Journal: International Journal of Molecular Sciences
Article Title: The Cyanthin Diterpenoid and Sesterterpene Constituents of Hericium erinaceus Mycelium Ameliorate Alzheimer’s Disease-Related Pathologies in APP/PS1 Transgenic Mice
Figure Lengend Snippet: HE-A and HE-S reduce glial activation in APP/PS1 mice. APP/PS1 transgenic mice were orally administered with vehicle (Veh), HE-A or HE-S for 30 days ( n = 8 each). Amyloid plaques were detected by immunohistochemical staining with AB-10 antibody (blue). Microglia and reactive astrocytes were detected by immunohistochemical staining with Iba-1 antibody (red) and glial fibrillary acidic protein (GFAP) antibody (green), respectively. The representative immunostaining images of clusters in section of parietal cortical area and temporal cortical area are shown ( A ). Scale bar: 500 µm. The number of microglial and astroglial cluster in the section of cerebral hemisphere is calculated by image analysis software and shown in ( B ). The representative immunostaining images of glial cells without associated with plaque in Cornu Amonis (CA)1, CA3, dentate gyrus (DG) were shown in ( C ). The image in WT mice ( n = 5) is also compared. Sale bar: 100 µm. The immunoreactivity of Iba-1 and GFAP in the CA1, CA3, and DG is calculated by image analysis software and shown in ( D ). The level of GFAP and Iba-1 in homogenates were analyzed by immunoblotting (E, F). The protein level in WT mice ( n = 5) is also compared. Representative immunoblots are shown at (E). The ratio of CTF-α and −β to β-actin is presented as percentage of Veh group. The ratio of IDE to β-actin is presented as percentage of WT group. The results are the mean ± S.E.M. Significant differences between Veh group and the other groups are indicated by *, p
Article Snippet: The primary antibodies used were as follows: mouse anti-GFAP antibody (GFAP, Millipore, MAB360, 2041104), mouse anti-APP antibody (Millipore, MAB348, 25030193), rabbit anti-CTF antibody (Millipore, AB5352, 22051154), rabbit anti-neprilysin (NEP) antibody (Millipore, AB5458, LV1423485), rabbit anti-IDE antibody (Millipore, AB9210, 2769024), mouse anti-β-actin antibody (Millipore, MAB, LV1460528) and goat anti-Iba-1 antibody (Abcam, ab5076, GR268568-3).
Techniques: Activation Assay, Mouse Assay, Transgenic Assay, Immunohistochemistry, Staining, Immunostaining, Software, Western Blot