anti p75 ngf receptor intracellular antibody Search Results


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  • 93
    Alomone Labs anti p75 ngf receptor intracellular antibody
    Anti P75 Ngf Receptor Intracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p75 ngf receptor intracellular antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p75 ngf receptor intracellular antibody - by Bioz Stars, 2022-08
    93/100 stars
      Buy from Supplier

    93
    Abcam anti p75ntr intracellular region
    <t>p75NTR</t> activation induces SREBP2 cleavage in a caspase-3-dependent manner. A, schematic structure of SREBP2 in the membrane, its interaction with the SCAP·INSIG complex, and the protease cleavage sites. In addition to the classical S1P and S2P
    Anti P75ntr Intracellular Region, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p75ntr intracellular region/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p75ntr intracellular region - by Bioz Stars, 2022-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    p75NTR activation induces SREBP2 cleavage in a caspase-3-dependent manner. A, schematic structure of SREBP2 in the membrane, its interaction with the SCAP·INSIG complex, and the protease cleavage sites. In addition to the classical S1P and S2P

    Journal: The Journal of Biological Chemistry

    Article Title: p75 Neurotrophin Receptor Signaling Activates Sterol Regulatory Element-binding Protein-2 in Hepatocyte Cells via p38 Mitogen-activated Protein Kinase and Caspase-3 *

    doi: 10.1074/jbc.M116.722272

    Figure Lengend Snippet: p75NTR activation induces SREBP2 cleavage in a caspase-3-dependent manner. A, schematic structure of SREBP2 in the membrane, its interaction with the SCAP·INSIG complex, and the protease cleavage sites. In addition to the classical S1P and S2P

    Article Snippet: The filters were incubated for 1 h in 50 m m Tris-HCl, pH 7.5, 150 m m NaCl, supplemented with 0.1% Tween 20, and 5% skimmed milk or 5% BSA at room temperature and then overnight at 4 °C with primary antibodies as follows: anti-p75NTR intracellular region (1:1000, Abcam, ab32888); anti-p75NTR extracellular region (1:5000, Millipore, 1554); anti-caspase-3 (1:1000, Cell Signaling, 9665); anti-caspase-3 cleaved form (1:1000, Cell Signaling, 9661); anti-caspase-2 (1:500, Enzo Life Sciences, 11B4); anti-SREBP carboxyl-terminal region (1:500, BD Biosciences, 557037); anti-SREBP amino-terminal region (1:1500, Abcam, ab3062); anti-LDLR (1:2000; Cayman Chemical, 10007665); anti-p38 MAPK (1:1000, Cell Signaling, 9212); anti-phospho-p38 MAPK (1:1000, Cell Signaling, 9216); anti-histone H3 (1:1000, Cell Signaling, 9715); rabbit anti-GFP (1:500, Chemicon); mouse anti-GFP (1:5000, Roche Applied Science); mouse anti-binding immunoglobulin protein (1:1000; BD Biosciences); and anti-β-actin (1:5000; Sigma).

    Techniques: Activation Assay

    Schematic summary of the results. Stimulation of p75NTR with pro-NGF or NGF activates p38 MAPK signaling in the hepatocytes resulting in caspase-3 activation in the membrane fraction. Caspase-3 in turn cleaves SREBP2 with the subsequent release of the

    Journal: The Journal of Biological Chemistry

    Article Title: p75 Neurotrophin Receptor Signaling Activates Sterol Regulatory Element-binding Protein-2 in Hepatocyte Cells via p38 Mitogen-activated Protein Kinase and Caspase-3 *

    doi: 10.1074/jbc.M116.722272

    Figure Lengend Snippet: Schematic summary of the results. Stimulation of p75NTR with pro-NGF or NGF activates p38 MAPK signaling in the hepatocytes resulting in caspase-3 activation in the membrane fraction. Caspase-3 in turn cleaves SREBP2 with the subsequent release of the

    Article Snippet: The filters were incubated for 1 h in 50 m m Tris-HCl, pH 7.5, 150 m m NaCl, supplemented with 0.1% Tween 20, and 5% skimmed milk or 5% BSA at room temperature and then overnight at 4 °C with primary antibodies as follows: anti-p75NTR intracellular region (1:1000, Abcam, ab32888); anti-p75NTR extracellular region (1:5000, Millipore, 1554); anti-caspase-3 (1:1000, Cell Signaling, 9665); anti-caspase-3 cleaved form (1:1000, Cell Signaling, 9661); anti-caspase-2 (1:500, Enzo Life Sciences, 11B4); anti-SREBP carboxyl-terminal region (1:500, BD Biosciences, 557037); anti-SREBP amino-terminal region (1:1500, Abcam, ab3062); anti-LDLR (1:2000; Cayman Chemical, 10007665); anti-p38 MAPK (1:1000, Cell Signaling, 9212); anti-phospho-p38 MAPK (1:1000, Cell Signaling, 9216); anti-histone H3 (1:1000, Cell Signaling, 9715); rabbit anti-GFP (1:500, Chemicon); mouse anti-GFP (1:5000, Roche Applied Science); mouse anti-binding immunoglobulin protein (1:1000; BD Biosciences); and anti-β-actin (1:5000; Sigma).

    Techniques: Activation Assay

    p75NTR activation increases caspase-3 activity in the membrane fraction. Huh7 cells were stimulated with 5 ng/ml pro-NGF or 50 ng/ml NGF for 16 h followed by cell isolation of cytosolic and membrane fractions. Caspase 3/7 activity was determined as described

    Journal: The Journal of Biological Chemistry

    Article Title: p75 Neurotrophin Receptor Signaling Activates Sterol Regulatory Element-binding Protein-2 in Hepatocyte Cells via p38 Mitogen-activated Protein Kinase and Caspase-3 *

    doi: 10.1074/jbc.M116.722272

    Figure Lengend Snippet: p75NTR activation increases caspase-3 activity in the membrane fraction. Huh7 cells were stimulated with 5 ng/ml pro-NGF or 50 ng/ml NGF for 16 h followed by cell isolation of cytosolic and membrane fractions. Caspase 3/7 activity was determined as described

    Article Snippet: The filters were incubated for 1 h in 50 m m Tris-HCl, pH 7.5, 150 m m NaCl, supplemented with 0.1% Tween 20, and 5% skimmed milk or 5% BSA at room temperature and then overnight at 4 °C with primary antibodies as follows: anti-p75NTR intracellular region (1:1000, Abcam, ab32888); anti-p75NTR extracellular region (1:5000, Millipore, 1554); anti-caspase-3 (1:1000, Cell Signaling, 9665); anti-caspase-3 cleaved form (1:1000, Cell Signaling, 9661); anti-caspase-2 (1:500, Enzo Life Sciences, 11B4); anti-SREBP carboxyl-terminal region (1:500, BD Biosciences, 557037); anti-SREBP amino-terminal region (1:1500, Abcam, ab3062); anti-LDLR (1:2000; Cayman Chemical, 10007665); anti-p38 MAPK (1:1000, Cell Signaling, 9212); anti-phospho-p38 MAPK (1:1000, Cell Signaling, 9216); anti-histone H3 (1:1000, Cell Signaling, 9715); rabbit anti-GFP (1:500, Chemicon); mouse anti-GFP (1:5000, Roche Applied Science); mouse anti-binding immunoglobulin protein (1:1000; BD Biosciences); and anti-β-actin (1:5000; Sigma).

    Techniques: Activation Assay, Activity Assay, Cell Isolation

    Involvement of pro-NGF and p75NTR in LDLR regulation in hepatocyte cells experiments using Huh7 hepatocytes ( A–D ) and primary mouse hepatocytes ( E ). Values are given mean ± S.E. A, p75NTR is expressed by Huh7 cells as shown by immunoblotting

    Journal: The Journal of Biological Chemistry

    Article Title: p75 Neurotrophin Receptor Signaling Activates Sterol Regulatory Element-binding Protein-2 in Hepatocyte Cells via p38 Mitogen-activated Protein Kinase and Caspase-3 *

    doi: 10.1074/jbc.M116.722272

    Figure Lengend Snippet: Involvement of pro-NGF and p75NTR in LDLR regulation in hepatocyte cells experiments using Huh7 hepatocytes ( A–D ) and primary mouse hepatocytes ( E ). Values are given mean ± S.E. A, p75NTR is expressed by Huh7 cells as shown by immunoblotting

    Article Snippet: The filters were incubated for 1 h in 50 m m Tris-HCl, pH 7.5, 150 m m NaCl, supplemented with 0.1% Tween 20, and 5% skimmed milk or 5% BSA at room temperature and then overnight at 4 °C with primary antibodies as follows: anti-p75NTR intracellular region (1:1000, Abcam, ab32888); anti-p75NTR extracellular region (1:5000, Millipore, 1554); anti-caspase-3 (1:1000, Cell Signaling, 9665); anti-caspase-3 cleaved form (1:1000, Cell Signaling, 9661); anti-caspase-2 (1:500, Enzo Life Sciences, 11B4); anti-SREBP carboxyl-terminal region (1:500, BD Biosciences, 557037); anti-SREBP amino-terminal region (1:1500, Abcam, ab3062); anti-LDLR (1:2000; Cayman Chemical, 10007665); anti-p38 MAPK (1:1000, Cell Signaling, 9212); anti-phospho-p38 MAPK (1:1000, Cell Signaling, 9216); anti-histone H3 (1:1000, Cell Signaling, 9715); rabbit anti-GFP (1:500, Chemicon); mouse anti-GFP (1:5000, Roche Applied Science); mouse anti-binding immunoglobulin protein (1:1000; BD Biosciences); and anti-β-actin (1:5000; Sigma).

    Techniques: