Journal: Journal of Cellular and Molecular Medicine

Article Title: ENO1‐targeted superparamagnetic iron oxide nanoparticles for detecting pancreatic cancer by magnetic resonance imaging

doi: 10.1111/jcmm.15237

Figure Lengend Snippet: ENO1 expression of pancreatic cancer cell lines. A, Total expression level of ENO1 protein in MiaPaCa‐2 and CFPAC‐1 cells detected by Western blotting. B, Representative images showed cell‐surface expression of ENO1, which was analysed by flow cytometry in MiaPaCa‐2 and CFPAC‐1 cells. C, Subcellular expression of ENO1 was detected by immunofluorescence analysis in MiaPaCa‐2 and CFPAC‐1 cells. Results were achieved from representative experiments in triplicate

Article Snippet: To determine the cellular location of ENO1 protein in CFPAC‐1 and MiaPaCa‐2 cells, PDAC cells were incubated with primary antibody, and anti‐ENO1 (Abcam) diluted in 1% bovine serum albumin (BSA) (1:200) overnight at 4°C.

Techniques: Expressing, Western Blot, Flow Cytometry, Immunofluorescence

Journal: Journal of Cellular and Molecular Medicine

Article Title: ENO1‐targeted superparamagnetic iron oxide nanoparticles for detecting pancreatic cancer by magnetic resonance imaging

doi: 10.1111/jcmm.15237

Figure Lengend Snippet: Characterization of ENO1‐Dex‐g‐PCL/SPIO nanoparticles. A and B, ENO1‐Dex‐g‐PCL/SPIO nanoparticles had an average total size of 30 nm. C, The nanoparticles were detected by Fourier transform infrared spectroscopy, and the characteristic C = O absorption peak demonstrated that ENO1 antibody was connected to the SPIO surface. D, X‐ray diffractometry indicated the sample was mainly consisted of Fe 3 O 4 with complete crystal structure. E, Prussian blue staining showed more blue‐stained particles incubated with ENO1‐SPIO in CFPAC‐1 cells compared with SPIO. F, The hysteresis curve demonstrated that the nanoparticles had an appropriate property of superparamagnetism, and significantly decreases of T2 and T2* relaxation time were detected at different iron concentrations. Results were achieved from representative experiments in triplicate

Article Snippet: To determine the cellular location of ENO1 protein in CFPAC‐1 and MiaPaCa‐2 cells, PDAC cells were incubated with primary antibody, and anti‐ENO1 (Abcam) diluted in 1% bovine serum albumin (BSA) (1:200) overnight at 4°C.

Techniques: Spectroscopy, Staining, Incubation

Journal: Journal of Cellular and Molecular Medicine

Article Title: ENO1‐targeted superparamagnetic iron oxide nanoparticles for detecting pancreatic cancer by magnetic resonance imaging

doi: 10.1111/jcmm.15237

Figure Lengend Snippet: In vitro MRI of ENO1‐Dex‐g‐PCL/SPIO nanoparticles. A, MRI of ENO1‐Dex‐g‐PCL/SPIO nanoparticles in cultured CFPAC‐1 and MiaPaCa‐2 cells. B, Compared with SPIO group, the T2 and T2* values of CFPAC‐1 cells were notably decreased, which were incubated with ENO1‐SPIO. C, The R2 and R2* values of CFPAC‐1 cells were notably increased in ENO1‐SPIO group compared with SPIO group. Results were achieved from representative experiments in triplicate and were shown as mean ± standard deviation (SD). * P < .05, ** P < .01

Article Snippet: To determine the cellular location of ENO1 protein in CFPAC‐1 and MiaPaCa‐2 cells, PDAC cells were incubated with primary antibody, and anti‐ENO1 (Abcam) diluted in 1% bovine serum albumin (BSA) (1:200) overnight at 4°C.

Techniques: In Vitro, Cell Culture, Incubation, Standard Deviation

Journal: Journal of Cellular and Molecular Medicine

Article Title: ENO1‐targeted superparamagnetic iron oxide nanoparticles for detecting pancreatic cancer by magnetic resonance imaging

doi: 10.1111/jcmm.15237

Figure Lengend Snippet: Detection of pancreatic tumour by in vivo MRI of ENO1‐Dex‐g‐PCL/SPIO nanoparticles. A, MRI of ENO1‐Dex‐g‐PCL/SPIO nanoparticles in a pancreatic cancer xenograft model. B, Compared with PBS control group and SPIO group, the T2 signal intensity of tumour tissue significantly decreased, and the tumour gradually darkened over time, in which the peak of enhancement was at 24 h in ENO1‐SPIO group. C, IHC staining (40×) of the pancreatic tumour tissues 24 h after injection with ENO1‐SPIO nanoparticles. D, Prussian blue staining (40×) of the pancreatic tumour tissues 24 h after injection with ENO1‐SPIO or SPIO. More positive iron particles were found in ENO1‐SPIO group. Results were achieved from representative experiments in triplicate and were shown as mean ± standard deviation (SD). * P < .05, ** P < .01

Article Snippet: To determine the cellular location of ENO1 protein in CFPAC‐1 and MiaPaCa‐2 cells, PDAC cells were incubated with primary antibody, and anti‐ENO1 (Abcam) diluted in 1% bovine serum albumin (BSA) (1:200) overnight at 4°C.

Techniques: In Vivo, Immunohistochemistry, Injection, Staining, Standard Deviation

Journal: PLoS ONE

Article Title: Prophylactic Injection of Recombinant Alpha-Enolase Reduces Arthritis Severity in the Collagen-Induced Arthritis Mice Model

doi: 10.1371/journal.pone.0136359

Figure Lengend Snippet: 100 μ g CII was injected to DBA/1 mice at day 0 in CFA, a boost was performed 21 days later in IFA. Recombinant enolase (ENO1) or control (BSA) was injected subcutaneously one day before the first immunization with CII. For ENO1, two doses were tested (10 μ g = ENO1 10 μ g (n = 10) and 100 μ g = ENO1 100 μ g (n = 10)). For control mice (n = 10), BSA (100 μ g) was injected in the same buffer as the one used for ENO1 buffer. Clinical status was evaluated by global score (A), articular score (B), weight variation (C) and ankle thickness (D). Bars show the mean ± SEM; * = p < 0.05; ** = p < 0.01 by 2way ANOVA and Bonferroni post-test.

Article Snippet: Then, we used mouse anti-IgG HRP-conjugated antibodies (LifeTechnologies) for 1 h. TMB (Sigma) was used to reveal the presence of anti-ENO1.

Techniques: Injection, Recombinant

Journal: PLoS ONE

Article Title: Prophylactic Injection of Recombinant Alpha-Enolase Reduces Arthritis Severity in the Collagen-Induced Arthritis Mice Model

doi: 10.1371/journal.pone.0136359

Figure Lengend Snippet: Serum levels of anti-ENO1 (A), anti-pEP1 (C) and anti-collagen II (B and D) antibodies in control or treated mice were measured by ELISA. AU is considered as Arbitrary Units. Bars represent the mean ± SEM; * = p < 0.05; ** = p < 0.01, by 2way ANOVA and Bonferroni post-test (A and B); * = p < 0.05, by Mann-Withney (C and D).

Article Snippet: Then, we used mouse anti-IgG HRP-conjugated antibodies (LifeTechnologies) for 1 h. TMB (Sigma) was used to reveal the presence of anti-ENO1.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Prophylactic Injection of Recombinant Alpha-Enolase Reduces Arthritis Severity in the Collagen-Induced Arthritis Mice Model

doi: 10.1371/journal.pone.0136359

Figure Lengend Snippet: Serum levels of IL-4 (A and C) and IL-6 (B and D) in ENO1 treated mice (A-B) and pEP1 treated mice (C-D), compared to control mice, were measured by Luminex. Bars represent the mean ± SEM. Statistical analysis used are 2way ANOVA (A and B) or Mann-Withney (C and D).

Article Snippet: Then, we used mouse anti-IgG HRP-conjugated antibodies (LifeTechnologies) for 1 h. TMB (Sigma) was used to reveal the presence of anti-ENO1.

Techniques: Luminex

Journal: PLoS ONE

Article Title: Prophylactic Injection of Recombinant Alpha-Enolase Reduces Arthritis Severity in the Collagen-Induced Arthritis Mice Model

doi: 10.1371/journal.pone.0136359

Figure Lengend Snippet: Histological analyses were performed on CIA mice receiving prophylactic injection of ENO1 (A) or pEP1 (B) and were compared to those carried out in controls. Both hind and anterior paws were dissected and fixed for 48h in 10% phosphate-buffered formaldehyde 80 days after CII immunization. Different scores were assessed: inflammation (Infl), synovitis (Syno), cartilage resorption (Cart Res) and bone erosion (Bone Ero). In C, anterior paws of control mice (left) and anterior paws of CIA mice who received ENO1 100 μ g in a prophylactic way (right) were represented. 1: Synovial tissue hypertrophy; 2: Newly formed cartilage; 3: Fibrin deposit; 4: Cartilage resorption. Histograms show mean ± SEM; * = p < 0.05; ** = p < 0.01 by t-tests.

Article Snippet: Then, we used mouse anti-IgG HRP-conjugated antibodies (LifeTechnologies) for 1 h. TMB (Sigma) was used to reveal the presence of anti-ENO1.

Techniques: Injection

Journal: PLoS ONE

Article Title: Prophylactic Injection of Recombinant Alpha-Enolase Reduces Arthritis Severity in the Collagen-Induced Arthritis Mice Model

doi: 10.1371/journal.pone.0136359

Figure Lengend Snippet: Correlation between clinical and histological scores for experiments based on prophylactic injection of ENO1. Correlations between histological findings and clinical data were studied using Spearman’s rank correlation coefficient. Data are represented as Rs and p value.

Article Snippet: Then, we used mouse anti-IgG HRP-conjugated antibodies (LifeTechnologies) for 1 h. TMB (Sigma) was used to reveal the presence of anti-ENO1.

Techniques: Injection