anti p21 anti body Search Results


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  • 96
    Santa Cruz Biotechnology p21
    P21, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p21/product/Santa Cruz Biotechnology
    Average 96 stars, based on 1 article reviews
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    p21 - by Bioz Stars, 2024-06
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    96
    Cell Signaling Technology Inc anti p21
    Anti P21, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p21/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
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    anti p21 - by Bioz Stars, 2024-06
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    93
    Proteintech immunostaining pak4
    Immunostaining Pak4, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunostaining pak4/product/Proteintech
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    96
    Proteintech p21
    a Representative MRIs of patients with Pfirrmann grade I, III, and V disc degeneration. White arrow: the operated segment. b The mRNA levels of ECM markers in NPCs extracted from different NP tissues ( n = 3, three different donors for three independent experiments; *: versus grade I, P < 0.05, ** P < 0.01). c The protein levels of ECM markers in NPCs from tissues of different Pfirrmann grades ( n = 3, three different donors). d Representative images of safranin staining and alcian blue staining of different NP tissues ( n = 5, five different donors; scale bar: 20 μm). e p53, <t>p21,</t> and p16 expression in different NP tissues was visualized by immunofluorescence staining ( n = 5, five different donors; scale bar: 100 μm). f The ROS level in NPCs was evaluated using a DCFH-DA staining assay ( n = 5, five different donors; scale bar: 25 μm). All data are presented as the mean ± SD values.
    P21, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p21/product/Proteintech
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p21 - by Bioz Stars, 2024-06
    96/100 stars
      Buy from Supplier

    96
    Proteintech monoclonal antibody anti p21
    a Representative MRIs of patients with Pfirrmann grade I, III, and V disc degeneration. White arrow: the operated segment. b The mRNA levels of ECM markers in NPCs extracted from different NP tissues ( n = 3, three different donors for three independent experiments; *: versus grade I, P < 0.05, ** P < 0.01). c The protein levels of ECM markers in NPCs from tissues of different Pfirrmann grades ( n = 3, three different donors). d Representative images of safranin staining and alcian blue staining of different NP tissues ( n = 5, five different donors; scale bar: 20 μm). e p53, <t>p21,</t> and p16 expression in different NP tissues was visualized by immunofluorescence staining ( n = 5, five different donors; scale bar: 100 μm). f The ROS level in NPCs was evaluated using a DCFH-DA staining assay ( n = 5, five different donors; scale bar: 25 μm). All data are presented as the mean ± SD values.
    Monoclonal Antibody Anti P21, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal antibody anti p21/product/Proteintech
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal antibody anti p21 - by Bioz Stars, 2024-06
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    Image Search Results


    a Representative MRIs of patients with Pfirrmann grade I, III, and V disc degeneration. White arrow: the operated segment. b The mRNA levels of ECM markers in NPCs extracted from different NP tissues ( n = 3, three different donors for three independent experiments; *: versus grade I, P < 0.05, ** P < 0.01). c The protein levels of ECM markers in NPCs from tissues of different Pfirrmann grades ( n = 3, three different donors). d Representative images of safranin staining and alcian blue staining of different NP tissues ( n = 5, five different donors; scale bar: 20 μm). e p53, p21, and p16 expression in different NP tissues was visualized by immunofluorescence staining ( n = 5, five different donors; scale bar: 100 μm). f The ROS level in NPCs was evaluated using a DCFH-DA staining assay ( n = 5, five different donors; scale bar: 25 μm). All data are presented as the mean ± SD values.

    Journal: Experimental & Molecular Medicine

    Article Title: Oxidative stress-induced circKIF18A downregulation impairs MCM7-mediated anti-senescence in intervertebral disc degeneration

    doi: 10.1038/s12276-022-00732-0

    Figure Lengend Snippet: a Representative MRIs of patients with Pfirrmann grade I, III, and V disc degeneration. White arrow: the operated segment. b The mRNA levels of ECM markers in NPCs extracted from different NP tissues ( n = 3, three different donors for three independent experiments; *: versus grade I, P < 0.05, ** P < 0.01). c The protein levels of ECM markers in NPCs from tissues of different Pfirrmann grades ( n = 3, three different donors). d Representative images of safranin staining and alcian blue staining of different NP tissues ( n = 5, five different donors; scale bar: 20 μm). e p53, p21, and p16 expression in different NP tissues was visualized by immunofluorescence staining ( n = 5, five different donors; scale bar: 100 μm). f The ROS level in NPCs was evaluated using a DCFH-DA staining assay ( n = 5, five different donors; scale bar: 25 μm). All data are presented as the mean ± SD values.

    Article Snippet: The membranes were blocked with 5% skim milk powder in TBST for 2 h at room temperature and incubated with antibodies specific for β-actin (AF5001, Beyotime), MCM7 (A11325, Abclonal, Wuhan, China), p21 (27296-1-AP, Proteintech, Wuhan, China), p16 (A0262, Abclonal), aggrecan (13880-1-AP, Proteintech), collagen II (28459-1-AP, Proteintech), MMP-13 (18165-1-AP, Proteintech), and MMP-3 (17873-1-AP, Proteintech) overnight at 4 °C.

    Techniques: Staining, Expressing, Immunofluorescence

    a The CCK-8 assay showed the effects of circKIF18A and sh-MCM7 on NPC viability ( n = 3, three different donors; * P < 0.05; ** P < 0.01). b The EdU assay revealed that MCM7 mediated the effects of circKIF18A on proliferation in H 2 O 2 -treated NPCs ( n = 5, five different donors; scale bar: 50 μm). c and d SA-β-gal staining assay of NPCs transfected with or without circKIF18A and sh-MCM7 lentiviruses under oxidative stress ( n = 3, three different donors for three individual experiments; * P < 0.05, ** P < 0.01; scale bar: 50 μm). e and f MCM7 knockdown promoted G0/G1 arrest, which was rescued by circKIF18A overexpression, in NPCs under oxidative stress ( n = 3, three different donors; * P < 0.05; ** P < 0.01). g The protein levels of aggrecan, collagen II, MMP-13, and MMP-3 in NPCs transfected with circKIF18A, sh-MCM7, and empty lentiviruses were determined using western blotting (n = 3, three different donors). h The qRT-PCR assay showed the effects of MCM7 on the mRNA expression of ACAN , COL2A1 , MMP13 and MMP3 in circKIF18A-overexpressing NPCs ( n = 3, three different donors for three individual experiments; NS, no significance; * P < 0.05; ** P < 0.01). i The protein levels of p53, p21, and p16 were measured using western blotting. NPCs were stably transfected with circKIF18A, sh-MCM7, and empty lentiviruses ( n = 3, three different donors). j Western blot assays showed the effects of MCM7 on the protein levels of cyclin D1, CDK4, p-RB and RB in NPCs treated as described above ( n = 3, three different donors). k The effect of p53 knockdown on the p21 level in sh-circKIF18A NPCs ( n = 3, three different donors). l and m Representative images of the DCFH-DA staining assay in NPCs with circKIF18A overexpression or MCM7 knockdown under oxidative stress ( n = 5, five different donors; NS no significance. Scale bar: 20 μm). All data are presented as the mean ± SD values.

    Journal: Experimental & Molecular Medicine

    Article Title: Oxidative stress-induced circKIF18A downregulation impairs MCM7-mediated anti-senescence in intervertebral disc degeneration

    doi: 10.1038/s12276-022-00732-0

    Figure Lengend Snippet: a The CCK-8 assay showed the effects of circKIF18A and sh-MCM7 on NPC viability ( n = 3, three different donors; * P < 0.05; ** P < 0.01). b The EdU assay revealed that MCM7 mediated the effects of circKIF18A on proliferation in H 2 O 2 -treated NPCs ( n = 5, five different donors; scale bar: 50 μm). c and d SA-β-gal staining assay of NPCs transfected with or without circKIF18A and sh-MCM7 lentiviruses under oxidative stress ( n = 3, three different donors for three individual experiments; * P < 0.05, ** P < 0.01; scale bar: 50 μm). e and f MCM7 knockdown promoted G0/G1 arrest, which was rescued by circKIF18A overexpression, in NPCs under oxidative stress ( n = 3, three different donors; * P < 0.05; ** P < 0.01). g The protein levels of aggrecan, collagen II, MMP-13, and MMP-3 in NPCs transfected with circKIF18A, sh-MCM7, and empty lentiviruses were determined using western blotting (n = 3, three different donors). h The qRT-PCR assay showed the effects of MCM7 on the mRNA expression of ACAN , COL2A1 , MMP13 and MMP3 in circKIF18A-overexpressing NPCs ( n = 3, three different donors for three individual experiments; NS, no significance; * P < 0.05; ** P < 0.01). i The protein levels of p53, p21, and p16 were measured using western blotting. NPCs were stably transfected with circKIF18A, sh-MCM7, and empty lentiviruses ( n = 3, three different donors). j Western blot assays showed the effects of MCM7 on the protein levels of cyclin D1, CDK4, p-RB and RB in NPCs treated as described above ( n = 3, three different donors). k The effect of p53 knockdown on the p21 level in sh-circKIF18A NPCs ( n = 3, three different donors). l and m Representative images of the DCFH-DA staining assay in NPCs with circKIF18A overexpression or MCM7 knockdown under oxidative stress ( n = 5, five different donors; NS no significance. Scale bar: 20 μm). All data are presented as the mean ± SD values.

    Article Snippet: The membranes were blocked with 5% skim milk powder in TBST for 2 h at room temperature and incubated with antibodies specific for β-actin (AF5001, Beyotime), MCM7 (A11325, Abclonal, Wuhan, China), p21 (27296-1-AP, Proteintech, Wuhan, China), p16 (A0262, Abclonal), aggrecan (13880-1-AP, Proteintech), collagen II (28459-1-AP, Proteintech), MMP-13 (18165-1-AP, Proteintech), and MMP-3 (17873-1-AP, Proteintech) overnight at 4 °C.

    Techniques: CCK-8 Assay, EdU Assay, Staining, Transfection, Over Expression, Western Blot, Quantitative RT-PCR, Expressing, Stable Transfection

    a S&O staining revealed the effects of circKIF18A overexpression adenovirus treatment on IVDD in vivo ( n = 9; scale bar: 1000 μm). b Alcian blue staining revealed the effects of circKIF18A overexpression adenovirus treatment on IVDD in rats ( n = 9; scale bar: 1000 μm). c The effects of circKIF18A overexpression adenovirus treatment on disc height in rats were determined using X-ray imaging ( n = 9). d The statistical graph of histological scores in different groups according to S&O staining ( n = 9; * P < 0.05; ** P < 0.01). e The statistical graph of the disc height index in different groups according to X-ray imaging ( n = 9; * P < 0.05; ** P < 0.01). f The effects of circKIF18A on ECM metabolism in rat IVDs ( n = 5; scale bar: 50 μm). g CircKIF18A overexpression adenovirus treatment increased the p53 level in NPs ( n = 5; scale bar: 50 μm). h Representative images of immunofluorescence staining for p21 in punctured NPs ( n = 5; scale bar: 50 μm). i Representative images of immunofluorescence staining for p16 in punctured NPs ( n = 5; scale bar: 50 μm). All data are presented as the mean ± SD values.

    Journal: Experimental & Molecular Medicine

    Article Title: Oxidative stress-induced circKIF18A downregulation impairs MCM7-mediated anti-senescence in intervertebral disc degeneration

    doi: 10.1038/s12276-022-00732-0

    Figure Lengend Snippet: a S&O staining revealed the effects of circKIF18A overexpression adenovirus treatment on IVDD in vivo ( n = 9; scale bar: 1000 μm). b Alcian blue staining revealed the effects of circKIF18A overexpression adenovirus treatment on IVDD in rats ( n = 9; scale bar: 1000 μm). c The effects of circKIF18A overexpression adenovirus treatment on disc height in rats were determined using X-ray imaging ( n = 9). d The statistical graph of histological scores in different groups according to S&O staining ( n = 9; * P < 0.05; ** P < 0.01). e The statistical graph of the disc height index in different groups according to X-ray imaging ( n = 9; * P < 0.05; ** P < 0.01). f The effects of circKIF18A on ECM metabolism in rat IVDs ( n = 5; scale bar: 50 μm). g CircKIF18A overexpression adenovirus treatment increased the p53 level in NPs ( n = 5; scale bar: 50 μm). h Representative images of immunofluorescence staining for p21 in punctured NPs ( n = 5; scale bar: 50 μm). i Representative images of immunofluorescence staining for p16 in punctured NPs ( n = 5; scale bar: 50 μm). All data are presented as the mean ± SD values.

    Article Snippet: The membranes were blocked with 5% skim milk powder in TBST for 2 h at room temperature and incubated with antibodies specific for β-actin (AF5001, Beyotime), MCM7 (A11325, Abclonal, Wuhan, China), p21 (27296-1-AP, Proteintech, Wuhan, China), p16 (A0262, Abclonal), aggrecan (13880-1-AP, Proteintech), collagen II (28459-1-AP, Proteintech), MMP-13 (18165-1-AP, Proteintech), and MMP-3 (17873-1-AP, Proteintech) overnight at 4 °C.

    Techniques: Staining, Over Expression, In Vivo, Imaging, Immunofluorescence