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    • phospho mapk substrate  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc phospho mapk substrate
    Phospho Mapk Substrate, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho mapk substrate/product/Cell Signaling Technology Inc
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    anti phospho mapk cdk substrates  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc anti phospho mapk cdk substrates
    Identification of the phosphorylation site of Par3 by ERK2. A, The direct phosphorylation of Par3 by ERK2. Purified MBP or MBP-Par3 deletion mutants were incubated with recombinant ERK2 in the presence of [γ-32P]ATP in vitro. Samples were subjected to SDS-PAGE and silver staining (bottom) followed by autoradiography (top). Asterisks indicate intact MBP-fusion proteins. B, The phosphorylation of Par3 point mutants by ERK2. Purified MBP, MBP-Par3-4N-WT -S1116A, -T1145A, -S1316A, or -T1328A was incubated with recombinant ERK2 in the presence of [γ-32P]ATP in vitro. Samples were subjected to SDS-PAGE and silver staining (bottom) followed by autoradiography (top). Asterisks indicate intact MBP-fusion proteins. C, The alignment of ERK2 phosphorylation sites in Par3 homologs (rat, mouse, human). D, The phosphorylation of Par3-WT or -S1116A in COS7 cells. COS7 cells were transfected with EGFP-Par3-WT or -S1116A and cultured for 24 h. The cells were serum-starved for 24 h and then treated with or without 1 μm OA for 2 h. Cell lysates were incubated with anti-GFP antibody, and the immunoprecipitates were analyzed by immunoblotting with <t>anti-phospho-MAPK/CDK</t> substrates and anti-GFP antibodies.
    Anti Phospho Mapk Cdk Substrates, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho mapk cdk substrates/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti phospho mapk cdk substrates - by Bioz Stars, 2024-09
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    anti phospho mapk substrates motif  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc anti phospho mapk substrates motif
    Identification of the phosphorylation site of Par3 by ERK2. A, The direct phosphorylation of Par3 by ERK2. Purified MBP or MBP-Par3 deletion mutants were incubated with recombinant ERK2 in the presence of [γ-32P]ATP in vitro. Samples were subjected to SDS-PAGE and silver staining (bottom) followed by autoradiography (top). Asterisks indicate intact MBP-fusion proteins. B, The phosphorylation of Par3 point mutants by ERK2. Purified MBP, MBP-Par3-4N-WT -S1116A, -T1145A, -S1316A, or -T1328A was incubated with recombinant ERK2 in the presence of [γ-32P]ATP in vitro. Samples were subjected to SDS-PAGE and silver staining (bottom) followed by autoradiography (top). Asterisks indicate intact MBP-fusion proteins. C, The alignment of ERK2 phosphorylation sites in Par3 homologs (rat, mouse, human). D, The phosphorylation of Par3-WT or -S1116A in COS7 cells. COS7 cells were transfected with EGFP-Par3-WT or -S1116A and cultured for 24 h. The cells were serum-starved for 24 h and then treated with or without 1 μm OA for 2 h. Cell lysates were incubated with anti-GFP antibody, and the immunoprecipitates were analyzed by immunoblotting with <t>anti-phospho-MAPK/CDK</t> substrates and anti-GFP antibodies.
    Anti Phospho Mapk Substrates Motif, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho mapk substrates motif/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti phospho mapk substrates motif - by Bioz Stars, 2024-09
    86/100 stars
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    anti phospho mapk substrates cell signaling technology  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc anti phospho mapk substrates cell signaling technology
    Identification of the phosphorylation site of Par3 by ERK2. A, The direct phosphorylation of Par3 by ERK2. Purified MBP or MBP-Par3 deletion mutants were incubated with recombinant ERK2 in the presence of [γ-32P]ATP in vitro. Samples were subjected to SDS-PAGE and silver staining (bottom) followed by autoradiography (top). Asterisks indicate intact MBP-fusion proteins. B, The phosphorylation of Par3 point mutants by ERK2. Purified MBP, MBP-Par3-4N-WT -S1116A, -T1145A, -S1316A, or -T1328A was incubated with recombinant ERK2 in the presence of [γ-32P]ATP in vitro. Samples were subjected to SDS-PAGE and silver staining (bottom) followed by autoradiography (top). Asterisks indicate intact MBP-fusion proteins. C, The alignment of ERK2 phosphorylation sites in Par3 homologs (rat, mouse, human). D, The phosphorylation of Par3-WT or -S1116A in COS7 cells. COS7 cells were transfected with EGFP-Par3-WT or -S1116A and cultured for 24 h. The cells were serum-starved for 24 h and then treated with or without 1 μm OA for 2 h. Cell lysates were incubated with anti-GFP antibody, and the immunoprecipitates were analyzed by immunoblotting with <t>anti-phospho-MAPK/CDK</t> substrates and anti-GFP antibodies.
    Anti Phospho Mapk Substrates Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho mapk substrates cell signaling technology/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    sc 126 santa cruz biotechnology pdi adi spa 890 enzo life sciences phospho mapk cdk substrates  (Enzo Biochem)
    86
    Enzo Biochem sc 126 santa cruz biotechnology pdi adi spa 890 enzo life sciences phospho mapk cdk substrates
    Identification of the phosphorylation site of Par3 by ERK2. A, The direct phosphorylation of Par3 by ERK2. Purified MBP or MBP-Par3 deletion mutants were incubated with recombinant ERK2 in the presence of [γ-32P]ATP in vitro. Samples were subjected to SDS-PAGE and silver staining (bottom) followed by autoradiography (top). Asterisks indicate intact MBP-fusion proteins. B, The phosphorylation of Par3 point mutants by ERK2. Purified MBP, MBP-Par3-4N-WT -S1116A, -T1145A, -S1316A, or -T1328A was incubated with recombinant ERK2 in the presence of [γ-32P]ATP in vitro. Samples were subjected to SDS-PAGE and silver staining (bottom) followed by autoradiography (top). Asterisks indicate intact MBP-fusion proteins. C, The alignment of ERK2 phosphorylation sites in Par3 homologs (rat, mouse, human). D, The phosphorylation of Par3-WT or -S1116A in COS7 cells. COS7 cells were transfected with EGFP-Par3-WT or -S1116A and cultured for 24 h. The cells were serum-starved for 24 h and then treated with or without 1 μm OA for 2 h. Cell lysates were incubated with anti-GFP antibody, and the immunoprecipitates were analyzed by immunoblotting with <t>anti-phospho-MAPK/CDK</t> substrates and anti-GFP antibodies.
    Sc 126 Santa Cruz Biotechnology Pdi Adi Spa 890 Enzo Life Sciences Phospho Mapk Cdk Substrates, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sc 126 santa cruz biotechnology pdi adi spa 890 enzo life sciences phospho mapk cdk substrates/product/Enzo Biochem
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sc 126 santa cruz biotechnology pdi adi spa 890 enzo life sciences phospho mapk cdk substrates - by Bioz Stars, 2024-09
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    Identification of the phosphorylation site of Par3 by ERK2. A, The direct phosphorylation of Par3 by ERK2. Purified MBP or MBP-Par3 deletion mutants were incubated with recombinant ERK2 in the presence of [γ-32P]ATP in vitro. Samples were subjected to SDS-PAGE and silver staining (bottom) followed by autoradiography (top). Asterisks indicate intact MBP-fusion proteins. B, The phosphorylation of Par3 point mutants by ERK2. Purified MBP, MBP-Par3-4N-WT -S1116A, -T1145A, -S1316A, or -T1328A was incubated with recombinant ERK2 in the presence of [γ-32P]ATP in vitro. Samples were subjected to SDS-PAGE and silver staining (bottom) followed by autoradiography (top). Asterisks indicate intact MBP-fusion proteins. C, The alignment of ERK2 phosphorylation sites in Par3 homologs (rat, mouse, human). D, The phosphorylation of Par3-WT or -S1116A in COS7 cells. COS7 cells were transfected with EGFP-Par3-WT or -S1116A and cultured for 24 h. The cells were serum-starved for 24 h and then treated with or without 1 μm OA for 2 h. Cell lysates were incubated with anti-GFP antibody, and the immunoprecipitates were analyzed by immunoblotting with anti-phospho-MAPK/CDK substrates and anti-GFP antibodies.

    Journal: The Journal of Neuroscience

    Article Title: ERK2-Mediated Phosphorylation of Par3 Regulates Neuronal Polarization

    doi: 10.1523/JNEUROSCI.4210-12.2013

    Figure Lengend Snippet: Identification of the phosphorylation site of Par3 by ERK2. A, The direct phosphorylation of Par3 by ERK2. Purified MBP or MBP-Par3 deletion mutants were incubated with recombinant ERK2 in the presence of [γ-32P]ATP in vitro. Samples were subjected to SDS-PAGE and silver staining (bottom) followed by autoradiography (top). Asterisks indicate intact MBP-fusion proteins. B, The phosphorylation of Par3 point mutants by ERK2. Purified MBP, MBP-Par3-4N-WT -S1116A, -T1145A, -S1316A, or -T1328A was incubated with recombinant ERK2 in the presence of [γ-32P]ATP in vitro. Samples were subjected to SDS-PAGE and silver staining (bottom) followed by autoradiography (top). Asterisks indicate intact MBP-fusion proteins. C, The alignment of ERK2 phosphorylation sites in Par3 homologs (rat, mouse, human). D, The phosphorylation of Par3-WT or -S1116A in COS7 cells. COS7 cells were transfected with EGFP-Par3-WT or -S1116A and cultured for 24 h. The cells were serum-starved for 24 h and then treated with or without 1 μm OA for 2 h. Cell lysates were incubated with anti-GFP antibody, and the immunoprecipitates were analyzed by immunoblotting with anti-phospho-MAPK/CDK substrates and anti-GFP antibodies.

    Article Snippet: The following antibodies and materials were used: polyclonal rabbit anti-Par3 antibody raised against Par3-4N/3S-GST ( Nishimura et al., 2004 ); polyclonal rabbit anti-Par3 and monoclonal mouse anti-Cdk5 antibodies (Millipore); polyclonal rabbit anti-c-Myc antibody (Santa Cruz Biotechnology); polyclonal rabbit anti-phospho-ERK1/2 (T202/Y204), polyclonal rabbit anti-ERK1/2, monoclonal rabbit anti-phospho-MAPK/CDK substrates (PXSP or SPXR/K) (34B2), monoclonal mouse anti-JNK1 (C26), and monoclonal mouse anti-p38α MAPK antibodies (Cell Signaling Technology); monoclonal mouse anti-ERK2, monoclonal mouse anti-KIF3A, and monoclonal mouse anti-GSK-3β antibodies (BD Biosciences); monoclonal mouse anti-KIF3A (K2.4) antibody (Abcam); monoclonal mouse anti-Myc (9E10), monoclonal mouse anti-α-tubulin (DM1A), monoclonal mouse anti-GST, and monoclonal mouse anti-Flag antibodies (Sigma); monoclonal mouse anti-Tau-1 (Millipore Bioscience Research Reagents); monoclonal mouse anti-class III β-tubulin and polyclonal rabbit anti-class III β-tubulin antibodies (Tuj1, Covance); polyclonal rabbit anti-GFP and polyclonal rabbit anti-HA antibodies (MBL); and monoclonal mouse anti-GFP antibody (Roche Diagnostics).

    Techniques: Purification, Incubation, Recombinant, In Vitro, SDS Page, Silver Staining, Autoradiography, Transfection, Cell Culture, Western Blot