SE ( n = 3).
    Apc Cy7 Anti Mouse Cd138 Syndecan 1 Antibody, supplied by BioLegend, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend apc cy7 anti mouse cd19 antibody
    Prolonged exposure to atheroprotective flow induces expression of syndecan-1 on the apical surface of endothelial cells. A : flow cytometry histograms of syndecan-1, -2, and -4 and <t>glypican-1</t> surface expression on cells cultures under static condition or exposed to atheroprotective or atheroprone shear stress waveform for 7 days and quantitative analysis of flow cytometry data from 3 independent experiments. Values are means ± SE ( n = 3). B : representative immunostaining images of syndecan-1 in cells cultured under static condition or exposed to atheroprotective or atheroprone flow for 7 days. Syndecan-1 is shown in green, and nucleus (DAPI) is shown in red. C : mRNA expression of heparan sulfate protein carriers ( left ) and key enzymes specifically responsible for heparan sulfate chain biosynthesis ( right ). Values are means ± SE ( n = 3).
    Apc Cy7 Anti Mouse Cd19 Antibody, supplied by BioLegend, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend apc cy7 anti mouse cd86
    Prolonged exposure to atheroprotective flow induces expression of syndecan-1 on the apical surface of endothelial cells. A : flow cytometry histograms of syndecan-1, -2, and -4 and <t>glypican-1</t> surface expression on cells cultures under static condition or exposed to atheroprotective or atheroprone shear stress waveform for 7 days and quantitative analysis of flow cytometry data from 3 independent experiments. Values are means ± SE ( n = 3). B : representative immunostaining images of syndecan-1 in cells cultured under static condition or exposed to atheroprotective or atheroprone flow for 7 days. Syndecan-1 is shown in green, and nucleus (DAPI) is shown in red. C : mRNA expression of heparan sulfate protein carriers ( left ) and key enzymes specifically responsible for heparan sulfate chain biosynthesis ( right ). Values are means ± SE ( n = 3).
    Apc Cy7 Anti Mouse Cd86, supplied by BioLegend, used in various techniques. Bioz Stars score: 98/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher cd111
    Prolonged exposure to atheroprotective flow induces expression of syndecan-1 on the apical surface of endothelial cells. A : flow cytometry histograms of syndecan-1, -2, and -4 and <t>glypican-1</t> surface expression on cells cultures under static condition or exposed to atheroprotective or atheroprone shear stress waveform for 7 days and quantitative analysis of flow cytometry data from 3 independent experiments. Values are means ± SE ( n = 3). B : representative immunostaining images of syndecan-1 in cells cultured under static condition or exposed to atheroprotective or atheroprone flow for 7 days. Syndecan-1 is shown in green, and nucleus (DAPI) is shown in red. C : mRNA expression of heparan sulfate protein carriers ( left ) and key enzymes specifically responsible for heparan sulfate chain biosynthesis ( right ). Values are means ± SE ( n = 3).
    Cd111, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    BioLegend cd138 apc cy7
    Prolonged exposure to atheroprotective flow induces expression of syndecan-1 on the apical surface of endothelial cells. A : flow cytometry histograms of syndecan-1, -2, and -4 and <t>glypican-1</t> surface expression on cells cultures under static condition or exposed to atheroprotective or atheroprone shear stress waveform for 7 days and quantitative analysis of flow cytometry data from 3 independent experiments. Values are means ± SE ( n = 3). B : representative immunostaining images of syndecan-1 in cells cultured under static condition or exposed to atheroprotective or atheroprone flow for 7 days. Syndecan-1 is shown in green, and nucleus (DAPI) is shown in red. C : mRNA expression of heparan sulfate protein carriers ( left ) and key enzymes specifically responsible for heparan sulfate chain biosynthesis ( right ). Values are means ± SE ( n = 3).
    Cd138 Apc Cy7, supplied by BioLegend, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend cd270 pe
    Prolonged exposure to atheroprotective flow induces expression of syndecan-1 on the apical surface of endothelial cells. A : flow cytometry histograms of syndecan-1, -2, and -4 and <t>glypican-1</t> surface expression on cells cultures under static condition or exposed to atheroprotective or atheroprone shear stress waveform for 7 days and quantitative analysis of flow cytometry data from 3 independent experiments. Values are means ± SE ( n = 3). B : representative immunostaining images of syndecan-1 in cells cultured under static condition or exposed to atheroprotective or atheroprone flow for 7 days. Syndecan-1 is shown in green, and nucleus (DAPI) is shown in red. C : mRNA expression of heparan sulfate protein carriers ( left ) and key enzymes specifically responsible for heparan sulfate chain biosynthesis ( right ). Values are means ± SE ( n = 3).
    Cd270 Pe, supplied by BioLegend, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson cytofix cytoperm fixation permeabilization solution kit
    Prolonged exposure to atheroprotective flow induces expression of syndecan-1 on the apical surface of endothelial cells. A : flow cytometry histograms of syndecan-1, -2, and -4 and <t>glypican-1</t> surface expression on cells cultures under static condition or exposed to atheroprotective or atheroprone shear stress waveform for 7 days and quantitative analysis of flow cytometry data from 3 independent experiments. Values are means ± SE ( n = 3). B : representative immunostaining images of syndecan-1 in cells cultured under static condition or exposed to atheroprotective or atheroprone flow for 7 days. Syndecan-1 is shown in green, and nucleus (DAPI) is shown in red. C : mRNA expression of heparan sulfate protein carriers ( left ) and key enzymes specifically responsible for heparan sulfate chain biosynthesis ( right ). Values are means ± SE ( n = 3).
    Cytofix Cytoperm Fixation Permeabilization Solution Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 1829 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rabbit anti goat igg
    Prolonged exposure to atheroprotective flow induces expression of syndecan-1 on the apical surface of endothelial cells. A : flow cytometry histograms of syndecan-1, -2, and -4 and <t>glypican-1</t> surface expression on cells cultures under static condition or exposed to atheroprotective or atheroprone shear stress waveform for 7 days and quantitative analysis of flow cytometry data from 3 independent experiments. Values are means ± SE ( n = 3). B : representative immunostaining images of syndecan-1 in cells cultured under static condition or exposed to atheroprotective or atheroprone flow for 7 days. Syndecan-1 is shown in green, and nucleus (DAPI) is shown in red. C : mRNA expression of heparan sulfate protein carriers ( left ) and key enzymes specifically responsible for heparan sulfate chain biosynthesis ( right ). Values are means ± SE ( n = 3).
    Rabbit Anti Goat Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 509 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher fluorescein isothiocyanate fitc mouse anti human cd44v6
    Prolonged exposure to atheroprotective flow induces expression of syndecan-1 on the apical surface of endothelial cells. A : flow cytometry histograms of syndecan-1, -2, and -4 and <t>glypican-1</t> surface expression on cells cultures under static condition or exposed to atheroprotective or atheroprone shear stress waveform for 7 days and quantitative analysis of flow cytometry data from 3 independent experiments. Values are means ± SE ( n = 3). B : representative immunostaining images of syndecan-1 in cells cultured under static condition or exposed to atheroprotective or atheroprone flow for 7 days. Syndecan-1 is shown in green, and nucleus (DAPI) is shown in red. C : mRNA expression of heparan sulfate protein carriers ( left ) and key enzymes specifically responsible for heparan sulfate chain biosynthesis ( right ). Values are means ± SE ( n = 3).
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    Becton Dickinson facscanto
    Prolonged exposure to atheroprotective flow induces expression of syndecan-1 on the apical surface of endothelial cells. A : flow cytometry histograms of syndecan-1, -2, and -4 and <t>glypican-1</t> surface expression on cells cultures under static condition or exposed to atheroprotective or atheroprone shear stress waveform for 7 days and quantitative analysis of flow cytometry data from 3 independent experiments. Values are means ± SE ( n = 3). B : representative immunostaining images of syndecan-1 in cells cultured under static condition or exposed to atheroprotective or atheroprone flow for 7 days. Syndecan-1 is shown in green, and nucleus (DAPI) is shown in red. C : mRNA expression of heparan sulfate protein carriers ( left ) and key enzymes specifically responsible for heparan sulfate chain biosynthesis ( right ). Values are means ± SE ( n = 3).
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    BioLegend pe anti mouse immunoglobulin g igg fc
    Prolonged exposure to atheroprotective flow induces expression of syndecan-1 on the apical surface of endothelial cells. A : flow cytometry histograms of syndecan-1, -2, and -4 and <t>glypican-1</t> surface expression on cells cultures under static condition or exposed to atheroprotective or atheroprone shear stress waveform for 7 days and quantitative analysis of flow cytometry data from 3 independent experiments. Values are means ± SE ( n = 3). B : representative immunostaining images of syndecan-1 in cells cultured under static condition or exposed to atheroprotective or atheroprone flow for 7 days. Syndecan-1 is shown in green, and nucleus (DAPI) is shown in red. C : mRNA expression of heparan sulfate protein carriers ( left ) and key enzymes specifically responsible for heparan sulfate chain biosynthesis ( right ). Values are means ± SE ( n = 3).
    Pe Anti Mouse Immunoglobulin G Igg Fc, supplied by BioLegend, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson phycoerythrin conjugated anti mouse cd138 syndecan
    Prolonged exposure to atheroprotective flow induces expression of syndecan-1 on the apical surface of endothelial cells. A : flow cytometry histograms of syndecan-1, -2, and -4 and <t>glypican-1</t> surface expression on cells cultures under static condition or exposed to atheroprotective or atheroprone shear stress waveform for 7 days and quantitative analysis of flow cytometry data from 3 independent experiments. Values are means ± SE ( n = 3). B : representative immunostaining images of syndecan-1 in cells cultured under static condition or exposed to atheroprotective or atheroprone flow for 7 days. Syndecan-1 is shown in green, and nucleus (DAPI) is shown in red. C : mRNA expression of heparan sulfate protein carriers ( left ) and key enzymes specifically responsible for heparan sulfate chain biosynthesis ( right ). Values are means ± SE ( n = 3).
    Phycoerythrin Conjugated Anti Mouse Cd138 Syndecan, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MPA decreases cell proliferation and PBMC response to activation. VK2/E6E7 cells were plated in the presence or absence of the indicated concentrations of MPA and after 5 days in culture, the cells were trypsinized and stained for Ki67 expression by flow cytometry analysis (A). PBMC were isolated and treated with PHA and the indicated concentrations of MPA for 3 days and analyzed by flow cytometry (B). Data is expressed as Ki67+ expression in live epithelial (A) and live CD4+ or CD8+ T cell populations (B) relative to mock-treated cells. Results are mean + SEM of three independent experiments (A,B). PBMC were plated in the presence or absence of MPA. At 3 days post-treatment, PBMC were activated with PHA (5 μg/ml) and incubated for an additional 3 days. Supernatants were collected and analyzed for cytokines and chemokines (C). Results are mean + SEM obtained from two different blood donors (C). Asterisks indicate statistically significantly different from mock-treated cells, p

    Journal: PLoS ONE

    Article Title: Molecular Mechanisms Linking High Dose Medroxyprogesterone with HIV-1 Risk

    doi: 10.1371/journal.pone.0121135

    Figure Lengend Snippet: MPA decreases cell proliferation and PBMC response to activation. VK2/E6E7 cells were plated in the presence or absence of the indicated concentrations of MPA and after 5 days in culture, the cells were trypsinized and stained for Ki67 expression by flow cytometry analysis (A). PBMC were isolated and treated with PHA and the indicated concentrations of MPA for 3 days and analyzed by flow cytometry (B). Data is expressed as Ki67+ expression in live epithelial (A) and live CD4+ or CD8+ T cell populations (B) relative to mock-treated cells. Results are mean + SEM of three independent experiments (A,B). PBMC were plated in the presence or absence of MPA. At 3 days post-treatment, PBMC were activated with PHA (5 μg/ml) and incubated for an additional 3 days. Supernatants were collected and analyzed for cytokines and chemokines (C). Results are mean + SEM obtained from two different blood donors (C). Asterisks indicate statistically significantly different from mock-treated cells, p

    Article Snippet: To evaluate cell proliferation, VK2/E6E7 were treated with MPA, stained with LIVE/Dead fixable violet dead cell stain kit (Life Technologies), fixed and permeabilized with Cytofix/Cytoperm Fixation/Permeabilization Solution Kit (BD Biosciences), and finally stained with Alexa Fluor 700 mouse anti-human Ki-67 antibody (BD Pharmigen).

    Techniques: Activation Assay, Staining, Expressing, Flow Cytometry, Cytometry, Isolation, Incubation

    MPA upregulates proinflammatory cytokines and chemokines, and syndecans in vaginal epithelial cells. VK2/E6E7 cells were treated with the indicated concentrations of MPA for 5 days. RNA was extracted, converted to cDNA and analyzed by qRT-PCR for gene expression (A,C) and the culture supernatants were analyzed by Luminex for a panel of cytokines and chemokines (B). The cytokine/chemokine concentrations were log10 transformed to reduce skewness and results are presented as mean + SEM (pg/ml) obtained from two independent experiments. Gene expression was quantified relative to RPLPO and is presented as the mean + SEM relative to mock-treated cells (log 10 ) from at least 3 independent experiments (A,C). Alternatively, to examine protein expression, the cells were trypsinized and stained with anti-SDC2-APC or anti-SDC3-APC and analyzed by flow cytometry (D). The results are presented as percentage of APC positive cells after gating on the live cell population and are mean + SEM obtained from two independent experiments (D). Asterisks indicate statistically significantly increased expression compared to mock-treated cells, p

    Journal: PLoS ONE

    Article Title: Molecular Mechanisms Linking High Dose Medroxyprogesterone with HIV-1 Risk

    doi: 10.1371/journal.pone.0121135

    Figure Lengend Snippet: MPA upregulates proinflammatory cytokines and chemokines, and syndecans in vaginal epithelial cells. VK2/E6E7 cells were treated with the indicated concentrations of MPA for 5 days. RNA was extracted, converted to cDNA and analyzed by qRT-PCR for gene expression (A,C) and the culture supernatants were analyzed by Luminex for a panel of cytokines and chemokines (B). The cytokine/chemokine concentrations were log10 transformed to reduce skewness and results are presented as mean + SEM (pg/ml) obtained from two independent experiments. Gene expression was quantified relative to RPLPO and is presented as the mean + SEM relative to mock-treated cells (log 10 ) from at least 3 independent experiments (A,C). Alternatively, to examine protein expression, the cells were trypsinized and stained with anti-SDC2-APC or anti-SDC3-APC and analyzed by flow cytometry (D). The results are presented as percentage of APC positive cells after gating on the live cell population and are mean + SEM obtained from two independent experiments (D). Asterisks indicate statistically significantly increased expression compared to mock-treated cells, p

    Article Snippet: To evaluate cell proliferation, VK2/E6E7 were treated with MPA, stained with LIVE/Dead fixable violet dead cell stain kit (Life Technologies), fixed and permeabilized with Cytofix/Cytoperm Fixation/Permeabilization Solution Kit (BD Biosciences), and finally stained with Alexa Fluor 700 mouse anti-human Ki-67 antibody (BD Pharmigen).

    Techniques: Quantitative RT-PCR, Expressing, Luminex, Transformation Assay, Staining, Flow Cytometry, Cytometry

    Prolonged exposure to atheroprotective flow induces expression of syndecan-1 on the apical surface of endothelial cells. A : flow cytometry histograms of syndecan-1, -2, and -4 and glypican-1 surface expression on cells cultures under static condition or exposed to atheroprotective or atheroprone shear stress waveform for 7 days and quantitative analysis of flow cytometry data from 3 independent experiments. Values are means ± SE ( n = 3). B : representative immunostaining images of syndecan-1 in cells cultured under static condition or exposed to atheroprotective or atheroprone flow for 7 days. Syndecan-1 is shown in green, and nucleus (DAPI) is shown in red. C : mRNA expression of heparan sulfate protein carriers ( left ) and key enzymes specifically responsible for heparan sulfate chain biosynthesis ( right ). Values are means ± SE ( n = 3).

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Hemodynamic shear stress characteristic of atherosclerosis-resistant regions promotes glycocalyx formation in cultured endothelial cells

    doi: 10.1152/ajpcell.00187.2012

    Figure Lengend Snippet: Prolonged exposure to atheroprotective flow induces expression of syndecan-1 on the apical surface of endothelial cells. A : flow cytometry histograms of syndecan-1, -2, and -4 and glypican-1 surface expression on cells cultures under static condition or exposed to atheroprotective or atheroprone shear stress waveform for 7 days and quantitative analysis of flow cytometry data from 3 independent experiments. Values are means ± SE ( n = 3). B : representative immunostaining images of syndecan-1 in cells cultured under static condition or exposed to atheroprotective or atheroprone flow for 7 days. Syndecan-1 is shown in green, and nucleus (DAPI) is shown in red. C : mRNA expression of heparan sulfate protein carriers ( left ) and key enzymes specifically responsible for heparan sulfate chain biosynthesis ( right ). Values are means ± SE ( n = 3).

    Article Snippet: The following primary antibodies were used: anti-syndecan-1 antibody (1:10 dilution, biotin-conjugated mouse IgG1; clone B-A38, Abcam, Cambridge, MA), anti-syndecan-2 antibody (1:10 dilution, allophycocyanin-conjugated rat IgG2B; R & D Systems, Minneapolis, MN), anti-syndecan-4 antibody (1:5 dilution; biotin-conjugated goat IgG, R & D Systems), anti-glypican-1 antibody (1:5 dilution; biotin-conjugated goat IgG, R & D Systems), anti-CD44 antibody (1:5 dilution, biotin-conjugated mouse IgG2B; BD Biosciences, Bedford, MA), anti-heparan sulfate antibody (1:100 dilution, mouse IgM; clone 10E4, US Biological, Swampscott, MA), and anti-chondroitin sulfate antibody (1:50 dilution, mouse IgG2A; BD Pharmingen, San Diego, CA).

    Techniques: Flow Cytometry, Expressing, Cytometry, Immunostaining, Cell Culture