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  • 96
    Thermo Fisher anti cd34 pe
    Anti Cd34 Pe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd34 pe/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd34 pe - by Bioz Stars, 2021-04
    96/100 stars
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    86
    Becton Dickinson anti cd34 pe
    AHR antagonism with SR1 has redundant effects with MSI2 OE, and AHR activation with MSI2 OE results in a loss of HSPCs a, Representative flow plots and summary of <t>CD34</t> expression with MSI2 OE and control transduced CD34 + CB cells grown for 10 days in medium containing SR1 or DMSO vehicle (n=3 experiments). b, Fold change in CD34 expression from results shown in a . c, Fold-increase in CYP1B1 and AHRR transcript levels with FICZ in transduced cultures (n=3 experiments). d, Transduced CD34 + CB cells grown for 3 days in medium supplemented with FICZ and the corresponding change in CD34 expression. Each coloured pair (DMSO and FICZ) represents a matched CB sample (n=3 experiments). e, Differences in culture CD34 levels from d . All data presented as mean ± SEM. * p
    Anti Cd34 Pe, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd34 pe/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd34 pe - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    94
    BioLegend anti mouse cd34 pe
    KLF7 overexpression suppresses hematopoietic stem and progenitors. Bone marrow stem and progenitor populations were analyzed by flow cytometry 6 weeks after transplantation with control or KLF7 lentivirally transduced cells. (A) Gating strategy used to identify HSCs (KLS <t>CD34</t> − ), common myeloid progenitors (CMP, Lin − c-Kit + Sca-1 − CD16/32 lo CD34 + ), granulocyte-monocyte progenitors (GMPs, Lin − C-Kit + Sca-1 − CD16/32 hi CD34 + ), and megakaryocytic-erythroid progenitors (MEP, Lin − c-Kit + Sca-1 − CD16/32 lo CD34 − ; top panel) and common lymphoid progenitors (CLP, Lin − CD27 + IL7R + Flk-2 + Ly6D − ; bottom panel). Cells are first gated on live, donor, and lineage-negative populations. (B) Representative histograms showing GFP expression in the indicated progenitor cell population. (C) A summary of the percentages of donor cells that are GFP + for the indicated progenitor population is provided. Input refers the percentage of bone marrow cells that were GFP + at the time of transplantation. Data represent the mean ± SEM of 2 independent experiments. *** P
    Anti Mouse Cd34 Pe, supplied by BioLegend, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse cd34 pe/product/BioLegend
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mouse cd34 pe - by Bioz Stars, 2021-04
    94/100 stars
      Buy from Supplier

    86
    miltenyi biotec anti cd34 pe
    Induction of differentiation into B lymphocytes by co-culture with MS-5. (A) Ten days after starting co-culture of cord blood (CB) and MS-5. The photo reveals a growth of CB on MS-5. Phenotype analysis of non-adherent floating cells harvested after gentle agitation. <t>CD34</t> + cells are circled with blue dotted lines. Blue arrows, red arrows, and green arrows indicate CD38 + cells, CD43 + cells, and CD45 + cells, respectively. (B) Phenotype analysis of mixed floating and adherent cells 4 and 6 weeks after initiating the co-culture of CB and MS-5. The cell population indicated by each arrow is the same as in (A). CD19 + cells are circled with red dotted lines. (C) Phenotype analysis of mixed floating and adherent cells 3 and 5 weeks after initiating the co-culture of BiPSC13-derived CD34 + cells and MS-5. CD34 + cells are circled with blue dotted lines. Red arrows and green arrows indicate CD43 + cells and CD45 + cells, respectively. (D) Phenotype analysis of mixed floating and adherent cells 3 and 4 weeks after initiating the co-culture of MIB2-6-derived CD34 + cells and MS-5. The cell populations indicated by blue dotted lines and arrows are the same as in (C). (E) Phenotype analysis of mixed floating and adherent cells 4 and 6 weeks after initiating the co-culture of G#28-AID-derived CD34 + cells and MS-5. CD34 + cells are circled with blue dotted lines.
    Anti Cd34 Pe, supplied by miltenyi biotec, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd34 pe/product/miltenyi biotec
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd34 pe - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    N/A
    The RAM34 antibody reacts with the CD34 glycoprotein on the surface of three independently derived mouse CD34 transfected cell lines RAM34 antibody also reacts with the mouse cell lines PA6
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    Image Search Results


    AHR antagonism with SR1 has redundant effects with MSI2 OE, and AHR activation with MSI2 OE results in a loss of HSPCs a, Representative flow plots and summary of CD34 expression with MSI2 OE and control transduced CD34 + CB cells grown for 10 days in medium containing SR1 or DMSO vehicle (n=3 experiments). b, Fold change in CD34 expression from results shown in a . c, Fold-increase in CYP1B1 and AHRR transcript levels with FICZ in transduced cultures (n=3 experiments). d, Transduced CD34 + CB cells grown for 3 days in medium supplemented with FICZ and the corresponding change in CD34 expression. Each coloured pair (DMSO and FICZ) represents a matched CB sample (n=3 experiments). e, Differences in culture CD34 levels from d . All data presented as mean ± SEM. * p

    Journal: Nature

    Article Title: Musashi-2 Attenuates AHR Signaling to Expand Human Hematopoietic Stem Cells

    doi: 10.1038/nature17665

    Figure Lengend Snippet: AHR antagonism with SR1 has redundant effects with MSI2 OE, and AHR activation with MSI2 OE results in a loss of HSPCs a, Representative flow plots and summary of CD34 expression with MSI2 OE and control transduced CD34 + CB cells grown for 10 days in medium containing SR1 or DMSO vehicle (n=3 experiments). b, Fold change in CD34 expression from results shown in a . c, Fold-increase in CYP1B1 and AHRR transcript levels with FICZ in transduced cultures (n=3 experiments). d, Transduced CD34 + CB cells grown for 3 days in medium supplemented with FICZ and the corresponding change in CD34 expression. Each coloured pair (DMSO and FICZ) represents a matched CB sample (n=3 experiments). e, Differences in culture CD34 levels from d . All data presented as mean ± SEM. * p

    Article Snippet: Intracellular flow cytometry Lin− CB cells were initially stained with anti-CD34 PE (581) and anit-CD38 APC (HB7) antibodies (BD Biosciences) then fixed with the Cytofix/Cytoperm kit (BD Biosciences) according to the manufacturer's instructions.

    Techniques: Activation Assay, Flow Cytometry, Expressing

    MSI2 OE expands LT-HSCs with ex vivo culture a, Transduced CD34 + CD133 + cells after one week of culture (n=4 experiments, unpaired t-test). b-d, CD45 + GFP + engraftment from mice receiving the highest two cell doses for D3 and D10 (n=8 mice for both conditions) and the highest three doses for D10 secondary mice (n=6 control and 9 MSI2 OE mice, Mann-Whitney test). e, Myelo-lymphopoiesis in D10 secondary mice. f, Multi-lineage LT-HSC frequency in BM cells of D10 primary mice. Dashed lines indicate 95% C.I. g, Numbers of GFP + HSCs as evaluated by LDA. h, Cumulative fold change in MSI2 OE-transduced HSCs. Data shown as mean ± SEM. *p

    Journal: Nature

    Article Title: Musashi-2 Attenuates AHR Signaling to Expand Human Hematopoietic Stem Cells

    doi: 10.1038/nature17665

    Figure Lengend Snippet: MSI2 OE expands LT-HSCs with ex vivo culture a, Transduced CD34 + CD133 + cells after one week of culture (n=4 experiments, unpaired t-test). b-d, CD45 + GFP + engraftment from mice receiving the highest two cell doses for D3 and D10 (n=8 mice for both conditions) and the highest three doses for D10 secondary mice (n=6 control and 9 MSI2 OE mice, Mann-Whitney test). e, Myelo-lymphopoiesis in D10 secondary mice. f, Multi-lineage LT-HSC frequency in BM cells of D10 primary mice. Dashed lines indicate 95% C.I. g, Numbers of GFP + HSCs as evaluated by LDA. h, Cumulative fold change in MSI2 OE-transduced HSCs. Data shown as mean ± SEM. *p

    Article Snippet: Intracellular flow cytometry Lin− CB cells were initially stained with anti-CD34 PE (581) and anit-CD38 APC (HB7) antibodies (BD Biosciences) then fixed with the Cytofix/Cytoperm kit (BD Biosciences) according to the manufacturer's instructions.

    Techniques: Ex Vivo, Mouse Assay, MANN-WHITNEY

    MSI2 OE represses CYP1B1 and HSP90 3'UTR Renilla Luciferase reporter activity a, CLIP-seq reads (replicate 1 in blue and replicate 2 in green) and clusters (purple) mapped to the 3'UTR of HSP90. Matches to the GUAG motif are shown in black. Mammal PhyloP score listed in last track. b and c, Representative data of mean per cell fluoresence for HSP90 and CYP1B1 protein in transduced CD34 + CB. Protein level in cells during in vitro culture was analyzed 3 days (D3) and 7 days (D7) after transduction and sorting for GFP. Corresponding secondary alone antibody staining is shown for each experiment. Each circle represents a cell, and greater than 200 cells were analyzed per condition. d and e, Levels of renilla luciferase activity in NIH-3T3 cells co-transfected with control or MSI2 OE vectors and the CYP1B1 or HSP90 wild type or TCC mutant 3'UTR luciferase reporter (dotted line indicates no change in renilla activity; n=4 CYP1B1 and n=3 HSP90 experiments). f, Flow plots of co-transduced CD34 + CB cells with MSI2 (GFP) and CYP1B1 (BFP) lentivirus. g, GFP + BFP + CFU-GEMMs generated from f were replated in to secondary CFU assays and enumerated for total number of colonies formed. A total of 24 CFU-GEMMs from MSI2-BFP and MSI2-CYP1B1 were replated (n=2 experiments). Data presented as mean ± SEM. ***p

    Journal: Nature

    Article Title: Musashi-2 Attenuates AHR Signaling to Expand Human Hematopoietic Stem Cells

    doi: 10.1038/nature17665

    Figure Lengend Snippet: MSI2 OE represses CYP1B1 and HSP90 3'UTR Renilla Luciferase reporter activity a, CLIP-seq reads (replicate 1 in blue and replicate 2 in green) and clusters (purple) mapped to the 3'UTR of HSP90. Matches to the GUAG motif are shown in black. Mammal PhyloP score listed in last track. b and c, Representative data of mean per cell fluoresence for HSP90 and CYP1B1 protein in transduced CD34 + CB. Protein level in cells during in vitro culture was analyzed 3 days (D3) and 7 days (D7) after transduction and sorting for GFP. Corresponding secondary alone antibody staining is shown for each experiment. Each circle represents a cell, and greater than 200 cells were analyzed per condition. d and e, Levels of renilla luciferase activity in NIH-3T3 cells co-transfected with control or MSI2 OE vectors and the CYP1B1 or HSP90 wild type or TCC mutant 3'UTR luciferase reporter (dotted line indicates no change in renilla activity; n=4 CYP1B1 and n=3 HSP90 experiments). f, Flow plots of co-transduced CD34 + CB cells with MSI2 (GFP) and CYP1B1 (BFP) lentivirus. g, GFP + BFP + CFU-GEMMs generated from f were replated in to secondary CFU assays and enumerated for total number of colonies formed. A total of 24 CFU-GEMMs from MSI2-BFP and MSI2-CYP1B1 were replated (n=2 experiments). Data presented as mean ± SEM. ***p

    Article Snippet: Intracellular flow cytometry Lin− CB cells were initially stained with anti-CD34 PE (581) and anit-CD38 APC (HB7) antibodies (BD Biosciences) then fixed with the Cytofix/Cytoperm kit (BD Biosciences) according to the manufacturer's instructions.

    Techniques: Luciferase, Activity Assay, Cross-linking Immunoprecipitation, In Vitro, Transduction, Staining, Transfection, Mutagenesis, Flow Cytometry, Generated

    MSI2 is highly expressed in human hematopoietic stem and progenitor cell populations a, Schematic of the human hematopoietic hierarchy showing key primitive cell populations and simplified surface marker expression. b, qRT-PCR analysis of MSI1 and MSI2 expression in Lin − CB cell populations (n=3 independent Lin- CB samples). c, Gating strategy used to sort sub-fractions of Lin − CB HSPCs for MSI2 qRT-PCR expression analysis (n=2 independent pooled Lin − CB samples). d, MSI2 expression across the human hematopoietic hierarchy. Each circle represents an independent gene expression dataset curated by HemaExplorer. e, Intracellular flow cytometry analysis of MSI2 protein levels in Lin − CB. Histograms show background staining with secondary antibody (red) and positive staining with anti-MSI2 antibody plus secondary in Lin − CB (blue). MSI2 fluorescence intensity was divided into quartiles of negative (Q1), low (Q2), mid (Q3) and high (Q4) level expression. f, Plots show cell percentage within each quartile from e that are CD34 + CD38 − (left) and CD34 + CD38 + (right) (n=2 independent Lin − CB samples). All data presented as mean ± SEM. *p

    Journal: Nature

    Article Title: Musashi-2 Attenuates AHR Signaling to Expand Human Hematopoietic Stem Cells

    doi: 10.1038/nature17665

    Figure Lengend Snippet: MSI2 is highly expressed in human hematopoietic stem and progenitor cell populations a, Schematic of the human hematopoietic hierarchy showing key primitive cell populations and simplified surface marker expression. b, qRT-PCR analysis of MSI1 and MSI2 expression in Lin − CB cell populations (n=3 independent Lin- CB samples). c, Gating strategy used to sort sub-fractions of Lin − CB HSPCs for MSI2 qRT-PCR expression analysis (n=2 independent pooled Lin − CB samples). d, MSI2 expression across the human hematopoietic hierarchy. Each circle represents an independent gene expression dataset curated by HemaExplorer. e, Intracellular flow cytometry analysis of MSI2 protein levels in Lin − CB. Histograms show background staining with secondary antibody (red) and positive staining with anti-MSI2 antibody plus secondary in Lin − CB (blue). MSI2 fluorescence intensity was divided into quartiles of negative (Q1), low (Q2), mid (Q3) and high (Q4) level expression. f, Plots show cell percentage within each quartile from e that are CD34 + CD38 − (left) and CD34 + CD38 + (right) (n=2 independent Lin − CB samples). All data presented as mean ± SEM. *p

    Article Snippet: Intracellular flow cytometry Lin− CB cells were initially stained with anti-CD34 PE (581) and anit-CD38 APC (HB7) antibodies (BD Biosciences) then fixed with the Cytofix/Cytoperm kit (BD Biosciences) according to the manufacturer's instructions.

    Techniques: Marker, Expressing, Quantitative RT-PCR, Flow Cytometry, Cytometry, Staining, Fluorescence

    MSI2 OE enhances in vitro CB progenitor activity and increases numbers of STRCs a, CFU output from transduced Lin − CB (n=9 control and 10 MSI2 OE cultures from 5 experiments). b, CFU-GEMM secondary CFU replating potential (n=24 control and 30 MSI2 OE from 2 experiments) and images of primary GEMMs (scale bar 200 μm). c, Number of secondary colonies per replated CFU-GEMM from b . d, CD34 expression in STRCs prior to transplant (n=3 experiments). e, Human chimerism at 3 weeks in mice transplanted with varying doses of transduced STRCs. Dashed line indicates engraftment cutoff (n=3 experiments). f, STRC frequency as determined by LDA from e . Dashed lines indicate 95% C.I. Data shown as mean ± SEM. *p

    Journal: Nature

    Article Title: Musashi-2 Attenuates AHR Signaling to Expand Human Hematopoietic Stem Cells

    doi: 10.1038/nature17665

    Figure Lengend Snippet: MSI2 OE enhances in vitro CB progenitor activity and increases numbers of STRCs a, CFU output from transduced Lin − CB (n=9 control and 10 MSI2 OE cultures from 5 experiments). b, CFU-GEMM secondary CFU replating potential (n=24 control and 30 MSI2 OE from 2 experiments) and images of primary GEMMs (scale bar 200 μm). c, Number of secondary colonies per replated CFU-GEMM from b . d, CD34 expression in STRCs prior to transplant (n=3 experiments). e, Human chimerism at 3 weeks in mice transplanted with varying doses of transduced STRCs. Dashed line indicates engraftment cutoff (n=3 experiments). f, STRC frequency as determined by LDA from e . Dashed lines indicate 95% C.I. Data shown as mean ± SEM. *p

    Article Snippet: Intracellular flow cytometry Lin− CB cells were initially stained with anti-CD34 PE (581) and anit-CD38 APC (HB7) antibodies (BD Biosciences) then fixed with the Cytofix/Cytoperm kit (BD Biosciences) according to the manufacturer's instructions.

    Techniques: In Vitro, Activity Assay, Expressing, Mouse Assay

    MSI2 OE enhances HSC activity after ex vivo culture a, Experimental layout to measure changes in HSC engraftment capacity and frequency with ex vivo culture. b, Representative flow plots of CD45 + GFP + reconstitution from mice receiving the highest cell dose transplanted per time point. c, Multilineage engraftment of mice injected with D3 cultures. d, Proportion of the human CD45 + graft containing GFP + cells from D3 primary mice relative to pre-transplant levels of GFP + cells before expansion. Shown are mice transplanted at the two highest doses (n=8 mice for both conditions). e, Proportion of the human CD45 + graft containing GFP + cells from D10 primary mice relative to pre-transplant levels of GFP + cells after expansion. Shown are mice transplanted at the two highest doses (n=8 mice for both conditions, one sample t-test, no change = 1). f, Summary of multilineage engraftment from mice in c . g, GFP mean fluoresence intensity (MFI) in D10 primary engrafted mice. Shown are mice transplanted with the highest three doses, n=11 control and 13 MSI2 mice. h, CD34 expression in GFP-high (top 60%) relative to GFP-low (bottom 40%) gated cells (set per mouse) from engrafted recipients in c . i, Number of transduced phenotyped HSCs after 7 days of culture from HSC expansion experiment described in a . Symbols represent individual mice and shaded symbols represent MSI2 OE. All data presented as mean ± SEM.

    Journal: Nature

    Article Title: Musashi-2 Attenuates AHR Signaling to Expand Human Hematopoietic Stem Cells

    doi: 10.1038/nature17665

    Figure Lengend Snippet: MSI2 OE enhances HSC activity after ex vivo culture a, Experimental layout to measure changes in HSC engraftment capacity and frequency with ex vivo culture. b, Representative flow plots of CD45 + GFP + reconstitution from mice receiving the highest cell dose transplanted per time point. c, Multilineage engraftment of mice injected with D3 cultures. d, Proportion of the human CD45 + graft containing GFP + cells from D3 primary mice relative to pre-transplant levels of GFP + cells before expansion. Shown are mice transplanted at the two highest doses (n=8 mice for both conditions). e, Proportion of the human CD45 + graft containing GFP + cells from D10 primary mice relative to pre-transplant levels of GFP + cells after expansion. Shown are mice transplanted at the two highest doses (n=8 mice for both conditions, one sample t-test, no change = 1). f, Summary of multilineage engraftment from mice in c . g, GFP mean fluoresence intensity (MFI) in D10 primary engrafted mice. Shown are mice transplanted with the highest three doses, n=11 control and 13 MSI2 mice. h, CD34 expression in GFP-high (top 60%) relative to GFP-low (bottom 40%) gated cells (set per mouse) from engrafted recipients in c . i, Number of transduced phenotyped HSCs after 7 days of culture from HSC expansion experiment described in a . Symbols represent individual mice and shaded symbols represent MSI2 OE. All data presented as mean ± SEM.

    Article Snippet: Intracellular flow cytometry Lin− CB cells were initially stained with anti-CD34 PE (581) and anit-CD38 APC (HB7) antibodies (BD Biosciences) then fixed with the Cytofix/Cytoperm kit (BD Biosciences) according to the manufacturer's instructions.

    Techniques: Activity Assay, Ex Vivo, Flow Cytometry, Mouse Assay, Injection, Expressing

    MSI2 KD impairs secondary CFU replating potential and HSC engraftment capacity a, Left: Schematic of MSI2- and control RFP-targeted shRNA lentiviruses. Right: Confirmation of MSI2 protein knockdown (both isoforms that can be detected by western blot) in transduced NB4 cells. b, CFU production by shMSI2 and shControl transduced Lin − CB (n=8 cultures from 4 experiments). c, Secondary CFU output from shMSI2 and images of representative secondary CFU (scale bar = 200 μm, performed on n=4 cultures from 2 experiments). d, Fold change in transduced cell number after 7 days in culture (n=4 experiments). e, Growth curves of cultures initiated with transduced Lin − CB cells (n=4 experiments). f, Experimental design to readout changes in HSC capacity with MSI2 KD. g, Left: Representative flow analysis of transduced CD34 + CD38 − derived human chimerism in NSG mouse BM. Right: Shown is the ratio of the percentage of GFP + cells in the CD45 + population post-transplant to the initial pre-transplant GFP + cell percentage. Dotted line indicates the proportion of GFP + cells is unchanged relative to input. (One sample t-test, no change = 1, n = 6 shControl and n = 8 shMSI2 mice pooled from 2 experiments). h, Representative flow plots and summary of multilineage engraftment with shControl and shMSI2 (gated on GFP + cells). Western blot source data is shown in Supplementary Figure 1 . Data presented as mean ± SEM. *p

    Journal: Nature

    Article Title: Musashi-2 Attenuates AHR Signaling to Expand Human Hematopoietic Stem Cells

    doi: 10.1038/nature17665

    Figure Lengend Snippet: MSI2 KD impairs secondary CFU replating potential and HSC engraftment capacity a, Left: Schematic of MSI2- and control RFP-targeted shRNA lentiviruses. Right: Confirmation of MSI2 protein knockdown (both isoforms that can be detected by western blot) in transduced NB4 cells. b, CFU production by shMSI2 and shControl transduced Lin − CB (n=8 cultures from 4 experiments). c, Secondary CFU output from shMSI2 and images of representative secondary CFU (scale bar = 200 μm, performed on n=4 cultures from 2 experiments). d, Fold change in transduced cell number after 7 days in culture (n=4 experiments). e, Growth curves of cultures initiated with transduced Lin − CB cells (n=4 experiments). f, Experimental design to readout changes in HSC capacity with MSI2 KD. g, Left: Representative flow analysis of transduced CD34 + CD38 − derived human chimerism in NSG mouse BM. Right: Shown is the ratio of the percentage of GFP + cells in the CD45 + population post-transplant to the initial pre-transplant GFP + cell percentage. Dotted line indicates the proportion of GFP + cells is unchanged relative to input. (One sample t-test, no change = 1, n = 6 shControl and n = 8 shMSI2 mice pooled from 2 experiments). h, Representative flow plots and summary of multilineage engraftment with shControl and shMSI2 (gated on GFP + cells). Western blot source data is shown in Supplementary Figure 1 . Data presented as mean ± SEM. *p

    Article Snippet: Intracellular flow cytometry Lin− CB cells were initially stained with anti-CD34 PE (581) and anit-CD38 APC (HB7) antibodies (BD Biosciences) then fixed with the Cytofix/Cytoperm kit (BD Biosciences) according to the manufacturer's instructions.

    Techniques: shRNA, Western Blot, Flow Cytometry, Derivative Assay, Mouse Assay

    KLF7 overexpression suppresses hematopoietic stem and progenitors. Bone marrow stem and progenitor populations were analyzed by flow cytometry 6 weeks after transplantation with control or KLF7 lentivirally transduced cells. (A) Gating strategy used to identify HSCs (KLS CD34 − ), common myeloid progenitors (CMP, Lin − c-Kit + Sca-1 − CD16/32 lo CD34 + ), granulocyte-monocyte progenitors (GMPs, Lin − C-Kit + Sca-1 − CD16/32 hi CD34 + ), and megakaryocytic-erythroid progenitors (MEP, Lin − c-Kit + Sca-1 − CD16/32 lo CD34 − ; top panel) and common lymphoid progenitors (CLP, Lin − CD27 + IL7R + Flk-2 + Ly6D − ; bottom panel). Cells are first gated on live, donor, and lineage-negative populations. (B) Representative histograms showing GFP expression in the indicated progenitor cell population. (C) A summary of the percentages of donor cells that are GFP + for the indicated progenitor population is provided. Input refers the percentage of bone marrow cells that were GFP + at the time of transplantation. Data represent the mean ± SEM of 2 independent experiments. *** P

    Journal: Blood

    Article Title: Kruppel-like factor 7 overexpression suppresses hematopoietic stem and progenitor cell function

    doi: 10.1182/blood-2012-02-409839

    Figure Lengend Snippet: KLF7 overexpression suppresses hematopoietic stem and progenitors. Bone marrow stem and progenitor populations were analyzed by flow cytometry 6 weeks after transplantation with control or KLF7 lentivirally transduced cells. (A) Gating strategy used to identify HSCs (KLS CD34 − ), common myeloid progenitors (CMP, Lin − c-Kit + Sca-1 − CD16/32 lo CD34 + ), granulocyte-monocyte progenitors (GMPs, Lin − C-Kit + Sca-1 − CD16/32 hi CD34 + ), and megakaryocytic-erythroid progenitors (MEP, Lin − c-Kit + Sca-1 − CD16/32 lo CD34 − ; top panel) and common lymphoid progenitors (CLP, Lin − CD27 + IL7R + Flk-2 + Ly6D − ; bottom panel). Cells are first gated on live, donor, and lineage-negative populations. (B) Representative histograms showing GFP expression in the indicated progenitor cell population. (C) A summary of the percentages of donor cells that are GFP + for the indicated progenitor population is provided. Input refers the percentage of bone marrow cells that were GFP + at the time of transplantation. Data represent the mean ± SEM of 2 independent experiments. *** P

    Article Snippet: For the analysis of hematopoietic progenitor populations, bone marrow cells were incubated with the following progenitor antibody panel: anti–mouse Ly6A/E (Sca-1) PerCP-Cy5.5 (clone D7), anti–mouse CD117 (c-Kit) APC–eFluor 780 (clone 2B8), anti–mouse CD34 PE (clone HM34, BioLegend), anti–mouse CD16/32 eFluor 450 (clone 93), anti–mouse CD45.1 PE-Cy7 (clone A20), anti–mouse/human CD45R (B220) biotin (clone RA3-6B2), anti–mouse CD3e biotin (clone 145-2C11), anti–mouse Ly-6G (Gr-1) biotin (clone RB6–8C5), anti–mouse Ter119 biotin (clone TER-119), and streptavidin eFluor 605NC.

    Techniques: Over Expression, Flow Cytometry, Cytometry, Transplantation Assay, Expressing

    Induction of differentiation into B lymphocytes by co-culture with MS-5. (A) Ten days after starting co-culture of cord blood (CB) and MS-5. The photo reveals a growth of CB on MS-5. Phenotype analysis of non-adherent floating cells harvested after gentle agitation. CD34 + cells are circled with blue dotted lines. Blue arrows, red arrows, and green arrows indicate CD38 + cells, CD43 + cells, and CD45 + cells, respectively. (B) Phenotype analysis of mixed floating and adherent cells 4 and 6 weeks after initiating the co-culture of CB and MS-5. The cell population indicated by each arrow is the same as in (A). CD19 + cells are circled with red dotted lines. (C) Phenotype analysis of mixed floating and adherent cells 3 and 5 weeks after initiating the co-culture of BiPSC13-derived CD34 + cells and MS-5. CD34 + cells are circled with blue dotted lines. Red arrows and green arrows indicate CD43 + cells and CD45 + cells, respectively. (D) Phenotype analysis of mixed floating and adherent cells 3 and 4 weeks after initiating the co-culture of MIB2-6-derived CD34 + cells and MS-5. The cell populations indicated by blue dotted lines and arrows are the same as in (C). (E) Phenotype analysis of mixed floating and adherent cells 4 and 6 weeks after initiating the co-culture of G#28-AID-derived CD34 + cells and MS-5. CD34 + cells are circled with blue dotted lines.

    Journal: Scientific Reports

    Article Title: Chromosomal translocation t(11;14) and p53 deletion induced by the CRISPR/Cas9 system in normal B cell-derived iPS cells

    doi: 10.1038/s41598-021-84628-5

    Figure Lengend Snippet: Induction of differentiation into B lymphocytes by co-culture with MS-5. (A) Ten days after starting co-culture of cord blood (CB) and MS-5. The photo reveals a growth of CB on MS-5. Phenotype analysis of non-adherent floating cells harvested after gentle agitation. CD34 + cells are circled with blue dotted lines. Blue arrows, red arrows, and green arrows indicate CD38 + cells, CD43 + cells, and CD45 + cells, respectively. (B) Phenotype analysis of mixed floating and adherent cells 4 and 6 weeks after initiating the co-culture of CB and MS-5. The cell population indicated by each arrow is the same as in (A). CD19 + cells are circled with red dotted lines. (C) Phenotype analysis of mixed floating and adherent cells 3 and 5 weeks after initiating the co-culture of BiPSC13-derived CD34 + cells and MS-5. CD34 + cells are circled with blue dotted lines. Red arrows and green arrows indicate CD43 + cells and CD45 + cells, respectively. (D) Phenotype analysis of mixed floating and adherent cells 3 and 4 weeks after initiating the co-culture of MIB2-6-derived CD34 + cells and MS-5. The cell populations indicated by blue dotted lines and arrows are the same as in (C). (E) Phenotype analysis of mixed floating and adherent cells 4 and 6 weeks after initiating the co-culture of G#28-AID-derived CD34 + cells and MS-5. CD34 + cells are circled with blue dotted lines.

    Article Snippet: Phenotype analysis Purified CD34+ cells were evaluated by two-color and three-color flow cytometry after staining in phosphate-buffered saline without calcium chloride or magnesium chloride with the following monoclonal antibodies: anti-CD19-PE, anti-CD34-PE (BioLegend, San Diego, CA, USA), anti-CD38-FITC, anti-CD43-FITC, anti-CD10-FITC (BioLegend), and anti-CD45-PE/Cy5 (BioLegend).

    Techniques: Co-Culture Assay, Derivative Assay