Journal: Journal of Cellular and Molecular Medicine
Article Title: ENO1‐targeted superparamagnetic iron oxide nanoparticles for detecting pancreatic cancer by magnetic resonance imaging
Figure Lengend Snippet: Detection of pancreatic tumour by in vivo MRI of ENO1‐Dex‐g‐PCL/SPIO nanoparticles. A, MRI of ENO1‐Dex‐g‐PCL/SPIO nanoparticles in a pancreatic cancer xenograft model. B, Compared with PBS control group and SPIO group, the T2 signal intensity of tumour tissue significantly decreased, and the tumour gradually darkened over time, in which the peak of enhancement was at 24 h in ENO1‐SPIO group. C, IHC staining (40×) of the pancreatic tumour tissues 24 h after injection with ENO1‐SPIO nanoparticles. D, Prussian blue staining (40×) of the pancreatic tumour tissues 24 h after injection with ENO1‐SPIO or SPIO. More positive iron particles were found in ENO1‐SPIO group. Results were achieved from representative experiments in triplicate and were shown as mean ± standard deviation (SD). * P < .05, ** P < .01
Article Snippet: To determine the cellular location of ENO1 protein in CFPAC‐1 and MiaPaCa‐2 cells, PDAC cells were incubated with primary antibody, and anti‐ENO1 (Abcam) diluted in 1% bovine serum albumin (BSA) (1:200) overnight at 4°C.
Techniques: In Vivo, Immunohistochemistry, Injection, Staining, Standard Deviation