anti mouse igg1 gamma chain specific biotin antibody Search Results


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  • 96
    Vector Laboratories biotinylated goat anti mouse ab igg
    Biotinylated Goat Anti Mouse Ab Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore anti rabbit igg gamma chain specific biotin antibody mouse monoclonal
    Anti Rabbit Igg Gamma Chain Specific Biotin Antibody Mouse Monoclonal, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 448 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore anti mouse igg1 gamma chain specific biotin antibody
    Anti Mouse Igg1 Gamma Chain Specific Biotin Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SouthernBiotech g chain specific biotin goat anti mouse igg
    G Chain Specific Biotin Goat Anti Mouse Igg, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp igg fraction monoclonal mouse anti rabbit igg light chain specific
    Biotin Sp Igg Fraction Monoclonal Mouse Anti Rabbit Igg Light Chain Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore anti mouse igg gamma chain specific biotin antibody produced in goat
    Anti Mouse Igg Gamma Chain Specific Biotin Antibody Produced In Goat, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    thermo fisher anti mouse igg gamma chain specific biotin antibody produced in goat
    Anti Mouse Igg Gamma Chain Specific Biotin Antibody Produced In Goat, supplied by thermo fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bio-Rad biotin labeled rat anti mouse igg2a heavy chain specific antibody
    The production of IgG1 and <t>IgG2a</t> by OVA-immunized BALB/c mice injected with OdDHL. Serum from OVA-immunized BALB/c mice, given either an OdDHL or control injection, was collected, and an ELISA was used to quantify OVA-specific IgG1 (A) and IgG2a (B) levels. Data are represented as the median ± 25th and 75th percentiles (boxes). Error bars represent the maximum and minimum values; n = 7 to 10. *, P
    Biotin Labeled Rat Anti Mouse Igg2a Heavy Chain Specific Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher biotinylated anti mouse γ chain specific igg antibody
    The production of IgG1 and <t>IgG2a</t> by OVA-immunized BALB/c mice injected with OdDHL. Serum from OVA-immunized BALB/c mice, given either an OdDHL or control injection, was collected, and an ELISA was used to quantify OVA-specific IgG1 (A) and IgG2a (B) levels. Data are represented as the median ± 25th and 75th percentiles (boxes). Error bars represent the maximum and minimum values; n = 7 to 10. *, P
    Biotinylated Anti Mouse γ Chain Specific Igg Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti mouse igg
    The production of IgG1 and <t>IgG2a</t> by OVA-immunized BALB/c mice injected with OdDHL. Serum from OVA-immunized BALB/c mice, given either an OdDHL or control injection, was collected, and an ELISA was used to quantify OVA-specific IgG1 (A) and IgG2a (B) levels. Data are represented as the median ± 25th and 75th percentiles (boxes). Error bars represent the maximum and minimum values; n = 7 to 10. *, P
    Biotin Sp Affinipure Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 91/100, based on 223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore anti human igg gamma chain specific biotin antibody
    The production of IgG1 and <t>IgG2a</t> by OVA-immunized BALB/c mice injected with OdDHL. Serum from OVA-immunized BALB/c mice, given either an OdDHL or control injection, was collected, and an ELISA was used to quantify OVA-specific IgG1 (A) and IgG2a (B) levels. Data are represented as the median ± 25th and 75th percentiles (boxes). Error bars represent the maximum and minimum values; n = 7 to 10. *, P
    Anti Human Igg Gamma Chain Specific Biotin Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp conjugated affinipure goat anti mouse igg light chain specific antibody
    The production of IgG1 and <t>IgG2a</t> by OVA-immunized BALB/c mice injected with OdDHL. Serum from OVA-immunized BALB/c mice, given either an OdDHL or control injection, was collected, and an ELISA was used to quantify OVA-specific IgG1 (A) and IgG2a (B) levels. Data are represented as the median ± 25th and 75th percentiles (boxes). Error bars represent the maximum and minimum values; n = 7 to 10. *, P
    Biotin Sp Conjugated Affinipure Goat Anti Mouse Igg Light Chain Specific Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SouthernBiotech heavy chain specific goat anti mouse igg
    The production of IgG1 and <t>IgG2a</t> by OVA-immunized BALB/c mice injected with OdDHL. Serum from OVA-immunized BALB/c mice, given either an OdDHL or control injection, was collected, and an ELISA was used to quantify OVA-specific IgG1 (A) and IgG2a (B) levels. Data are represented as the median ± 25th and 75th percentiles (boxes). Error bars represent the maximum and minimum values; n = 7 to 10. *, P
    Heavy Chain Specific Goat Anti Mouse Igg, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Pharmingen biotin conjugated anti mouse igg1
    Effect of Stat6 deficiency on serum OVA-specific <t>IgG1</t> ( A ) and OVA-specific IgG2a ( B ) antibody levels of mice 3 d after a single antigen or PBS aspiration challenge. Mice were treated as described in Fig. 1 . Serum was diluted 1:100, 1: 250, and 1:500 with FBS for analysis of OVA-specific IgG1 and IgG2a antibody levels by ELISA. Since recombinant OVA-specific antibodies were not available to generate a standard curve, relative antibody levels are reported using OD readings obtained after background absorbance was subtracted. Reported OD values are from samples diluted 1:250, all of which were below the saturation point of the assay as demonstrated by comparison to OD values obtained from samples diluted 1:100. Values are reported as mean and SE ( n = 9). * P value
    Biotin Conjugated Anti Mouse Igg1, supplied by Pharmingen, used in various techniques. Bioz Stars score: 89/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore biotinylated chain specific goat anti mouse igg
    Effect of Stat6 deficiency on serum OVA-specific <t>IgG1</t> ( A ) and OVA-specific IgG2a ( B ) antibody levels of mice 3 d after a single antigen or PBS aspiration challenge. Mice were treated as described in Fig. 1 . Serum was diluted 1:100, 1: 250, and 1:500 with FBS for analysis of OVA-specific IgG1 and IgG2a antibody levels by ELISA. Since recombinant OVA-specific antibodies were not available to generate a standard curve, relative antibody levels are reported using OD readings obtained after background absorbance was subtracted. Reported OD values are from samples diluted 1:250, all of which were below the saturation point of the assay as demonstrated by comparison to OD values obtained from samples diluted 1:100. Values are reported as mean and SE ( n = 9). * P value
    Biotinylated Chain Specific Goat Anti Mouse Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SouthernBiotech biotin conjugated goat anti mouse igg ab
    Glomerular immune deposits do not require anti-DNA antibodies. (A) Glomerular immune deposits were detected by direct immunofluorescence for <t>IgG</t> (top) and complement C3 (bottom) in frozen kidney sections from <t>TLR9</t> +/+ (left), TLR9 −/− (middle), and nonautoimmune C57BL/6 control mice (right). Representative images are shown. Original magnification, 400. (B) Mean glomerular fluorescence intensity (arbitrary units) was determined for IgG and C3 in TLR9 +/+ ( n = 8) and TLR9 −/− ( n = 8) mice. Horizontal lines represent mean values.
    Biotin Conjugated Goat Anti Mouse Igg Ab, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SouthernBiotech biotin labeled goat anti mouse igg
    Glomerular immune deposits do not require anti-DNA antibodies. (A) Glomerular immune deposits were detected by direct immunofluorescence for <t>IgG</t> (top) and complement C3 (bottom) in frozen kidney sections from <t>TLR9</t> +/+ (left), TLR9 −/− (middle), and nonautoimmune C57BL/6 control mice (right). Representative images are shown. Original magnification, 400. (B) Mean glomerular fluorescence intensity (arbitrary units) was determined for IgG and C3 in TLR9 +/+ ( n = 8) and TLR9 −/− ( n = 8) mice. Horizontal lines represent mean values.
    Biotin Labeled Goat Anti Mouse Igg, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore goat anti human igg
    Glomerular immune deposits do not require anti-DNA antibodies. (A) Glomerular immune deposits were detected by direct immunofluorescence for <t>IgG</t> (top) and complement C3 (bottom) in frozen kidney sections from <t>TLR9</t> +/+ (left), TLR9 −/− (middle), and nonautoimmune C57BL/6 control mice (right). Representative images are shown. Original magnification, 400. (B) Mean glomerular fluorescence intensity (arbitrary units) was determined for IgG and C3 in TLR9 +/+ ( n = 8) and TLR9 −/− ( n = 8) mice. Horizontal lines represent mean values.
    Goat Anti Human Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 837 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore polyclonal anti mouse igg
    Glomerular immune deposits do not require anti-DNA antibodies. (A) Glomerular immune deposits were detected by direct immunofluorescence for <t>IgG</t> (top) and complement C3 (bottom) in frozen kidney sections from <t>TLR9</t> +/+ (left), TLR9 −/− (middle), and nonautoimmune C57BL/6 control mice (right). Representative images are shown. Original magnification, 400. (B) Mean glomerular fluorescence intensity (arbitrary units) was determined for IgG and C3 in TLR9 +/+ ( n = 8) and TLR9 −/− ( n = 8) mice. Horizontal lines represent mean values.
    Polyclonal Anti Mouse Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure fab fragment goat anti mouse igg
    Glomerular immune deposits do not require anti-DNA antibodies. (A) Glomerular immune deposits were detected by direct immunofluorescence for <t>IgG</t> (top) and complement C3 (bottom) in frozen kidney sections from <t>TLR9</t> +/+ (left), TLR9 −/− (middle), and nonautoimmune C57BL/6 control mice (right). Representative images are shown. Original magnification, 400. (B) Mean glomerular fluorescence intensity (arbitrary units) was determined for IgG and C3 in TLR9 +/+ ( n = 8) and TLR9 −/− ( n = 8) mice. Horizontal lines represent mean values.
    Biotin Sp Affinipure Fab Fragment Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 88/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The production of IgG1 and IgG2a by OVA-immunized BALB/c mice injected with OdDHL. Serum from OVA-immunized BALB/c mice, given either an OdDHL or control injection, was collected, and an ELISA was used to quantify OVA-specific IgG1 (A) and IgG2a (B) levels. Data are represented as the median ± 25th and 75th percentiles (boxes). Error bars represent the maximum and minimum values; n = 7 to 10. *, P

    Journal: Infection and Immunity

    Article Title: Modification of In Vivo and In Vitro T- and B-Cell-Mediated Immune Responses by the Pseudomonas aeruginosa Quorum-Sensing Molecule N-(3-Oxododecanoyl)-l-Homoserine Lactone

    doi: 10.1128/IAI.71.8.4421-4431.2003

    Figure Lengend Snippet: The production of IgG1 and IgG2a by OVA-immunized BALB/c mice injected with OdDHL. Serum from OVA-immunized BALB/c mice, given either an OdDHL or control injection, was collected, and an ELISA was used to quantify OVA-specific IgG1 (A) and IgG2a (B) levels. Data are represented as the median ± 25th and 75th percentiles (boxes). Error bars represent the maximum and minimum values; n = 7 to 10. *, P

    Article Snippet: Plates were incubated for 3 h at room temperature and were washed three times, and 100-μl/well concentrations of 1 μg of biotin-labeled rat anti-mouse IgG2a heavy chain-specific antibody (Serotec Ltd., Oxford, United Kingdom)/ml or 250 ng of rat-anti mouse IgG1 heavy chain-specific antibody (Serotec)/ml were added and incubated for 2 h. Plates were washed three times, and 100 μl of a 1:8,000 dilution of streptavidin-horseradish peroxidase per well was added and incubated for 1 h. Plates were washed three times, and 100 μl of ELISA substrate per well was added and allowed to develop in the dark for 1 h before the addition of 100 μl of 0.2 M sulfuric acid/well.

    Techniques: Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay

    Effect of Stat6 deficiency on serum OVA-specific IgG1 ( A ) and OVA-specific IgG2a ( B ) antibody levels of mice 3 d after a single antigen or PBS aspiration challenge. Mice were treated as described in Fig. 1 . Serum was diluted 1:100, 1: 250, and 1:500 with FBS for analysis of OVA-specific IgG1 and IgG2a antibody levels by ELISA. Since recombinant OVA-specific antibodies were not available to generate a standard curve, relative antibody levels are reported using OD readings obtained after background absorbance was subtracted. Reported OD values are from samples diluted 1:250, all of which were below the saturation point of the assay as demonstrated by comparison to OD values obtained from samples diluted 1:100. Values are reported as mean and SE ( n = 9). * P value

    Journal: The Journal of Experimental Medicine

    Article Title: Signal Transducer and Activator of Transcription Factor 6 (Stat6)-deficient Mice Are Protected from Antigen-induced Airway Hyperresponsiveness and Mucus Production

    doi:

    Figure Lengend Snippet: Effect of Stat6 deficiency on serum OVA-specific IgG1 ( A ) and OVA-specific IgG2a ( B ) antibody levels of mice 3 d after a single antigen or PBS aspiration challenge. Mice were treated as described in Fig. 1 . Serum was diluted 1:100, 1: 250, and 1:500 with FBS for analysis of OVA-specific IgG1 and IgG2a antibody levels by ELISA. Since recombinant OVA-specific antibodies were not available to generate a standard curve, relative antibody levels are reported using OD readings obtained after background absorbance was subtracted. Reported OD values are from samples diluted 1:250, all of which were below the saturation point of the assay as demonstrated by comparison to OD values obtained from samples diluted 1:100. Values are reported as mean and SE ( n = 9). * P value

    Article Snippet: Plates were washed with 0.05% Tween-20/PBS and incubated with biotin-conjugated anti– mouse IgG1 (1/2,000; γl chain specific) or IgG2a (1/2,000; γ2a chain specific; PharMingen , San Diego, CA; 100 μl/well) at room temperature for 1 h. After another wash procedure, plates were incubated for 1 h at room temperature with 100 μl/well of 0.0025 mg/ml avidin peroxidase ( Sigma Chemical Co. ) in 10% FBS/PBS.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Recombinant

    Immunohistochemical detection of VCAM-1 expression in lung sections from antigen-treated wild-type and Stat6-deficient mice treated as described in Fig. 1 legend. Lung sections ( n = 2, four sections per animal) from either antigen-treated wild-type ( A and B ) or Stat6-deficient animals ( C and D ) were incubated with either isotype-matched control IgG mAb ( A and C ) or anti–VCAM-1 mAb ( B and D ). Notice comparable VCAM-1–positive staining in sections from antigen-treated wild-type and Stat6-deficient mice ( B and D ). Notice lack of positive staining in sections incubated with the isotype-matched control antibody ( A and C ). Bars, 50 μm.

    Journal: The Journal of Experimental Medicine

    Article Title: Signal Transducer and Activator of Transcription Factor 6 (Stat6)-deficient Mice Are Protected from Antigen-induced Airway Hyperresponsiveness and Mucus Production

    doi:

    Figure Lengend Snippet: Immunohistochemical detection of VCAM-1 expression in lung sections from antigen-treated wild-type and Stat6-deficient mice treated as described in Fig. 1 legend. Lung sections ( n = 2, four sections per animal) from either antigen-treated wild-type ( A and B ) or Stat6-deficient animals ( C and D ) were incubated with either isotype-matched control IgG mAb ( A and C ) or anti–VCAM-1 mAb ( B and D ). Notice comparable VCAM-1–positive staining in sections from antigen-treated wild-type and Stat6-deficient mice ( B and D ). Notice lack of positive staining in sections incubated with the isotype-matched control antibody ( A and C ). Bars, 50 μm.

    Article Snippet: Plates were washed with 0.05% Tween-20/PBS and incubated with biotin-conjugated anti– mouse IgG1 (1/2,000; γl chain specific) or IgG2a (1/2,000; γ2a chain specific; PharMingen , San Diego, CA; 100 μl/well) at room temperature for 1 h. After another wash procedure, plates were incubated for 1 h at room temperature with 100 μl/well of 0.0025 mg/ml avidin peroxidase ( Sigma Chemical Co. ) in 10% FBS/PBS.

    Techniques: Immunohistochemistry, Expressing, Mouse Assay, Incubation, Staining

    Glomerular immune deposits do not require anti-DNA antibodies. (A) Glomerular immune deposits were detected by direct immunofluorescence for IgG (top) and complement C3 (bottom) in frozen kidney sections from TLR9 +/+ (left), TLR9 −/− (middle), and nonautoimmune C57BL/6 control mice (right). Representative images are shown. Original magnification, 400. (B) Mean glomerular fluorescence intensity (arbitrary units) was determined for IgG and C3 in TLR9 +/+ ( n = 8) and TLR9 −/− ( n = 8) mice. Horizontal lines represent mean values.

    Journal: The Journal of Experimental Medicine

    Article Title: Toll-like receptor 9 controls anti-DNA autoantibody production in murine lupus

    doi: 10.1084/jem.20050338

    Figure Lengend Snippet: Glomerular immune deposits do not require anti-DNA antibodies. (A) Glomerular immune deposits were detected by direct immunofluorescence for IgG (top) and complement C3 (bottom) in frozen kidney sections from TLR9 +/+ (left), TLR9 −/− (middle), and nonautoimmune C57BL/6 control mice (right). Representative images are shown. Original magnification, 400. (B) Mean glomerular fluorescence intensity (arbitrary units) was determined for IgG and C3 in TLR9 +/+ ( n = 8) and TLR9 −/− ( n = 8) mice. Horizontal lines represent mean values.

    Article Snippet: For IgG deposition, frozen kidneys from eight TLR9+/+ and eight TLR9−/− mice were sectioned and stained with biotin-conjugated goat anti–mouse IgG (γ chain specific; Southern Biotechnology Associates, Inc.) and Alexa Fluor 647–conjugated streptavidin (Molecular Probes).

    Techniques: Immunofluorescence, Mouse Assay, Fluorescence

    Lymphadenopathy, hypergammaglobulinemia, and lymphocyte accumulation in TLR-deficient mice. TLR9 +/+ ( n = 19), TLR9 −/− ( n = 16), TLR3 +/+ ( n = 15), and TLR3 −/− ( n = 17) mice were killed at 20 wk of age and assessed for evidence of aberrant immune activation; nonautoimmune C57BL/6 control mice ( n = 4) were killed at 26 wk of age. (A) Spleens and the two largest axillary lymph nodes were removed and weighed. (B) Total serum IgG and IgG2a were determined. (C) Splenocyte subsets were enumerated by FACS analysis for T cells (Thy1.2 + ), DNTC (CD4 − /CD8 − double-negative T cells), and B cells (CD22 + ). (D) Splenic CD4 + T cells were classified as either naive (CD44 − CD62L + ), activated (CD44 + CD62L + ), or memory (CD44 + CD62L − ) phenotype. The analysis in D was performed on 12 TLR3 +/+ and 12 TLR3 −/− mice. Horizontal lines represent mean values.

    Journal: The Journal of Experimental Medicine

    Article Title: Toll-like receptor 9 controls anti-DNA autoantibody production in murine lupus

    doi: 10.1084/jem.20050338

    Figure Lengend Snippet: Lymphadenopathy, hypergammaglobulinemia, and lymphocyte accumulation in TLR-deficient mice. TLR9 +/+ ( n = 19), TLR9 −/− ( n = 16), TLR3 +/+ ( n = 15), and TLR3 −/− ( n = 17) mice were killed at 20 wk of age and assessed for evidence of aberrant immune activation; nonautoimmune C57BL/6 control mice ( n = 4) were killed at 26 wk of age. (A) Spleens and the two largest axillary lymph nodes were removed and weighed. (B) Total serum IgG and IgG2a were determined. (C) Splenocyte subsets were enumerated by FACS analysis for T cells (Thy1.2 + ), DNTC (CD4 − /CD8 − double-negative T cells), and B cells (CD22 + ). (D) Splenic CD4 + T cells were classified as either naive (CD44 − CD62L + ), activated (CD44 + CD62L + ), or memory (CD44 + CD62L − ) phenotype. The analysis in D was performed on 12 TLR3 +/+ and 12 TLR3 −/− mice. Horizontal lines represent mean values.

    Article Snippet: For IgG deposition, frozen kidneys from eight TLR9+/+ and eight TLR9−/− mice were sectioned and stained with biotin-conjugated goat anti–mouse IgG (γ chain specific; Southern Biotechnology Associates, Inc.) and Alexa Fluor 647–conjugated streptavidin (Molecular Probes).

    Techniques: Mouse Assay, Activation Assay, FACS

    Reduced anti-dsDNA autoantibodies in TLR9 − / − but not TLR3 − / − mice. (A) Anti-dsDNA antibodies were detected by C. luciliae immunofluorescence. IgG antibodies to C. luciliae DNA are shown in green (left), and DAPI staining of DNA is shown in red (middle). White arrows indicate the kinetoplast. Specific anti-dsDNA antibodies are identified by colocalization of IgG and DAPI staining in the kinetoplast and appear in yellow (right). Representative TLR9 +/+ sera are shown in the top two rows (intensity scores of 3 + and 1 + ), and TLR9 −/− sera are shown in the bottom two rows (intensity scores of 1 + and 0). Original magnification, 1,000. (B and C) Specific anti-dsDNA staining of C. luciliae kinetoplasts was scored from 0 to 4 as in A for either TLR9 +/+ ( n = 19) and TLR9 −/− ( n = 16) sera (B), or TLR3 +/+ ( n = 15) and TLR3 −/− ( n = 17) sera (C). *, P

    Journal: The Journal of Experimental Medicine

    Article Title: Toll-like receptor 9 controls anti-DNA autoantibody production in murine lupus

    doi: 10.1084/jem.20050338

    Figure Lengend Snippet: Reduced anti-dsDNA autoantibodies in TLR9 − / − but not TLR3 − / − mice. (A) Anti-dsDNA antibodies were detected by C. luciliae immunofluorescence. IgG antibodies to C. luciliae DNA are shown in green (left), and DAPI staining of DNA is shown in red (middle). White arrows indicate the kinetoplast. Specific anti-dsDNA antibodies are identified by colocalization of IgG and DAPI staining in the kinetoplast and appear in yellow (right). Representative TLR9 +/+ sera are shown in the top two rows (intensity scores of 3 + and 1 + ), and TLR9 −/− sera are shown in the bottom two rows (intensity scores of 1 + and 0). Original magnification, 1,000. (B and C) Specific anti-dsDNA staining of C. luciliae kinetoplasts was scored from 0 to 4 as in A for either TLR9 +/+ ( n = 19) and TLR9 −/− ( n = 16) sera (B), or TLR3 +/+ ( n = 15) and TLR3 −/− ( n = 17) sera (C). *, P

    Article Snippet: For IgG deposition, frozen kidneys from eight TLR9+/+ and eight TLR9−/− mice were sectioned and stained with biotin-conjugated goat anti–mouse IgG (γ chain specific; Southern Biotechnology Associates, Inc.) and Alexa Fluor 647–conjugated streptavidin (Molecular Probes).

    Techniques: Mouse Assay, Immunofluorescence, Staining