Abcam
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Image Search Results

Journal: PLoS ONE
Article Title: Ubiquitin Accumulation on Disease Associated Protein Aggregates Is Correlated with Nuclear Ubiquitin Depletion, Histone De-Ubiquitination and Impaired DNA Damage Response
doi: 10.1371/journal.pone.0169054
Figure Lengend Snippet: U2OS cells were transiently transfected with Htt-Q91-Cherry and fixed for immunofluorescence with an antibody that identifies ubiquitinated H2B (A) and ubiquitinated H2AX The staining of the IB with the Ubi-H2B and Ubi-H2AX antibodies is likely due to cross reaction of these antibodies with the high concentrations of ubiquitin on the aggregates (B) . Cells were either imaged under the microscope (A,B) or analyzed in bulk by flow cytometry. The red bars indicate cells that lack aggregates and the blue bars cells with aggregates. The presented experiment is a representative of three repeats (C) .
Article Snippet: The following antibodies were used: Mouse anti Mono and poly Ubiquitinylated conjugates FK2 (ENZO), Mouse anti Phospho histone H2A.X (Ser139) (Millipore), Mouse anti
Techniques: Transfection, Immunofluorescence, Staining, Microscopy, Flow Cytometry

Journal: PLoS ONE
Article Title: Ubiquitin Accumulation on Disease Associated Protein Aggregates Is Correlated with Nuclear Ubiquitin Depletion, Histone De-Ubiquitination and Impaired DNA Damage Response
doi: 10.1371/journal.pone.0169054
Figure Lengend Snippet: U2OS cells expressing an inducible Htt-Q91-Cherry vector were induced with dox. Cells were fixed and stained with Fk2 antibodies that identify ubiquitinated proteins B, antibodies against ubiquitinated H2B (B) and ubiquitinated H2AX (C) . Cells were imaged under the microscope (B,C) . Several hundred cells in multiple fields were photographed and quantified by ImageJ. The red bars indicate cells that lack aggregates (normalized to 1) and the blue bars cells with aggregates, error bars are SE (D) .
Article Snippet: The following antibodies were used: Mouse anti Mono and poly Ubiquitinylated conjugates FK2 (ENZO), Mouse anti Phospho histone H2A.X (Ser139) (Millipore), Mouse anti
Techniques: Expressing, Plasmid Preparation, Staining, Microscopy