anti-dog igg (whole molecule)-peroxidase Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Millipore antibody anti dog igg whole molecule peroxidase
    Immunoblotting experiments using the recombinant proteins and a canine serological panel. For immunoblotting experiments, the rLiHyp1 (36.6 kDa), rLiHyp6 (21.4 kDa), and rHRF (19.4 kDa) proteins (10 μg, each) were submitted to a 12% SDS-PAGE and blotted onto a nitrocellulose membrane, which were blocked with a <t>PBS-T</t> containing 5% BSA solution, and incubated with a pool of sera of asymptomatic and symptomatic VL dogs, or with a pool containing sera of non-infected dogs (1:200 and 1:100 diluted in PBS-T, respectively). Peroxidase conjugated anti-dog <t>IgG</t> (1:10,000) was used as a second antibody. The reactivities against the rLiHyp1, rLiHyp6 and rHRF proteins are shown. A low range protein ladder (Invitrogen TM , Life Technologies, USA) was used (Mr). The individual reactions of the rLiHyp1, rLiHyp6, and rHRF proteins with the pools of sera from non-infected or L . infantum -infected dogs (NI and CVL, respectively) are shown. Immunoblottings were derived from three independent experiments, and one representative preparation is showed in this study.
    Antibody Anti Dog Igg Whole Molecule Peroxidase, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody anti dog igg whole molecule peroxidase/product/Millipore
    Average 93 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    antibody anti dog igg whole molecule peroxidase - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    85
    Millipore hrp conjugated rabbit anti dog immunoglobulin g
    Immunoblotting experiments using the recombinant proteins and a canine serological panel. For immunoblotting experiments, the rLiHyp1 (36.6 kDa), rLiHyp6 (21.4 kDa), and rHRF (19.4 kDa) proteins (10 μg, each) were submitted to a 12% SDS-PAGE and blotted onto a nitrocellulose membrane, which were blocked with a <t>PBS-T</t> containing 5% BSA solution, and incubated with a pool of sera of asymptomatic and symptomatic VL dogs, or with a pool containing sera of non-infected dogs (1:200 and 1:100 diluted in PBS-T, respectively). Peroxidase conjugated anti-dog <t>IgG</t> (1:10,000) was used as a second antibody. The reactivities against the rLiHyp1, rLiHyp6 and rHRF proteins are shown. A low range protein ladder (Invitrogen TM , Life Technologies, USA) was used (Mr). The individual reactions of the rLiHyp1, rLiHyp6, and rHRF proteins with the pools of sera from non-infected or L . infantum -infected dogs (NI and CVL, respectively) are shown. Immunoblottings were derived from three independent experiments, and one representative preparation is showed in this study.
    Hrp Conjugated Rabbit Anti Dog Immunoglobulin G, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated rabbit anti dog immunoglobulin g/product/Millipore
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated rabbit anti dog immunoglobulin g - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    Immunoblotting experiments using the recombinant proteins and a canine serological panel. For immunoblotting experiments, the rLiHyp1 (36.6 kDa), rLiHyp6 (21.4 kDa), and rHRF (19.4 kDa) proteins (10 μg, each) were submitted to a 12% SDS-PAGE and blotted onto a nitrocellulose membrane, which were blocked with a PBS-T containing 5% BSA solution, and incubated with a pool of sera of asymptomatic and symptomatic VL dogs, or with a pool containing sera of non-infected dogs (1:200 and 1:100 diluted in PBS-T, respectively). Peroxidase conjugated anti-dog IgG (1:10,000) was used as a second antibody. The reactivities against the rLiHyp1, rLiHyp6 and rHRF proteins are shown. A low range protein ladder (Invitrogen TM , Life Technologies, USA) was used (Mr). The individual reactions of the rLiHyp1, rLiHyp6, and rHRF proteins with the pools of sera from non-infected or L . infantum -infected dogs (NI and CVL, respectively) are shown. Immunoblottings were derived from three independent experiments, and one representative preparation is showed in this study.

    Journal: PLoS ONE

    Article Title: Antigenicity, Immunogenicity and Protective Efficacy of Three Proteins Expressed in the Promastigote and Amastigote Stages of Leishmania infantum against Visceral Leishmaniasis

    doi: 10.1371/journal.pone.0137683

    Figure Lengend Snippet: Immunoblotting experiments using the recombinant proteins and a canine serological panel. For immunoblotting experiments, the rLiHyp1 (36.6 kDa), rLiHyp6 (21.4 kDa), and rHRF (19.4 kDa) proteins (10 μg, each) were submitted to a 12% SDS-PAGE and blotted onto a nitrocellulose membrane, which were blocked with a PBS-T containing 5% BSA solution, and incubated with a pool of sera of asymptomatic and symptomatic VL dogs, or with a pool containing sera of non-infected dogs (1:200 and 1:100 diluted in PBS-T, respectively). Peroxidase conjugated anti-dog IgG (1:10,000) was used as a second antibody. The reactivities against the rLiHyp1, rLiHyp6 and rHRF proteins are shown. A low range protein ladder (Invitrogen TM , Life Technologies, USA) was used (Mr). The individual reactions of the rLiHyp1, rLiHyp6, and rHRF proteins with the pools of sera from non-infected or L . infantum -infected dogs (NI and CVL, respectively) are shown. Immunoblottings were derived from three independent experiments, and one representative preparation is showed in this study.

    Article Snippet: Plates were subsequently washed four times in PBS-T, and incubated with anti-dog IgG horseradish-peroxidase conjugated antibody (1:10,000, diluted in PBS-T; catalog A6792, Sigma Aldrich, USA), for 1 h at 37°C.

    Techniques: Recombinant, SDS Page, Incubation, Infection, Derivative Assay