anti phospho p70s6k Search Results


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  • 96
    Cell Signaling Technology Inc rabbit anti phospho s6k thr398
    (A) Influence of P . berghei infection on the TOR pathway by Western blot analysis. Phosphorylation levels of <t>S6K</t> from fat bodies 12 h and 24 h post-normal (NB) and infectious blood meal (IB) were analyzed using anti-Phospho-S6K antibody. An . stephensi S6K and ACTIN were used as internal controls (left panel). The intensity of phosphorylated S6K was normalized to that of S6K. The relative intensity of phosphorylated S6K in IB-fed mosquitoes was normalized to that of NB-fed mosquitoes (right panel). Results were pooled from three independent experiments. (B) Schematic overview of rapamycin microinjection of An . stephensi . Vehicle solution was injected as a control. (C) The influence of rapamycin treatment on the mosquito TOR pathway. Phosphorylation levels of S6K from fat bodies 24 h post-blood meal were analyzed. Quantification of <t>p-S6K</t> signal intensity in rapamycin-treated mosquitoes was normalized to that of controls. The results are from two independent experiments. (D) Influence of rapamycin treatment on Plasmodium infection. Data were pooled from three independent replicates. (E) Influence of TOR knockdown on the activity of the TOR signaling pathway. Relative quantification of p-S6K signal intensity was from two independent replicates. (F) Influence of TOR knockdown on Plasmodium infection. The data were pooled from three independent biological experiments. Horizontal black bars indicate median oocyst numbers. Each dot represents an individual mosquito. Error bars indicate standard errors. Significance was determined by Student’s t- test in (A), (C), and (E) and by Mann-Whitney tests in (D) and (F); *P<0.05, **P<0.01, ****P<0.0001.
    Rabbit Anti Phospho S6k Thr398, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Aviva Systems p p70 s6k 9234
    Summary of statistical analyses.
    P P70 S6k 9234, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p p70 s6k 9234/product/Aviva Systems
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    92
    Aviva Systems oaaf07416
    Key resources table
    Oaaf07416, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oaaf07416/product/Aviva Systems
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    90
    Biorbyt phospho s6k1 t389
    Key resources table
    Phospho S6k1 T389, supplied by Biorbyt, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Upstate Biotechnology Inc phospho p70s6k
    Training-related increase in <t>phospho-p70s6K</t> is specific to the learned association. Bars represent mean optical density (OD) values (±SEM) expressed as a percentage of control. a, b, There were no changes in <t>total-p70s6K</t> expression after immediate or delayed shock (a), but a significant increase in phospho-p70s6K was seen only in rats that received a delayed shock (b). Immunofluorescence images (20× magnification) from single subjects killed from their home cage (HC) (c) or given a single shock immediately (IMM) or 2 min after placement into the chamber (DLY) are shown. d, e, Although controls (d) received the same shock and stimulus exposure, phosphorylation of the enzyme in the lateral amygdala was seen only in rats that formed new long-term memory for fear conditioning (e) (*p < 0.05).
    Phospho P70s6k, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) Influence of P . berghei infection on the TOR pathway by Western blot analysis. Phosphorylation levels of S6K from fat bodies 12 h and 24 h post-normal (NB) and infectious blood meal (IB) were analyzed using anti-Phospho-S6K antibody. An . stephensi S6K and ACTIN were used as internal controls (left panel). The intensity of phosphorylated S6K was normalized to that of S6K. The relative intensity of phosphorylated S6K in IB-fed mosquitoes was normalized to that of NB-fed mosquitoes (right panel). Results were pooled from three independent experiments. (B) Schematic overview of rapamycin microinjection of An . stephensi . Vehicle solution was injected as a control. (C) The influence of rapamycin treatment on the mosquito TOR pathway. Phosphorylation levels of S6K from fat bodies 24 h post-blood meal were analyzed. Quantification of p-S6K signal intensity in rapamycin-treated mosquitoes was normalized to that of controls. The results are from two independent experiments. (D) Influence of rapamycin treatment on Plasmodium infection. Data were pooled from three independent replicates. (E) Influence of TOR knockdown on the activity of the TOR signaling pathway. Relative quantification of p-S6K signal intensity was from two independent replicates. (F) Influence of TOR knockdown on Plasmodium infection. The data were pooled from three independent biological experiments. Horizontal black bars indicate median oocyst numbers. Each dot represents an individual mosquito. Error bars indicate standard errors. Significance was determined by Student’s t- test in (A), (C), and (E) and by Mann-Whitney tests in (D) and (F); *P<0.05, **P<0.01, ****P<0.0001.

    Journal: PLoS Pathogens

    Article Title: Rapamycin inhibits pathogen transmission in mosquitoes by promoting immune activation

    doi: 10.1371/journal.ppat.1009353

    Figure Lengend Snippet: (A) Influence of P . berghei infection on the TOR pathway by Western blot analysis. Phosphorylation levels of S6K from fat bodies 12 h and 24 h post-normal (NB) and infectious blood meal (IB) were analyzed using anti-Phospho-S6K antibody. An . stephensi S6K and ACTIN were used as internal controls (left panel). The intensity of phosphorylated S6K was normalized to that of S6K. The relative intensity of phosphorylated S6K in IB-fed mosquitoes was normalized to that of NB-fed mosquitoes (right panel). Results were pooled from three independent experiments. (B) Schematic overview of rapamycin microinjection of An . stephensi . Vehicle solution was injected as a control. (C) The influence of rapamycin treatment on the mosquito TOR pathway. Phosphorylation levels of S6K from fat bodies 24 h post-blood meal were analyzed. Quantification of p-S6K signal intensity in rapamycin-treated mosquitoes was normalized to that of controls. The results are from two independent experiments. (D) Influence of rapamycin treatment on Plasmodium infection. Data were pooled from three independent replicates. (E) Influence of TOR knockdown on the activity of the TOR signaling pathway. Relative quantification of p-S6K signal intensity was from two independent replicates. (F) Influence of TOR knockdown on Plasmodium infection. The data were pooled from three independent biological experiments. Horizontal black bars indicate median oocyst numbers. Each dot represents an individual mosquito. Error bars indicate standard errors. Significance was determined by Student’s t- test in (A), (C), and (E) and by Mann-Whitney tests in (D) and (F); *P<0.05, **P<0.01, ****P<0.0001.

    Article Snippet: Antibodies used for TOR signaling were rabbit anti-phospho-S6K (Thr398) (1:1000) (Cell Signaling), rabbit anti-S6K (1:1000), and rabbit anti-actin (1:2000) (Sungenebiotech, China).

    Techniques: Infection, Western Blot, Injection, Activity Assay, MANN-WHITNEY

    (A) Schematic overview of exposing mosquitoes to a rapamycin-treated surface. A solvent-coated surface was used as a control. t E , exposure time. (B) Oocyst numbers in mosquitoes exposed to rapamycin (0.77 mmol/m 2 ) (red dots) or solvent (black dots) coated surfaces for 60 min. (C) Mosquitoes were exposed to a 0.77 mmol/m 2 rapamycin-coated Petri dish for 30 min, 10 min, and 6 min. Data were pooled from two independent replicates (B, C). (D - H) Mosquitoes were incubated with a 0.77 mmol/m 2 rapamycin- or solvent-coated surface for 10 min. (D) Survival curve of mosquitoes exposed to rapamycin (n = 38) or solvent (n = 36) coated surfaces. (E) Western blot analysis of S6K phosphorylation in fat bodies collected from rapamycin-exposed mosquitoes and controls. The bar chart represents relative quantification of signal intensity of p-S6K from two independent replicates as determined by ImageJ software. Error bars indicate standard errors. (F) Sporozoite numbers of rapamycin-treated mosquitoes (red dots) and controls (black dots). Data were pooled from two independent replicates. (G) Ovaries were dissected at 48 h post-normal blood meal from rapamycin-exposed and control mosquitoes. Scale bar, 200 μm. (H) Mean number of eggs laid by 25–35 gravid rapamycin-exposed and control mosquitoes. Data were pooled from three independent replicates. Results from one of three independent experiments are shown (D, G). Horizontal black bars indicate the median values. Significance was determined by Mann-Whitney tests in (B), (C), and (F), by a Log-rank (Mantel-Cox) test in (D), and by Student’s t -test in (E) and (H); *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.

    Journal: PLoS Pathogens

    Article Title: Rapamycin inhibits pathogen transmission in mosquitoes by promoting immune activation

    doi: 10.1371/journal.ppat.1009353

    Figure Lengend Snippet: (A) Schematic overview of exposing mosquitoes to a rapamycin-treated surface. A solvent-coated surface was used as a control. t E , exposure time. (B) Oocyst numbers in mosquitoes exposed to rapamycin (0.77 mmol/m 2 ) (red dots) or solvent (black dots) coated surfaces for 60 min. (C) Mosquitoes were exposed to a 0.77 mmol/m 2 rapamycin-coated Petri dish for 30 min, 10 min, and 6 min. Data were pooled from two independent replicates (B, C). (D - H) Mosquitoes were incubated with a 0.77 mmol/m 2 rapamycin- or solvent-coated surface for 10 min. (D) Survival curve of mosquitoes exposed to rapamycin (n = 38) or solvent (n = 36) coated surfaces. (E) Western blot analysis of S6K phosphorylation in fat bodies collected from rapamycin-exposed mosquitoes and controls. The bar chart represents relative quantification of signal intensity of p-S6K from two independent replicates as determined by ImageJ software. Error bars indicate standard errors. (F) Sporozoite numbers of rapamycin-treated mosquitoes (red dots) and controls (black dots). Data were pooled from two independent replicates. (G) Ovaries were dissected at 48 h post-normal blood meal from rapamycin-exposed and control mosquitoes. Scale bar, 200 μm. (H) Mean number of eggs laid by 25–35 gravid rapamycin-exposed and control mosquitoes. Data were pooled from three independent replicates. Results from one of three independent experiments are shown (D, G). Horizontal black bars indicate the median values. Significance was determined by Mann-Whitney tests in (B), (C), and (F), by a Log-rank (Mantel-Cox) test in (D), and by Student’s t -test in (E) and (H); *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.

    Article Snippet: Antibodies used for TOR signaling were rabbit anti-phospho-S6K (Thr398) (1:1000) (Cell Signaling), rabbit anti-S6K (1:1000), and rabbit anti-actin (1:2000) (Sungenebiotech, China).

    Techniques: Incubation, Western Blot, Software, MANN-WHITNEY

    Summary of statistical analyses.

    Journal: Journal of neurochemistry

    Article Title: Effects of the presence and absence of amino acids on translation, signaling and long-term depression in hippocampal slices from Fmr1 knockout mice

    doi: 10.1111/jnc.14874

    Figure Lengend Snippet: Summary of statistical analyses.

    Article Snippet: Primary antibodies (Cell Signaling, Danvers, MA) were: p-ERK1/2 (4370) (RRID:AB_2315112), ERK (4695) (RRID:AB_390779), p-eIF2α (3398) (RRID:AB_2096481), eIF2α (5324) (RRID:AB_10692650), p-mTOR (5536) (RRID: AB_10691552), mTOR (2983) (RRID: AB_2105622), p-p70 S6K (9234) (RRID: AB_2269803), p70 S6K (2708) (RRID: AB_390722), p-S6 235/236 (2211) (RRID: AB_331679), p-S6 240/244 (2215) (RRID: AB_331682), S6 (2217) (RRID: AB_331355), p-Akt (4060) (RRID: AB_2315049), Akt (9272) (RRID:AB_329827), p-GCN2 (OABF01173; Aviva Systems Biology, San Diego, CA, US) (RRID:AB_2801396) and GCN2 (ab137543; Abcam, Cambridge, UK) (RRID:AB_2801397).

    Techniques:

    Effect of treatment with DHPG (100 μM) on levels of p70S6k (A), p-p70S6k (B), and p-p70S6k/Total (C) in hippocampal slice preparations from WT and Fmr1 KO mice in amino acid replete and amino acid starvation conditions. Measurements were made in nine mice for each group. A. In the AAR condition one WT DHPG treated slice and one Fmr1 KO DHPG treated slice were excluded as outliers. In the AAS condition one WT Control slice was excluded as an outlier, and one Fmr1 KO DHPG treated slice was excluded because of an artifact on the Western blot. B. In the AAR condition, one Fmr1 KO Control slice and one Fmr1 KO DHPG treated slice were excluded as outliers. In the AAS condition one WT DHPG treated slice was excluded due to an artifact on the Western blot, and one Fmr1 KO Control slice was excluded as an outlier. C. All exclusions were a result of prior exclusions of either the p-p70S6k or total p70S6k. In the AAR condition, one WT DHPG treated slice, one Fmr1 KO Control slice and two Fmr1 KO DHPG treated slices were excluded. In the AAS condition one WT Control slice, one WT DHPG treated slice, one Fmr1 KO Control slice, and one Fmr1 KO DHPG treated slice were excluded. Data were analyzed by means of a three-way ANOVA with amino acid condition (AAR, AAS), genotype (WT, Fmr1 KO), and treatment (+/− DHPG) as between subject factors (Table 2). A. For p70S6k, none of the interactions or main effects were statistically significant. B. For p-p70S6k, the amino acid condition x genotype x treatment interaction was statistically significant (p = 0.024). We probed for individual group and condition differences by means of Bonferroni-corrected post hoc t-tests. Statistically significant effects are indicated on the figure: *, 0.01 ≤ p ≤ 0.05. C. For p-p70S6k/Total, the amino acid condition x genotype x treatment interaction was statistically significant (p = 0.050). We probed for individual group and condition differences by means of Bonferroni-corrected post hoc t-tests. Statistically significant effects are indicated on the figure: *, 0.01 ≤ p ≤ 0.05.

    Journal: Journal of neurochemistry

    Article Title: Effects of the presence and absence of amino acids on translation, signaling and long-term depression in hippocampal slices from Fmr1 knockout mice

    doi: 10.1111/jnc.14874

    Figure Lengend Snippet: Effect of treatment with DHPG (100 μM) on levels of p70S6k (A), p-p70S6k (B), and p-p70S6k/Total (C) in hippocampal slice preparations from WT and Fmr1 KO mice in amino acid replete and amino acid starvation conditions. Measurements were made in nine mice for each group. A. In the AAR condition one WT DHPG treated slice and one Fmr1 KO DHPG treated slice were excluded as outliers. In the AAS condition one WT Control slice was excluded as an outlier, and one Fmr1 KO DHPG treated slice was excluded because of an artifact on the Western blot. B. In the AAR condition, one Fmr1 KO Control slice and one Fmr1 KO DHPG treated slice were excluded as outliers. In the AAS condition one WT DHPG treated slice was excluded due to an artifact on the Western blot, and one Fmr1 KO Control slice was excluded as an outlier. C. All exclusions were a result of prior exclusions of either the p-p70S6k or total p70S6k. In the AAR condition, one WT DHPG treated slice, one Fmr1 KO Control slice and two Fmr1 KO DHPG treated slices were excluded. In the AAS condition one WT Control slice, one WT DHPG treated slice, one Fmr1 KO Control slice, and one Fmr1 KO DHPG treated slice were excluded. Data were analyzed by means of a three-way ANOVA with amino acid condition (AAR, AAS), genotype (WT, Fmr1 KO), and treatment (+/− DHPG) as between subject factors (Table 2). A. For p70S6k, none of the interactions or main effects were statistically significant. B. For p-p70S6k, the amino acid condition x genotype x treatment interaction was statistically significant (p = 0.024). We probed for individual group and condition differences by means of Bonferroni-corrected post hoc t-tests. Statistically significant effects are indicated on the figure: *, 0.01 ≤ p ≤ 0.05. C. For p-p70S6k/Total, the amino acid condition x genotype x treatment interaction was statistically significant (p = 0.050). We probed for individual group and condition differences by means of Bonferroni-corrected post hoc t-tests. Statistically significant effects are indicated on the figure: *, 0.01 ≤ p ≤ 0.05.

    Article Snippet: Primary antibodies (Cell Signaling, Danvers, MA) were: p-ERK1/2 (4370) (RRID:AB_2315112), ERK (4695) (RRID:AB_390779), p-eIF2α (3398) (RRID:AB_2096481), eIF2α (5324) (RRID:AB_10692650), p-mTOR (5536) (RRID: AB_10691552), mTOR (2983) (RRID: AB_2105622), p-p70 S6K (9234) (RRID: AB_2269803), p70 S6K (2708) (RRID: AB_390722), p-S6 235/236 (2211) (RRID: AB_331679), p-S6 240/244 (2215) (RRID: AB_331682), S6 (2217) (RRID: AB_331355), p-Akt (4060) (RRID: AB_2315049), Akt (9272) (RRID:AB_329827), p-GCN2 (OABF01173; Aviva Systems Biology, San Diego, CA, US) (RRID:AB_2801396) and GCN2 (ab137543; Abcam, Cambridge, UK) (RRID:AB_2801397).

    Techniques: Western Blot

    Key resources table

    Journal: iScience

    Article Title: Mitochondrial complex I inhibitors suppress tumor growth through concomitant acidification of the intra- and extracellular environment

    doi: 10.1016/j.isci.2021.103497

    Figure Lengend Snippet: Key resources table

    Article Snippet: The deparaffinized sections were boiled in 0.01 M buffered sodium citrate solution (pH 6.0) for 10 min and subjected to Masson's Trichrome staining (Sigma-Aldrich) overnight or immunohistochemical staining with the following antibodies for 30 min: anti-a-SMA (1:2000, ab7817, Abcam), anti-Vimentin (1:400, sc-6260, Santa Cruz Biotechnology), anti-GFP (1:500, #2955, Cell Signaling Technology), anti-Ki-67 (1:200, ab16667, Abcam), anti-Phospho-p70 S6 Kinase (Thr 389) (1:50, OAAF07416, Aviva Systems Biology, San Diego, CA), and horseradish peroxidase-linked secondary antibodies.

    Techniques: Recombinant, Synthesized, Staining, Isolation, Activity Assay, Lactate Assay, Software

    Training-related increase in phospho-p70s6K is specific to the learned association. Bars represent mean optical density (OD) values (±SEM) expressed as a percentage of control. a, b, There were no changes in total-p70s6K expression after immediate or delayed shock (a), but a significant increase in phospho-p70s6K was seen only in rats that received a delayed shock (b). Immunofluorescence images (20× magnification) from single subjects killed from their home cage (HC) (c) or given a single shock immediately (IMM) or 2 min after placement into the chamber (DLY) are shown. d, e, Although controls (d) received the same shock and stimulus exposure, phosphorylation of the enzyme in the lateral amygdala was seen only in rats that formed new long-term memory for fear conditioning (e) (*p < 0.05).

    Journal: The Journal of Neuroscience

    Article Title: Translational Control via the Mammalian Target of Rapamycin Pathway Is Critical for the Formation and Stability of Long-Term Fear Memory in Amygdala Neurons

    doi: 10.1523/JNEUROSCI.4209-06.2006

    Figure Lengend Snippet: Training-related increase in phospho-p70s6K is specific to the learned association. Bars represent mean optical density (OD) values (±SEM) expressed as a percentage of control. a, b, There were no changes in total-p70s6K expression after immediate or delayed shock (a), but a significant increase in phospho-p70s6K was seen only in rats that received a delayed shock (b). Immunofluorescence images (20× magnification) from single subjects killed from their home cage (HC) (c) or given a single shock immediately (IMM) or 2 min after placement into the chamber (DLY) are shown. d, e, Although controls (d) received the same shock and stimulus exposure, phosphorylation of the enzyme in the lateral amygdala was seen only in rats that formed new long-term memory for fear conditioning (e) (*p < 0.05).

    Article Snippet: Slices were incubated overnight in 500 μl of primary antibody diluted 1:50 against phospho-p70s6K (Thr 412) (Upstate Biotechnology).

    Techniques: Expressing, Immunofluorescence

    Time-dependent activation of phospho-p70s6K in the amygdala after fear conditioning. Bars represent mean optical density (OD) values (±SEM) expressed as a percentage of control. a, Rats killed at 30 and 60 min after fear conditioning showed significantly more phospho-p70s6K expression in the amygdala compared with home cage (HC) controls or animals killed 10 min after conditioning. This increase in phospho-p70s6K seen 60 min after training is reduced by an immediate posttraining injection of RAP (b). c, d, No differences between groups were observed when the same samples were probed with an antibody for total p70s6K (c), and no changes in total p70s6K expression were seen in the amygdala after RAP infusion (d) (*p < 0.05).

    Journal: The Journal of Neuroscience

    Article Title: Translational Control via the Mammalian Target of Rapamycin Pathway Is Critical for the Formation and Stability of Long-Term Fear Memory in Amygdala Neurons

    doi: 10.1523/JNEUROSCI.4209-06.2006

    Figure Lengend Snippet: Time-dependent activation of phospho-p70s6K in the amygdala after fear conditioning. Bars represent mean optical density (OD) values (±SEM) expressed as a percentage of control. a, Rats killed at 30 and 60 min after fear conditioning showed significantly more phospho-p70s6K expression in the amygdala compared with home cage (HC) controls or animals killed 10 min after conditioning. This increase in phospho-p70s6K seen 60 min after training is reduced by an immediate posttraining injection of RAP (b). c, d, No differences between groups were observed when the same samples were probed with an antibody for total p70s6K (c), and no changes in total p70s6K expression were seen in the amygdala after RAP infusion (d) (*p < 0.05).

    Article Snippet: Slices were incubated overnight in 500 μl of primary antibody diluted 1:50 against phospho-p70s6K (Thr 412) (Upstate Biotechnology).

    Techniques: Activation Assay, Expressing, Injection