anti rxrα d 20 Santa Cruz Biotechnology Search Results


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    Santa Cruz Biotechnology anti rxrα
    CTBP2 is a transcriptional cofactor for RXR α/ RAR α. (A to C) Activation of a RARE-luciferase (RARE-Luc) reporter gene by <t>RXRα/RARα</t> requires CTBP2. The normalized luciferase activities shown represent ratios between luciferase
    Anti Rxrα, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 89/100, based on 211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology rxrα d 20
    CTBP2 is a transcriptional cofactor for RXR α/ RAR α. (A to C) Activation of a RARE-luciferase (RARE-Luc) reporter gene by <t>RXRα/RARα</t> requires CTBP2. The normalized luciferase activities shown represent ratios between luciferase
    Rxrα D 20, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti retinoid x receptor α rxrα antibody
    CTBP2 is a transcriptional cofactor for RXR α/ RAR α. (A to C) Activation of a RARE-luciferase (RARE-Luc) reporter gene by <t>RXRα/RARα</t> requires CTBP2. The normalized luciferase activities shown represent ratios between luciferase
    Anti Retinoid X Receptor α Rxrα Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology polyclonal anti rxrα antibody
    CTBP2 is a transcriptional cofactor for RXR α/ RAR α. (A to C) Activation of a RARE-luciferase (RARE-Luc) reporter gene by <t>RXRα/RARα</t> requires CTBP2. The normalized luciferase activities shown represent ratios between luciferase
    Polyclonal Anti Rxrα Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology chip quality anti rxrα antibody
    Correlation of gender differential <t>RXRα,</t> Pol2 and RNA expression levels. A) Correlation of RXRα and Pol2 binding with gene expression levels. B) Scatterplot representation of gender differential RXRα binding with gender differential gene expression on the left-hand side, and scatterplot representation of gender differential Pol2 binding with gender differential gene expression on the right-hand side. C) Genes with a gender specific positive correlation between RXRα binding Pol2 binding and changes in RNA levels (see also Fig. S4A–B and Table S6A–H in File S1 ).
    Chip Quality Anti Rxrα Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti human rxrα
    <t>RXRα</t> is required for R -etodolac-induced apoptosis. ( a ) Inhibition of RXRα expression by RXRα siRNA. LNCaP cells were untransfected or transfected with a pool of RXRα siRNAs or control GFP siRNA for 48 h. Cell lysates were
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    Santa Cruz Biotechnology anti human rxrα d 20 antibodies
    <t>RXRα</t> is required for R -etodolac-induced apoptosis. ( a ) Inhibition of RXRα expression by RXRα siRNA. LNCaP cells were untransfected or transfected with a pool of RXRα siRNAs or control GFP siRNA for 48 h. Cell lysates were
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    Santa Cruz Biotechnology rabbit anti rxrα antibody
    The heterodimerization of hPXR S350D and <t>RXRα</t> is impaired
    Rabbit Anti Rxrα Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology rabbit polyclonal anti rxrα antibodies
    The heterodimerization of hPXR S350D and <t>RXRα</t> is impaired
    Rabbit Polyclonal Anti Rxrα Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    CTBP2 is a transcriptional cofactor for RXR α/ RAR α. (A to C) Activation of a RARE-luciferase (RARE-Luc) reporter gene by RXRα/RARα requires CTBP2. The normalized luciferase activities shown represent ratios between luciferase

    Journal: Molecular and Cellular Biology

    Article Title: The Corepressor CTBP2 Is a Coactivator of Retinoic Acid Receptor/Retinoid X Receptor in Retinoic Acid Signaling

    doi: 10.1128/MCB.01213-12

    Figure Lengend Snippet: CTBP2 is a transcriptional cofactor for RXR α/ RAR α. (A to C) Activation of a RARE-luciferase (RARE-Luc) reporter gene by RXRα/RARα requires CTBP2. The normalized luciferase activities shown represent ratios between luciferase

    Article Snippet: The following antibodies were used: anti-p300 (C-20, Sc585; Santa Cruz), histone H3 (ab1791; Abcam) and H3 acetyl antibodies (06-599; Upstate Millipore), normal rabbit IgG (Santa Cruz), anti-RXRα (D-20; Santa Cruz), anti-CTBP2 (612044; BD Transduction Laboratories), and anti-CD11b-fluorescein isothiocyanate (FITC) antibody (F2648; Sigma). siRNA for mouse Ctbp2 was purchased from Thermo Scientific (#MU-059787-01-0002; SiGenome mouse Ctbp2 ).

    Techniques: Activation Assay, Luciferase

    CTBP2 physically associates with RARα/RXRα and the RARE region of RA target gene promoters. (A) In vitro interaction of CTBP2 and RXRα. A GST pulldown assay was used to determine the binding between GST fusions of RARα

    Journal: Molecular and Cellular Biology

    Article Title: The Corepressor CTBP2 Is a Coactivator of Retinoic Acid Receptor/Retinoid X Receptor in Retinoic Acid Signaling

    doi: 10.1128/MCB.01213-12

    Figure Lengend Snippet: CTBP2 physically associates with RARα/RXRα and the RARE region of RA target gene promoters. (A) In vitro interaction of CTBP2 and RXRα. A GST pulldown assay was used to determine the binding between GST fusions of RARα

    Article Snippet: The following antibodies were used: anti-p300 (C-20, Sc585; Santa Cruz), histone H3 (ab1791; Abcam) and H3 acetyl antibodies (06-599; Upstate Millipore), normal rabbit IgG (Santa Cruz), anti-RXRα (D-20; Santa Cruz), anti-CTBP2 (612044; BD Transduction Laboratories), and anti-CD11b-fluorescein isothiocyanate (FITC) antibody (F2648; Sigma). siRNA for mouse Ctbp2 was purchased from Thermo Scientific (#MU-059787-01-0002; SiGenome mouse Ctbp2 ).

    Techniques: In Vitro, GST Pulldown Assay, Binding Assay

    Direct protein-protein interactions between NPDC-1 and RXR. Bacterially expressed flag epitope tagged NPDC-1 [NPDC(F)] (40 μg) was incubated with glutathione-agarose bound GST/human RXRα (GST-RXR) fusion protein in the absence or presence of bacterially expressed human RARγ (RAR) (60 μg) or in the presence of 9-cis retinoic acid (9cRA) (10 -6 M) as indicated. NPDC(F) was also incubated with glutathione-agarose bound GST. After extensive washing, the retained proteins were resolved by gel electrophoresis and analyzed by immunoblot with anti-flag M2 antibodies. One percent (0.4 μg) of the available NPDC(F) input was also electrophoresed and a unique immunoreactive band of approximately 45 kDa representing NPDC(F) is indicated by an arrow. The position of prestained molecular weight standards are shown.

    Journal: Nuclear Receptor

    Article Title: A neuronal-specific differentiation protein that directly modulates retinoid receptor transcriptional activation

    doi: 10.1186/1478-1336-1-7

    Figure Lengend Snippet: Direct protein-protein interactions between NPDC-1 and RXR. Bacterially expressed flag epitope tagged NPDC-1 [NPDC(F)] (40 μg) was incubated with glutathione-agarose bound GST/human RXRα (GST-RXR) fusion protein in the absence or presence of bacterially expressed human RARγ (RAR) (60 μg) or in the presence of 9-cis retinoic acid (9cRA) (10 -6 M) as indicated. NPDC(F) was also incubated with glutathione-agarose bound GST. After extensive washing, the retained proteins were resolved by gel electrophoresis and analyzed by immunoblot with anti-flag M2 antibodies. One percent (0.4 μg) of the available NPDC(F) input was also electrophoresed and a unique immunoreactive band of approximately 45 kDa representing NPDC(F) is indicated by an arrow. The position of prestained molecular weight standards are shown.

    Article Snippet: In order to identify proteins present in complexes, supershifts with antibodies to RXRα (D-20, Santa Cruz) and NPDC-1 (custom antibody and α-thioredoxin, Thio-probe, Santa Cruz) were used with the above reactions.

    Techniques: FLAG-tag, Incubation, Nucleic Acid Electrophoresis, Molecular Weight

    RXR binds the amino terminus of hNPDC-1 . Approximately 10 μg of recombinant S-tagged hNPDC-1 or NPDC-1 deletion mutant (A) was pre-coupled to S-protein to generate a 50% slurry of NPDC-1-beads. Reactions containing 30 μl NPDC-beads, 5 μg of recombinant GST-RXRα and 300 μl of 1X gel-shift buffer were incubated at 4°C for 1 hour with rotation. S-protein pellets were collected by centrifugation and washed 3 times in gel-shift buffer. Proteins were released from the pellet by resuspending S-protein agarose in 2X SDS-PAGE sample buffer. The amount of GST-RXRα bound to beads was analyzed by Western blotting onto nitrocellulose filters and probing blots with anti-RXRα (B, top panel). To normalize for unequal expression of the various deletion mutants, duplicate reactions without GST-RXRα were performed and subjected to coomassie staining instead of immunoblotting, and NIH Image Software was used to normalize the bands in the immunoblot (B, top panel) to the amount of S-tagged NPDC-1 precipitated in the coomassie stained gel. Normalized binding is depicted in B, bottom panel.

    Journal: Nuclear Receptor

    Article Title: A neuronal-specific differentiation protein that directly modulates retinoid receptor transcriptional activation

    doi: 10.1186/1478-1336-1-7

    Figure Lengend Snippet: RXR binds the amino terminus of hNPDC-1 . Approximately 10 μg of recombinant S-tagged hNPDC-1 or NPDC-1 deletion mutant (A) was pre-coupled to S-protein to generate a 50% slurry of NPDC-1-beads. Reactions containing 30 μl NPDC-beads, 5 μg of recombinant GST-RXRα and 300 μl of 1X gel-shift buffer were incubated at 4°C for 1 hour with rotation. S-protein pellets were collected by centrifugation and washed 3 times in gel-shift buffer. Proteins were released from the pellet by resuspending S-protein agarose in 2X SDS-PAGE sample buffer. The amount of GST-RXRα bound to beads was analyzed by Western blotting onto nitrocellulose filters and probing blots with anti-RXRα (B, top panel). To normalize for unequal expression of the various deletion mutants, duplicate reactions without GST-RXRα were performed and subjected to coomassie staining instead of immunoblotting, and NIH Image Software was used to normalize the bands in the immunoblot (B, top panel) to the amount of S-tagged NPDC-1 precipitated in the coomassie stained gel. Normalized binding is depicted in B, bottom panel.

    Article Snippet: In order to identify proteins present in complexes, supershifts with antibodies to RXRα (D-20, Santa Cruz) and NPDC-1 (custom antibody and α-thioredoxin, Thio-probe, Santa Cruz) were used with the above reactions.

    Techniques: Recombinant, Mutagenesis, Electrophoretic Mobility Shift Assay, Incubation, Centrifugation, SDS Page, Western Blot, Expressing, Staining, Software, Binding Assay

    NPDC-1 Alters RARβ••••α and RXRα DNA binding properties. Oligonucleotides corresponding to the consensus RARβ•RARE (DR-5: A) or the consensus RXRα RARE (DR-1: B C) were end-labeled with 32- P by T4 polynucleotide kinase according to the manufacture's instructions. The resultant DNA binding probes were incubated with PC12 lysate (A), recombinant NPDC-1 and recombinant RXRα (B C) as indicated. DNA:protein complexes were resolved on non-denaturing PAGE gels which were subsequently exposed to Kodak Xar-5 film. In C, the presence of NPDC-1 within the RXR gel-shift complex was assayed by supershift. Antibodies specific for either NPDC-1 or RXR were used to identify proteins present in the complex.

    Journal: Nuclear Receptor

    Article Title: A neuronal-specific differentiation protein that directly modulates retinoid receptor transcriptional activation

    doi: 10.1186/1478-1336-1-7

    Figure Lengend Snippet: NPDC-1 Alters RARβ••••α and RXRα DNA binding properties. Oligonucleotides corresponding to the consensus RARβ•RARE (DR-5: A) or the consensus RXRα RARE (DR-1: B C) were end-labeled with 32- P by T4 polynucleotide kinase according to the manufacture's instructions. The resultant DNA binding probes were incubated with PC12 lysate (A), recombinant NPDC-1 and recombinant RXRα (B C) as indicated. DNA:protein complexes were resolved on non-denaturing PAGE gels which were subsequently exposed to Kodak Xar-5 film. In C, the presence of NPDC-1 within the RXR gel-shift complex was assayed by supershift. Antibodies specific for either NPDC-1 or RXR were used to identify proteins present in the complex.

    Article Snippet: In order to identify proteins present in complexes, supershifts with antibodies to RXRα (D-20, Santa Cruz) and NPDC-1 (custom antibody and α-thioredoxin, Thio-probe, Santa Cruz) were used with the above reactions.

    Techniques: Binding Assay, Labeling, Incubation, Recombinant, Polyacrylamide Gel Electrophoresis, Electrophoretic Mobility Shift Assay

    RXRα interacts with human and mouse P1 promoters

    Journal: Placenta

    Article Title: RXR? and LXR activate two promoters in placenta- and tumor-specific expression of PLAC1

    doi: 10.1016/j.placenta.2011.08.011

    Figure Lengend Snippet: RXRα interacts with human and mouse P1 promoters

    Article Snippet: Chromatin immunoprecipitation was performed with anti-RXRα antibody (D-20, Santa Cruz, CA, USA) following the supplier's protocol.

    Techniques:

    Correlation of gender differential RXRα, Pol2 and RNA expression levels. A) Correlation of RXRα and Pol2 binding with gene expression levels. B) Scatterplot representation of gender differential RXRα binding with gender differential gene expression on the left-hand side, and scatterplot representation of gender differential Pol2 binding with gender differential gene expression on the right-hand side. C) Genes with a gender specific positive correlation between RXRα binding Pol2 binding and changes in RNA levels (see also Fig. S4A–B and Table S6A–H in File S1 ).

    Journal: PLoS ONE

    Article Title: Sexually Dimorphic Genome-Wide Binding of Retinoid X Receptor alpha (RXR?) Determines Male-Female Differences in the Expression of Hepatic Lipid Processing Genes in Mice

    doi: 10.1371/journal.pone.0071538

    Figure Lengend Snippet: Correlation of gender differential RXRα, Pol2 and RNA expression levels. A) Correlation of RXRα and Pol2 binding with gene expression levels. B) Scatterplot representation of gender differential RXRα binding with gender differential gene expression on the left-hand side, and scatterplot representation of gender differential Pol2 binding with gender differential gene expression on the right-hand side. C) Genes with a gender specific positive correlation between RXRα binding Pol2 binding and changes in RNA levels (see also Fig. S4A–B and Table S6A–H in File S1 ).

    Article Snippet: ChIP-quality anti-RXRα antibody (D-20,sc-774X, Santa Cruz Biotechnology, Santa Cruz, CA) and RNA Pol2 (ab24758, Abcam) were used.

    Techniques: RNA Expression, Binding Assay, Expressing

    Gender differential responsiveness to RXRα activation in vivo. Male and female mice were gavaged once daily for 5 days with the RXRα ligand LG268. A) Relative RNA levels in male and female mouse liver as determined by QPCR in response to RXRα activation by LG268 for selected male and female enriched genes. B) RXRα occupancy in response to RXRα activation on selected male and female enriched genes (see also Fig. S5 and S6 ). *p

    Journal: PLoS ONE

    Article Title: Sexually Dimorphic Genome-Wide Binding of Retinoid X Receptor alpha (RXR?) Determines Male-Female Differences in the Expression of Hepatic Lipid Processing Genes in Mice

    doi: 10.1371/journal.pone.0071538

    Figure Lengend Snippet: Gender differential responsiveness to RXRα activation in vivo. Male and female mice were gavaged once daily for 5 days with the RXRα ligand LG268. A) Relative RNA levels in male and female mouse liver as determined by QPCR in response to RXRα activation by LG268 for selected male and female enriched genes. B) RXRα occupancy in response to RXRα activation on selected male and female enriched genes (see also Fig. S5 and S6 ). *p

    Article Snippet: ChIP-quality anti-RXRα antibody (D-20,sc-774X, Santa Cruz Biotechnology, Santa Cruz, CA) and RNA Pol2 (ab24758, Abcam) were used.

    Techniques: Activation Assay, In Vivo, Mouse Assay, Real-time Polymerase Chain Reaction

    Genome wide mapping of RXRα and Pol2 binding sites in male and female mouse liver. A) Venn diagrams show number of RXRα binding sites in male and female livers with 37602 binding sites shared between genders, and 10243 unique RXRα male and 9275 unique RXRα female binding sites (see also Table S1A and S1B in File S1 ). B) Venn diagrams show number of Pol2 binding sites in male and female livers, with 19307 binding sites shared between genders, and 6037 unique male and 1332 unique female Pol2 binding sites. C) Venn diagrams representing number of genes associated with RXRα peaks shown in A), with 11660 genes shared between genders, and 1099 unique genes for male and 1021 genes for female. D) Western blot analysis shows increased nuclear RXRα protein levels in female mouse liver compared to male mouse liver. E) Number of RXRα peaks as stratified by peak height (see also Table S2A and S2B in File S1 ). F) Genomic positions of RXRα binding sites in male and female mouse liver. Data expressed as percentage of total peaks per gender. RXRα binding was significantly increased at TSS (+/−500 bp), TES, within exons and introns with a depletion in binding compared to the expected number of sites based on random distribution (see also Fig. S1A and S1B ).

    Journal: PLoS ONE

    Article Title: Sexually Dimorphic Genome-Wide Binding of Retinoid X Receptor alpha (RXR?) Determines Male-Female Differences in the Expression of Hepatic Lipid Processing Genes in Mice

    doi: 10.1371/journal.pone.0071538

    Figure Lengend Snippet: Genome wide mapping of RXRα and Pol2 binding sites in male and female mouse liver. A) Venn diagrams show number of RXRα binding sites in male and female livers with 37602 binding sites shared between genders, and 10243 unique RXRα male and 9275 unique RXRα female binding sites (see also Table S1A and S1B in File S1 ). B) Venn diagrams show number of Pol2 binding sites in male and female livers, with 19307 binding sites shared between genders, and 6037 unique male and 1332 unique female Pol2 binding sites. C) Venn diagrams representing number of genes associated with RXRα peaks shown in A), with 11660 genes shared between genders, and 1099 unique genes for male and 1021 genes for female. D) Western blot analysis shows increased nuclear RXRα protein levels in female mouse liver compared to male mouse liver. E) Number of RXRα peaks as stratified by peak height (see also Table S2A and S2B in File S1 ). F) Genomic positions of RXRα binding sites in male and female mouse liver. Data expressed as percentage of total peaks per gender. RXRα binding was significantly increased at TSS (+/−500 bp), TES, within exons and introns with a depletion in binding compared to the expected number of sites based on random distribution (see also Fig. S1A and S1B ).

    Article Snippet: ChIP-quality anti-RXRα antibody (D-20,sc-774X, Santa Cruz Biotechnology, Santa Cruz, CA) and RNA Pol2 (ab24758, Abcam) were used.

    Techniques: Genome Wide, Binding Assay, Western Blot

    RXRα and Pol2 binding sites in mouse liver 10 kB surrounding the Transcriptional Start Sites. A) Venn diagram showing number of genes with shared RXRα regulation between male and female within 10 kB up and downstream of TSS, with 1285 genes found in male only and 1152 regulated by RXRα in females (see also Table S3A–D in File S1 ). B) Ontology analysis and pathway analysis of RXRα peaks with score > 6 or

    Journal: PLoS ONE

    Article Title: Sexually Dimorphic Genome-Wide Binding of Retinoid X Receptor alpha (RXR?) Determines Male-Female Differences in the Expression of Hepatic Lipid Processing Genes in Mice

    doi: 10.1371/journal.pone.0071538

    Figure Lengend Snippet: RXRα and Pol2 binding sites in mouse liver 10 kB surrounding the Transcriptional Start Sites. A) Venn diagram showing number of genes with shared RXRα regulation between male and female within 10 kB up and downstream of TSS, with 1285 genes found in male only and 1152 regulated by RXRα in females (see also Table S3A–D in File S1 ). B) Ontology analysis and pathway analysis of RXRα peaks with score > 6 or

    Article Snippet: ChIP-quality anti-RXRα antibody (D-20,sc-774X, Santa Cruz Biotechnology, Santa Cruz, CA) and RNA Pol2 (ab24758, Abcam) were used.

    Techniques: Binding Assay

    Sexual dimorphic RXRα-dependent gene regulation in mouse liver. A) Scatter plot of RXRα scores correlating with Pol2 scores. Genes identified having a RXRα and Pol2 score > 6 for male and -

    Journal: PLoS ONE

    Article Title: Sexually Dimorphic Genome-Wide Binding of Retinoid X Receptor alpha (RXR?) Determines Male-Female Differences in the Expression of Hepatic Lipid Processing Genes in Mice

    doi: 10.1371/journal.pone.0071538

    Figure Lengend Snippet: Sexual dimorphic RXRα-dependent gene regulation in mouse liver. A) Scatter plot of RXRα scores correlating with Pol2 scores. Genes identified having a RXRα and Pol2 score > 6 for male and -

    Article Snippet: ChIP-quality anti-RXRα antibody (D-20,sc-774X, Santa Cruz Biotechnology, Santa Cruz, CA) and RNA Pol2 (ab24758, Abcam) were used.

    Techniques:

    Representatives of gender differential RXRαregulated genes. A) Predominant RXRα and Pol2 binding for Cyp7b1 in male mouse liver. Left-hand panel: Top 2 tracks show Pol2 binding along the Cyp7b1 gene-span in male and female mouse liver. Bottom 2 tracks show RXRα peaks binding upstream of the TSS of Cyp7b1 TSS. Male enriched RXRα peaks are indicated by arrows P1, P2 and P3. Right-hand panel: Sexual dimorphic RNA levels of Cyp7b1 were confirmed by qPCR (n = 5–6) (see also Fig. S2 and S3A–G ). B) Predominant RXRα and Pol2 binding for Pnpla3 in female mouse liver. Left-hand panel: Top 2 tracks show Pol2 binding along the Pnpla3 gene-span in male and female mouse liver. Bottom 2 tracks show RXRα peaks binding upstream of the TSS of Pnpla3 TSS. Female enriched RXRα peaks are indicated by arrows P1, P2, P3 and P4. Right-hand panel: Sexual dimorphic RNA levels of Pnpla3 were confirmed by qPCR (n = 5–6) (see also Fig. S2 , S3A–G , S7 and S8). *p

    Journal: PLoS ONE

    Article Title: Sexually Dimorphic Genome-Wide Binding of Retinoid X Receptor alpha (RXR?) Determines Male-Female Differences in the Expression of Hepatic Lipid Processing Genes in Mice

    doi: 10.1371/journal.pone.0071538

    Figure Lengend Snippet: Representatives of gender differential RXRαregulated genes. A) Predominant RXRα and Pol2 binding for Cyp7b1 in male mouse liver. Left-hand panel: Top 2 tracks show Pol2 binding along the Cyp7b1 gene-span in male and female mouse liver. Bottom 2 tracks show RXRα peaks binding upstream of the TSS of Cyp7b1 TSS. Male enriched RXRα peaks are indicated by arrows P1, P2 and P3. Right-hand panel: Sexual dimorphic RNA levels of Cyp7b1 were confirmed by qPCR (n = 5–6) (see also Fig. S2 and S3A–G ). B) Predominant RXRα and Pol2 binding for Pnpla3 in female mouse liver. Left-hand panel: Top 2 tracks show Pol2 binding along the Pnpla3 gene-span in male and female mouse liver. Bottom 2 tracks show RXRα peaks binding upstream of the TSS of Pnpla3 TSS. Female enriched RXRα peaks are indicated by arrows P1, P2, P3 and P4. Right-hand panel: Sexual dimorphic RNA levels of Pnpla3 were confirmed by qPCR (n = 5–6) (see also Fig. S2 , S3A–G , S7 and S8). *p

    Article Snippet: ChIP-quality anti-RXRα antibody (D-20,sc-774X, Santa Cruz Biotechnology, Santa Cruz, CA) and RNA Pol2 (ab24758, Abcam) were used.

    Techniques: Binding Assay, Real-time Polymerase Chain Reaction

    RXRα is required for R -etodolac-induced apoptosis. ( a ) Inhibition of RXRα expression by RXRα siRNA. LNCaP cells were untransfected or transfected with a pool of RXRα siRNAs or control GFP siRNA for 48 h. Cell lysates were

    Journal:

    Article Title: The R-enantiomer of the nonsteroidal antiinflammatory drug etodolac binds retinoid X receptor and induces tumor-selective apoptosis

    doi: 10.1073/pnas.0409721102

    Figure Lengend Snippet: RXRα is required for R -etodolac-induced apoptosis. ( a ) Inhibition of RXRα expression by RXRα siRNA. LNCaP cells were untransfected or transfected with a pool of RXRα siRNAs or control GFP siRNA for 48 h. Cell lysates were

    Article Snippet: The prostates were removed, and serial frozen sections were assayed for terminal deoxyribonucleotidyl transferase (TdT; TUNEL; Chemicon) or stained with anti-human RXRα (D20; Santa Cruz Biotechnology), followed by staining with DAPI (50 μg/ml; Sigma) containing DNase-free RNase A (100 μg/ml; Boehringer Mannheim) to visualize the nuclei and examined by fluorescence microscopy ( , , ).

    Techniques: Inhibition, Expressing, Transfection

    R -etodolac binds to RXRα. ( a ) R -etodolac competes with 9- cis -RA for binding to RXRα. Human RXRα LBD was incubated with 1 nM [ 3 H]9- cis -RA in the presence of different concentrations of unlabeled 9- cis -RA (•) or R -etodolac

    Journal:

    Article Title: The R-enantiomer of the nonsteroidal antiinflammatory drug etodolac binds retinoid X receptor and induces tumor-selective apoptosis

    doi: 10.1073/pnas.0409721102

    Figure Lengend Snippet: R -etodolac binds to RXRα. ( a ) R -etodolac competes with 9- cis -RA for binding to RXRα. Human RXRα LBD was incubated with 1 nM [ 3 H]9- cis -RA in the presence of different concentrations of unlabeled 9- cis -RA (•) or R -etodolac

    Article Snippet: The prostates were removed, and serial frozen sections were assayed for terminal deoxyribonucleotidyl transferase (TdT; TUNEL; Chemicon) or stained with anti-human RXRα (D20; Santa Cruz Biotechnology), followed by staining with DAPI (50 μg/ml; Sigma) containing DNase-free RNase A (100 μg/ml; Boehringer Mannheim) to visualize the nuclei and examined by fluorescence microscopy ( , , ).

    Techniques: Binding Assay, Incubation

    R -etodolac inhibits 9- cis -RA-induced transcriptional activity of RXRα. ( a ) Inhibition of RXRα homodimer activity by R -etodolac. CV-1 cells were cotransfected with or without an expression vector for RXRα (25 ng), a CAT reporter

    Journal:

    Article Title: The R-enantiomer of the nonsteroidal antiinflammatory drug etodolac binds retinoid X receptor and induces tumor-selective apoptosis

    doi: 10.1073/pnas.0409721102

    Figure Lengend Snippet: R -etodolac inhibits 9- cis -RA-induced transcriptional activity of RXRα. ( a ) Inhibition of RXRα homodimer activity by R -etodolac. CV-1 cells were cotransfected with or without an expression vector for RXRα (25 ng), a CAT reporter

    Article Snippet: The prostates were removed, and serial frozen sections were assayed for terminal deoxyribonucleotidyl transferase (TdT; TUNEL; Chemicon) or stained with anti-human RXRα (D20; Santa Cruz Biotechnology), followed by staining with DAPI (50 μg/ml; Sigma) containing DNase-free RNase A (100 μg/ml; Boehringer Mannheim) to visualize the nuclei and examined by fluorescence microscopy ( , , ).

    Techniques: Activity Assay, Inhibition, Expressing, Plasmid Preparation

    R -etodolac induces RXRα degradation. ( a ) Diminished RXRα staining in prostate of R -etodolac-fed TRAMP mice. For 2 weeks, we fed 6- to 7-month-old TRAMP mice R -etodolac-supplemented chow or control chow. The prostate tissues were immunostained

    Journal:

    Article Title: The R-enantiomer of the nonsteroidal antiinflammatory drug etodolac binds retinoid X receptor and induces tumor-selective apoptosis

    doi: 10.1073/pnas.0409721102

    Figure Lengend Snippet: R -etodolac induces RXRα degradation. ( a ) Diminished RXRα staining in prostate of R -etodolac-fed TRAMP mice. For 2 weeks, we fed 6- to 7-month-old TRAMP mice R -etodolac-supplemented chow or control chow. The prostate tissues were immunostained

    Article Snippet: The prostates were removed, and serial frozen sections were assayed for terminal deoxyribonucleotidyl transferase (TdT; TUNEL; Chemicon) or stained with anti-human RXRα (D20; Santa Cruz Biotechnology), followed by staining with DAPI (50 μg/ml; Sigma) containing DNase-free RNase A (100 μg/ml; Boehringer Mannheim) to visualize the nuclei and examined by fluorescence microscopy ( , , ).

    Techniques: Staining, Mouse Assay

    The heterodimerization of hPXR S350D and RXRα is impaired

    Journal: Biochemical pharmacology

    Article Title: Serine 350 of Human Pregnane X Receptor Is Crucial for Its Heterodimerization with Retinoid X Receptor Alpha and Transactivation of Target Genes in Vitro and in Vivo

    doi: 10.1016/j.bcp.2015.06.018

    Figure Lengend Snippet: The heterodimerization of hPXR S350D and RXRα is impaired

    Article Snippet: For Western blotting, the membrane was blocked for 1 h with Odyssey blocking buffer (LI-COR Biosciences, Lincoln, NE), treated with mouse monoclonal antibodies to rat CYP3A11 (MAB10041, Millipore, Temecula, CA) [ ]) and FALG M2 (Sigma) and a rabbit anti-RXRα antibody (D20, Santa Cruz Biotechnology) [ ], and incubated with secondary goat anti-mouse antibody labeled with infrared dye (LI-COR Biosciences).

    Techniques: