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  • 80
    Santa Cruz Biotechnology synpo
    Synpo, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Santa Cruz Biotechnology goat anti synpo
    Goat Anti Synpo, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Santa Cruz Biotechnology anti synpo
    Anti Synpo, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Synaptic Systems rabbit anti synaptoporin
    Knockdown of A 2A Rs in dentate granule cells reduces status epilepticus-induced hippocampal mossy fiber sprouting. ( a ) Lentivirus encoding EGFP and shRNA-Control (shCTR) or shRNA-A 2A R (shA 2A R) were injected into the hippocampal dentate gyrus (DG) in the left or in the right hemisphere. After 10 days, rats were injected with saline (Control group) or pilocarpine (Status epilepticus group). All rats were administered with scopolamine methylbromide (2 mg/kg, i.p. ) 30 min before. Only animals reaching at least stage 4 seizures were considered. ( b ) After 55 days, mossy fiber (MF) sprouting was assessed by immunohistochemical analysis of <t>synaptoporin</t> (MF terminals marker). Representative images of the immunolabelling of synaptoporin and neuronal nuclei (NeuN) showing that in rats that experienced status epilepticus (SE) it was observed synaptoporin immunoreactivity in the inner molecular layer (IML) of the DG (white arrows), which was not observed in control rats. Scale bar, 300 μm. ( c ) Representative image showing a DG segment infected with lentivirus encoding shCTR from a rat that experienced SE, immunolabelled with synaptoporin and NeuN and displaying EGFP + -dentate granule cells. Scale bar, 100 µm. ( d ) Schematic illustration of the quantitative method used to analyse the extent of MF sprouting. A Δsprouting ratio for each acquired image with obviously different EGFP + -cell density in adjacent areas was calculated by measuring the incremental proportion of the synaptoporin (cyan) puncta density (PD2 to PD1) in the IML vs. the incremental proportion of EGFP + -neuron density (ED2 to ED1) in the granule cell layer (GCL), defined by NeuN immunoreactivity. ( e ) The brain sections from shCTR- or shRNA-A 2A R-injected DG from control animals displayed no synaptoporin staining (cyan) in the IML. In SE group, shA 2A R-injected DG sections displayed lower synaptoporin puncta densities in the IML region corresponding to the higher EGFP + -cell density region in GCL which was not observed in shCTR-injected DG. Scale bar, 25 μm. ( f ) Δsprouting ratio in shA 2A R-injected DG and shCTR-injected DG in each individual animal that had experienced SE ( left ), supporting a significant lower Δsprouting in shA 2A R-injected DG vs. shCTR-injected DG (right). The data are median and interquartile range ( left graph) or mean ± SEM ( right graph). * P
    Rabbit Anti Synaptoporin, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Knockdown of A 2A Rs in dentate granule cells reduces status epilepticus-induced hippocampal mossy fiber sprouting. ( a ) Lentivirus encoding EGFP and shRNA-Control (shCTR) or shRNA-A 2A R (shA 2A R) were injected into the hippocampal dentate gyrus (DG) in the left or in the right hemisphere. After 10 days, rats were injected with saline (Control group) or pilocarpine (Status epilepticus group). All rats were administered with scopolamine methylbromide (2 mg/kg, i.p. ) 30 min before. Only animals reaching at least stage 4 seizures were considered. ( b ) After 55 days, mossy fiber (MF) sprouting was assessed by immunohistochemical analysis of synaptoporin (MF terminals marker). Representative images of the immunolabelling of synaptoporin and neuronal nuclei (NeuN) showing that in rats that experienced status epilepticus (SE) it was observed synaptoporin immunoreactivity in the inner molecular layer (IML) of the DG (white arrows), which was not observed in control rats. Scale bar, 300 μm. ( c ) Representative image showing a DG segment infected with lentivirus encoding shCTR from a rat that experienced SE, immunolabelled with synaptoporin and NeuN and displaying EGFP + -dentate granule cells. Scale bar, 100 µm. ( d ) Schematic illustration of the quantitative method used to analyse the extent of MF sprouting. A Δsprouting ratio for each acquired image with obviously different EGFP + -cell density in adjacent areas was calculated by measuring the incremental proportion of the synaptoporin (cyan) puncta density (PD2 to PD1) in the IML vs. the incremental proportion of EGFP + -neuron density (ED2 to ED1) in the granule cell layer (GCL), defined by NeuN immunoreactivity. ( e ) The brain sections from shCTR- or shRNA-A 2A R-injected DG from control animals displayed no synaptoporin staining (cyan) in the IML. In SE group, shA 2A R-injected DG sections displayed lower synaptoporin puncta densities in the IML region corresponding to the higher EGFP + -cell density region in GCL which was not observed in shCTR-injected DG. Scale bar, 25 μm. ( f ) Δsprouting ratio in shA 2A R-injected DG and shCTR-injected DG in each individual animal that had experienced SE ( left ), supporting a significant lower Δsprouting in shA 2A R-injected DG vs. shCTR-injected DG (right). The data are median and interquartile range ( left graph) or mean ± SEM ( right graph). * P

    Journal: Scientific Reports

    Article Title: Adenosine A2A receptors control synaptic remodeling in the adult brain

    doi: 10.1038/s41598-022-18884-4

    Figure Lengend Snippet: Knockdown of A 2A Rs in dentate granule cells reduces status epilepticus-induced hippocampal mossy fiber sprouting. ( a ) Lentivirus encoding EGFP and shRNA-Control (shCTR) or shRNA-A 2A R (shA 2A R) were injected into the hippocampal dentate gyrus (DG) in the left or in the right hemisphere. After 10 days, rats were injected with saline (Control group) or pilocarpine (Status epilepticus group). All rats were administered with scopolamine methylbromide (2 mg/kg, i.p. ) 30 min before. Only animals reaching at least stage 4 seizures were considered. ( b ) After 55 days, mossy fiber (MF) sprouting was assessed by immunohistochemical analysis of synaptoporin (MF terminals marker). Representative images of the immunolabelling of synaptoporin and neuronal nuclei (NeuN) showing that in rats that experienced status epilepticus (SE) it was observed synaptoporin immunoreactivity in the inner molecular layer (IML) of the DG (white arrows), which was not observed in control rats. Scale bar, 300 μm. ( c ) Representative image showing a DG segment infected with lentivirus encoding shCTR from a rat that experienced SE, immunolabelled with synaptoporin and NeuN and displaying EGFP + -dentate granule cells. Scale bar, 100 µm. ( d ) Schematic illustration of the quantitative method used to analyse the extent of MF sprouting. A Δsprouting ratio for each acquired image with obviously different EGFP + -cell density in adjacent areas was calculated by measuring the incremental proportion of the synaptoporin (cyan) puncta density (PD2 to PD1) in the IML vs. the incremental proportion of EGFP + -neuron density (ED2 to ED1) in the granule cell layer (GCL), defined by NeuN immunoreactivity. ( e ) The brain sections from shCTR- or shRNA-A 2A R-injected DG from control animals displayed no synaptoporin staining (cyan) in the IML. In SE group, shA 2A R-injected DG sections displayed lower synaptoporin puncta densities in the IML region corresponding to the higher EGFP + -cell density region in GCL which was not observed in shCTR-injected DG. Scale bar, 25 μm. ( f ) Δsprouting ratio in shA 2A R-injected DG and shCTR-injected DG in each individual animal that had experienced SE ( left ), supporting a significant lower Δsprouting in shA 2A R-injected DG vs. shCTR-injected DG (right). The data are median and interquartile range ( left graph) or mean ± SEM ( right graph). * P

    Article Snippet: Sections were then incubated overnight at 4 °C with the following primary antibodies in blocking solution: rat anti-BrdU (1:200; AbD Serotec, Cat#OBT0030G, RRID: AB_609567), rabbit anti-GFAP (1:1000; Milipore, Cat#AB5804, RRID:AB_305124) and mouse anti-NeuN (1:400; Milipore, Cat#MAB377, RRID:AB_2298772) or rabbit anti-synaptoporin (1:500, Synaptic Systems, Cat#102002, RRID: AB_312851).

    Techniques: shRNA, Injection, Immunohistochemistry, Marker, Infection, Staining

    MF sprouting analysis by synaptoporin puncta density in lentiviral-infected DG

    Journal: Scientific Reports

    Article Title: Adenosine A2A receptors control synaptic remodeling in the adult brain

    doi: 10.1038/s41598-022-18884-4

    Figure Lengend Snippet: MF sprouting analysis by synaptoporin puncta density in lentiviral-infected DG

    Article Snippet: Sections were then incubated overnight at 4 °C with the following primary antibodies in blocking solution: rat anti-BrdU (1:200; AbD Serotec, Cat#OBT0030G, RRID: AB_609567), rabbit anti-GFAP (1:1000; Milipore, Cat#AB5804, RRID:AB_305124) and mouse anti-NeuN (1:400; Milipore, Cat#MAB377, RRID:AB_2298772) or rabbit anti-synaptoporin (1:500, Synaptic Systems, Cat#102002, RRID: AB_312851).

    Techniques: Infection