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Image Search Results

Journal: Frontiers in Cellular Neuroscience
Article Title: Distinct Residential and Infiltrated Macrophage Populations and Their Phagocytic Function in Mild and Severe Neonatal Hypoxic-Ischemic Brain Damage
doi: 10.3389/fncel.2020.00244
Figure Lengend Snippet: The expression of toll-like receptor 2 (TLR2) and lysosome-associated membrane protein 1 (LAMP1) on CD11b + CD45 low-med and CD11b + CD45 high cells in mild and severe brain injury after HI. (A) Percentage of TLR2 + /CD11b + CD45 low-med fluorescence intensity at 1 day and 3 days after HI in the C group, HI-M group, and HI-S group. (B) The percentage of TLR2 + /CD11b + CD45 high fluorescence intensity at 1 day and 3 days after HI. (C) Quantification of TLR2 + /CD11b + CD45 low-med cells at 1 and 3 days after HI. (D) Quantification of TLR2 + /CD11b + CD45 high cells at 1 and 3 days after HI. (E) The percentage of LAMP1 + /CD11b + CD45 low-med fluorescence intensity at 1 day and 3 days after HI in the C group, HI-M group, and HI-S group. (F) The percentage of LAMP1 + /CD11b + CD45 high fluorescence intensity at 1 day and 3 days after HI. (G) Quantification of LAMP1 + /CD11b + CD45 low-med cells at 1 and 3 days after HI. (H) Quantification of LAMP1 + /CD11b + CD45 high cells at 1 and 3 days after HI. C, control group; HI-M, hypoxia-ischemia brain damage with mild injury; HI-S, hypoxia-ischemia brain damage with severe injury. * p < 0.05 HI-M vs. C, ** p < 0.01 HI-M vs. C, # p < 0.05 HI-S vs. C, △ p < 0.05 HI-S vs. HI-M, **** p < 0.0001 HI-M vs. C, # p < 0.05 HI-S vs. C, △ p < 0.05 HI-S vs. HI-M, n = 4–6 animals per group, data expressed as the means ± SEM.
Article Snippet: The following combinations of antibodies with a 1:100 dilution in staining buffer were used: (1) APC anti-mouse CD45 (Biolegend)/FITC anti-mouse CD11b (Biolegend)/PE anti-mouse CD36 (Biolegend)/PerCP anti-mouse LAMP1 (Nouvs); and (2) APC anti-mouse CD45 (Biolegend)/FITC anti-mouse CD11b (Biolegend)/PE
Techniques: Expressing, Fluorescence, Hi-C