Article Title: Kruppel-like factor 7 overexpression suppresses hematopoietic stem and progenitor cell function
Figure Lengend Snippet: KLF7 overexpression suppresses hematopoietic stem and progenitors. Bone marrow stem and progenitor populations were analyzed by flow cytometry 6 weeks after transplantation with control or KLF7 lentivirally transduced cells. (A) Gating strategy used to identify HSCs (KLS CD34 − ), common myeloid progenitors (CMP, Lin − c-Kit + Sca-1 − CD16/32 lo CD34 + ), granulocyte-monocyte progenitors (GMPs, Lin − C-Kit + Sca-1 − CD16/32 hi CD34 + ), and megakaryocytic-erythroid progenitors (MEP, Lin − c-Kit + Sca-1 − CD16/32 lo CD34 − ; top panel) and common lymphoid progenitors (CLP, Lin − CD27 + IL7R + Flk-2 + Ly6D − ; bottom panel). Cells are first gated on live, donor, and lineage-negative populations. (B) Representative histograms showing GFP expression in the indicated progenitor cell population. (C) A summary of the percentages of donor cells that are GFP + for the indicated progenitor population is provided. Input refers the percentage of bone marrow cells that were GFP + at the time of transplantation. Data represent the mean ± SEM of 2 independent experiments. *** P
Article Snippet: For the analysis of hematopoietic progenitor populations, bone marrow cells were incubated with the following progenitor antibody panel: anti–mouse Ly6A/E (Sca-1) PerCP-Cy5.5 (clone D7), anti–mouse CD117 (c-Kit) APC–eFluor 780 (clone 2B8), anti–mouse CD34 PE (clone HM34, BioLegend), anti–mouse CD16/32 eFluor 450 (clone 93), anti–mouse CD45.1 PE-Cy7 (clone A20), anti–mouse/human CD45R (B220) biotin (clone RA3-6B2), anti–mouse CD3e biotin (clone 145-2C11), anti–mouse Ly-6G (Gr-1) biotin (clone RB6–8C5), anti–mouse Ter119 biotin (clone TER-119), and streptavidin eFluor 605NC.
Techniques: Over Expression, Flow Cytometry, Cytometry, Transplantation Assay, Expressing