anti-cd31 Search Results


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  • 95
    Millipore anti cd 31 antibody
    Treatment of HF mice with the ERβ agonist DPN is associated with increased cardiac angiogenesis in HF mice. Confocal images of LV sections immunostained for <t>CD-31</t> ( a ), the overlay of CD-31 and WGA ( b ), and at higher display magnification of the white squares ( c ). d Quantification of capillary density as microvessels per cardiomyocyte. CTRL group was comprised of sham operated mice * P
    Anti Cd 31 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd 31 antibody/product/Millipore
    Average 95 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    anti cd 31 antibody - by Bioz Stars, 2020-08
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    94
    Abcam anti cd 31 staining
    Treatment of HF mice with the ERβ agonist DPN is associated with increased cardiac angiogenesis in HF mice. Confocal images of LV sections immunostained for <t>CD-31</t> ( a ), the overlay of CD-31 and WGA ( b ), and at higher display magnification of the white squares ( c ). d Quantification of capillary density as microvessels per cardiomyocyte. CTRL group was comprised of sham operated mice * P
    Anti Cd 31 Staining, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd 31 staining/product/Abcam
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    anti cd 31 staining - by Bioz Stars, 2020-08
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    94
    Abcam anti cd 31 antibody
    Rationale and applicability of the physiologic GC. ( A ) After 72 h of co-culture in the GC, a single layer of the glomerular endothelium (endothelium stained with CellTracker Red™, Thermo Scientific) closely apposed to a monolayer of podocytes (podocytes stained with CellTracker Green, Thermo Scientific), both of which were separated by a thin, extracellular membrane-coated membrane. ( B ) Immunostaining of endothelium <t>CD-31</t> (red) and podocyte (green) synaptopodin after 3 days of co-culture. ( C ) Representative phase-contrast images do not show different morphology between cells in the transwell and cells in the GC. All images are x400 magnification. ( D ) Quantification of apoptosis in the endothelium and podocyte show no differences in the microfluidic device and in the transwell. ( E ) GFB permeabilities obtained by measuring the rate of transport of FITC-inulin, FITC-BSA and FITC-IgG are significantly reduced in co-cultures compared with BME-coated only and monocultures of the endothelium or podocytes under a perfusion flow rate of 5 μL/min. Data are the mean ± SD from three separate experiments. *P
    Anti Cd 31 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd 31 antibody/product/Abcam
    Average 94 stars, based on 124 article reviews
    Price from $9.99 to $1999.99
    anti cd 31 antibody - by Bioz Stars, 2020-08
    94/100 stars
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    99
    Abcam anti cd 31
    The co-localization of prolyl oligopeptidase (POP) with <t>CD-31</t> in the Matrigel plug assay. CD-31 (green; fluorescein label) was shown to co-localize with POP (red; Texas Red label) in a double-label immunofluorescence of Matrigel plugs in POP group (A–C)
    Anti Cd 31, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd 31/product/Abcam
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    anti cd 31 - by Bioz Stars, 2020-08
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    99
    Abcam anticd31 primary antibody
    Effect of platelet CD40 on platelet-induced impairment of the endothelial recovery of wire-injured mouse carotid arteries. Immunostaining for the endothelial marker <t>CD31</t> was performed to determine the percentage of the luminal endothelial recovery after wire injury. A: Representative images of CD31 staining in both uninjured and injured carotid arteries in the indicated groups. CD40 −/− apoE −/− . Negative control staining with isotype-matched control antibody did not show detectable labeling. B: Quantitative analysis of the endothelial recovery of the wire-injured carotid arteries 7 days after injury. n = 6 per group ( B ). ∗ P
    Anticd31 Primary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anticd31 primary antibody/product/Abcam
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    anticd31 primary antibody - by Bioz Stars, 2020-08
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    95
    Abcam rat anti cd 31 antibody
    Post mortem tumor cryosection from prostate cancer (PC-3) xenografts. a Overview mosaic image of a whole PC-3 tumor tissue section co-stained with the peptide FnBPA5 Alexa-488 (red), together with polyclonal antibodies against Fn (green) and endothelial cell marker <t>CD-31</t> (PECAM-1) (blue). Scale bar, 1 mm b Zoomed-in high-resolution z-projection images of different indicated regions showing superpositions of the stains FnBPA5 (red), fibronectin (green), and CD-31 (blue), as well as the mature collagen fibers in the same regions as visualized by SHG (cyan). Analysis of neighboring pixels (indicated by the small box showing the 5 × 5 pixel matrix surrounding each analyzed pixel) between antibody-stained Fn, FnBPA5 peptide and collagen bundles (SHG) showed a clear spatial proximity of FnBPA5 with Fn (99.9% of all FnBPA5 pixels have at least one neighboring Fn pixel). A total of 87% of pixels from mature collagen bundles were found to be in close proximity (within the 5 × 5 matrix of neighboring pixels) of FnBPA5-positive pixels (19 images analyzed). Scale bar, 20 μm. c Representative z-projection images of PC-3 tumor tissue sections stained for α-SMA and FnBPA5 and merged with nuclei stainings using DAPI. Analysis of neighboring pixels (indicated by the small box showing the 5 × 5 pixel matrix surrounding each analyzed pixel) reveals that 94% of the α-SMA-positive pixels are in close proximity (within the 5 × 5 matrix of neighboring pixels) to FnBPA5-positive regions (60 images analyzed). Lower thresholds were set based on individual channels to exclude unspecific pixels from the analysis, and a 5 × 5 matrix was used for the spatial proximity analysis of each pixel in 60 images, respectively, with each of the analyzed pixels being in the center (Methods section for more details). Scale bar, 20 μm
    Rat Anti Cd 31 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat anti cd 31 antibody/product/Abcam
    Average 95 stars, based on 4 article reviews
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    94
    Boster Bio anti cd31
    Immunohistochemical staining of the sections of representative rat femoral heads. a Representative images of <t>CD31</t> staining in the control, MPS, and MPS + VO-OHpic groups. b Relative CD31 expression was measured with the ImageJ software. ∗ P
    Anti Cd31, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd31/product/Boster Bio
    Average 94 stars, based on 37 article reviews
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    99
    BioLegend anti cd31
    Copper lowering in vivo reduces tumor endothelia proliferation and ICAM (CD54) expression. Mice treated with PBS, TM, penicillamine or trientine were sacrificed when tumors reached 100 mm 2 and tumor endothelia analyzed by flow cytometry. Single cells were identified by excluding doublets by gating on FSC-A versus FSC-H plots; A. Isotype controls and single stains were used to set up negative and positive regions (not shown). Lymphocytes were excluded by gating. Backgating on <t>CD31</t> + CD105 + revealed the FSC/SSC region associated with tumor blood vessels (BV:B). CD31 + CD105 + angiogenic (C and D) or CD31 + CD34 + normal (E) endothelial cells were identified, gated and quantified. The proportions of Ki67 + , CD105 + and CD54 + cells within each gate were determined (F). Pooled data counting > 20,000 CD31 + CD105 + or CD31 + CD34 + cells per mouse from 2 mice/treatment group and 4 control mice is shown as mean ± SEM. * p
    Anti Cd31, supplied by BioLegend, used in various techniques. Bioz Stars score: 99/100, based on 442 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd31/product/BioLegend
    Average 99 stars, based on 442 article reviews
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    anti cd31 - by Bioz Stars, 2020-08
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    95
    Abcam anti cd31 antibody ep3095
    Copper lowering in vivo reduces tumor endothelia proliferation and ICAM (CD54) expression. Mice treated with PBS, TM, penicillamine or trientine were sacrificed when tumors reached 100 mm 2 and tumor endothelia analyzed by flow cytometry. Single cells were identified by excluding doublets by gating on FSC-A versus FSC-H plots; A. Isotype controls and single stains were used to set up negative and positive regions (not shown). Lymphocytes were excluded by gating. Backgating on <t>CD31</t> + CD105 + revealed the FSC/SSC region associated with tumor blood vessels (BV:B). CD31 + CD105 + angiogenic (C and D) or CD31 + CD34 + normal (E) endothelial cells were identified, gated and quantified. The proportions of Ki67 + , CD105 + and CD54 + cells within each gate were determined (F). Pooled data counting > 20,000 CD31 + CD105 + or CD31 + CD34 + cells per mouse from 2 mice/treatment group and 4 control mice is shown as mean ± SEM. * p
    Anti Cd31 Antibody Ep3095, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd31 antibody ep3095/product/Abcam
    Average 95 stars, based on 13 article reviews
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    anti cd31 antibody ep3095 - by Bioz Stars, 2020-08
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    99
    Abcam anti cd31 antibody p2b1
    Copper lowering in vivo reduces tumor endothelia proliferation and ICAM (CD54) expression. Mice treated with PBS, TM, penicillamine or trientine were sacrificed when tumors reached 100 mm 2 and tumor endothelia analyzed by flow cytometry. Single cells were identified by excluding doublets by gating on FSC-A versus FSC-H plots; A. Isotype controls and single stains were used to set up negative and positive regions (not shown). Lymphocytes were excluded by gating. Backgating on <t>CD31</t> + CD105 + revealed the FSC/SSC region associated with tumor blood vessels (BV:B). CD31 + CD105 + angiogenic (C and D) or CD31 + CD34 + normal (E) endothelial cells were identified, gated and quantified. The proportions of Ki67 + , CD105 + and CD54 + cells within each gate were determined (F). Pooled data counting > 20,000 CD31 + CD105 + or CD31 + CD34 + cells per mouse from 2 mice/treatment group and 4 control mice is shown as mean ± SEM. * p
    Anti Cd31 Antibody P2b1, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd31 antibody p2b1/product/Abcam
    Average 99 stars, based on 139 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Treatment of HF mice with the ERβ agonist DPN is associated with increased cardiac angiogenesis in HF mice. Confocal images of LV sections immunostained for CD-31 ( a ), the overlay of CD-31 and WGA ( b ), and at higher display magnification of the white squares ( c ). d Quantification of capillary density as microvessels per cardiomyocyte. CTRL group was comprised of sham operated mice * P

    Journal: Biology of Sex Differences

    Article Title: Estrogen rescues heart failure through estrogen receptor Beta activation

    doi: 10.1186/s13293-018-0206-6

    Figure Lengend Snippet: Treatment of HF mice with the ERβ agonist DPN is associated with increased cardiac angiogenesis in HF mice. Confocal images of LV sections immunostained for CD-31 ( a ), the overlay of CD-31 and WGA ( b ), and at higher display magnification of the white squares ( c ). d Quantification of capillary density as microvessels per cardiomyocyte. CTRL group was comprised of sham operated mice * P

    Article Snippet: For immunofluorescence staining, the heart cross sections (6 μm) were labeled with anti-CD-31 antibody (Millipore (04-1074), 1:200 dilution) and wheat germ agglutinin (WGA, Invitrogen, 1:500 dilution), and images were acquired with a confocal scanning microscope (Nikon).

    Techniques: Mouse Assay, Whole Genome Amplification

    uNK cells, but not uDC, could be involved in EEVSF during post-implantation period A. Images showing CCR7 + uDCs and CD31 + BVs in the uteri of DTR (Control) and CD11c:DTR mice at 6.5 dpc after DTx treatment at 5.5 dpc. Scale bars, 500 µm. UL, uterine lumen. B, C. Comparisons of CD31 + BVD (%) in the ULR, CTR and AMR, and numbers of different sized VSFs in the CTR at 6.5 dpc in Control and CD11c:DTR mice. Each group, n = 4. D. Images showing DBA-lectin + uNK cells and VEGFR3 + VSFs in the uteri at 8.5 treated with Fc and anti-NKG2D antibody. Scale bars, 500 µm. E, F. Comparisons of CD31 + BVD (%) in the ULR, CTR and AMR, and numbers of different sized VSFs in the CTR at 8.5 dpc treated with Fc and anti-mouse NKG2D antibody. Each group, n = 4. * p

    Journal: EMBO Molecular Medicine

    Article Title: VEGF-A regulated by progesterone governs uterine angiogenesis and vascular remodelling during pregnancy

    doi: 10.1002/emmm.201302618

    Figure Lengend Snippet: uNK cells, but not uDC, could be involved in EEVSF during post-implantation period A. Images showing CCR7 + uDCs and CD31 + BVs in the uteri of DTR (Control) and CD11c:DTR mice at 6.5 dpc after DTx treatment at 5.5 dpc. Scale bars, 500 µm. UL, uterine lumen. B, C. Comparisons of CD31 + BVD (%) in the ULR, CTR and AMR, and numbers of different sized VSFs in the CTR at 6.5 dpc in Control and CD11c:DTR mice. Each group, n = 4. D. Images showing DBA-lectin + uNK cells and VEGFR3 + VSFs in the uteri at 8.5 treated with Fc and anti-NKG2D antibody. Scale bars, 500 µm. E, F. Comparisons of CD31 + BVD (%) in the ULR, CTR and AMR, and numbers of different sized VSFs in the CTR at 8.5 dpc treated with Fc and anti-mouse NKG2D antibody. Each group, n = 4. * p

    Article Snippet: Samples were blocked with 5% donkey (or goat) serum in PBST (0.03% Triton X-100 in PBS) and incubated for 3 h at room temperature (RT) with the following primary antibodies and lectin: anti-CD105 (rat, clone MJ7/18, eBioscience), anti-CD31 (hamster, clone 2H8, Millipore, Billerica, MA, USA), anti-PH3 (rabbit polyclonal, Millipore), anti-PR (rabbit polyclonal, Abcam), anti-caspase-3 (rabbit polyclonal, R & D Systems), FITC-conjugated anti-α-SMA (mouse, clone 1A4, Sigma–Aldrich), anti-RFP (rabbit polyclonal, Abcam), anti-VEGFR3 (goat polyclonal, R & D Systems), anti-VEGF-A (goat polyclonal, R & D Systems), anti-VEGFR2 (rabbit monoclonal, TO14, gifted by Dr. Rolf A. Brekken), anti-Prox-1 (rabbit polyclonal, Relia Tech GmbH), anti-CCR7 (rabbit polyclonal, Abcam), anti-Tie2 (rabbit polyclonal, Santa Cruz Biotechnology), anti-angiopoietin-1 (rabbit polyclonal, Ab Frontier), anti-Ang2 monoclonal (Aprogen) or biotin-conjugated anti-DBA lectin (Sigma–Aldrich).

    Techniques: Mouse Assay

    Dynamic changes of vascular density, expressions of VEGF-A, PR and angiogenic growth factor receptors, and mural cell coverage onto blood vessels in the postpartum uteri A. Images showing CD31 + BVs in the endometrium at PD 0.5, 2.5 and 4.5. B, C. Comparisons of CD31 + BVD (%) and expressions of VEGF-A and PR in the endometrium of ENP, 6.5 dpc, and PD 0.5, 2.5 and 4.5. Each group, n = 4 * p

    Journal: EMBO Molecular Medicine

    Article Title: VEGF-A regulated by progesterone governs uterine angiogenesis and vascular remodelling during pregnancy

    doi: 10.1002/emmm.201302618

    Figure Lengend Snippet: Dynamic changes of vascular density, expressions of VEGF-A, PR and angiogenic growth factor receptors, and mural cell coverage onto blood vessels in the postpartum uteri A. Images showing CD31 + BVs in the endometrium at PD 0.5, 2.5 and 4.5. B, C. Comparisons of CD31 + BVD (%) and expressions of VEGF-A and PR in the endometrium of ENP, 6.5 dpc, and PD 0.5, 2.5 and 4.5. Each group, n = 4 * p

    Article Snippet: Samples were blocked with 5% donkey (or goat) serum in PBST (0.03% Triton X-100 in PBS) and incubated for 3 h at room temperature (RT) with the following primary antibodies and lectin: anti-CD105 (rat, clone MJ7/18, eBioscience), anti-CD31 (hamster, clone 2H8, Millipore, Billerica, MA, USA), anti-PH3 (rabbit polyclonal, Millipore), anti-PR (rabbit polyclonal, Abcam), anti-caspase-3 (rabbit polyclonal, R & D Systems), FITC-conjugated anti-α-SMA (mouse, clone 1A4, Sigma–Aldrich), anti-RFP (rabbit polyclonal, Abcam), anti-VEGFR3 (goat polyclonal, R & D Systems), anti-VEGF-A (goat polyclonal, R & D Systems), anti-VEGFR2 (rabbit monoclonal, TO14, gifted by Dr. Rolf A. Brekken), anti-Prox-1 (rabbit polyclonal, Relia Tech GmbH), anti-CCR7 (rabbit polyclonal, Abcam), anti-Tie2 (rabbit polyclonal, Santa Cruz Biotechnology), anti-angiopoietin-1 (rabbit polyclonal, Ab Frontier), anti-Ang2 monoclonal (Aprogen) or biotin-conjugated anti-DBA lectin (Sigma–Aldrich).

    Techniques:

    Distinct regional differences in uterine vascular features during early pregnancy A. Image showing CD31 + blood vessels (BVs) and GFP + embryo in the uterus at 8.5 dpc. Dotted-line divides the mid-sectioned uterus into the mesometrial region (MR) and anti-mesometrial region (AMR). The MR is subdivided into the central region (CTR) and uterine lumen region (ULR). VSF, vascular sinus folding. Scale bar, 500 µm. B–F. Images showing CD31 + uterine BVs. Sprouting (B, arrows), variable-sized VSF (C) and intussusceptive pillar (D, white arrowheads) are frequently present in the MR, whereas fine vascular network (E) and PH3 + proliferating ECs (F, yellow arrowheads) are present in the AMR at 6.5 dpc. Scale bars, 50 µm. G, H. Scanning electron micrographs showing vascular lumens (VL) without and with abundant intussusceptive pillars (arrowheads) in the MR at 6.5 dpc. Scale bars, 5 µm. I, J. Comparisons of numbers of vascular sprouts and intussusceptions in the uterine endometrium of the estrus stage of non-pregnancy (ENP), and the CTR and AMR at 6.5 dpc. Each group, n = 5–6. * p

    Journal: EMBO Molecular Medicine

    Article Title: VEGF-A regulated by progesterone governs uterine angiogenesis and vascular remodelling during pregnancy

    doi: 10.1002/emmm.201302618

    Figure Lengend Snippet: Distinct regional differences in uterine vascular features during early pregnancy A. Image showing CD31 + blood vessels (BVs) and GFP + embryo in the uterus at 8.5 dpc. Dotted-line divides the mid-sectioned uterus into the mesometrial region (MR) and anti-mesometrial region (AMR). The MR is subdivided into the central region (CTR) and uterine lumen region (ULR). VSF, vascular sinus folding. Scale bar, 500 µm. B–F. Images showing CD31 + uterine BVs. Sprouting (B, arrows), variable-sized VSF (C) and intussusceptive pillar (D, white arrowheads) are frequently present in the MR, whereas fine vascular network (E) and PH3 + proliferating ECs (F, yellow arrowheads) are present in the AMR at 6.5 dpc. Scale bars, 50 µm. G, H. Scanning electron micrographs showing vascular lumens (VL) without and with abundant intussusceptive pillars (arrowheads) in the MR at 6.5 dpc. Scale bars, 5 µm. I, J. Comparisons of numbers of vascular sprouts and intussusceptions in the uterine endometrium of the estrus stage of non-pregnancy (ENP), and the CTR and AMR at 6.5 dpc. Each group, n = 5–6. * p

    Article Snippet: Samples were blocked with 5% donkey (or goat) serum in PBST (0.03% Triton X-100 in PBS) and incubated for 3 h at room temperature (RT) with the following primary antibodies and lectin: anti-CD105 (rat, clone MJ7/18, eBioscience), anti-CD31 (hamster, clone 2H8, Millipore, Billerica, MA, USA), anti-PH3 (rabbit polyclonal, Millipore), anti-PR (rabbit polyclonal, Abcam), anti-caspase-3 (rabbit polyclonal, R & D Systems), FITC-conjugated anti-α-SMA (mouse, clone 1A4, Sigma–Aldrich), anti-RFP (rabbit polyclonal, Abcam), anti-VEGFR3 (goat polyclonal, R & D Systems), anti-VEGF-A (goat polyclonal, R & D Systems), anti-VEGFR2 (rabbit monoclonal, TO14, gifted by Dr. Rolf A. Brekken), anti-Prox-1 (rabbit polyclonal, Relia Tech GmbH), anti-CCR7 (rabbit polyclonal, Abcam), anti-Tie2 (rabbit polyclonal, Santa Cruz Biotechnology), anti-angiopoietin-1 (rabbit polyclonal, Ab Frontier), anti-Ang2 monoclonal (Aprogen) or biotin-conjugated anti-DBA lectin (Sigma–Aldrich).

    Techniques:

    Pharmacologic blockade of PR with RU486 negatively regulates VEGF-A expression in DSCs for decidual angiogenesis and EEVSF The pregnant mice were treated with control buffer (Control, C) and RU486 (8 mg/kg) at 5.5 dpc, and the uteri were harvested at 6.5 dpc and analysed. A. Comparison of VEGF-A expression in the uteri of VEGF +/LacZ mice. Each numbered region (square-dotted line) is magnified in right side. Scale bars, 500 µm. B. Semi-quantitative RT-PCR showing mRNA expressions of VEGF-A and VEGFR2 (VR2). Each line indicates gene transcript of VEGF-A 120 , VEGF-A 164 and VEGF-A 188 . C. FACS analysis showing CD31 + /CD45 − EC populations from the endometrium. Each group, n = 4. D. Images comparing CD31 + BVs in the ULR, CTR and AMR. Scale bars, 100 µm. E, F Comparisons of CD31 + BVD (%) in the ULR, CTR and AMR, and numbers of different sized VSFs in the CTR. Each group, n = 4. * p

    Journal: EMBO Molecular Medicine

    Article Title: VEGF-A regulated by progesterone governs uterine angiogenesis and vascular remodelling during pregnancy

    doi: 10.1002/emmm.201302618

    Figure Lengend Snippet: Pharmacologic blockade of PR with RU486 negatively regulates VEGF-A expression in DSCs for decidual angiogenesis and EEVSF The pregnant mice were treated with control buffer (Control, C) and RU486 (8 mg/kg) at 5.5 dpc, and the uteri were harvested at 6.5 dpc and analysed. A. Comparison of VEGF-A expression in the uteri of VEGF +/LacZ mice. Each numbered region (square-dotted line) is magnified in right side. Scale bars, 500 µm. B. Semi-quantitative RT-PCR showing mRNA expressions of VEGF-A and VEGFR2 (VR2). Each line indicates gene transcript of VEGF-A 120 , VEGF-A 164 and VEGF-A 188 . C. FACS analysis showing CD31 + /CD45 − EC populations from the endometrium. Each group, n = 4. D. Images comparing CD31 + BVs in the ULR, CTR and AMR. Scale bars, 100 µm. E, F Comparisons of CD31 + BVD (%) in the ULR, CTR and AMR, and numbers of different sized VSFs in the CTR. Each group, n = 4. * p

    Article Snippet: Samples were blocked with 5% donkey (or goat) serum in PBST (0.03% Triton X-100 in PBS) and incubated for 3 h at room temperature (RT) with the following primary antibodies and lectin: anti-CD105 (rat, clone MJ7/18, eBioscience), anti-CD31 (hamster, clone 2H8, Millipore, Billerica, MA, USA), anti-PH3 (rabbit polyclonal, Millipore), anti-PR (rabbit polyclonal, Abcam), anti-caspase-3 (rabbit polyclonal, R & D Systems), FITC-conjugated anti-α-SMA (mouse, clone 1A4, Sigma–Aldrich), anti-RFP (rabbit polyclonal, Abcam), anti-VEGFR3 (goat polyclonal, R & D Systems), anti-VEGF-A (goat polyclonal, R & D Systems), anti-VEGFR2 (rabbit monoclonal, TO14, gifted by Dr. Rolf A. Brekken), anti-Prox-1 (rabbit polyclonal, Relia Tech GmbH), anti-CCR7 (rabbit polyclonal, Abcam), anti-Tie2 (rabbit polyclonal, Santa Cruz Biotechnology), anti-angiopoietin-1 (rabbit polyclonal, Ab Frontier), anti-Ang2 monoclonal (Aprogen) or biotin-conjugated anti-DBA lectin (Sigma–Aldrich).

    Techniques: Expressing, Mouse Assay, Quantitative RT-PCR, FACS

    P4-PR axis positively regulates VEGF-A expression in DSCs for decidual angiogenesis and EEVSF A. Comparison of VEGF-A expression and PR expression in the uteri at 6.5 dpc. Each numbered region (square-dotted line) is magnified in right side. UL, uterine lumen; E, embryo. Scale bars, 500 µm. B. Comparisons of PR-A and PR-B protein levels in the uteri of EPN, 6.5 dpc and PD 2.5, and in mammary glands (Mg), ovary (Ov), brain (Br) and kidney (Ki) in 8-week-old non-pregnant female mouse. Loading of similar amount of protein for each sample is verified by a similar intensity of β-actin signal. C. Increase of VEGF-A mRNA expression in the primary cultured DSCs by P4 (10 µM) stimulation for 12 and 24 h. Data are presented as relative fold to the control (C) after normalization with GAPDH. Each group, n = 3. * p = 0.0232 versus C by unpaired t test. D. Images showing CD31 + BVs in the ULR, CTR and AMR of control PR-Cre-VEGF-A +/+ mice (PR Cre VA +/+ ) and PR-Cre-VEGF-A +/fl (PR Cre VA +/fl ) mice at 6.5 dpc. Scale bars, 100 µm. E, F. Comparisons of CD31 + BVD (%) in the ULR, CTR and AMR, and numbers of different sized VSFs in the CTR of control PR Cre VA +/+ and PR Cre VA +/fl mice at 6.5 dpc. Each group, n = 3. * p

    Journal: EMBO Molecular Medicine

    Article Title: VEGF-A regulated by progesterone governs uterine angiogenesis and vascular remodelling during pregnancy

    doi: 10.1002/emmm.201302618

    Figure Lengend Snippet: P4-PR axis positively regulates VEGF-A expression in DSCs for decidual angiogenesis and EEVSF A. Comparison of VEGF-A expression and PR expression in the uteri at 6.5 dpc. Each numbered region (square-dotted line) is magnified in right side. UL, uterine lumen; E, embryo. Scale bars, 500 µm. B. Comparisons of PR-A and PR-B protein levels in the uteri of EPN, 6.5 dpc and PD 2.5, and in mammary glands (Mg), ovary (Ov), brain (Br) and kidney (Ki) in 8-week-old non-pregnant female mouse. Loading of similar amount of protein for each sample is verified by a similar intensity of β-actin signal. C. Increase of VEGF-A mRNA expression in the primary cultured DSCs by P4 (10 µM) stimulation for 12 and 24 h. Data are presented as relative fold to the control (C) after normalization with GAPDH. Each group, n = 3. * p = 0.0232 versus C by unpaired t test. D. Images showing CD31 + BVs in the ULR, CTR and AMR of control PR-Cre-VEGF-A +/+ mice (PR Cre VA +/+ ) and PR-Cre-VEGF-A +/fl (PR Cre VA +/fl ) mice at 6.5 dpc. Scale bars, 100 µm. E, F. Comparisons of CD31 + BVD (%) in the ULR, CTR and AMR, and numbers of different sized VSFs in the CTR of control PR Cre VA +/+ and PR Cre VA +/fl mice at 6.5 dpc. Each group, n = 3. * p

    Article Snippet: Samples were blocked with 5% donkey (or goat) serum in PBST (0.03% Triton X-100 in PBS) and incubated for 3 h at room temperature (RT) with the following primary antibodies and lectin: anti-CD105 (rat, clone MJ7/18, eBioscience), anti-CD31 (hamster, clone 2H8, Millipore, Billerica, MA, USA), anti-PH3 (rabbit polyclonal, Millipore), anti-PR (rabbit polyclonal, Abcam), anti-caspase-3 (rabbit polyclonal, R & D Systems), FITC-conjugated anti-α-SMA (mouse, clone 1A4, Sigma–Aldrich), anti-RFP (rabbit polyclonal, Abcam), anti-VEGFR3 (goat polyclonal, R & D Systems), anti-VEGF-A (goat polyclonal, R & D Systems), anti-VEGFR2 (rabbit monoclonal, TO14, gifted by Dr. Rolf A. Brekken), anti-Prox-1 (rabbit polyclonal, Relia Tech GmbH), anti-CCR7 (rabbit polyclonal, Abcam), anti-Tie2 (rabbit polyclonal, Santa Cruz Biotechnology), anti-angiopoietin-1 (rabbit polyclonal, Ab Frontier), anti-Ang2 monoclonal (Aprogen) or biotin-conjugated anti-DBA lectin (Sigma–Aldrich).

    Techniques: Expressing, Cell Culture, Mouse Assay

    VEGF-A is highly expressed in the DSCs of pregnant uterus, but is not spatially matched with hypoxia and HIF-1α and HIF-2α expressions A–C. Images showing expressions of VEGF-A and VEGFR2 in the uteri of VEGF +/LacZ and VEGFR2 +/LacZ mice at ENP (A) and 6.5 dpc (B and C). Each numbered region (square-dotted line) is magnified and arrayed in below. The tissues were counterstained with hematoxylin (violet) following X-gal staining. UL, uterine lumen; E, embryo; PDZ, primary decidual zone; SDZ, secondary decidual zone; CTR, the central region; VSF, vascular sinus folding. Scale bars, 500 µm. D. Image showing Hypoxyprobe-1 + hypoxic region and CD31 + BVs in uterus at 6.5 dpc. Scale bar, 500 µm. E, F Expressions of HIF-1α and HIF-2α in the uteri at 6.5 dpc. Each numbered region (square-dotted line) is magnified and arrayed in right side. Scale bars, 500 µm.

    Journal: EMBO Molecular Medicine

    Article Title: VEGF-A regulated by progesterone governs uterine angiogenesis and vascular remodelling during pregnancy

    doi: 10.1002/emmm.201302618

    Figure Lengend Snippet: VEGF-A is highly expressed in the DSCs of pregnant uterus, but is not spatially matched with hypoxia and HIF-1α and HIF-2α expressions A–C. Images showing expressions of VEGF-A and VEGFR2 in the uteri of VEGF +/LacZ and VEGFR2 +/LacZ mice at ENP (A) and 6.5 dpc (B and C). Each numbered region (square-dotted line) is magnified and arrayed in below. The tissues were counterstained with hematoxylin (violet) following X-gal staining. UL, uterine lumen; E, embryo; PDZ, primary decidual zone; SDZ, secondary decidual zone; CTR, the central region; VSF, vascular sinus folding. Scale bars, 500 µm. D. Image showing Hypoxyprobe-1 + hypoxic region and CD31 + BVs in uterus at 6.5 dpc. Scale bar, 500 µm. E, F Expressions of HIF-1α and HIF-2α in the uteri at 6.5 dpc. Each numbered region (square-dotted line) is magnified and arrayed in right side. Scale bars, 500 µm.

    Article Snippet: Samples were blocked with 5% donkey (or goat) serum in PBST (0.03% Triton X-100 in PBS) and incubated for 3 h at room temperature (RT) with the following primary antibodies and lectin: anti-CD105 (rat, clone MJ7/18, eBioscience), anti-CD31 (hamster, clone 2H8, Millipore, Billerica, MA, USA), anti-PH3 (rabbit polyclonal, Millipore), anti-PR (rabbit polyclonal, Abcam), anti-caspase-3 (rabbit polyclonal, R & D Systems), FITC-conjugated anti-α-SMA (mouse, clone 1A4, Sigma–Aldrich), anti-RFP (rabbit polyclonal, Abcam), anti-VEGFR3 (goat polyclonal, R & D Systems), anti-VEGF-A (goat polyclonal, R & D Systems), anti-VEGFR2 (rabbit monoclonal, TO14, gifted by Dr. Rolf A. Brekken), anti-Prox-1 (rabbit polyclonal, Relia Tech GmbH), anti-CCR7 (rabbit polyclonal, Abcam), anti-Tie2 (rabbit polyclonal, Santa Cruz Biotechnology), anti-angiopoietin-1 (rabbit polyclonal, Ab Frontier), anti-Ang2 monoclonal (Aprogen) or biotin-conjugated anti-DBA lectin (Sigma–Aldrich).

    Techniques: Mouse Assay, Staining

    GPR124 protein expression in human brain vessels. a Immunofluorescence shows co-expression (merge, yellow) of GPR124 (red) and CD31 endothelial cell marker (green) in normal (top panel) and malformed human vessels (BAVM from HHT patient and human pilocytic astrocytoma). b GPR124 immunostaining in sporadic BAVM vessels (left panel) and control brain vessels (right panel) including superficial temporal artery (STA) and cerebral cortex vessels. GPR124 protein has apparent localization to the endothelium (black arrows) in both AVM and control brain vessels.

    Journal: Translational stroke research

    Article Title: G Protein-Coupled Receptor 124 (GPR124) Gene Polymorphisms and Risk of Brain Arteriovenous Malformation

    doi: 10.1007/s12975-012-0202-9

    Figure Lengend Snippet: GPR124 protein expression in human brain vessels. a Immunofluorescence shows co-expression (merge, yellow) of GPR124 (red) and CD31 endothelial cell marker (green) in normal (top panel) and malformed human vessels (BAVM from HHT patient and human pilocytic astrocytoma). b GPR124 immunostaining in sporadic BAVM vessels (left panel) and control brain vessels (right panel) including superficial temporal artery (STA) and cerebral cortex vessels. GPR124 protein has apparent localization to the endothelium (black arrows) in both AVM and control brain vessels.

    Article Snippet: Frozen sections (10 μm) were stained for GPR124 and CD31 expression as previously described [ ] using rabbit anti-GPR124 and hamster anti-CD31 (Millipore).

    Techniques: Expressing, Immunofluorescence, Marker, Immunostaining

    TN-C is predominantly expressed in stromal and endothelial cells and its ligand, integrin α9, is expressed on HSPCs. (A-B) Relative RT-PCR for TN-C mRNA expression on whole-BM (A) and on CD45 + , CD11b + , CD31 + CD45 − , PDGFRα + CD45 −

    Journal: Blood

    Article Title: Extracellular matrix protein tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration

    doi: 10.1182/blood-2011-11-393645

    Figure Lengend Snippet: TN-C is predominantly expressed in stromal and endothelial cells and its ligand, integrin α9, is expressed on HSPCs. (A-B) Relative RT-PCR for TN-C mRNA expression on whole-BM (A) and on CD45 + , CD11b + , CD31 + CD45 − , PDGFRα + CD45 −

    Article Snippet: The primary Abs used for immunohistochemistry (IHC) were hamster anti-CD31 Ab (2H8; Millipore), rat anti–CD31 Ab (MEC13.3; BD Biosciences), and anti–TN-C Ab (Abcam).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing

    Rationale and applicability of the physiologic GC. ( A ) After 72 h of co-culture in the GC, a single layer of the glomerular endothelium (endothelium stained with CellTracker Red™, Thermo Scientific) closely apposed to a monolayer of podocytes (podocytes stained with CellTracker Green, Thermo Scientific), both of which were separated by a thin, extracellular membrane-coated membrane. ( B ) Immunostaining of endothelium CD-31 (red) and podocyte (green) synaptopodin after 3 days of co-culture. ( C ) Representative phase-contrast images do not show different morphology between cells in the transwell and cells in the GC. All images are x400 magnification. ( D ) Quantification of apoptosis in the endothelium and podocyte show no differences in the microfluidic device and in the transwell. ( E ) GFB permeabilities obtained by measuring the rate of transport of FITC-inulin, FITC-BSA and FITC-IgG are significantly reduced in co-cultures compared with BME-coated only and monocultures of the endothelium or podocytes under a perfusion flow rate of 5 μL/min. Data are the mean ± SD from three separate experiments. *P

    Journal: Scientific Reports

    Article Title: Development of a Functional Glomerulus at the Organ Level on a Chip to Mimic Hypertensive Nephropathy

    doi: 10.1038/srep31771

    Figure Lengend Snippet: Rationale and applicability of the physiologic GC. ( A ) After 72 h of co-culture in the GC, a single layer of the glomerular endothelium (endothelium stained with CellTracker Red™, Thermo Scientific) closely apposed to a monolayer of podocytes (podocytes stained with CellTracker Green, Thermo Scientific), both of which were separated by a thin, extracellular membrane-coated membrane. ( B ) Immunostaining of endothelium CD-31 (red) and podocyte (green) synaptopodin after 3 days of co-culture. ( C ) Representative phase-contrast images do not show different morphology between cells in the transwell and cells in the GC. All images are x400 magnification. ( D ) Quantification of apoptosis in the endothelium and podocyte show no differences in the microfluidic device and in the transwell. ( E ) GFB permeabilities obtained by measuring the rate of transport of FITC-inulin, FITC-BSA and FITC-IgG are significantly reduced in co-cultures compared with BME-coated only and monocultures of the endothelium or podocytes under a perfusion flow rate of 5 μL/min. Data are the mean ± SD from three separate experiments. *P

    Article Snippet: Subsequently, sections were incubated with primary antibody: anti-CD-31 antibody (1:100; Abcam) and anti-nephrin antibody (1:100; Abcam) overnight.

    Techniques: Co-Culture Assay, Staining, Immunostaining, Flow Cytometry

    Hyper-perfusion damages the glomerular endothelium. ( A ) Immunostaining of endothelial cytoskeletal F-actin after exposure to a perfusion flow rate of 5, 10 and 15 μL/min, respectively, through the endothelium channel of the microdevice for 0, 6, 12 and 24 h. ( B ) Quantification of fluorescence intensity is also shown for F-actin. ( C ) Immunostaining of endothelial CD-31 in cell–cell junctions after exposure to a perfusion flow rate of 5, 10 and 15 μL/min, respectively, through the endothelium channel of the microdevice for 0, 6, 12 and 24 h. ( D ) Quantification of fluorescence intensity is also shown for CD-31. ( E ) Immunostaining of endothelial vWF after exposure to a perfusion flow rate of 5, 10 and 15 μL/min, respectively, through the endothelium channel of the microdevice for 0, 6, 12 and 24 h. ( F ) Quantification of fluorescent intensity is also shown for vWF. All images are at x400 magnification. Data are the mean ± SD from three separate experiments. *P

    Journal: Scientific Reports

    Article Title: Development of a Functional Glomerulus at the Organ Level on a Chip to Mimic Hypertensive Nephropathy

    doi: 10.1038/srep31771

    Figure Lengend Snippet: Hyper-perfusion damages the glomerular endothelium. ( A ) Immunostaining of endothelial cytoskeletal F-actin after exposure to a perfusion flow rate of 5, 10 and 15 μL/min, respectively, through the endothelium channel of the microdevice for 0, 6, 12 and 24 h. ( B ) Quantification of fluorescence intensity is also shown for F-actin. ( C ) Immunostaining of endothelial CD-31 in cell–cell junctions after exposure to a perfusion flow rate of 5, 10 and 15 μL/min, respectively, through the endothelium channel of the microdevice for 0, 6, 12 and 24 h. ( D ) Quantification of fluorescence intensity is also shown for CD-31. ( E ) Immunostaining of endothelial vWF after exposure to a perfusion flow rate of 5, 10 and 15 μL/min, respectively, through the endothelium channel of the microdevice for 0, 6, 12 and 24 h. ( F ) Quantification of fluorescent intensity is also shown for vWF. All images are at x400 magnification. Data are the mean ± SD from three separate experiments. *P

    Article Snippet: Subsequently, sections were incubated with primary antibody: anti-CD-31 antibody (1:100; Abcam) and anti-nephrin antibody (1:100; Abcam) overnight.

    Techniques: Immunostaining, Flow Cytometry, Fluorescence

    Pathologic changes in CD-31, vWF, nephrin and podocin in SHRs. ( A ) Immunohistochemistry showed reduced expression of the endothelial marker CD-31 in the SHR group, but not in the glomerulus of the control group. ( B ) Immunofluorescence showed increased expression of the marker of endothelial injury, vWF, in the SHR group, but not in the glomerulus of control group. ( C ) Immunohistochemistry showed reduced expression of the podocyte marker nephrin in the SHR group, but not in the glomerulus of the control group. ( D ) Immunofluorescence showed reduced expression of the podocyte marker podocin in the SHR group, but not in the glomerulus of the control group. All images are at x400 magnification.

    Journal: Scientific Reports

    Article Title: Development of a Functional Glomerulus at the Organ Level on a Chip to Mimic Hypertensive Nephropathy

    doi: 10.1038/srep31771

    Figure Lengend Snippet: Pathologic changes in CD-31, vWF, nephrin and podocin in SHRs. ( A ) Immunohistochemistry showed reduced expression of the endothelial marker CD-31 in the SHR group, but not in the glomerulus of the control group. ( B ) Immunofluorescence showed increased expression of the marker of endothelial injury, vWF, in the SHR group, but not in the glomerulus of control group. ( C ) Immunohistochemistry showed reduced expression of the podocyte marker nephrin in the SHR group, but not in the glomerulus of the control group. ( D ) Immunofluorescence showed reduced expression of the podocyte marker podocin in the SHR group, but not in the glomerulus of the control group. All images are at x400 magnification.

    Article Snippet: Subsequently, sections were incubated with primary antibody: anti-CD-31 antibody (1:100; Abcam) and anti-nephrin antibody (1:100; Abcam) overnight.

    Techniques: Immunohistochemistry, Expressing, Marker, Immunofluorescence

    MK0626 treatment increases vascularization and CXCL12/SDF-1 expression. (A) CD31 immunofluorescence of day 7 wounds in control vs glipizide-treated and MK0626-treated mice. (B) Bar graph of CD31 immunofluorescence from (A) (* P

    Journal: Translational research : the journal of laboratory and clinical medicine

    Article Title: Small molecule inhibition of dipeptidyl peptidase-4 enhances bone marrow progenitor cell function and angiogenesis in diabetic wounds

    doi: 10.1016/j.trsl.2018.10.006

    Figure Lengend Snippet: MK0626 treatment increases vascularization and CXCL12/SDF-1 expression. (A) CD31 immunofluorescence of day 7 wounds in control vs glipizide-treated and MK0626-treated mice. (B) Bar graph of CD31 immunofluorescence from (A) (* P

    Article Snippet: Sections were then incubated in CD31 antibody (ab28364; Abcam, Cambridge, Massachusetts) or SDF-1 antibody (ab25117; Abcam, Cambridge, Massachusetts) overnight, followed by a secondary antibody.

    Techniques: Expressing, Immunofluorescence, Mouse Assay

    Lymphangiogenesis and angiogenesis markers in omental and peritumoral adipose tissue Adipocyte diameter and immunohistochemical expression of podoplanin (A) and CD31 (B) were evaluated in hematoxylin and eosin stained sections. Representative images of specific staining of lymphatic (A) and blood (B) vessels in two consecutive tissue sections are reported in the upper part of panel. For both markers, positive cells per mm 2 were counted in omental and peritumoral adipose tissue depot of each patient. Non-parametric statistical tests were used. *p

    Journal: Oncotarget

    Article Title: Esophageal adenocarcinoma and obesity: peritumoral adipose tissue plays a role in lymph node invasion

    doi:

    Figure Lengend Snippet: Lymphangiogenesis and angiogenesis markers in omental and peritumoral adipose tissue Adipocyte diameter and immunohistochemical expression of podoplanin (A) and CD31 (B) were evaluated in hematoxylin and eosin stained sections. Representative images of specific staining of lymphatic (A) and blood (B) vessels in two consecutive tissue sections are reported in the upper part of panel. For both markers, positive cells per mm 2 were counted in omental and peritumoral adipose tissue depot of each patient. Non-parametric statistical tests were used. *p

    Article Snippet: Consecutive serial sections were then immunostained for Podoplanin (Anti-D240) and CD31 (Anti-CD31) (all from AbCam) and counterstained with hematoxylin.

    Techniques: Immunohistochemistry, Expressing, Staining

    Possible mechanism of crosstalk between peritumoral adipose tissue and tumor cells in EAC Obesity is associated with increased adipocyte size and altered secretion of several adipokines from adipose tissue. Peritumoral adipose tissue with increased cell diameter expresses higher levels of leptin, an adipokine that exerts a paracrine action on EAC cells by up-regulating the expression of EMT markers such as α-SMA and E-cadherin, and thus likely promotes the invasiveness of tumor. Moreover, increased adipocyte diameter in the peritumoral depot is also associated with increased levels of CD31 (an angiogenesis marker) and podoplanin (a lymphangiogenesis marker) and this enrichment of blood vessels and lymphatic vessels may represent a permissive condition that further supports the progression/invasion of tumor cells. In fact, leptin and podoplanin expression in peritumoral (but not distal) adipose tissue is strongly associated with nodal metastasis (N) in EAC patients. Figure created using Servier Medical Art ( http://www.servier.com/Powerpoint-image-bank ) by Servier, under CC BY 3.0.

    Journal: Oncotarget

    Article Title: Esophageal adenocarcinoma and obesity: peritumoral adipose tissue plays a role in lymph node invasion

    doi:

    Figure Lengend Snippet: Possible mechanism of crosstalk between peritumoral adipose tissue and tumor cells in EAC Obesity is associated with increased adipocyte size and altered secretion of several adipokines from adipose tissue. Peritumoral adipose tissue with increased cell diameter expresses higher levels of leptin, an adipokine that exerts a paracrine action on EAC cells by up-regulating the expression of EMT markers such as α-SMA and E-cadherin, and thus likely promotes the invasiveness of tumor. Moreover, increased adipocyte diameter in the peritumoral depot is also associated with increased levels of CD31 (an angiogenesis marker) and podoplanin (a lymphangiogenesis marker) and this enrichment of blood vessels and lymphatic vessels may represent a permissive condition that further supports the progression/invasion of tumor cells. In fact, leptin and podoplanin expression in peritumoral (but not distal) adipose tissue is strongly associated with nodal metastasis (N) in EAC patients. Figure created using Servier Medical Art ( http://www.servier.com/Powerpoint-image-bank ) by Servier, under CC BY 3.0.

    Article Snippet: Consecutive serial sections were then immunostained for Podoplanin (Anti-D240) and CD31 (Anti-CD31) (all from AbCam) and counterstained with hematoxylin.

    Techniques: Expressing, Marker

    VEGF-, TLR4L- and NLRL-induced cell infiltration and vessel formation in vivo Collagen gels individually containing medium alone (control), VEGF, TLR4L, or NOD1L were surgically implanted in the abdominal subcutaneous tissue of mice, harvested after 7 days, and stained for H E and CD31 to visualize vessel growth. Upper panels were taken at 10× and lower panels at 40× magnification. Figure is representative of 5 experiments (TLR4L-UP: ultrapure LPS; TLR4L-C: crude LPS).

    Journal: Gastroenterology

    Article Title: Pro-angiogenic activity of TLRs and NLRs: a novel link between gut microbiota and intestinal angiogenesis

    doi: 10.1053/j.gastro.2012.11.005

    Figure Lengend Snippet: VEGF-, TLR4L- and NLRL-induced cell infiltration and vessel formation in vivo Collagen gels individually containing medium alone (control), VEGF, TLR4L, or NOD1L were surgically implanted in the abdominal subcutaneous tissue of mice, harvested after 7 days, and stained for H E and CD31 to visualize vessel growth. Upper panels were taken at 10× and lower panels at 40× magnification. Figure is representative of 5 experiments (TLR4L-UP: ultrapure LPS; TLR4L-C: crude LPS).

    Article Snippet: Primary antibodies, (rabbit anti-CD31 and goat anti-GFP 1:100 dilution; Abcam 28365 and 6673, respectively; Abcam, Cambridage, MA) were applied in the same blocking buffer overnight at 4 C°.

    Techniques: In Vivo, Mouse Assay, Staining

    The co-localization of prolyl oligopeptidase (POP) with CD-31 in the Matrigel plug assay. CD-31 (green; fluorescein label) was shown to co-localize with POP (red; Texas Red label) in a double-label immunofluorescence of Matrigel plugs in POP group (A–C)

    Journal: British Journal of Pharmacology

    Article Title: Prolyl oligopeptidase induces angiogenesis both in vitro and in vivo in a novel regulatory manner

    doi: 10.1111/j.1476-5381.2010.01146.x

    Figure Lengend Snippet: The co-localization of prolyl oligopeptidase (POP) with CD-31 in the Matrigel plug assay. CD-31 (green; fluorescein label) was shown to co-localize with POP (red; Texas Red label) in a double-label immunofluorescence of Matrigel plugs in POP group (A–C)

    Article Snippet: Commercial rabbit anti-CD-31 antibody (Product# ab28364, AbCam, Cambridge, UK) has been tested for specificity using Western blot and used before in similar studies ( ).

    Techniques: Matrigel Assay, Immunofluorescence

    The effect of 4-phenyl-butanoyl-L-prolyl-2( S )-cyanopyrrolidine (KYP-2047) and human recombinant prolyl oligopeptidase (POP) protein on angiogenesis in vivo in Matrigel plug assay. Both doses of KYP-2047 inhibited angiogenesis measured by CD-31 immunoreactivity

    Journal: British Journal of Pharmacology

    Article Title: Prolyl oligopeptidase induces angiogenesis both in vitro and in vivo in a novel regulatory manner

    doi: 10.1111/j.1476-5381.2010.01146.x

    Figure Lengend Snippet: The effect of 4-phenyl-butanoyl-L-prolyl-2( S )-cyanopyrrolidine (KYP-2047) and human recombinant prolyl oligopeptidase (POP) protein on angiogenesis in vivo in Matrigel plug assay. Both doses of KYP-2047 inhibited angiogenesis measured by CD-31 immunoreactivity

    Article Snippet: Commercial rabbit anti-CD-31 antibody (Product# ab28364, AbCam, Cambridge, UK) has been tested for specificity using Western blot and used before in similar studies ( ).

    Techniques: Recombinant, In Vivo, Matrigel Assay

    Effect of platelet CD40 on platelet-induced impairment of the endothelial recovery of wire-injured mouse carotid arteries. Immunostaining for the endothelial marker CD31 was performed to determine the percentage of the luminal endothelial recovery after wire injury. A: Representative images of CD31 staining in both uninjured and injured carotid arteries in the indicated groups. CD40 −/− apoE −/− . Negative control staining with isotype-matched control antibody did not show detectable labeling. B: Quantitative analysis of the endothelial recovery of the wire-injured carotid arteries 7 days after injury. n = 6 per group ( B ). ∗ P

    Journal: The American Journal of Pathology

    Article Title: Platelet CD40 Mediates Leukocyte Recruitment and Neointima Formation after Arterial Denudation Injury in Atherosclerosis-Prone Mice

    doi: 10.1016/j.ajpath.2017.09.007

    Figure Lengend Snippet: Effect of platelet CD40 on platelet-induced impairment of the endothelial recovery of wire-injured mouse carotid arteries. Immunostaining for the endothelial marker CD31 was performed to determine the percentage of the luminal endothelial recovery after wire injury. A: Representative images of CD31 staining in both uninjured and injured carotid arteries in the indicated groups. CD40 −/− apoE −/− . Negative control staining with isotype-matched control antibody did not show detectable labeling. B: Quantitative analysis of the endothelial recovery of the wire-injured carotid arteries 7 days after injury. n = 6 per group ( B ). ∗ P

    Article Snippet: Paraffin-embedded cross sections (5 μm thick) of the common carotid arteries (four to five sections per mouse) were stained with the following antibodies: a goat polyclonal regulated on activation normal T cell expressed and secreted (RANTES)/chemokine (C-C motif) ligand (CCL) 5 antibody (C-19; 1:1000; Santa Cruz Biotechnology, Dallas, TX), monocyte chemoattractant protein (MCP)-1/CCL2 (1:1000; Santa Cruz Biotechnology), vascular cell adhesion molecule 1 (VCAM-1; 1:100; Abcam, Cambridge, UK), and anti–polymorphonuclear leukocyte (1:2500; Accurate, Austin, TX) to detect neutrophils; anti–Mac-2 (1:1000; M3/38; Accurate) to detect monocytes/macrophages; and anti-CD31 (1:50; Abcam) to detect endothelial cells.

    Techniques: Immunostaining, Marker, Staining, Negative Control, Labeling

    Post mortem tumor cryosection from prostate cancer (PC-3) xenografts. a Overview mosaic image of a whole PC-3 tumor tissue section co-stained with the peptide FnBPA5 Alexa-488 (red), together with polyclonal antibodies against Fn (green) and endothelial cell marker CD-31 (PECAM-1) (blue). Scale bar, 1 mm b Zoomed-in high-resolution z-projection images of different indicated regions showing superpositions of the stains FnBPA5 (red), fibronectin (green), and CD-31 (blue), as well as the mature collagen fibers in the same regions as visualized by SHG (cyan). Analysis of neighboring pixels (indicated by the small box showing the 5 × 5 pixel matrix surrounding each analyzed pixel) between antibody-stained Fn, FnBPA5 peptide and collagen bundles (SHG) showed a clear spatial proximity of FnBPA5 with Fn (99.9% of all FnBPA5 pixels have at least one neighboring Fn pixel). A total of 87% of pixels from mature collagen bundles were found to be in close proximity (within the 5 × 5 matrix of neighboring pixels) of FnBPA5-positive pixels (19 images analyzed). Scale bar, 20 μm. c Representative z-projection images of PC-3 tumor tissue sections stained for α-SMA and FnBPA5 and merged with nuclei stainings using DAPI. Analysis of neighboring pixels (indicated by the small box showing the 5 × 5 pixel matrix surrounding each analyzed pixel) reveals that 94% of the α-SMA-positive pixels are in close proximity (within the 5 × 5 matrix of neighboring pixels) to FnBPA5-positive regions (60 images analyzed). Lower thresholds were set based on individual channels to exclude unspecific pixels from the analysis, and a 5 × 5 matrix was used for the spatial proximity analysis of each pixel in 60 images, respectively, with each of the analyzed pixels being in the center (Methods section for more details). Scale bar, 20 μm

    Journal: Nature Communications

    Article Title: Novel peptide probes to assess the tensional state of fibronectin fibers in cancer

    doi: 10.1038/s41467-017-01846-0

    Figure Lengend Snippet: Post mortem tumor cryosection from prostate cancer (PC-3) xenografts. a Overview mosaic image of a whole PC-3 tumor tissue section co-stained with the peptide FnBPA5 Alexa-488 (red), together with polyclonal antibodies against Fn (green) and endothelial cell marker CD-31 (PECAM-1) (blue). Scale bar, 1 mm b Zoomed-in high-resolution z-projection images of different indicated regions showing superpositions of the stains FnBPA5 (red), fibronectin (green), and CD-31 (blue), as well as the mature collagen fibers in the same regions as visualized by SHG (cyan). Analysis of neighboring pixels (indicated by the small box showing the 5 × 5 pixel matrix surrounding each analyzed pixel) between antibody-stained Fn, FnBPA5 peptide and collagen bundles (SHG) showed a clear spatial proximity of FnBPA5 with Fn (99.9% of all FnBPA5 pixels have at least one neighboring Fn pixel). A total of 87% of pixels from mature collagen bundles were found to be in close proximity (within the 5 × 5 matrix of neighboring pixels) of FnBPA5-positive pixels (19 images analyzed). Scale bar, 20 μm. c Representative z-projection images of PC-3 tumor tissue sections stained for α-SMA and FnBPA5 and merged with nuclei stainings using DAPI. Analysis of neighboring pixels (indicated by the small box showing the 5 × 5 pixel matrix surrounding each analyzed pixel) reveals that 94% of the α-SMA-positive pixels are in close proximity (within the 5 × 5 matrix of neighboring pixels) to FnBPA5-positive regions (60 images analyzed). Lower thresholds were set based on individual channels to exclude unspecific pixels from the analysis, and a 5 × 5 matrix was used for the spatial proximity analysis of each pixel in 60 images, respectively, with each of the analyzed pixels being in the center (Methods section for more details). Scale bar, 20 μm

    Article Snippet: Samples were blocked in PBS with 5% goat serum for 60 min and incubated with polyclonal rabbit anti-fibronectin antibody (ab23750, Abcam, 1:50 dilution) or rat anti CD-31 antibody (ab7388, Abcam, 1:50 dilution) or rabbit anti-alpha SMA antibody (ab5694, Abcam, 1:100 dilution) overnight at 4°C.

    Techniques: Staining, Marker

    Immunohistochemical staining of the sections of representative rat femoral heads. a Representative images of CD31 staining in the control, MPS, and MPS + VO-OHpic groups. b Relative CD31 expression was measured with the ImageJ software. ∗ P

    Journal: Stem Cell Research & Therapy

    Article Title: PTEN inhibitor VO-OHpic attenuates GC-associated endothelial progenitor cell dysfunction and osteonecrosis of the femoral head via activating Nrf2 signaling and inhibiting mitochondrial apoptosis pathway

    doi: 10.1186/s13287-020-01658-y

    Figure Lengend Snippet: Immunohistochemical staining of the sections of representative rat femoral heads. a Representative images of CD31 staining in the control, MPS, and MPS + VO-OHpic groups. b Relative CD31 expression was measured with the ImageJ software. ∗ P

    Article Snippet: Some of these sections were stained with hematoxylin and eosin (H & E) to evaluate the trabecular structure, while the others were deparaffinized; antigen retrieved; incubated with anti-CD31, anti-VEGF, and anti-VEGFR2 primary antibodies; and then incubated with the corresponding biotinylated goat anti-rabbit (Boster, China, BA1003) and goat anti-mouse (Boster, China, BA1001) secondary antibodies.

    Techniques: Immunohistochemistry, Staining, Expressing, Software

    Copper lowering in vivo reduces tumor endothelia proliferation and ICAM (CD54) expression. Mice treated with PBS, TM, penicillamine or trientine were sacrificed when tumors reached 100 mm 2 and tumor endothelia analyzed by flow cytometry. Single cells were identified by excluding doublets by gating on FSC-A versus FSC-H plots; A. Isotype controls and single stains were used to set up negative and positive regions (not shown). Lymphocytes were excluded by gating. Backgating on CD31 + CD105 + revealed the FSC/SSC region associated with tumor blood vessels (BV:B). CD31 + CD105 + angiogenic (C and D) or CD31 + CD34 + normal (E) endothelial cells were identified, gated and quantified. The proportions of Ki67 + , CD105 + and CD54 + cells within each gate were determined (F). Pooled data counting > 20,000 CD31 + CD105 + or CD31 + CD34 + cells per mouse from 2 mice/treatment group and 4 control mice is shown as mean ± SEM. * p

    Journal: PLoS ONE

    Article Title: Rapid Copper Acquisition by Developing Murine Mesothelioma: Decreasing Bioavailable Copper Slows Tumor Growth, Normalizes Vessels and Promotes T Cell Infiltration

    doi: 10.1371/journal.pone.0073684

    Figure Lengend Snippet: Copper lowering in vivo reduces tumor endothelia proliferation and ICAM (CD54) expression. Mice treated with PBS, TM, penicillamine or trientine were sacrificed when tumors reached 100 mm 2 and tumor endothelia analyzed by flow cytometry. Single cells were identified by excluding doublets by gating on FSC-A versus FSC-H plots; A. Isotype controls and single stains were used to set up negative and positive regions (not shown). Lymphocytes were excluded by gating. Backgating on CD31 + CD105 + revealed the FSC/SSC region associated with tumor blood vessels (BV:B). CD31 + CD105 + angiogenic (C and D) or CD31 + CD34 + normal (E) endothelial cells were identified, gated and quantified. The proportions of Ki67 + , CD105 + and CD54 + cells within each gate were determined (F). Pooled data counting > 20,000 CD31 + CD105 + or CD31 + CD34 + cells per mouse from 2 mice/treatment group and 4 control mice is shown as mean ± SEM. * p

    Article Snippet: Markers for mouse endothelial cells were anti-CD31 (PECAM; Biolegend, San Diego, CA, USA); Alexa Fluor® 647-conjugated-anti-CD34 (found on normal endothelial cells and stem cells [ ]), PE-anti-CD105 (Endoglin, a regulatory component of TGF-β receptor specific for tumor angiogenesis [ ]), Alexa Fluor® 647-anti-CD106 (vascular cell adhesion molecule; VCAM) expressed on activated endothelial cells; all from eBioscience, San Diego, CA, USA.

    Techniques: In Vivo, Expressing, Mouse Assay, Flow Cytometry, Cytometry

    Decreasing bioavailable copper in vivo modulates tumor vessel dimensions. HUVECs (5 x 10 3 ) were treated in vitro with PBS (control is shown as the black line) or log fold concentrations of tetrathiomolybdate (A), penicillamine (B) and trientine (C) and MTT assays performed 24 hrs later. Data from n = 2 experiments each with its own triplicates is shown as mean ± range. Mice treated with PBS, TM, penicillamine or trientine were sacrificed when tumors reached 100 mm 2 and tumor-associated vessels visualized and measured on frozen tumor sections using FITC-anti-CD31 antibody and confocal imaging. Representative photographs are from individual mice that were not treated (control: D), given TM (E) or penicillamine (F). Pooled data counting a minimum of 100 cells per mouse showing tumor blood vessel (BV) diameter (G) and length (H) from control (normal mice; n = 4), penicillamine (Pen; n = 2), trientine (TT; n = 2) or Tm-treated (n = 2) mice are shown as mean ± SEM. * p

    Journal: PLoS ONE

    Article Title: Rapid Copper Acquisition by Developing Murine Mesothelioma: Decreasing Bioavailable Copper Slows Tumor Growth, Normalizes Vessels and Promotes T Cell Infiltration

    doi: 10.1371/journal.pone.0073684

    Figure Lengend Snippet: Decreasing bioavailable copper in vivo modulates tumor vessel dimensions. HUVECs (5 x 10 3 ) were treated in vitro with PBS (control is shown as the black line) or log fold concentrations of tetrathiomolybdate (A), penicillamine (B) and trientine (C) and MTT assays performed 24 hrs later. Data from n = 2 experiments each with its own triplicates is shown as mean ± range. Mice treated with PBS, TM, penicillamine or trientine were sacrificed when tumors reached 100 mm 2 and tumor-associated vessels visualized and measured on frozen tumor sections using FITC-anti-CD31 antibody and confocal imaging. Representative photographs are from individual mice that were not treated (control: D), given TM (E) or penicillamine (F). Pooled data counting a minimum of 100 cells per mouse showing tumor blood vessel (BV) diameter (G) and length (H) from control (normal mice; n = 4), penicillamine (Pen; n = 2), trientine (TT; n = 2) or Tm-treated (n = 2) mice are shown as mean ± SEM. * p

    Article Snippet: Markers for mouse endothelial cells were anti-CD31 (PECAM; Biolegend, San Diego, CA, USA); Alexa Fluor® 647-conjugated-anti-CD34 (found on normal endothelial cells and stem cells [ ]), PE-anti-CD105 (Endoglin, a regulatory component of TGF-β receptor specific for tumor angiogenesis [ ]), Alexa Fluor® 647-anti-CD106 (vascular cell adhesion molecule; VCAM) expressed on activated endothelial cells; all from eBioscience, San Diego, CA, USA.

    Techniques: In Vivo, In Vitro, MTT Assay, Mouse Assay, Imaging

    Antigen presentation on LSECs in the liver is sufficient to drive liver failure. a Schematic of various bm-chimeric animals that have a Kb-restricted antigen presentation on myeloid cells only (Tie2-Kb→DBA/2, black), myeloid and endothelial cells (Tie2-Kb→Tie2-Kb, red), or myeloid cells and hepatocytes (Tie2-Kb→Crp-Kb, blue). b – d ALT serum levels ( b ), percentage of loss of body weight ( c ) and absolute numbers of OT-I cells recovered from the spleen of the various bm-chimeric animals ( d ). e Immunofluorescence images showing CD31 and CD8 staining of the livers of various bm-chimeric mice on day 4 post infection. f Quantification of vessel surface of the various bm-chimeric mice. Data are representative of three independent experiments ( n ≥ 3). Scale bars: 80 µm. Error bars indicate the mean ± SD. Comparison between groups was calculated using one-way ANOVA with Turkey’s multiple comparison post test. n.s., not significant; * p ≤ 0.05; ** p

    Journal: Nature Communications

    Article Title: Perforin inhibition protects from lethal endothelial damage during fulminant viral hepatitis

    doi: 10.1038/s41467-018-07213-x

    Figure Lengend Snippet: Antigen presentation on LSECs in the liver is sufficient to drive liver failure. a Schematic of various bm-chimeric animals that have a Kb-restricted antigen presentation on myeloid cells only (Tie2-Kb→DBA/2, black), myeloid and endothelial cells (Tie2-Kb→Tie2-Kb, red), or myeloid cells and hepatocytes (Tie2-Kb→Crp-Kb, blue). b – d ALT serum levels ( b ), percentage of loss of body weight ( c ) and absolute numbers of OT-I cells recovered from the spleen of the various bm-chimeric animals ( d ). e Immunofluorescence images showing CD31 and CD8 staining of the livers of various bm-chimeric mice on day 4 post infection. f Quantification of vessel surface of the various bm-chimeric mice. Data are representative of three independent experiments ( n ≥ 3). Scale bars: 80 µm. Error bars indicate the mean ± SD. Comparison between groups was calculated using one-way ANOVA with Turkey’s multiple comparison post test. n.s., not significant; * p ≤ 0.05; ** p

    Article Snippet: To assess CD31 staining of the liver vasculature, 10 µg of fluorescent anti-CD31 antibody (AF647-labeled, clone Mec13.3, Biolegend) was injected i.v. 15 min before explantation of the liver.

    Techniques: Immunofluorescence, Staining, Mouse Assay, Infection

    Therapeutic treatment with perforin-1 inhibitor ameliorates the liver damage. a – c Serum ALT activity ( a ), percentage of loss of body weight ( b ), and survival curves ( c ) of wt mice with T cell-mediated hepatitis that received perforin-1 inhibitor or solvent treatment starting at 24 h post infection. d Immunofluorescence images of the mouse livers treated as in a – c showing CD31, CD8, and virally expressed GFP. Data are representative of two independent experiments ( n ≥ 5). Scale bars: 80 µm. Error bars indicate the mean ± SD. Comparison between groups was calculated using unpaired Student’s t test. * p ≤ 0.05; ** p

    Journal: Nature Communications

    Article Title: Perforin inhibition protects from lethal endothelial damage during fulminant viral hepatitis

    doi: 10.1038/s41467-018-07213-x

    Figure Lengend Snippet: Therapeutic treatment with perforin-1 inhibitor ameliorates the liver damage. a – c Serum ALT activity ( a ), percentage of loss of body weight ( b ), and survival curves ( c ) of wt mice with T cell-mediated hepatitis that received perforin-1 inhibitor or solvent treatment starting at 24 h post infection. d Immunofluorescence images of the mouse livers treated as in a – c showing CD31, CD8, and virally expressed GFP. Data are representative of two independent experiments ( n ≥ 5). Scale bars: 80 µm. Error bars indicate the mean ± SD. Comparison between groups was calculated using unpaired Student’s t test. * p ≤ 0.05; ** p

    Article Snippet: To assess CD31 staining of the liver vasculature, 10 µg of fluorescent anti-CD31 antibody (AF647-labeled, clone Mec13.3, Biolegend) was injected i.v. 15 min before explantation of the liver.

    Techniques: Activity Assay, Mouse Assay, Infection, Immunofluorescence

    Disturbed endothelial integrity of liver sinusoids is an essential component of fatality. a Schematic showing the approach to induce transient, TNF-mediated hepatitis after adenoviral infection. b Serum ALT activity of mice infected with 2 × 10 9 PFU AdGOL and injected with 400 ng TNF i.v. on day 2 post infection. Samples were taken 4 h after injection of TNF. c Light microscopic images of the livers of mice with acute liver failure (T cell-mediated) or with transient hepatitis (cytokine-mediated) showing Evans Blue leakage. Evans Blue dye was injected i.v. on day 3 post OT-I cell transfer and infection with AdGOL or 4 h post TNF injection. d – f Immunofluorescence images showing CD31 staining ( d ) and quantification of vessel surfaces ( e , f ) of the livers with T cell- or cytokine-mediated hepatitis. g Light microscopic images of the livers of wt mice adoptively transferred with wt or Prf1 −/− OT-I cells and infection with AdGOL on day 0. Some mice were injected with FasL- or TNF-blocking antibodies on day 2 and Evans Blue dye was injected on day 3. Scale bars: 200 µm ( c , g ) or 100 µm ( d ). Data are representative of two ( b ) or three independent experiments ( n = 5 ( b ) or n = 3). Error bars indicate the mean ± SD. Comparison between groups was calculated using unpaired Student’s t test. n.s., not significant; ** p

    Journal: Nature Communications

    Article Title: Perforin inhibition protects from lethal endothelial damage during fulminant viral hepatitis

    doi: 10.1038/s41467-018-07213-x

    Figure Lengend Snippet: Disturbed endothelial integrity of liver sinusoids is an essential component of fatality. a Schematic showing the approach to induce transient, TNF-mediated hepatitis after adenoviral infection. b Serum ALT activity of mice infected with 2 × 10 9 PFU AdGOL and injected with 400 ng TNF i.v. on day 2 post infection. Samples were taken 4 h after injection of TNF. c Light microscopic images of the livers of mice with acute liver failure (T cell-mediated) or with transient hepatitis (cytokine-mediated) showing Evans Blue leakage. Evans Blue dye was injected i.v. on day 3 post OT-I cell transfer and infection with AdGOL or 4 h post TNF injection. d – f Immunofluorescence images showing CD31 staining ( d ) and quantification of vessel surfaces ( e , f ) of the livers with T cell- or cytokine-mediated hepatitis. g Light microscopic images of the livers of wt mice adoptively transferred with wt or Prf1 −/− OT-I cells and infection with AdGOL on day 0. Some mice were injected with FasL- or TNF-blocking antibodies on day 2 and Evans Blue dye was injected on day 3. Scale bars: 200 µm ( c , g ) or 100 µm ( d ). Data are representative of two ( b ) or three independent experiments ( n = 5 ( b ) or n = 3). Error bars indicate the mean ± SD. Comparison between groups was calculated using unpaired Student’s t test. n.s., not significant; ** p

    Article Snippet: To assess CD31 staining of the liver vasculature, 10 µg of fluorescent anti-CD31 antibody (AF647-labeled, clone Mec13.3, Biolegend) was injected i.v. 15 min before explantation of the liver.

    Techniques: Infection, Activity Assay, Mouse Assay, Injection, Immunofluorescence, Staining, Blocking Assay