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    Cell Signaling Technology Inc mouse anti src mab3 l4a1
    (A) Domain structure of human <t>c-Src</t> protein (Uniprot: P12931) with key regulatory sites. From the N (amino) to the C (carboxyl) terminus, domains include the N-terminal SRC homology SH4 with the unique (U) (brown), SH3 (blue), SH2 (orange), kinase domain (KD; SH1) (green), and C-terminal regulatory (R) (red) domains. Domain range is indicated with residue numbers, shown below each domain (above for SH3). (B) Schematic representation of C-terminal c-Src truncated constructs (untagged) used in (C). Red asterisks: proteins detected in conditioned media (CMs). (C) Transiently transfected HEK293 cell extracts and CMs. c-Src expression and secretion were evaluated by western blot using an anti-N-term-c-Src (N-term, N-terminal) antibody <t>(mAb3)</t> (see ). (D) Schematic representation of N-term c-Src truncations from (E). Red asterisks: proteins detected in CMs. (E) Transiently transfected HEK293 cell extracts and CMs. c-Src expression and secretion were evaluated by western blot using anti-C-term-c-Src antibody (mAb4). (F) HEK293 cells were transiently transfected with c-Src. c-Src phosphorylation was determined by immunoblotting with anti-phos-Y419-Src or anti-phos-Y530-Src. (G) Cell extracts and CMs from transiently transfected HEK293 cells. c-Src expression and secretion were evaluated by western blot using anti-C-term-c-Src antibody. c-Src phosphorylation was determined by immunoblotting with anti-phos-Y419-Src antibody. (H) Schematic diagram displaying how c-Src truncations impact on its secretion. Arrows indicate secretion. Red arrow indicates secretion is hindered. MW, molecular weight; WT, wild type; EV, empty vector; Ab, antibody. Empty vector (EV) was used as a control. GAPDH and Coomassie blue staining were used in all immunoblots as loading controls. See also .
    Mouse Anti Src Mab3 L4a1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti src mab3 l4a1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    mouse anti src mab3 l4a1 - by Bioz Stars, 2024-05
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    (A) Domain structure of human c-Src protein (Uniprot: P12931) with key regulatory sites. From the N (amino) to the C (carboxyl) terminus, domains include the N-terminal SRC homology SH4 with the unique (U) (brown), SH3 (blue), SH2 (orange), kinase domain (KD; SH1) (green), and C-terminal regulatory (R) (red) domains. Domain range is indicated with residue numbers, shown below each domain (above for SH3). (B) Schematic representation of C-terminal c-Src truncated constructs (untagged) used in (C). Red asterisks: proteins detected in conditioned media (CMs). (C) Transiently transfected HEK293 cell extracts and CMs. c-Src expression and secretion were evaluated by western blot using an anti-N-term-c-Src (N-term, N-terminal) antibody (mAb3) (see ). (D) Schematic representation of N-term c-Src truncations from (E). Red asterisks: proteins detected in CMs. (E) Transiently transfected HEK293 cell extracts and CMs. c-Src expression and secretion were evaluated by western blot using anti-C-term-c-Src antibody (mAb4). (F) HEK293 cells were transiently transfected with c-Src. c-Src phosphorylation was determined by immunoblotting with anti-phos-Y419-Src or anti-phos-Y530-Src. (G) Cell extracts and CMs from transiently transfected HEK293 cells. c-Src expression and secretion were evaluated by western blot using anti-C-term-c-Src antibody. c-Src phosphorylation was determined by immunoblotting with anti-phos-Y419-Src antibody. (H) Schematic diagram displaying how c-Src truncations impact on its secretion. Arrows indicate secretion. Red arrow indicates secretion is hindered. MW, molecular weight; WT, wild type; EV, empty vector; Ab, antibody. Empty vector (EV) was used as a control. GAPDH and Coomassie blue staining were used in all immunoblots as loading controls. See also .

    Journal: Cell reports

    Article Title: PhosY-secretome profiling combined with kinase-substrate interaction screening defines active c-Src-driven extracellular signaling

    doi: 10.1016/j.celrep.2023.112539

    Figure Lengend Snippet: (A) Domain structure of human c-Src protein (Uniprot: P12931) with key regulatory sites. From the N (amino) to the C (carboxyl) terminus, domains include the N-terminal SRC homology SH4 with the unique (U) (brown), SH3 (blue), SH2 (orange), kinase domain (KD; SH1) (green), and C-terminal regulatory (R) (red) domains. Domain range is indicated with residue numbers, shown below each domain (above for SH3). (B) Schematic representation of C-terminal c-Src truncated constructs (untagged) used in (C). Red asterisks: proteins detected in conditioned media (CMs). (C) Transiently transfected HEK293 cell extracts and CMs. c-Src expression and secretion were evaluated by western blot using an anti-N-term-c-Src (N-term, N-terminal) antibody (mAb3) (see ). (D) Schematic representation of N-term c-Src truncations from (E). Red asterisks: proteins detected in CMs. (E) Transiently transfected HEK293 cell extracts and CMs. c-Src expression and secretion were evaluated by western blot using anti-C-term-c-Src antibody (mAb4). (F) HEK293 cells were transiently transfected with c-Src. c-Src phosphorylation was determined by immunoblotting with anti-phos-Y419-Src or anti-phos-Y530-Src. (G) Cell extracts and CMs from transiently transfected HEK293 cells. c-Src expression and secretion were evaluated by western blot using anti-C-term-c-Src antibody. c-Src phosphorylation was determined by immunoblotting with anti-phos-Y419-Src antibody. (H) Schematic diagram displaying how c-Src truncations impact on its secretion. Arrows indicate secretion. Red arrow indicates secretion is hindered. MW, molecular weight; WT, wild type; EV, empty vector; Ab, antibody. Empty vector (EV) was used as a control. GAPDH and Coomassie blue staining were used in all immunoblots as loading controls. See also .

    Article Snippet: Mouse anti-Src mAb3 (L4A1) , Cell Signaling Technology , Cat#2110; RRID:AB_10691385.

    Techniques: Residue, Construct, Transfection, Expressing, Western Blot, Molecular Weight, Plasmid Preparation, Staining

    Journal: Cell reports

    Article Title: PhosY-secretome profiling combined with kinase-substrate interaction screening defines active c-Src-driven extracellular signaling

    doi: 10.1016/j.celrep.2023.112539

    Figure Lengend Snippet:

    Article Snippet: Mouse anti-Src mAb3 (L4A1) , Cell Signaling Technology , Cat#2110; RRID:AB_10691385.

    Techniques: Recombinant, Virus, Bradford Assay, Staining, Colorimetric Assay, Software