anti-goat Search Results


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  • 99
    Vector Laboratories anti goat secondary antibody
    Anti Goat Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 154 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher anti goat secondary antibodies
    Anti Goat Secondary Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    GE Healthcare anti goat antibodies
    Anti Goat Antibodies, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Upstate Biotechnology Inc anti goat abs
    Anti Goat Abs, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Agilent technologies anti goat antibody
    Anti Goat Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Santa Cruz Biotechnology goat antibodies
    Goat Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 528 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Jackson Immuno anti goat antibodies
    Anti Goat Antibodies, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Santa Cruz Biotechnology anti goat igg antibody
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Anti Goat Igg Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    GE Healthcare sheep anti goat abs
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Sheep Anti Goat Abs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bangalore Genei mouse anti goat abs
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Mouse Anti Goat Abs, supplied by Bangalore Genei, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno anti goat hrp abs
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Anti Goat Hrp Abs, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore cy3 anti goat ab
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Cy3 Anti Goat Ab, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Santa Cruz Biotechnology bovine anti goat ab
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Bovine Anti Goat Ab, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies goat igg ab
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Goat Igg Ab, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Jackson Immuno donkey anti goat secondary abs
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Donkey Anti Goat Secondary Abs, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    The Jackson Laboratory anti goat abs
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Anti Goat Abs, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    rdi research diagnostics donkey anti goat ab
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Donkey Anti Goat Ab, supplied by rdi research diagnostics, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher anti goat a647 secondary ab
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Anti Goat A647 Secondary Ab, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    R&D Systems pe anti goat ig ab
    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) <t>Co-immunoprecipitation</t> was carried out using anti-BRG1 antibody (N-15). Goat <t>IgG</t> was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.
    Pe Anti Goat Ig Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Agilent technologies hrp conjugated anti goat abs
    Murine anti–VLP-19 sera neutralize <t>RSV</t> and competes with palivizumab. ( A ) PRNT RSV neutralization assay was performed with heat-inactivated sera from VLP-19–immunized mice and palivizumab. ( B ) ELISA plates were coated with sF and incubated with a constant amount of palivizumab mixed with dilutions of either negative sera or anti–VLP-19 sera. Bound palivizumab was detected with <t>HRP-conjugated</t> anti-human Abs. Following washes, color was developed with tetramethylbenzidine followed by 0.1 N HCl. OD was read at 450 nm, and percentage of inhibition was calculated by comparison with a negative control. Data shown reflect 1 of 3 independently performed experiments.
    Hrp Conjugated Anti Goat Abs, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher rabbit anti goat igg abs
    CD82 and its cholesterol-binding differentially regulate cellular release of EVs. (a) Extracellular staining by filipin and <t>Alexa488-conjugated</t> CTxb in Du145 transfectants. Equal number of the cells were cultured on glass coverslips for 2 days, then fixed and labelled with filipin or Alexa488-conjugated CTxb. For filipin staining, intercellular regions were imaged. For CTxb staining, pericellular regions were imaged. Scale bar: 10 µm. (b) Distributions of Annexin V and Annexin A2 in Du145 transfectant cells. Alexa488-conjugated recombinant Annexin V was used for phosphatidylserine labelling, while Annexin-A2 Ab was used for Annexin-A2 staining. Scale bar: 10 μm. (c) Colocalization of Ezrin with GM1 or Annexin A2 in EVs. For Ezrin and GM1 co-staining, the cells were labelled with the Abs, Alexa488-conjugated CTxB and DAPI. For Ezrin and Annexin A2 co-staining, the cells were incubated sequentially with the primary Abs, Cy3-conjugated donkey anti-goat <t>IgG,</t> normal goat IgG and Alexa594-conjugated goat anti-mouse IgG. Images were obtained by confocal microscopy. Scale bar: 10 µm. (d) The cells were seeded in six-well plate at 50% confluence and cultured in DMEM containing 1% exosome-depleted FBS for 2 – 3 days. The culture supernatants were collected, spun at 2000 × g for 10 min to remove cell debris, and then analysed with NanoSight instrument for EV number and size. Data are presented as mean ± SD (n = 3 individual experiments). *: p
    Rabbit Anti Goat Igg Abs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Santa Cruz Biotechnology hrp coupled anti goat ab
    CD82 and its cholesterol-binding differentially regulate cellular release of EVs. (a) Extracellular staining by filipin and <t>Alexa488-conjugated</t> CTxb in Du145 transfectants. Equal number of the cells were cultured on glass coverslips for 2 days, then fixed and labelled with filipin or Alexa488-conjugated CTxb. For filipin staining, intercellular regions were imaged. For CTxb staining, pericellular regions were imaged. Scale bar: 10 µm. (b) Distributions of Annexin V and Annexin A2 in Du145 transfectant cells. Alexa488-conjugated recombinant Annexin V was used for phosphatidylserine labelling, while Annexin-A2 Ab was used for Annexin-A2 staining. Scale bar: 10 μm. (c) Colocalization of Ezrin with GM1 or Annexin A2 in EVs. For Ezrin and GM1 co-staining, the cells were labelled with the Abs, Alexa488-conjugated CTxB and DAPI. For Ezrin and Annexin A2 co-staining, the cells were incubated sequentially with the primary Abs, Cy3-conjugated donkey anti-goat <t>IgG,</t> normal goat IgG and Alexa594-conjugated goat anti-mouse IgG. Images were obtained by confocal microscopy. Scale bar: 10 µm. (d) The cells were seeded in six-well plate at 50% confluence and cultured in DMEM containing 1% exosome-depleted FBS for 2 – 3 days. The culture supernatants were collected, spun at 2000 × g for 10 min to remove cell debris, and then analysed with NanoSight instrument for EV number and size. Data are presented as mean ± SD (n = 3 individual experiments). *: p
    Hrp Coupled Anti Goat Ab, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) Co-immunoprecipitation was carried out using anti-BRG1 antibody (N-15). Goat IgG was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.

    Journal: BioResearch Open Access

    Article Title: BRG1 Is Required to Maintain Pluripotency of Murine Embryonic Stem Cells

    doi: 10.1089/biores.2013.0047

    Figure Lengend Snippet: Interaction of BRG1 with OCT4, and the role of BRG1 in cell cycle and apoptosis. (A) Co-immunoprecipitation was carried out using anti-BRG1 antibody (N-15). Goat IgG was used as an isotype-specific control. OCT4 was found to be immunoprecipitated with BRG1, revealing an interaction between the BAF complex and OCT4. Immunoprecipitation also showed that β-CATENIN interacts with the BRG1-containing complex. (B) Cell cycle analysis was performed at 48 h posttransfection after BRG1 knockdown by BrdU staining. ESD3 cells showed reduced cell number in the S phase of the cell cycle upon BRG1 knockdown compared with cells transfected with control shRNA. (C) Annexin V staining was performed to detect apoptotic cells at 72 h posttransfection. ShRNA-mediated knockdown of BRG1 in ESD3 cells showed increased apoptosis compared with cells transfected with control shRNA.

    Article Snippet: Nuclear extract (2500 μg) was precleared by incubation with 20 μL of anti-goat IgG antibody for 2 h. Immunoprecipitation was performed with 25 μL of anti-BRG1 (N-15) antibody (Santa Cruz sc-8749; Santa Cruz, CA) for 30 min at 4°C.

    Techniques: Immunoprecipitation, Cell Cycle Assay, BrdU Staining, Transfection, shRNA, Staining

    Murine anti–VLP-19 sera neutralize RSV and competes with palivizumab. ( A ) PRNT RSV neutralization assay was performed with heat-inactivated sera from VLP-19–immunized mice and palivizumab. ( B ) ELISA plates were coated with sF and incubated with a constant amount of palivizumab mixed with dilutions of either negative sera or anti–VLP-19 sera. Bound palivizumab was detected with HRP-conjugated anti-human Abs. Following washes, color was developed with tetramethylbenzidine followed by 0.1 N HCl. OD was read at 450 nm, and percentage of inhibition was calculated by comparison with a negative control. Data shown reflect 1 of 3 independently performed experiments.

    Journal: The Journal of Clinical Investigation

    Article Title: Palivizumab epitope–displaying virus-like particles protect rodents from RSV challenge

    doi: 10.1172/JCI78450

    Figure Lengend Snippet: Murine anti–VLP-19 sera neutralize RSV and competes with palivizumab. ( A ) PRNT RSV neutralization assay was performed with heat-inactivated sera from VLP-19–immunized mice and palivizumab. ( B ) ELISA plates were coated with sF and incubated with a constant amount of palivizumab mixed with dilutions of either negative sera or anti–VLP-19 sera. Bound palivizumab was detected with HRP-conjugated anti-human Abs. Following washes, color was developed with tetramethylbenzidine followed by 0.1 N HCl. OD was read at 450 nm, and percentage of inhibition was calculated by comparison with a negative control. Data shown reflect 1 of 3 independently performed experiments.

    Article Snippet: Overlay was aspirated, and cells were immunostained with goat RSV Abs (Chemicon 1128) followed by HRP-conjugated anti-goat Abs (Dako).

    Techniques: Plaque Reduction Neutralization Test, Neutralization, Mouse Assay, Enzyme-linked Immunosorbent Assay, Incubation, Inhibition, Negative Control

    CD82 and its cholesterol-binding differentially regulate cellular release of EVs. (a) Extracellular staining by filipin and Alexa488-conjugated CTxb in Du145 transfectants. Equal number of the cells were cultured on glass coverslips for 2 days, then fixed and labelled with filipin or Alexa488-conjugated CTxb. For filipin staining, intercellular regions were imaged. For CTxb staining, pericellular regions were imaged. Scale bar: 10 µm. (b) Distributions of Annexin V and Annexin A2 in Du145 transfectant cells. Alexa488-conjugated recombinant Annexin V was used for phosphatidylserine labelling, while Annexin-A2 Ab was used for Annexin-A2 staining. Scale bar: 10 μm. (c) Colocalization of Ezrin with GM1 or Annexin A2 in EVs. For Ezrin and GM1 co-staining, the cells were labelled with the Abs, Alexa488-conjugated CTxB and DAPI. For Ezrin and Annexin A2 co-staining, the cells were incubated sequentially with the primary Abs, Cy3-conjugated donkey anti-goat IgG, normal goat IgG and Alexa594-conjugated goat anti-mouse IgG. Images were obtained by confocal microscopy. Scale bar: 10 µm. (d) The cells were seeded in six-well plate at 50% confluence and cultured in DMEM containing 1% exosome-depleted FBS for 2 – 3 days. The culture supernatants were collected, spun at 2000 × g for 10 min to remove cell debris, and then analysed with NanoSight instrument for EV number and size. Data are presented as mean ± SD (n = 3 individual experiments). *: p

    Journal: Journal of Extracellular Vesicles

    Article Title: Tetraspanin CD82 interaction with cholesterol promotes extracellular vesicle–mediated release of ezrin to inhibit tumour cell movement

    doi: 10.1080/20013078.2019.1692417

    Figure Lengend Snippet: CD82 and its cholesterol-binding differentially regulate cellular release of EVs. (a) Extracellular staining by filipin and Alexa488-conjugated CTxb in Du145 transfectants. Equal number of the cells were cultured on glass coverslips for 2 days, then fixed and labelled with filipin or Alexa488-conjugated CTxb. For filipin staining, intercellular regions were imaged. For CTxb staining, pericellular regions were imaged. Scale bar: 10 µm. (b) Distributions of Annexin V and Annexin A2 in Du145 transfectant cells. Alexa488-conjugated recombinant Annexin V was used for phosphatidylserine labelling, while Annexin-A2 Ab was used for Annexin-A2 staining. Scale bar: 10 μm. (c) Colocalization of Ezrin with GM1 or Annexin A2 in EVs. For Ezrin and GM1 co-staining, the cells were labelled with the Abs, Alexa488-conjugated CTxB and DAPI. For Ezrin and Annexin A2 co-staining, the cells were incubated sequentially with the primary Abs, Cy3-conjugated donkey anti-goat IgG, normal goat IgG and Alexa594-conjugated goat anti-mouse IgG. Images were obtained by confocal microscopy. Scale bar: 10 µm. (d) The cells were seeded in six-well plate at 50% confluence and cultured in DMEM containing 1% exosome-depleted FBS for 2 – 3 days. The culture supernatants were collected, spun at 2000 × g for 10 min to remove cell debris, and then analysed with NanoSight instrument for EV number and size. Data are presented as mean ± SD (n = 3 individual experiments). *: p

    Article Snippet: Secondary Abs were Alexa488- or Alexa594-conjugated goat anti-mouse IgG, goat anti-rabbit IgG, and rabbit anti-goat IgG Abs (ThermoFisher, MA), Cy3-conjugated donkey anti-goat IgG Ab (Jackson ImmunoResearch Laboratories, PA), Fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG Ab (Sigma-Aldrich, MO), and Horseradish Peroxidase (HRP)-conjugated goat anti-mouse and anti-rabbit IgG Abs (Sigma-Aldrich).

    Techniques: Binding Assay, Staining, Cell Culture, Transfection, Recombinant, Incubation, Confocal Microscopy