anti trpv4 extracellular antibody Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Alomone Labs anti trpv4 extracellular antibody
    PAR2 and <t>TRPV4</t> expression in the hippocampus. Immunohistochemistry discloses the expression of PAR2 and TRPV4 in the hippocampus. A comparable expression pattern is observed: high levels of PAR2 and TRPV4 are detected in CA1 stratum pyramidale (pcl, pyramidal cell layer; oriens, stratum oriens; rad, stratum radiatum; la-mol, stratum lacunosum-moleculare). No pronounced colocalization between PAR2 and GFAP was detected. Scale bars: 100 and 10 μm, n = 9 slices out of three animals.
    Anti Trpv4 Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti trpv4 extracellular antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti trpv4 extracellular antibody - by Bioz Stars, 2022-10
    93/100 stars
      Buy from Supplier

    95
    Alomone Labs anti mouse trpv4
    Transfection of large mouse cholangiocytes (MLC) with plasmid for transient receptor potential vanilloid member 4 <t>(TRPV4)</t> small interfering (si)RNA decreases protein and RNA expression. A : representative Western blot demonstrating change in TRPV4 protein levels in cells transfected with nontargeting siRNA (scramble) and cells transfected with TRPV4 siRNA. β-Actin was used as loading control. B : cumulative data demonstrating change in TRPV4 protein ( left ) and mRNA ( right ) levels in control cells, cells transfected with nontargeting siRNA (scramble), and cells transfected with TRPV4 siRNA (* P
    Anti Mouse Trpv4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse trpv4/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mouse trpv4 - by Bioz Stars, 2022-10
    95/100 stars
      Buy from Supplier

    Image Search Results


    PAR2 and TRPV4 expression in the hippocampus. Immunohistochemistry discloses the expression of PAR2 and TRPV4 in the hippocampus. A comparable expression pattern is observed: high levels of PAR2 and TRPV4 are detected in CA1 stratum pyramidale (pcl, pyramidal cell layer; oriens, stratum oriens; rad, stratum radiatum; la-mol, stratum lacunosum-moleculare). No pronounced colocalization between PAR2 and GFAP was detected. Scale bars: 100 and 10 μm, n = 9 slices out of three animals.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Protease Activated Receptor 2 (PAR2) Induces Long-Term Depression in the Hippocampus through Transient Receptor Potential Vanilloid 4 (TRPV4)

    doi: 10.3389/fnmol.2017.00042

    Figure Lengend Snippet: PAR2 and TRPV4 expression in the hippocampus. Immunohistochemistry discloses the expression of PAR2 and TRPV4 in the hippocampus. A comparable expression pattern is observed: high levels of PAR2 and TRPV4 are detected in CA1 stratum pyramidale (pcl, pyramidal cell layer; oriens, stratum oriens; rad, stratum radiatum; la-mol, stratum lacunosum-moleculare). No pronounced colocalization between PAR2 and GFAP was detected. Scale bars: 100 and 10 μm, n = 9 slices out of three animals.

    Article Snippet: Immunohistochemistry The following primary antibodies were used for immunodetection: goat anti-PAR2 (sc-8205, Santa Cruz, 1:25), rabbit anti-TRPV4 (ACC-124, Alomone Labs, 1:50), rabbit anti-PAR2 (APR-032, Alomone Labs 1:500) and mouse anti-GFAP (G3893, Sigma-Aldrich, 1:2000).

    Techniques: Expressing, Immunohistochemistry

    PAR2 induces LTD through the activation of TRPV4. (A) Application of TRPV4-agonist (2 μM RN1747) causes LTD. (B) Removal of the TRPV4-agonist (2 μM RN1747) following induction of LTD does not affect the stability of synaptic depression. (C) In presence of the TRPV4-antagonist (10 μM RN1734) the TRPV4-agonist is not able to induce synaptic depression. (D) In a two pathways experimental setting, low frequency stimulation (LFS, 1 Hz, 900 pulses) and TRPV4-agonist application induce similar levels of LTD. (E) LFS-induced LTD is not blocked by the TRPV4-antagonist. (F) Application of PAR2-agonist (10 μM AC55541) in presence of a TRPV4-antagonist (10 μM RN1734) blocks PAR2-induced LTD. (G) Application of TRPV4-agonist (2 μM RN1747) in presence of PAR2-antagonist (50 μM FSLLRY-NH 2 ) does not affect TRPV4-induced LTD. (H) Once PAR2-agonist mediated LTD is established, the TRPV4-agonist (2 μM RN1747) does not further de-potentiate a second pathway at adjusted response level (upward arrow). Averaged EPSP are plotted versus time. Representative traces at indicated times (a, b) are shown on top of each section, n = 12 slices for each experiments, refer to text for statistics.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Protease Activated Receptor 2 (PAR2) Induces Long-Term Depression in the Hippocampus through Transient Receptor Potential Vanilloid 4 (TRPV4)

    doi: 10.3389/fnmol.2017.00042

    Figure Lengend Snippet: PAR2 induces LTD through the activation of TRPV4. (A) Application of TRPV4-agonist (2 μM RN1747) causes LTD. (B) Removal of the TRPV4-agonist (2 μM RN1747) following induction of LTD does not affect the stability of synaptic depression. (C) In presence of the TRPV4-antagonist (10 μM RN1734) the TRPV4-agonist is not able to induce synaptic depression. (D) In a two pathways experimental setting, low frequency stimulation (LFS, 1 Hz, 900 pulses) and TRPV4-agonist application induce similar levels of LTD. (E) LFS-induced LTD is not blocked by the TRPV4-antagonist. (F) Application of PAR2-agonist (10 μM AC55541) in presence of a TRPV4-antagonist (10 μM RN1734) blocks PAR2-induced LTD. (G) Application of TRPV4-agonist (2 μM RN1747) in presence of PAR2-antagonist (50 μM FSLLRY-NH 2 ) does not affect TRPV4-induced LTD. (H) Once PAR2-agonist mediated LTD is established, the TRPV4-agonist (2 μM RN1747) does not further de-potentiate a second pathway at adjusted response level (upward arrow). Averaged EPSP are plotted versus time. Representative traces at indicated times (a, b) are shown on top of each section, n = 12 slices for each experiments, refer to text for statistics.

    Article Snippet: Immunohistochemistry The following primary antibodies were used for immunodetection: goat anti-PAR2 (sc-8205, Santa Cruz, 1:25), rabbit anti-TRPV4 (ACC-124, Alomone Labs, 1:50), rabbit anti-PAR2 (APR-032, Alomone Labs 1:500) and mouse anti-GFAP (G3893, Sigma-Aldrich, 1:2000).

    Techniques: Activation Assay

    TRPV4-mediated LTD depends on NMDAR-activity. (A) Similar to PAR2-induced LTD (c.f., Figures 1G,H ), the NMDAR-antagonist (50 μM APV) blocks TRPV4 (2 μM RN1747)-induced LTD, while (B) application of a TRPV4-agonist (2 μM RN1747) induces LTD in presence of the mGluR-antagonist (200 μM MCGP). Averaged EPSP are plotted versus time. Representative traces at indicated times (a, b) are shown on top of each section.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Protease Activated Receptor 2 (PAR2) Induces Long-Term Depression in the Hippocampus through Transient Receptor Potential Vanilloid 4 (TRPV4)

    doi: 10.3389/fnmol.2017.00042

    Figure Lengend Snippet: TRPV4-mediated LTD depends on NMDAR-activity. (A) Similar to PAR2-induced LTD (c.f., Figures 1G,H ), the NMDAR-antagonist (50 μM APV) blocks TRPV4 (2 μM RN1747)-induced LTD, while (B) application of a TRPV4-agonist (2 μM RN1747) induces LTD in presence of the mGluR-antagonist (200 μM MCGP). Averaged EPSP are plotted versus time. Representative traces at indicated times (a, b) are shown on top of each section.

    Article Snippet: Immunohistochemistry The following primary antibodies were used for immunodetection: goat anti-PAR2 (sc-8205, Santa Cruz, 1:25), rabbit anti-TRPV4 (ACC-124, Alomone Labs, 1:50), rabbit anti-PAR2 (APR-032, Alomone Labs 1:500) and mouse anti-GFAP (G3893, Sigma-Aldrich, 1:2000).

    Techniques: Activity Assay

    Transfection of large mouse cholangiocytes (MLC) with plasmid for transient receptor potential vanilloid member 4 (TRPV4) small interfering (si)RNA decreases protein and RNA expression. A : representative Western blot demonstrating change in TRPV4 protein levels in cells transfected with nontargeting siRNA (scramble) and cells transfected with TRPV4 siRNA. β-Actin was used as loading control. B : cumulative data demonstrating change in TRPV4 protein ( left ) and mRNA ( right ) levels in control cells, cells transfected with nontargeting siRNA (scramble), and cells transfected with TRPV4 siRNA (* P

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Mechanosensor transient receptor potential vanilloid member 4 (TRPV4) regulates mouse cholangiocyte secretion and bile formation

    doi: 10.1152/ajpgi.00176.2019

    Figure Lengend Snippet: Transfection of large mouse cholangiocytes (MLC) with plasmid for transient receptor potential vanilloid member 4 (TRPV4) small interfering (si)RNA decreases protein and RNA expression. A : representative Western blot demonstrating change in TRPV4 protein levels in cells transfected with nontargeting siRNA (scramble) and cells transfected with TRPV4 siRNA. β-Actin was used as loading control. B : cumulative data demonstrating change in TRPV4 protein ( left ) and mRNA ( right ) levels in control cells, cells transfected with nontargeting siRNA (scramble), and cells transfected with TRPV4 siRNA (* P

    Article Snippet: After blocking, immunoblots were incubated overnight with anti-mouse TRPV4 (1:200, Alomone Laboratories) This was followed by incubation with peroxidase-conjugated goat antirabbit antibody (dilution 1:10,000, Jackson Immuno Research Laboratories) and visualized with an ECL+ detection kit (GE Healthcare).

    Techniques: Transfection, Plasmid Preparation, RNA Expression, Western Blot

    Flow-stimulated increases in intracellular calcium concentration ([Ca 2+ ] i ) in large mouse cholangiocytes (MLC). Representative Ca 2+ fluorescence in response to fluid flow. MLC on coverglass were loaded with fura-2 AM and exposed to flow (2 mL/min; shear = 0.64 dyne/cm 2 ); y -axis values represent the ratio of fluorescence at 340 and 380 nm; x -axis represents time (bar = 100 s). Control cells shown ( A ) vs. cells exposed to HC-067047 (HC, 10 µM; B ) and after transfection with transient receptor potential vanilloid member 4 (TRPV4) small interfering (si)RNA ( C ). Cells were exposed to the Ca 2+ ionophore ionomycin, as an internal control. D : cumulative data demonstrating the change in [Ca 2+ ] i in response to fluid flow (shear = 0.64 dyne/cm 2 ) in control cells ( n = 78) and in the presence of HC-067047 (HC, n = 61), or after transfection with TRPV4 siRNA ( n = 49). Values represent change in fluorescence ratio 340/380. Maximal increase vs. basal # P ≤ 0.01; inhibitor HC067047 vs. control ** P ≤ 0.01; * P

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Mechanosensor transient receptor potential vanilloid member 4 (TRPV4) regulates mouse cholangiocyte secretion and bile formation

    doi: 10.1152/ajpgi.00176.2019

    Figure Lengend Snippet: Flow-stimulated increases in intracellular calcium concentration ([Ca 2+ ] i ) in large mouse cholangiocytes (MLC). Representative Ca 2+ fluorescence in response to fluid flow. MLC on coverglass were loaded with fura-2 AM and exposed to flow (2 mL/min; shear = 0.64 dyne/cm 2 ); y -axis values represent the ratio of fluorescence at 340 and 380 nm; x -axis represents time (bar = 100 s). Control cells shown ( A ) vs. cells exposed to HC-067047 (HC, 10 µM; B ) and after transfection with transient receptor potential vanilloid member 4 (TRPV4) small interfering (si)RNA ( C ). Cells were exposed to the Ca 2+ ionophore ionomycin, as an internal control. D : cumulative data demonstrating the change in [Ca 2+ ] i in response to fluid flow (shear = 0.64 dyne/cm 2 ) in control cells ( n = 78) and in the presence of HC-067047 (HC, n = 61), or after transfection with TRPV4 siRNA ( n = 49). Values represent change in fluorescence ratio 340/380. Maximal increase vs. basal # P ≤ 0.01; inhibitor HC067047 vs. control ** P ≤ 0.01; * P

    Article Snippet: After blocking, immunoblots were incubated overnight with anti-mouse TRPV4 (1:200, Alomone Laboratories) This was followed by incubation with peroxidase-conjugated goat antirabbit antibody (dilution 1:10,000, Jackson Immuno Research Laboratories) and visualized with an ECL+ detection kit (GE Healthcare).

    Techniques: Concentration Assay, Fluorescence, Transfection

    Specific agonists of transient receptor potential vanilloid member 4 (TRPV4) activate currents and increase intracellular calcium concentration ([Ca 2+ ] i ) in large mouse cholangiocytes (MLC). A, top : representative whole cell recording. The specific TRPV4 agonist 4α -phorbol-12,13-didecanoate (4αPDD; 1 µM) activates whole cell currents. Currents measured at +100 mV (○) and at −100 mV (●) are shown. Voltage protocol −100 mV to +100 mV ramp every 5 s. Bottom : cells on coverglass were loaded with fura-2 AM, washed with PBS, and exposed to 4αPDD (1 µM); y -axis values represent the ratio of fluorescence at 340 and at 380 nm. B, top : the specific TRPV4 agonist GSK (100 nM) activates whole cell currents. Currents measured at +100 mv (○) and at −100 mV (●) are shown. Bottom : fura-2-loaded MLC cells were exposed to glycogen synthase kinase (GSK; 100 nM). The y -axis values represent the ratio of fluorescence at 340 and at 380 nm. C, top : cumulative data demonstrating maximal current density (pA/pF) measured at +100 mV in response to 4αPDD or GSK. Currents were significantly inhibited by ruthenium red (RR, 10 µM, n = 9), HC-067047 (HC, 10 µM, n = 16), and after transfection with TRPV4 small interfering (si)RNA ( n = 8). Bottom : cumulative data demonstrating the change in [Ca 2+ ] i in response to 4αPDD or GSK in control cells ( n = 78) and in the presence of RR ( n = 46), HC ( n = 54), or after transfection with TRPV4 siRNA ( n = 27). Values represent relative change in fluorescence ratio 340/380. Maximal increase vs. basal ** P ≤ 0.01, inhibitor vs. control, * P ≤ 0.05, vs. control ** P ≤ 0.01, * P

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Mechanosensor transient receptor potential vanilloid member 4 (TRPV4) regulates mouse cholangiocyte secretion and bile formation

    doi: 10.1152/ajpgi.00176.2019

    Figure Lengend Snippet: Specific agonists of transient receptor potential vanilloid member 4 (TRPV4) activate currents and increase intracellular calcium concentration ([Ca 2+ ] i ) in large mouse cholangiocytes (MLC). A, top : representative whole cell recording. The specific TRPV4 agonist 4α -phorbol-12,13-didecanoate (4αPDD; 1 µM) activates whole cell currents. Currents measured at +100 mV (○) and at −100 mV (●) are shown. Voltage protocol −100 mV to +100 mV ramp every 5 s. Bottom : cells on coverglass were loaded with fura-2 AM, washed with PBS, and exposed to 4αPDD (1 µM); y -axis values represent the ratio of fluorescence at 340 and at 380 nm. B, top : the specific TRPV4 agonist GSK (100 nM) activates whole cell currents. Currents measured at +100 mv (○) and at −100 mV (●) are shown. Bottom : fura-2-loaded MLC cells were exposed to glycogen synthase kinase (GSK; 100 nM). The y -axis values represent the ratio of fluorescence at 340 and at 380 nm. C, top : cumulative data demonstrating maximal current density (pA/pF) measured at +100 mV in response to 4αPDD or GSK. Currents were significantly inhibited by ruthenium red (RR, 10 µM, n = 9), HC-067047 (HC, 10 µM, n = 16), and after transfection with TRPV4 small interfering (si)RNA ( n = 8). Bottom : cumulative data demonstrating the change in [Ca 2+ ] i in response to 4αPDD or GSK in control cells ( n = 78) and in the presence of RR ( n = 46), HC ( n = 54), or after transfection with TRPV4 siRNA ( n = 27). Values represent relative change in fluorescence ratio 340/380. Maximal increase vs. basal ** P ≤ 0.01, inhibitor vs. control, * P ≤ 0.05, vs. control ** P ≤ 0.01, * P

    Article Snippet: After blocking, immunoblots were incubated overnight with anti-mouse TRPV4 (1:200, Alomone Laboratories) This was followed by incubation with peroxidase-conjugated goat antirabbit antibody (dilution 1:10,000, Jackson Immuno Research Laboratories) and visualized with an ECL+ detection kit (GE Healthcare).

    Techniques: Concentration Assay, Fluorescence, Transfection