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Image Search Results
Journal: Scientific Reports
Article Title: AMP-activated protein kinase modulates tau phosphorylation and tau pathology in vivo
doi: 10.1038/srep26758
Figure Lengend Snippet: ( a,b) Immunofluorescence staining of total AMPK (AMPKα1/α2, green) and MAP2 (red) ( a ) or of activated AMPK (p-Thr 172 AMPK, green) and total tau (Tau5, red) ( b ) in primary neurons at 15 DIV. ( c ) PLA in primary neurons at 15 DIV using activated AMPK (p-Thr 172 AMPK) and total tau (Tau5) primary antibodies. PLA negative control (top panel). Green spots indicate interactions between AMPK and tau (bottom panel). Scale bars = 50 μm.
Article Snippet: Anti p-Thr 172 AMPK antibody and
Techniques: Immunofluorescence, Staining, Negative Control
Journal: Scientific Reports
Article Title: AMP-activated protein kinase modulates tau phosphorylation and tau pathology in vivo
doi: 10.1038/srep26758
Figure Lengend Snippet: Primary neurons at 15 DIV were treated for the indicated times with the AMPK activator AICAR (1 mM). AMPK and ACC expression and activation were monitored by Western-blot (WB) using antibodies against AMPK, p-Thr 172 AMPK (pAMPK), ACC, p-Ser 79 ACC (pACC) and actin ( a ). Quantifications of the ratios AMPK/actin ( b ) pAMPK/AMPK ( c ) and pACC/ACC ( d ). WB analysis ( e ) and quantification of total tau ( f ) and phosphorylated tau at epitopes Ser 262/356 ( g ), Thr 231 ( h ), Ser 396/404 ( i ) and Ser 202 ( j ) expressed in ratios. Cytotoxicity assay following 6 h and 24 h AICAR treatments in primary neurons at 15 DIV determined using the lactate dehydrogenase (LDH) test ( k ). Treatment with 0.9% Triton X-100 was used as a positive control. Results represent mean ± SD, n = 4–6. a.u., arbitrary units. *p < 0.05, **p < 0.01, ***p < 0.001 compared to Ctrl, One way ANOVA with Bonferroni’s post hoc test.
Article Snippet: Anti p-Thr 172 AMPK antibody and
Techniques: Expressing, Activation Assay, Western Blot, Cytotoxicity Assay, Positive Control