anti-goat igg Search Results


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  • 99
    Vector Laboratories antigoat igg
    Antigoat Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Thermo Fisher anti goat igg ab
    Anti Goat Igg Ab, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore antigoat igg antibody
    Antigoat Igg Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agilent technologies goat igg ab
    Goat Igg Ab, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Santa Cruz Biotechnology anti goat igg secondary abs
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Anti Goat Igg Secondary Abs, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Bio-Rad rabbit antigoat igg
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Rabbit Antigoat Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bio-Rad goat igg1
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Goat Igg1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Nordic-Mubio anti goat igg
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Anti Goat Igg, supplied by Nordic-Mubio, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Abcam donkey f ab 2 antigoat igg h l
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Donkey F Ab 2 Antigoat Igg H L, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Santa Cruz Biotechnology anti igg goat biotinylated ab
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Anti Igg Goat Biotinylated Ab, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Millipore antigoat igg
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Antigoat Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Abcam goat f ab anti mouse igg h l
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Goat F Ab Anti Mouse Igg H L, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Abcam goat f
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Goat F, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    BioLegend goat anti mouse igg ab
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Goat Anti Mouse Igg Ab, supplied by BioLegend, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher goat f ab 2 antihuman igg
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Goat F Ab 2 Antihuman Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bio-Rad igg2a
    Immunization with pCVM-Tag induces a mixed <t>IgG</t> subtype distribution of antibodies to SV40 Tag.
    Igg2a, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 516 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Abcam anti goat antibody
    Immunization with pCVM-Tag induces a mixed <t>IgG</t> subtype distribution of antibodies to SV40 Tag.
    Anti Goat Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bio-Rad polyclonal goat anti feline igg
    Immunization with pCVM-Tag induces a mixed <t>IgG</t> subtype distribution of antibodies to SV40 Tag.
    Polyclonal Goat Anti Feline Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher anti goat immunoglobulin g
    Assessment of damage to peripheral retinal ganglion cells and optic nerves in the mouse eyes that overexpressed BMP2 in conventional outflow tissues. Thirty-six days after the viral infusion, the mouse eyes and the optic nerves were collected. A : An image from the periphery of the flatmounted retinas of transduced mouse eyes that were immunostained with Brn3a <t>immunoglobulin</t> G (IgG) and imaged with epifluorescence microscopy. B : The morphology of the myelinated axons (toluidine blue stained) of mice 36 days after intracameral transduction with BMP2. The data are representative of images taken of four mice.
    Anti Goat Immunoglobulin G, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Polysciences inc biotinylated anti goat igg ab
    Assessment of damage to peripheral retinal ganglion cells and optic nerves in the mouse eyes that overexpressed BMP2 in conventional outflow tissues. Thirty-six days after the viral infusion, the mouse eyes and the optic nerves were collected. A : An image from the periphery of the flatmounted retinas of transduced mouse eyes that were immunostained with Brn3a <t>immunoglobulin</t> G (IgG) and imaged with epifluorescence microscopy. B : The morphology of the myelinated axons (toluidine blue stained) of mice 36 days after intracameral transduction with BMP2. The data are representative of images taken of four mice.
    Biotinylated Anti Goat Igg Ab, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore goat antimouse igg
    Expression of human leucocyte antigen (HLA)-B27 (a) and monomorphic major histocompatibility complex (MHC) class I (b) molecules on monocytes from seven patients with Salmonella -triggered reactive arthritis (ReA) who were positive for the HLA-B27 gene during the acute phase of infection (1–4 weeks, patient 3 after 8 weeks) and during the follow-up. *Patients ◊ and ▪ did not recover during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (HLA-ABC-m3) and monomorphic MHC class I (w6/32) and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat <t>antimouse</t> <t>IgG.</t> The y -axis is the mean fluorescence intensity (MFI) of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting.
    Goat Antimouse Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    SouthernBiotech goat f
    Expression of human leucocyte antigen (HLA)-B27 (a) and monomorphic major histocompatibility complex (MHC) class I (b) molecules on monocytes from seven patients with Salmonella -triggered reactive arthritis (ReA) who were positive for the HLA-B27 gene during the acute phase of infection (1–4 weeks, patient 3 after 8 weeks) and during the follow-up. *Patients ◊ and ▪ did not recover during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (HLA-ABC-m3) and monomorphic MHC class I (w6/32) and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat <t>antimouse</t> <t>IgG.</t> The y -axis is the mean fluorescence intensity (MFI) of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting.
    Goat F, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 692 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) Co-immunoprecipitation was carried out using anti-BRG1 antibody (N-15). Goat IgG was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.

    Journal: BioResearch Open Access

    Article Title: BRG1 Is Required to Maintain Pluripotency of Murine Embryonic Stem Cells

    doi: 10.1089/biores.2013.0047

    Figure Lengend Snippet: Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) Co-immunoprecipitation was carried out using anti-BRG1 antibody (N-15). Goat IgG was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.

    Article Snippet: Nuclear extract (2500 μg) was precleared by incubation with 20 μL of anti-goat IgG antibody for 2 h. Immunoprecipitation was performed with 25 μL of anti-BRG1 (N-15) antibody (Santa Cruz sc-8749; Santa Cruz, CA) for 30 min at 4°C.

    Techniques: Immunoprecipitation, Cell Cycle Assay, BrdU Staining, Transfection, shRNA, Staining

    Immunization with pCVM-Tag induces a mixed IgG subtype distribution of antibodies to SV40 Tag.

    Journal: Journal of Virology

    Article Title: CD4+ T Lymphocytes Are Critical Mediators of Tumor Immunity to Simian Virus 40 Large Tumor Antigen Induced by Vaccination with Plasmid DNA ▿

    doi: 10.1128/JVI.00543-11

    Figure Lengend Snippet: Immunization with pCVM-Tag induces a mixed IgG subtype distribution of antibodies to SV40 Tag.

    Article Snippet: The ratio of IgG2a to IgG1 varied widely among individual mice, as two mice exhibited approximately equal quantities of the two subtypes (mouse 1 and mouse 2), two other mice exhibited higher quantities of IgG2a (mouse 4 and mouse 5), and one mouse exhibited higher quantities of IgG1 (mouse 3).

    Techniques:

    Assessment of damage to peripheral retinal ganglion cells and optic nerves in the mouse eyes that overexpressed BMP2 in conventional outflow tissues. Thirty-six days after the viral infusion, the mouse eyes and the optic nerves were collected. A : An image from the periphery of the flatmounted retinas of transduced mouse eyes that were immunostained with Brn3a immunoglobulin G (IgG) and imaged with epifluorescence microscopy. B : The morphology of the myelinated axons (toluidine blue stained) of mice 36 days after intracameral transduction with BMP2. The data are representative of images taken of four mice.

    Journal: Molecular Vision

    Article Title: Disease progression in iridocorneal angle tissues of BMP2-induced ocular hypertensive mice with optical coherence tomography

    doi:

    Figure Lengend Snippet: Assessment of damage to peripheral retinal ganglion cells and optic nerves in the mouse eyes that overexpressed BMP2 in conventional outflow tissues. Thirty-six days after the viral infusion, the mouse eyes and the optic nerves were collected. A : An image from the periphery of the flatmounted retinas of transduced mouse eyes that were immunostained with Brn3a immunoglobulin G (IgG) and imaged with epifluorescence microscopy. B : The morphology of the myelinated axons (toluidine blue stained) of mice 36 days after intracameral transduction with BMP2. The data are representative of images taken of four mice.

    Article Snippet: The eyes were then bisected, and the retinas were stained with goat anti-mouse Brn3a antibody (1:750 dilution, Santa Cruz Biotechnologies, Santa Cruz, CA) and anti-goat immunoglobulin G (IgG; H+L) Alexa Fluor 568 (1:500 dilution, Molecular Probes).

    Techniques: Epifluorescence Microscopy, Staining, Mouse Assay, Transduction

    Expression of human leucocyte antigen (HLA)-B27 (a) and monomorphic major histocompatibility complex (MHC) class I (b) molecules on monocytes from seven patients with Salmonella -triggered reactive arthritis (ReA) who were positive for the HLA-B27 gene during the acute phase of infection (1–4 weeks, patient 3 after 8 weeks) and during the follow-up. *Patients ◊ and ▪ did not recover during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (HLA-ABC-m3) and monomorphic MHC class I (w6/32) and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The y -axis is the mean fluorescence intensity (MFI) of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting.

    Journal: Immunology

    Article Title: Enterobacterial infection modulates major histocompatibility complex class I expression on mononuclear cells

    doi: 10.1046/j.1365-2567.1999.00803.x

    Figure Lengend Snippet: Expression of human leucocyte antigen (HLA)-B27 (a) and monomorphic major histocompatibility complex (MHC) class I (b) molecules on monocytes from seven patients with Salmonella -triggered reactive arthritis (ReA) who were positive for the HLA-B27 gene during the acute phase of infection (1–4 weeks, patient 3 after 8 weeks) and during the follow-up. *Patients ◊ and ▪ did not recover during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (HLA-ABC-m3) and monomorphic MHC class I (w6/32) and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The y -axis is the mean fluorescence intensity (MFI) of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting.

    Article Snippet: Cells were then washed twice and incubated with fluorescein isothiocyanate (FITC)-labelled F(ab′)2 fragments of goat antimouse IgG (1:200 v/v) at +4° for 15 min (Sigma Chemical Company, St Louis, MO).

    Techniques: Expressing, Infection, Incubation, Fluorescence, Negative Control

    (a) Expression of human leucocyte antigen (HLA)-B27 and major histocompatibility complex (MHC) class I molecules on monocytes from the patient with uncomplicated Salmonella infection. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 or with negative control mAbs, and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The expression of HLA-B27 detected with mAb HLA-ABC-m3 is shown as black histograms and corresponding negative controls as white histograms. The x -axis is the mean fluorescence intensity (MFI) on a log scale and the y -axis is the relative number of the cells. (b) Expression of monomorphic MHC class I on monocytes from patients with S. enteritidis infection without reactive arthritis (ReA). The cells were incubated with mAb recognizing monomorphic MHC class I and then with FITC-labelled F(ab′) 2 fragments of goat antimouse IgG. The y -axis is the MFI of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting. (c) Expression of HLA-ABC-m3 and FD705 epitopes of HLA-B27 on monocytes from a healthy control subject (A, B and C). The second sample (B) was taken 5 weeks and the third sample (C) 1 year after the first sample (A).

    Journal: Immunology

    Article Title: Enterobacterial infection modulates major histocompatibility complex class I expression on mononuclear cells

    doi: 10.1046/j.1365-2567.1999.00803.x

    Figure Lengend Snippet: (a) Expression of human leucocyte antigen (HLA)-B27 and major histocompatibility complex (MHC) class I molecules on monocytes from the patient with uncomplicated Salmonella infection. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 or with negative control mAbs, and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The expression of HLA-B27 detected with mAb HLA-ABC-m3 is shown as black histograms and corresponding negative controls as white histograms. The x -axis is the mean fluorescence intensity (MFI) on a log scale and the y -axis is the relative number of the cells. (b) Expression of monomorphic MHC class I on monocytes from patients with S. enteritidis infection without reactive arthritis (ReA). The cells were incubated with mAb recognizing monomorphic MHC class I and then with FITC-labelled F(ab′) 2 fragments of goat antimouse IgG. The y -axis is the MFI of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting. (c) Expression of HLA-ABC-m3 and FD705 epitopes of HLA-B27 on monocytes from a healthy control subject (A, B and C). The second sample (B) was taken 5 weeks and the third sample (C) 1 year after the first sample (A).

    Article Snippet: Cells were then washed twice and incubated with fluorescein isothiocyanate (FITC)-labelled F(ab′)2 fragments of goat antimouse IgG (1:200 v/v) at +4° for 15 min (Sigma Chemical Company, St Louis, MO).

    Techniques: Expressing, Infection, Incubation, Negative Control, Fluorescence

    (a) Expression of human leucocyte antigen (HLA)-B27, HLA-A2, HLA-B7 and major histocompatibility complex (MHC) class I molecules on monocytes from patient 5 (P5) with Salmonella -triggered reactive arthritis (ReA) at the start of the infection and during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (three mAbs against different epitopes of HLA-B27), HLA-A2, HLA-B7 and monomorphic MHC class I and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The expression of MHC class I epitopes recognized with mAbs is shown as black histograms and corresponding negative controls as white histograms. The x -axis is the mean fluorescence intensity (MFI) on a log scale, and the y -axis is the relative number of the cells. (b) Expression of HLA-B27 and monomorphic MHC class I epitopes on monocytes from a patient with Yersinia -triggered ReA. The cells were incubated with mAbs specific for HLA-B27 (four mAbs against different epitopes of HLA-B27) and monomorphic MHC class I and then with FITC-labelled F(ab′) 2 fragments of goat antimouse IgG.

    Journal: Immunology

    Article Title: Enterobacterial infection modulates major histocompatibility complex class I expression on mononuclear cells

    doi: 10.1046/j.1365-2567.1999.00803.x

    Figure Lengend Snippet: (a) Expression of human leucocyte antigen (HLA)-B27, HLA-A2, HLA-B7 and major histocompatibility complex (MHC) class I molecules on monocytes from patient 5 (P5) with Salmonella -triggered reactive arthritis (ReA) at the start of the infection and during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (three mAbs against different epitopes of HLA-B27), HLA-A2, HLA-B7 and monomorphic MHC class I and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The expression of MHC class I epitopes recognized with mAbs is shown as black histograms and corresponding negative controls as white histograms. The x -axis is the mean fluorescence intensity (MFI) on a log scale, and the y -axis is the relative number of the cells. (b) Expression of HLA-B27 and monomorphic MHC class I epitopes on monocytes from a patient with Yersinia -triggered ReA. The cells were incubated with mAbs specific for HLA-B27 (four mAbs against different epitopes of HLA-B27) and monomorphic MHC class I and then with FITC-labelled F(ab′) 2 fragments of goat antimouse IgG.

    Article Snippet: Cells were then washed twice and incubated with fluorescein isothiocyanate (FITC)-labelled F(ab′)2 fragments of goat antimouse IgG (1:200 v/v) at +4° for 15 min (Sigma Chemical Company, St Louis, MO).

    Techniques: Expressing, Infection, Incubation, Fluorescence