Journal: International Journal of Immunopathology and Pharmacology
Article Title: Astragalus polysaccharide alleviates LPS-induced inflammation injury by regulating miR-127 in H9c2 cardiomyoblasts
Figure Lengend Snippet: APS reduced LPS-induced inflammation injury by down-regulating miR-127 and inhibiting NF-κB and JNK and promoting PI3K/AKT signaling pathways in H9c2 cells. (a) H9c2 cells were treated with LPS or co-treated with APS and LPS, and then expression levels of miR-127 in H9c2 cells were measured by qRT-PCR. (b)–(d) Protein expression levels of p65/p-p65, lκBα/p-lκBα JNK/p-JNK, c-Jun/p-c-Jun, PI3K/p-PI3K, and AKT/p-AKT were detected by western blot. Different letters above the bars (a, b, c) indicate that the means of different groups were significantly different ( P
Article Snippet: The primary antibodies used in this study included anti-Bax (ab32503, 1:1000), anti-pro-Caspase3 (ab44976, 1:500), anti-cleaved-Caspase3 (ab13847, 1:500), anti-Caspase9 (ab25758, 1:200), anti-IL-6 (ab9770, 1:5000), anti-IL-8 (ab7747, 1:10), anti-TNF-α (ab6671, 1:1000), anti-p65 (ab16502, 1:2000), anti-toll-like receptor 4 (anti-TLR4, ab13556, 1:500), anti-p-p65 (ab86299, 1:2000), anti-IκBα (ab7217, 1:2000), anti-JNK (ab179461, 1:1000), anti-p-JNK (ab124956, 1:5000), anti-c-Jun (ab32137, 1:1000) and anti-p-c-Jun (ab32385, 1:1000), anti-PI3K (ab191606, 1:1000), anti-p-PI3K (ab182651, 1:1000), anti-AKT (ab32505, 1:2000), anti-p-AKT (ab131443, 1:1000), all purchased from Abcam (Cambridge, UK).
Techniques: Expressing, Quantitative RT-PCR, Western Blot