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  • 94
    Novus Biologicals anti mouse rip3 antibody
    (A) Level of <t>RIP3</t> protein expression in human cancer cells (HeLa and HT-29) and murine fibroblast cells (NIH-3T3 and L929). Immunoblots against α-tubulin as a loading control. Relative band intensities are quantified and shown as average of fold changes versus that of HeLa or NIH-3T3 cells, respectively (n = 2). (B-D) Intracellular ROS level was determined by indicated oxidation-sensitive probes. For induction of necroptosis, HT-29 and L929 cells were treated with TNF-α (10 ng/ml)/BV6 (1 μM)/zVAD (20 μM), abbreviated as T/B/Z, and TNF-α (10 ng/ml)/zVAD (20 μM) combination, respectively. For apoptosis, HeLa cells were treated with TNF-α (10 ng/ml) plus cycloheximide (10 μg/ml) combination. Data in the graph are means ± SD of fluorescence intensities of 100-150 cells from three independent experiments. Mitochondrial superoxide anion and H 2 O 2 levels were measured using MitoSOX (B) and MitoPY1 (C), respectively. Level of cytosolic superoxide anion was measured using DHE (D). (E) Cytosolic H 2 O 2 level was measured in the HyPer-expressing cells. Fluorescence images were taken from the HyPer-expressing HT-29 and L929 cells. Data in the graph are means ± SD of fold change of ratio of fluorescence intensities at 488 nm and 405 nm of 100-120 cells from three independent experiments.
    Anti Mouse Rip3 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse rip3 antibody/product/Novus Biologicals
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mouse rip3 antibody - by Bioz Stars, 2024-07
    94/100 stars
      Buy from Supplier

    90
    ProSci Incorporated ripk3 antibody
    (A) Level of <t>RIP3</t> protein expression in human cancer cells (HeLa and HT-29) and murine fibroblast cells (NIH-3T3 and L929). Immunoblots against α-tubulin as a loading control. Relative band intensities are quantified and shown as average of fold changes versus that of HeLa or NIH-3T3 cells, respectively (n = 2). (B-D) Intracellular ROS level was determined by indicated oxidation-sensitive probes. For induction of necroptosis, HT-29 and L929 cells were treated with TNF-α (10 ng/ml)/BV6 (1 μM)/zVAD (20 μM), abbreviated as T/B/Z, and TNF-α (10 ng/ml)/zVAD (20 μM) combination, respectively. For apoptosis, HeLa cells were treated with TNF-α (10 ng/ml) plus cycloheximide (10 μg/ml) combination. Data in the graph are means ± SD of fluorescence intensities of 100-150 cells from three independent experiments. Mitochondrial superoxide anion and H 2 O 2 levels were measured using MitoSOX (B) and MitoPY1 (C), respectively. Level of cytosolic superoxide anion was measured using DHE (D). (E) Cytosolic H 2 O 2 level was measured in the HyPer-expressing cells. Fluorescence images were taken from the HyPer-expressing HT-29 and L929 cells. Data in the graph are means ± SD of fold change of ratio of fluorescence intensities at 488 nm and 405 nm of 100-120 cells from three independent experiments.
    Ripk3 Antibody, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ripk3 antibody/product/ProSci Incorporated
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ripk3 antibody - by Bioz Stars, 2024-07
    90/100 stars
      Buy from Supplier

    90
    ProSci Incorporated rip3 antibody
    (A) Level of <t>RIP3</t> protein expression in human cancer cells (HeLa and HT-29) and murine fibroblast cells (NIH-3T3 and L929). Immunoblots against α-tubulin as a loading control. Relative band intensities are quantified and shown as average of fold changes versus that of HeLa or NIH-3T3 cells, respectively (n = 2). (B-D) Intracellular ROS level was determined by indicated oxidation-sensitive probes. For induction of necroptosis, HT-29 and L929 cells were treated with TNF-α (10 ng/ml)/BV6 (1 μM)/zVAD (20 μM), abbreviated as T/B/Z, and TNF-α (10 ng/ml)/zVAD (20 μM) combination, respectively. For apoptosis, HeLa cells were treated with TNF-α (10 ng/ml) plus cycloheximide (10 μg/ml) combination. Data in the graph are means ± SD of fluorescence intensities of 100-150 cells from three independent experiments. Mitochondrial superoxide anion and H 2 O 2 levels were measured using MitoSOX (B) and MitoPY1 (C), respectively. Level of cytosolic superoxide anion was measured using DHE (D). (E) Cytosolic H 2 O 2 level was measured in the HyPer-expressing cells. Fluorescence images were taken from the HyPer-expressing HT-29 and L929 cells. Data in the graph are means ± SD of fold change of ratio of fluorescence intensities at 488 nm and 405 nm of 100-120 cells from three independent experiments.
    Rip3 Antibody, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rip3 antibody/product/ProSci Incorporated
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rip3 antibody - by Bioz Stars, 2024-07
    90/100 stars
      Buy from Supplier

    93
    Proteintech antiripk3
    (A) Level of <t>RIP3</t> protein expression in human cancer cells (HeLa and HT-29) and murine fibroblast cells (NIH-3T3 and L929). Immunoblots against α-tubulin as a loading control. Relative band intensities are quantified and shown as average of fold changes versus that of HeLa or NIH-3T3 cells, respectively (n = 2). (B-D) Intracellular ROS level was determined by indicated oxidation-sensitive probes. For induction of necroptosis, HT-29 and L929 cells were treated with TNF-α (10 ng/ml)/BV6 (1 μM)/zVAD (20 μM), abbreviated as T/B/Z, and TNF-α (10 ng/ml)/zVAD (20 μM) combination, respectively. For apoptosis, HeLa cells were treated with TNF-α (10 ng/ml) plus cycloheximide (10 μg/ml) combination. Data in the graph are means ± SD of fluorescence intensities of 100-150 cells from three independent experiments. Mitochondrial superoxide anion and H 2 O 2 levels were measured using MitoSOX (B) and MitoPY1 (C), respectively. Level of cytosolic superoxide anion was measured using DHE (D). (E) Cytosolic H 2 O 2 level was measured in the HyPer-expressing cells. Fluorescence images were taken from the HyPer-expressing HT-29 and L929 cells. Data in the graph are means ± SD of fold change of ratio of fluorescence intensities at 488 nm and 405 nm of 100-120 cells from three independent experiments.
    Antiripk3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antiripk3/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antiripk3 - by Bioz Stars, 2024-07
    93/100 stars
      Buy from Supplier

    93
    Proteintech anti caspase8
    PCR primers for quantitative real-time PCR.
    Anti Caspase8, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti caspase8/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti caspase8 - by Bioz Stars, 2024-07
    93/100 stars
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    Image Search Results


    (A) Level of RIP3 protein expression in human cancer cells (HeLa and HT-29) and murine fibroblast cells (NIH-3T3 and L929). Immunoblots against α-tubulin as a loading control. Relative band intensities are quantified and shown as average of fold changes versus that of HeLa or NIH-3T3 cells, respectively (n = 2). (B-D) Intracellular ROS level was determined by indicated oxidation-sensitive probes. For induction of necroptosis, HT-29 and L929 cells were treated with TNF-α (10 ng/ml)/BV6 (1 μM)/zVAD (20 μM), abbreviated as T/B/Z, and TNF-α (10 ng/ml)/zVAD (20 μM) combination, respectively. For apoptosis, HeLa cells were treated with TNF-α (10 ng/ml) plus cycloheximide (10 μg/ml) combination. Data in the graph are means ± SD of fluorescence intensities of 100-150 cells from three independent experiments. Mitochondrial superoxide anion and H 2 O 2 levels were measured using MitoSOX (B) and MitoPY1 (C), respectively. Level of cytosolic superoxide anion was measured using DHE (D). (E) Cytosolic H 2 O 2 level was measured in the HyPer-expressing cells. Fluorescence images were taken from the HyPer-expressing HT-29 and L929 cells. Data in the graph are means ± SD of fold change of ratio of fluorescence intensities at 488 nm and 405 nm of 100-120 cells from three independent experiments.

    Journal: Molecules and Cells

    Article Title: RIP3-Dependent Accumulation of Mitochondrial Superoxide Anions in TNF-α-Induced Necroptosis

    doi: 10.14348/molcells.2021.0260

    Figure Lengend Snippet: (A) Level of RIP3 protein expression in human cancer cells (HeLa and HT-29) and murine fibroblast cells (NIH-3T3 and L929). Immunoblots against α-tubulin as a loading control. Relative band intensities are quantified and shown as average of fold changes versus that of HeLa or NIH-3T3 cells, respectively (n = 2). (B-D) Intracellular ROS level was determined by indicated oxidation-sensitive probes. For induction of necroptosis, HT-29 and L929 cells were treated with TNF-α (10 ng/ml)/BV6 (1 μM)/zVAD (20 μM), abbreviated as T/B/Z, and TNF-α (10 ng/ml)/zVAD (20 μM) combination, respectively. For apoptosis, HeLa cells were treated with TNF-α (10 ng/ml) plus cycloheximide (10 μg/ml) combination. Data in the graph are means ± SD of fluorescence intensities of 100-150 cells from three independent experiments. Mitochondrial superoxide anion and H 2 O 2 levels were measured using MitoSOX (B) and MitoPY1 (C), respectively. Level of cytosolic superoxide anion was measured using DHE (D). (E) Cytosolic H 2 O 2 level was measured in the HyPer-expressing cells. Fluorescence images were taken from the HyPer-expressing HT-29 and L929 cells. Data in the graph are means ± SD of fold change of ratio of fluorescence intensities at 488 nm and 405 nm of 100-120 cells from three independent experiments.

    Article Snippet: Anti-mouse RIP3 antibody (Cat. No. NBP1-77299) was purchased from NOVUS Biologicals (USA).

    Techniques: Expressing, Western Blot, Fluorescence

    (A) Knockdown of RIP3 expression in HT-29 cells by siRNAs specific to human RIP3. Level of RIP3 was shown by immunoblotting. Control siRNA (CON) is against firefly luciferase. Data in the graph are means of the percentage of ralative intensity of RIP3 band after being normailized by corresponding α-tubulin bands (n = 3). (B) The siRNA-transfected HT29 cells were treated with DMSO or TNF-α/BV6/zVAD (T/B/Z) combination for 6 h. The cells were labeled with propidium iodide (PI) and annexin-V followed by fluorescence-activated cell sorting (FACS) analysis. Data in the graph are means ± SD of the percentage of necroptotic cells (n = 3, * P < 0.005). (C and D) The siRNA-transfected HT-29 cells were treated with vehicle control (DMSO) or T/B/Z for 2 h and stained with MitoSOX. The flurescence (C) was analyzed by a confocal microscopy. Data in the graph are means ± SD of relative fluorescence intensities of 45 cells from three independent experiments (* P < 0.001). Scale bar = 20 μm.

    Journal: Molecules and Cells

    Article Title: RIP3-Dependent Accumulation of Mitochondrial Superoxide Anions in TNF-α-Induced Necroptosis

    doi: 10.14348/molcells.2021.0260

    Figure Lengend Snippet: (A) Knockdown of RIP3 expression in HT-29 cells by siRNAs specific to human RIP3. Level of RIP3 was shown by immunoblotting. Control siRNA (CON) is against firefly luciferase. Data in the graph are means of the percentage of ralative intensity of RIP3 band after being normailized by corresponding α-tubulin bands (n = 3). (B) The siRNA-transfected HT29 cells were treated with DMSO or TNF-α/BV6/zVAD (T/B/Z) combination for 6 h. The cells were labeled with propidium iodide (PI) and annexin-V followed by fluorescence-activated cell sorting (FACS) analysis. Data in the graph are means ± SD of the percentage of necroptotic cells (n = 3, * P < 0.005). (C and D) The siRNA-transfected HT-29 cells were treated with vehicle control (DMSO) or T/B/Z for 2 h and stained with MitoSOX. The flurescence (C) was analyzed by a confocal microscopy. Data in the graph are means ± SD of relative fluorescence intensities of 45 cells from three independent experiments (* P < 0.001). Scale bar = 20 μm.

    Article Snippet: Anti-mouse RIP3 antibody (Cat. No. NBP1-77299) was purchased from NOVUS Biologicals (USA).

    Techniques: Expressing, Western Blot, Luciferase, Transfection, Labeling, Fluorescence, FACS, Staining, Confocal Microscopy

    PCR primers for quantitative real-time PCR.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: New insights of necroptosis and immune infiltration in sepsis-induced myocardial dysfunction from bioinformatics analysis through RNA-seq in mice

    doi: 10.3389/fcimb.2022.1068324

    Figure Lengend Snippet: PCR primers for quantitative real-time PCR.

    Article Snippet: The membranes were blocked with 5% skimmed milk for 1 h. Then, the membranes were incubated overnight at 4°C with the following primary antibodies: anti-MLKL antibody (1:2,000, Abcam, ab243142), anti-RIPK3 (1:1000, Proteintech, 17563-1-AP), anti-TNFα antibody (1:1,000, Proteintech, 17590-1-AP), anti-Caspase8 (1:2,000, Proteintech, Abcam, ab108333), anti-IL-1β antibody (1:500, affbiotech, AF5103), and anti-TLR3 (1:500, Abcam, ab62566).

    Techniques:

    Necroptosis is activated in the heart tissues of the sepsis-induced myocardial dysfunction model mice. (A) QRT-PCR analysis results show the relative expression levels of TNF, IL-1β, MLKL, RIPK3, CASPASE8, TLR3, TRADD, STAT1, NLRP3 and FAS mRNAs in the heart tissues from the control and SIMD group mice (n = 3 per group). GAPDH was used as the internal control and used for normalizing the mRNA expression data. (B) Western blot analysis results show the expression levels of TNFα, IL-1β, MLKL, RIPK3, CASPASE8, and TLR3 proteins in the heart tissues from the control and SIMD group mice (n = 3 per group). GAPDH was used as the loading control. (C) Representative images show the immunohistochemical analysis of CASPASE1, GSDMD, MLKL, and RIPK3 protein levels in the heart tissues from the control and SIMD group mice. Scale bar: 50μm. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: New insights of necroptosis and immune infiltration in sepsis-induced myocardial dysfunction from bioinformatics analysis through RNA-seq in mice

    doi: 10.3389/fcimb.2022.1068324

    Figure Lengend Snippet: Necroptosis is activated in the heart tissues of the sepsis-induced myocardial dysfunction model mice. (A) QRT-PCR analysis results show the relative expression levels of TNF, IL-1β, MLKL, RIPK3, CASPASE8, TLR3, TRADD, STAT1, NLRP3 and FAS mRNAs in the heart tissues from the control and SIMD group mice (n = 3 per group). GAPDH was used as the internal control and used for normalizing the mRNA expression data. (B) Western blot analysis results show the expression levels of TNFα, IL-1β, MLKL, RIPK3, CASPASE8, and TLR3 proteins in the heart tissues from the control and SIMD group mice (n = 3 per group). GAPDH was used as the loading control. (C) Representative images show the immunohistochemical analysis of CASPASE1, GSDMD, MLKL, and RIPK3 protein levels in the heart tissues from the control and SIMD group mice. Scale bar: 50μm. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Article Snippet: The membranes were blocked with 5% skimmed milk for 1 h. Then, the membranes were incubated overnight at 4°C with the following primary antibodies: anti-MLKL antibody (1:2,000, Abcam, ab243142), anti-RIPK3 (1:1000, Proteintech, 17563-1-AP), anti-TNFα antibody (1:1,000, Proteintech, 17590-1-AP), anti-Caspase8 (1:2,000, Proteintech, Abcam, ab108333), anti-IL-1β antibody (1:500, affbiotech, AF5103), and anti-TLR3 (1:500, Abcam, ab62566).

    Techniques: Quantitative RT-PCR, Expressing, Western Blot, Immunohistochemical staining