anti-dut-antibody-stj117799 Search Results


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  • 99
    Roche mouse anti gfp
    Mouse Anti Gfp, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti gfp/product/Roche
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti gfp - by Bioz Stars, 2021-03
    99/100 stars
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    99
    Millipore dapi
    Dapi, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dapi/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dapi - by Bioz Stars, 2021-03
    99/100 stars
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    99
    Roche mouse anti ha
    Mouse Anti Ha, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti ha/product/Roche
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti ha - by Bioz Stars, 2021-03
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    99
    Roche mouse monoclonal anti gfp
    Enzymatic activity of NleB1 a , In vitro assay for NleB1 <t>GlcNAc</t> modification of FADD using recombinant proteins and 1 mM UDP-GlcNAc. Representative immunoblot from at least three independent experiments b , Intact protein mass spectrometry of FADD incubated with GST-NleB1 and UDP-GlcNAc. c , High resolution CID spectrum of the peptide corresponding to FADD 115-125 . *Diagnostic fragment ions that carry the GlcNAc modification. d , Cleaved caspase-8 in FasL-treated HeLa cells expressing <t>GFP,</t> GFP-NleB1 or GFP-NleB1 AAA . UT, untransfected. Actin; loading control. Representative immunoblot from at least three independent experiments e , Cleaved caspase-8 in HeLa cells infected with derivatives of EPEC and treated with FasL. Representative immunoblot from at least three independent experiments f , Quantification of cleaved caspase-8 by immunofluorescence microscopy of HeLa cells infected with derivatives of EPEC and treated with FasL. Results are mean ± SEM percentage cells with cleaved caspase-8 from two independent experiments counting ~200 cells in triplicate. * P
    Mouse Monoclonal Anti Gfp, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti gfp/product/Roche
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti gfp - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    N/A
    This gene encodes an essential enzyme of nucleotide metabolism The encoded protein forms a ubiquitous homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate This reaction serves two cellular purposes
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    Enzymatic activity of NleB1 a , In vitro assay for NleB1 GlcNAc modification of FADD using recombinant proteins and 1 mM UDP-GlcNAc. Representative immunoblot from at least three independent experiments b , Intact protein mass spectrometry of FADD incubated with GST-NleB1 and UDP-GlcNAc. c , High resolution CID spectrum of the peptide corresponding to FADD 115-125 . *Diagnostic fragment ions that carry the GlcNAc modification. d , Cleaved caspase-8 in FasL-treated HeLa cells expressing GFP, GFP-NleB1 or GFP-NleB1 AAA . UT, untransfected. Actin; loading control. Representative immunoblot from at least three independent experiments e , Cleaved caspase-8 in HeLa cells infected with derivatives of EPEC and treated with FasL. Representative immunoblot from at least three independent experiments f , Quantification of cleaved caspase-8 by immunofluorescence microscopy of HeLa cells infected with derivatives of EPEC and treated with FasL. Results are mean ± SEM percentage cells with cleaved caspase-8 from two independent experiments counting ~200 cells in triplicate. * P

    Journal: Nature

    Article Title: A type III effector antagonises death receptor signalling during bacterial gut infection

    doi: 10.1038/nature12524

    Figure Lengend Snippet: Enzymatic activity of NleB1 a , In vitro assay for NleB1 GlcNAc modification of FADD using recombinant proteins and 1 mM UDP-GlcNAc. Representative immunoblot from at least three independent experiments b , Intact protein mass spectrometry of FADD incubated with GST-NleB1 and UDP-GlcNAc. c , High resolution CID spectrum of the peptide corresponding to FADD 115-125 . *Diagnostic fragment ions that carry the GlcNAc modification. d , Cleaved caspase-8 in FasL-treated HeLa cells expressing GFP, GFP-NleB1 or GFP-NleB1 AAA . UT, untransfected. Actin; loading control. Representative immunoblot from at least three independent experiments e , Cleaved caspase-8 in HeLa cells infected with derivatives of EPEC and treated with FasL. Representative immunoblot from at least three independent experiments f , Quantification of cleaved caspase-8 by immunofluorescence microscopy of HeLa cells infected with derivatives of EPEC and treated with FasL. Results are mean ± SEM percentage cells with cleaved caspase-8 from two independent experiments counting ~200 cells in triplicate. * P

    Article Snippet: Membranes were probed with the following primary antibodies where necessary: mouse monoclonal anti-O/N-GlcNAc (CTD110.6) (Cell Signalling), mouse monoclonal anti-FLAG M2-HRP (Sigma-Aldrich), mouse monoclonal anti-GFP (7.1 and 13.1) (Roche) or mouse monoclonal anti-β-actin (AC-15) diluted 1:1000 in TBS with 5% BSA and 0.1% Tween.

    Techniques: Activity Assay, In Vitro, Modification, Recombinant, Mass Spectrometry, Incubation, Diagnostic Assay, Expressing, Infection, Immunofluorescence, Microscopy

    NleB1 binds death domain proteins and inhibits caspase-8 activation a , Yeast two-hybrid analysis of protein-protein interactions in S. cerevisiae PJ69-4A. Results are mean ± SEM β-galactosidase activity from at least three independent experiments performed in triplicate. b , Growth of S. cerevisiae PJ69-4A on medium to select for protein-protein interactions (left panel) or plasmid maintenance (right panel). Representative images from at least three independent experiments c , Yeast two-hybrid analysis of protein interactions in S. cerevisiae PJ69-4A. Results are mean ± SEM β-galactosidase activity from at least three independent experiments performed in triplicate. d , GFP-Trap® of GFP-NleB1 and detection of FADD-FLAG, TRADD-FLAG and RIPK1-FLAG in HEK293T cells. Actin; loading control. Representative immunoblot from at least three independent experiments e , MTT reduction in HeLa cells expressing GFP, GFP-NleB1 or GFP-NleB2. UT, untransfected. Results are the mean ± SEM of absorbance at 540 nm from three independent experiments performed in triplicate. * P

    Journal: Nature

    Article Title: A type III effector antagonises death receptor signalling during bacterial gut infection

    doi: 10.1038/nature12524

    Figure Lengend Snippet: NleB1 binds death domain proteins and inhibits caspase-8 activation a , Yeast two-hybrid analysis of protein-protein interactions in S. cerevisiae PJ69-4A. Results are mean ± SEM β-galactosidase activity from at least three independent experiments performed in triplicate. b , Growth of S. cerevisiae PJ69-4A on medium to select for protein-protein interactions (left panel) or plasmid maintenance (right panel). Representative images from at least three independent experiments c , Yeast two-hybrid analysis of protein interactions in S. cerevisiae PJ69-4A. Results are mean ± SEM β-galactosidase activity from at least three independent experiments performed in triplicate. d , GFP-Trap® of GFP-NleB1 and detection of FADD-FLAG, TRADD-FLAG and RIPK1-FLAG in HEK293T cells. Actin; loading control. Representative immunoblot from at least three independent experiments e , MTT reduction in HeLa cells expressing GFP, GFP-NleB1 or GFP-NleB2. UT, untransfected. Results are the mean ± SEM of absorbance at 540 nm from three independent experiments performed in triplicate. * P

    Article Snippet: Membranes were probed with the following primary antibodies where necessary: mouse monoclonal anti-O/N-GlcNAc (CTD110.6) (Cell Signalling), mouse monoclonal anti-FLAG M2-HRP (Sigma-Aldrich), mouse monoclonal anti-GFP (7.1 and 13.1) (Roche) or mouse monoclonal anti-β-actin (AC-15) diluted 1:1000 in TBS with 5% BSA and 0.1% Tween.

    Techniques: Activation Assay, Activity Assay, Plasmid Preparation, MTT Assay, Expressing