anti shank3 antibody Search Results


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  • 94
    Santa Cruz Biotechnology anti shank3
    Acute knockdown of Shank family proteins via lentivirus-mediated shRNA expression. A , Schematics of Shank proteins indicating shRNA target sites. B , Schematics of lentiviral constructs used to introduce shRNAs into cells. Constructs contained one of four shRNAs targeting Shank1 (shShank1), Shank2 (shShank2), <t>Shank3</t> (shShank3), or Shank1 and Shank3 simultaneously (shShank13), as well as one of two fluorescent proteins (GFP, green fluorescent protein; tdT, tdTomato). pCMV, cytomegalovirus promoter; LTR, long terminal repeats; HIV-flap, a nuclear import sequence; pH1, constitutive H1 promoter; pUb, constitutive ubiquitin promoter; WRE, woodchuck hepatitis virus post-transcriptional regulatory element. C , D , Example ( C ) and quantification ( D ) of Western blotting for Shank protein levels in dissociated cortical neuron culture. Actin was used as a loading control. GFP and tdT refer to cultures infected with virus constructs containing the fluorescent protein and no shRNA. shShank13 + 2, knockdown of all three Shank members by superinfection of shShank13 and shShank2. E , A Western blotting for Shank3 levels in HEK cells cotransfected with a rat Shank3 expression vector and a GFP or shRNA expressing construct as indicated. One-way ANOVA was used for each quantification, followed by Tukey’s test, * p
    Anti Shank3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti shank3/product/Santa Cruz Biotechnology
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti shank3 - by Bioz Stars, 2022-11
    94/100 stars
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    88
    NeuroMab anti shank3
    Acute knockdown of Shank family proteins via lentivirus-mediated shRNA expression. A , Schematics of Shank proteins indicating shRNA target sites. B , Schematics of lentiviral constructs used to introduce shRNAs into cells. Constructs contained one of four shRNAs targeting Shank1 (shShank1), Shank2 (shShank2), <t>Shank3</t> (shShank3), or Shank1 and Shank3 simultaneously (shShank13), as well as one of two fluorescent proteins (GFP, green fluorescent protein; tdT, tdTomato). pCMV, cytomegalovirus promoter; LTR, long terminal repeats; HIV-flap, a nuclear import sequence; pH1, constitutive H1 promoter; pUb, constitutive ubiquitin promoter; WRE, woodchuck hepatitis virus post-transcriptional regulatory element. C , D , Example ( C ) and quantification ( D ) of Western blotting for Shank protein levels in dissociated cortical neuron culture. Actin was used as a loading control. GFP and tdT refer to cultures infected with virus constructs containing the fluorescent protein and no shRNA. shShank13 + 2, knockdown of all three Shank members by superinfection of shShank13 and shShank2. E , A Western blotting for Shank3 levels in HEK cells cotransfected with a rat Shank3 expression vector and a GFP or shRNA expressing construct as indicated. One-way ANOVA was used for each quantification, followed by Tukey’s test, * p
    Anti Shank3, supplied by NeuroMab, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti shank3/product/NeuroMab
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti shank3 - by Bioz Stars, 2022-11
    88/100 stars
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    94
    Abcam anti shank3
    Shank protein analysis in the male and female mouse cortex. Levels of Shank1, Shank2 and <t>Shank3</t> protein in the frontal cortex of male and female at E17.5 (A) and P7.5 (B) ; ( n = 4 of each sex), * P
    Anti Shank3, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti shank3/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti shank3 - by Bioz Stars, 2022-11
    94/100 stars
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    80
    NeuroMab mouse anti shank3
    Synaptic transport of proteins to PSD is altered in Flr mice. WT FLR and Flr hippocampal synaptosome extracts were used for quantitative Western blot analyses of synaptic proteins. A1 , A2 , Levels of full-length MyoVa and TLS/FUS were reduced in Flr extracts. B1 , B2 , Expression levels of major MAGUKs (PSD-95, PSD-93, SAP102) and ( C1 , C2 ) SAPAP1, SHANK1, <t>SHANK3,</t> Homer1b/c, and IP3R were significantly reduced in Flr. D1 , D2 , mGluRs, NMDAR, AMPAR subunit levels, and FMRP were not changed in Flr when compared with WT FLR . Homogenate (Homo) and synaptosome (Syn) was prepared from whole hippocampus. Protein levels in Flr were quantified and plotted as % of WT FLR ; n = 3 samples per group/each sample pool three to five animals. Unpaired t test was used for statistical analysis. Error bars represent ±SEM; * p ≤ 0.05, ** p ≤ 0.001.
    Mouse Anti Shank3, supplied by NeuroMab, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti shank3/product/NeuroMab
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    Image Search Results


    Acute knockdown of Shank family proteins via lentivirus-mediated shRNA expression. A , Schematics of Shank proteins indicating shRNA target sites. B , Schematics of lentiviral constructs used to introduce shRNAs into cells. Constructs contained one of four shRNAs targeting Shank1 (shShank1), Shank2 (shShank2), Shank3 (shShank3), or Shank1 and Shank3 simultaneously (shShank13), as well as one of two fluorescent proteins (GFP, green fluorescent protein; tdT, tdTomato). pCMV, cytomegalovirus promoter; LTR, long terminal repeats; HIV-flap, a nuclear import sequence; pH1, constitutive H1 promoter; pUb, constitutive ubiquitin promoter; WRE, woodchuck hepatitis virus post-transcriptional regulatory element. C , D , Example ( C ) and quantification ( D ) of Western blotting for Shank protein levels in dissociated cortical neuron culture. Actin was used as a loading control. GFP and tdT refer to cultures infected with virus constructs containing the fluorescent protein and no shRNA. shShank13 + 2, knockdown of all three Shank members by superinfection of shShank13 and shShank2. E , A Western blotting for Shank3 levels in HEK cells cotransfected with a rat Shank3 expression vector and a GFP or shRNA expressing construct as indicated. One-way ANOVA was used for each quantification, followed by Tukey’s test, * p

    Journal: eNeuro

    Article Title: Shank Proteins Differentially Regulate Synaptic Transmission

    doi: 10.1523/ENEURO.0163-15.2017

    Figure Lengend Snippet: Acute knockdown of Shank family proteins via lentivirus-mediated shRNA expression. A , Schematics of Shank proteins indicating shRNA target sites. B , Schematics of lentiviral constructs used to introduce shRNAs into cells. Constructs contained one of four shRNAs targeting Shank1 (shShank1), Shank2 (shShank2), Shank3 (shShank3), or Shank1 and Shank3 simultaneously (shShank13), as well as one of two fluorescent proteins (GFP, green fluorescent protein; tdT, tdTomato). pCMV, cytomegalovirus promoter; LTR, long terminal repeats; HIV-flap, a nuclear import sequence; pH1, constitutive H1 promoter; pUb, constitutive ubiquitin promoter; WRE, woodchuck hepatitis virus post-transcriptional regulatory element. C , D , Example ( C ) and quantification ( D ) of Western blotting for Shank protein levels in dissociated cortical neuron culture. Actin was used as a loading control. GFP and tdT refer to cultures infected with virus constructs containing the fluorescent protein and no shRNA. shShank13 + 2, knockdown of all three Shank members by superinfection of shShank13 and shShank2. E , A Western blotting for Shank3 levels in HEK cells cotransfected with a rat Shank3 expression vector and a GFP or shRNA expressing construct as indicated. One-way ANOVA was used for each quantification, followed by Tukey’s test, * p

    Article Snippet: Western blotting The following primary antibodies were used: anti-Shank1 (1:200, Abcam, catalog #ab154224), anti-Shank2 (1:100, Cell Signaling, catalog #12218), anti-Shank3 (1:400, Santa Cruz, RRID: AB_2301759 ), anti-PanShank (1:1000, Neuromab, RRID: AB_10674115 ), and anti-actin (1:3000, Sigma, RRID: AB_476697 ).

    Techniques: shRNA, Expressing, Construct, Introduce, Sequencing, Western Blot, Infection, Plasmid Preparation

    The SAM domain was required for Shank2 to maintain AMPAR eEPSCs. A , Schematics of the domain structure of Shank2 and Shank2ΔSAM in the replacement construct. Silent mutations in Shank2 were indicated with *. B , Examples of Western blotting for Shank2 and Shank3 protein levels in the total cell homogenate and the synaptoneurosome fraction from dissociated neuronal cultures infected with GFP, Shank2 replacement (Shank2RP), and Shank2ΔSAM replacement (ΔSAMRP). C , D , Comparison of uninfected (control) and infected [Shank2RP ( C ); ΔSAMRP ( D )] neuronal responses measured by AMPAR eEPSCs (Shank2RP, individual data point, mean; ΔSAMRP, individual data point, ● mean).

    Journal: eNeuro

    Article Title: Shank Proteins Differentially Regulate Synaptic Transmission

    doi: 10.1523/ENEURO.0163-15.2017

    Figure Lengend Snippet: The SAM domain was required for Shank2 to maintain AMPAR eEPSCs. A , Schematics of the domain structure of Shank2 and Shank2ΔSAM in the replacement construct. Silent mutations in Shank2 were indicated with *. B , Examples of Western blotting for Shank2 and Shank3 protein levels in the total cell homogenate and the synaptoneurosome fraction from dissociated neuronal cultures infected with GFP, Shank2 replacement (Shank2RP), and Shank2ΔSAM replacement (ΔSAMRP). C , D , Comparison of uninfected (control) and infected [Shank2RP ( C ); ΔSAMRP ( D )] neuronal responses measured by AMPAR eEPSCs (Shank2RP, individual data point, mean; ΔSAMRP, individual data point, ● mean).

    Article Snippet: Western blotting The following primary antibodies were used: anti-Shank1 (1:200, Abcam, catalog #ab154224), anti-Shank2 (1:100, Cell Signaling, catalog #12218), anti-Shank3 (1:400, Santa Cruz, RRID: AB_2301759 ), anti-PanShank (1:1000, Neuromab, RRID: AB_10674115 ), and anti-actin (1:3000, Sigma, RRID: AB_476697 ).

    Techniques: Construct, Western Blot, Infection

    Simultaneous knockdown of Shank1 and Shank2 decreased both AMPAR- and NMDAR-mediated currents. A , B , Comparison of uninfected (control) and infected ( A , shShank13 + 2; B , shShank1 + 2) neuronal responses measured by eEPSCs [AMPAR eEPSCs ( Aa , Ba , left panels); NMDAR eEPSCs ( Ab , Bb , middle panels), and mEPSCs ( Ac , right panel)]. C , Summary of effects on AMPAR EPSCs (left panel) and NMDAR EPSCs (right panel) of knockdown of Shank1, Shank2, Shank3, Shank13 + 2, Shank1 + 2, mean ± SEM.

    Journal: eNeuro

    Article Title: Shank Proteins Differentially Regulate Synaptic Transmission

    doi: 10.1523/ENEURO.0163-15.2017

    Figure Lengend Snippet: Simultaneous knockdown of Shank1 and Shank2 decreased both AMPAR- and NMDAR-mediated currents. A , B , Comparison of uninfected (control) and infected ( A , shShank13 + 2; B , shShank1 + 2) neuronal responses measured by eEPSCs [AMPAR eEPSCs ( Aa , Ba , left panels); NMDAR eEPSCs ( Ab , Bb , middle panels), and mEPSCs ( Ac , right panel)]. C , Summary of effects on AMPAR EPSCs (left panel) and NMDAR EPSCs (right panel) of knockdown of Shank1, Shank2, Shank3, Shank13 + 2, Shank1 + 2, mean ± SEM.

    Article Snippet: Western blotting The following primary antibodies were used: anti-Shank1 (1:200, Abcam, catalog #ab154224), anti-Shank2 (1:100, Cell Signaling, catalog #12218), anti-Shank3 (1:400, Santa Cruz, RRID: AB_2301759 ), anti-PanShank (1:1000, Neuromab, RRID: AB_10674115 ), and anti-actin (1:3000, Sigma, RRID: AB_476697 ).

    Techniques: Infection

    Shank3c rescues synaptic deficit caused by knocking down Shank1 or Shank2. A , Schematics of the domain structure of Shank3c in the replacement construct. B , Examples of Western blotting for Shank2 and Shank3 protein levels in the total cell homogenate and the synaptoneurosome fraction from dissociated neuronal cultures infected with GFP, shShank13, Shank3c replacement (Shank3RP). C , Examples of Western blotting for Shank2 and Shank3 protein levels in the total cell homogenate and the synaptoneurosome fraction from dissociated neuronal cultures infected with GFP, shShank2, Shank3c replacement (Shank3RP). D , Comparison of uninfected (control) and infected (shShank13 Shank3RP) neuronal responses measured by AMPAR eEPSCs ( individual data point, mean). E , Comparison of uninfected (control) and infected (shShank2 Shank3RP) neuronal responses measured by AMPAR eEPSCs ( individual data point, mean).

    Journal: eNeuro

    Article Title: Shank Proteins Differentially Regulate Synaptic Transmission

    doi: 10.1523/ENEURO.0163-15.2017

    Figure Lengend Snippet: Shank3c rescues synaptic deficit caused by knocking down Shank1 or Shank2. A , Schematics of the domain structure of Shank3c in the replacement construct. B , Examples of Western blotting for Shank2 and Shank3 protein levels in the total cell homogenate and the synaptoneurosome fraction from dissociated neuronal cultures infected with GFP, shShank13, Shank3c replacement (Shank3RP). C , Examples of Western blotting for Shank2 and Shank3 protein levels in the total cell homogenate and the synaptoneurosome fraction from dissociated neuronal cultures infected with GFP, shShank2, Shank3c replacement (Shank3RP). D , Comparison of uninfected (control) and infected (shShank13 Shank3RP) neuronal responses measured by AMPAR eEPSCs ( individual data point, mean). E , Comparison of uninfected (control) and infected (shShank2 Shank3RP) neuronal responses measured by AMPAR eEPSCs ( individual data point, mean).

    Article Snippet: Western blotting The following primary antibodies were used: anti-Shank1 (1:200, Abcam, catalog #ab154224), anti-Shank2 (1:100, Cell Signaling, catalog #12218), anti-Shank3 (1:400, Santa Cruz, RRID: AB_2301759 ), anti-PanShank (1:1000, Neuromab, RRID: AB_10674115 ), and anti-actin (1:3000, Sigma, RRID: AB_476697 ).

    Techniques: Construct, Western Blot, Infection

    Shank protein analysis in the male and female mouse cortex. Levels of Shank1, Shank2 and Shank3 protein in the frontal cortex of male and female at E17.5 (A) and P7.5 (B) ; ( n = 4 of each sex), * P

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Sex Hormones Regulate SHANK Expression

    doi: 10.3389/fnmol.2018.00337

    Figure Lengend Snippet: Shank protein analysis in the male and female mouse cortex. Levels of Shank1, Shank2 and Shank3 protein in the frontal cortex of male and female at E17.5 (A) and P7.5 (B) ; ( n = 4 of each sex), * P

    Article Snippet: PVDF membranes were probed with mouse anti-pan-SHANK (1:500; Neuromab), mouse monoclonal β3-tubulin (1:20,000; Promega-G7121), anti-SHANK1 (Synaptic Systems, polyclonal rabbit purified antibody, 1:500 dilution), anti-SHANK2 (Synaptic Systems, polyclonal guinea pig antiserum, 1:500 dilution), anti-SHANK3 (ab140030, Abcam, 1:1,000 dilution).

    Techniques:

    Synaptic transport of proteins to PSD is altered in Flr mice. WT FLR and Flr hippocampal synaptosome extracts were used for quantitative Western blot analyses of synaptic proteins. A1 , A2 , Levels of full-length MyoVa and TLS/FUS were reduced in Flr extracts. B1 , B2 , Expression levels of major MAGUKs (PSD-95, PSD-93, SAP102) and ( C1 , C2 ) SAPAP1, SHANK1, SHANK3, Homer1b/c, and IP3R were significantly reduced in Flr. D1 , D2 , mGluRs, NMDAR, AMPAR subunit levels, and FMRP were not changed in Flr when compared with WT FLR . Homogenate (Homo) and synaptosome (Syn) was prepared from whole hippocampus. Protein levels in Flr were quantified and plotted as % of WT FLR ; n = 3 samples per group/each sample pool three to five animals. Unpaired t test was used for statistical analysis. Error bars represent ±SEM; * p ≤ 0.05, ** p ≤ 0.001.

    Journal: eNeuro

    Article Title: Myosin Va Brain-Specific Mutation Alters Mouse Behavior and Disrupts Hippocampal Synapses

    doi: 10.1523/ENEURO.0284-20.2020

    Figure Lengend Snippet: Synaptic transport of proteins to PSD is altered in Flr mice. WT FLR and Flr hippocampal synaptosome extracts were used for quantitative Western blot analyses of synaptic proteins. A1 , A2 , Levels of full-length MyoVa and TLS/FUS were reduced in Flr extracts. B1 , B2 , Expression levels of major MAGUKs (PSD-95, PSD-93, SAP102) and ( C1 , C2 ) SAPAP1, SHANK1, SHANK3, Homer1b/c, and IP3R were significantly reduced in Flr. D1 , D2 , mGluRs, NMDAR, AMPAR subunit levels, and FMRP were not changed in Flr when compared with WT FLR . Homogenate (Homo) and synaptosome (Syn) was prepared from whole hippocampus. Protein levels in Flr were quantified and plotted as % of WT FLR ; n = 3 samples per group/each sample pool three to five animals. Unpaired t test was used for statistical analysis. Error bars represent ±SEM; * p ≤ 0.05, ** p ≤ 0.001.

    Article Snippet: Primary antibodies used were: rabbit anti-MyoVa (Sigma, LE18), mouse anti-TLS (Santa Cruz, sc-47 711), rabbit anti-mGluR1 (Millipore, 07-617), rabbit anti-mGluR5 (Neuromics, RA16100), mouse anti-fragile X mental retardation protein (FMRP; Millipore, 1C3), mouse anti-PSD-95 (NeuroMab, K28/43), mouse anti-PSD-93 (NeuroMab, N18/30), mouse anti-SAP102 (NeuroMab, N19/2), mouse anti-SAPAP1 (NeuroMab, N238/29), mouse anti-Shank1 (NeuroMab, N22/21), mouse anti-Shank3 (NeuroMab, N367/62), mouse anti-GluN1 (NeuroMab, N308-48), rabbit anti-GluN2A (Millipore, A12W), mouse anti-GluN2B (NeuroMab N59/20 and N59/36), mouse anti-GluA2 (NeuroMab, L21/32), mouse anti-homer 1 (NeuroMab, L113/130), mouse anti-IP3R (NeuroMab, N18/30), rabbit anti-tubulin β III (Abcam, ab6046), and mouse anti-actin (Sigma, AC-74).

    Techniques: Mouse Assay, Western Blot, Expressing