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    Santa Cruz Biotechnology mouse factor h
    Mouse Factor H, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse factor h/product/Santa Cruz Biotechnology
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    86
    Quidel mouse factor h
    Mouse Factor H, supplied by Quidel, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse factor h/product/Quidel
    Average 86 stars, based on 1 article reviews
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    86
    Bio-Rad mouse anti human factor h
    C3 fragments present on the surface of S. aureus Reynolds after incubation with antibody and purified complement factors C1, C4, C2, and C3 (C1423). Some fractions were then incubated with factor H, factor I, or both (H&I). Surface-bound C3 fragments were released by incubation with methylamine (A) or allowed to spontaneously shed from the S. aureus surface (B). Western blot analysis with polyclonal anti-C3 antibody showed that incubation in factor I or in factors H and I together was associated with bound and shed C3b (104 and 75 kDa) and iC3b (75, 63, and 42 kDa). Purified C3b and iC3b are shown for comparison.
    Mouse Anti Human Factor H, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human factor h/product/Bio-Rad
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti human factor h - by Bioz Stars, 2023-11
    86/100 stars
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    86
    Abcam monoclonal mouse anti factor h
    Glomerular FHR5 staining in C3 glomerulopathy. (a) Glomerular staining intensity in native and transplant biopsies. FHR5 was the most frequent protein at 3+ intensity and detected in all of the transplant biopsies. (b) Representative images of complement staining in the native kidney. For each set of images, the biopsy indication, the appearances of the glomeruli by light microscopy, and the estimated glomerular filtration rate (eGFR) and proteinuria at the time of biopsy are indicated. *The cases represented by the fourth and fifth rows of images are from the same family. (c) Representative images of complement staining in transplant kidneys. For each set of images, the biopsy indication (either protocol or due to clinical changes; i.e., indication biopsy), time posttransplant together with the eGFR, and proteinuria at the time of biopsy are indicated. The histology including the presence or absence of rejection and the histology of subsequent biopsies is also shown. C3G, C3 glomerulopathy; C3GN, C3 glomerulonephritis; EH, endocapillary hypercellularity; fH, factor H; FHR1, factor H–related protein 1; FHR5, factor H–related protein 5; FHR5 mutation, the mutation described in CFHR5 nephropathy  ; MPGN, membranoproliferative glomerulonephritis; NS, nephrotic syndrome; UPCR, urinary protein:creatinine ratio. Original magnification ×400. Bars = 100 μm.
    Monoclonal Mouse Anti Factor H, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal mouse anti factor h/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal mouse anti factor h - by Bioz Stars, 2023-11
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    80
    Santa Cruz Biotechnology mouse anti human factor h antibody
    Glomerular FHR5 staining in C3 glomerulopathy. (a) Glomerular staining intensity in native and transplant biopsies. FHR5 was the most frequent protein at 3+ intensity and detected in all of the transplant biopsies. (b) Representative images of complement staining in the native kidney. For each set of images, the biopsy indication, the appearances of the glomeruli by light microscopy, and the estimated glomerular filtration rate (eGFR) and proteinuria at the time of biopsy are indicated. *The cases represented by the fourth and fifth rows of images are from the same family. (c) Representative images of complement staining in transplant kidneys. For each set of images, the biopsy indication (either protocol or due to clinical changes; i.e., indication biopsy), time posttransplant together with the eGFR, and proteinuria at the time of biopsy are indicated. The histology including the presence or absence of rejection and the histology of subsequent biopsies is also shown. C3G, C3 glomerulopathy; C3GN, C3 glomerulonephritis; EH, endocapillary hypercellularity; fH, factor H; FHR1, factor H–related protein 1; FHR5, factor H–related protein 5; FHR5 mutation, the mutation described in CFHR5 nephropathy  ; MPGN, membranoproliferative glomerulonephritis; NS, nephrotic syndrome; UPCR, urinary protein:creatinine ratio. Original magnification ×400. Bars = 100 μm.
    Mouse Anti Human Factor H Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human factor h antibody/product/Santa Cruz Biotechnology
    Average 80 stars, based on 1 article reviews
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    mouse anti human factor h antibody - by Bioz Stars, 2023-11
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    Image Search Results


    C3 fragments present on the surface of S. aureus Reynolds after incubation with antibody and purified complement factors C1, C4, C2, and C3 (C1423). Some fractions were then incubated with factor H, factor I, or both (H&I). Surface-bound C3 fragments were released by incubation with methylamine (A) or allowed to spontaneously shed from the S. aureus surface (B). Western blot analysis with polyclonal anti-C3 antibody showed that incubation in factor I or in factors H and I together was associated with bound and shed C3b (104 and 75 kDa) and iC3b (75, 63, and 42 kDa). Purified C3b and iC3b are shown for comparison.

    Journal:

    Article Title: Cleavage of Complement C3b to iC3b on the Surface of Staphylococcus aureus Is Mediated by Serum Complement Factor I

    doi: 10.1128/IAI.72.5.2858-2863.2004

    Figure Lengend Snippet: C3 fragments present on the surface of S. aureus Reynolds after incubation with antibody and purified complement factors C1, C4, C2, and C3 (C1423). Some fractions were then incubated with factor H, factor I, or both (H&I). Surface-bound C3 fragments were released by incubation with methylamine (A) or allowed to spontaneously shed from the S. aureus surface (B). Western blot analysis with polyclonal anti-C3 antibody showed that incubation in factor I or in factors H and I together was associated with bound and shed C3b (104 and 75 kDa) and iC3b (75, 63, and 42 kDa). Purified C3b and iC3b are shown for comparison.

    Article Snippet: Flat-bottom Immulon 2 plates were coated with mouse anti-human factor H (Serotec Ltd., Oxford, United Kingdom) at 20 μg/ml in a carbonate coating buffer overnight at 4°C.

    Techniques: Incubation, Purification, Western Blot

    C3 fragments shed from the surface of S. aureus Reynolds after incubation with antibody and purified complement factors C1, C4, C2, and C3 (C1243 in panel A, C1423 in panel B). Some fractions were then incubated with factor H, factor I, or both (H&I). C3 fragments were allowed to spontaneously shed from the S. aureus surface. (A) Total protein staining of the SDS-PAGE gel showed that incubation in factor I or in factors H and I together was associated with decreased C3b (104 and 75 kDa) and increased iC3b (75, 63, and 42 kDa). (B) Optical densitometry measurements of C3b (104-kDa band) and iC3b (42-kDa band) were calculated as a ratio of the 75-kDa band. Less C3b and more iC3b were present after incubation in factor I (P = 0.002 and P = 0.002, respectively) or in factors H and I together (P = 0.022 and P = 0.047, respectively) than after incubation in buffer alone. Data are means of results from three independent experiments. Error bars denote standard errors of the means.

    Journal:

    Article Title: Cleavage of Complement C3b to iC3b on the Surface of Staphylococcus aureus Is Mediated by Serum Complement Factor I

    doi: 10.1128/IAI.72.5.2858-2863.2004

    Figure Lengend Snippet: C3 fragments shed from the surface of S. aureus Reynolds after incubation with antibody and purified complement factors C1, C4, C2, and C3 (C1243 in panel A, C1423 in panel B). Some fractions were then incubated with factor H, factor I, or both (H&I). C3 fragments were allowed to spontaneously shed from the S. aureus surface. (A) Total protein staining of the SDS-PAGE gel showed that incubation in factor I or in factors H and I together was associated with decreased C3b (104 and 75 kDa) and increased iC3b (75, 63, and 42 kDa). (B) Optical densitometry measurements of C3b (104-kDa band) and iC3b (42-kDa band) were calculated as a ratio of the 75-kDa band. Less C3b and more iC3b were present after incubation in factor I (P = 0.002 and P = 0.002, respectively) or in factors H and I together (P = 0.022 and P = 0.047, respectively) than after incubation in buffer alone. Data are means of results from three independent experiments. Error bars denote standard errors of the means.

    Article Snippet: Flat-bottom Immulon 2 plates were coated with mouse anti-human factor H (Serotec Ltd., Oxford, United Kingdom) at 20 μg/ml in a carbonate coating buffer overnight at 4°C.

    Techniques: Incubation, Purification, Staining, SDS Page

    Various concentrations of factor H bound to mid-logarithmic-phase S. aureus Reynolds. Western blot analysis with anti-factor H antibody (A) and factor H ELISA (B) showed that factor H binding to S. aureus Reynolds increased with incubation in higher concentrations of factor H in a dose-response fashion. S. aureus Reynolds was incubated in different quantities of factor H in EDTA-GVBS−− buffer for 60 min at 37°C. Data are means of results from at least three independent experiments. Error bars denote standard errors of the means.

    Journal:

    Article Title: Cleavage of Complement C3b to iC3b on the Surface of Staphylococcus aureus Is Mediated by Serum Complement Factor I

    doi: 10.1128/IAI.72.5.2858-2863.2004

    Figure Lengend Snippet: Various concentrations of factor H bound to mid-logarithmic-phase S. aureus Reynolds. Western blot analysis with anti-factor H antibody (A) and factor H ELISA (B) showed that factor H binding to S. aureus Reynolds increased with incubation in higher concentrations of factor H in a dose-response fashion. S. aureus Reynolds was incubated in different quantities of factor H in EDTA-GVBS−− buffer for 60 min at 37°C. Data are means of results from at least three independent experiments. Error bars denote standard errors of the means.

    Article Snippet: Flat-bottom Immulon 2 plates were coated with mouse anti-human factor H (Serotec Ltd., Oxford, United Kingdom) at 20 μg/ml in a carbonate coating buffer overnight at 4°C.

    Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Binding Assay, Incubation

    Purified factor H bound to mid-logarithmic-phase S. aureus Reynolds incubated in various concentrations of BSA. Factor H ELISA showed that factor H binding to S. aureus decreased in the presence of excess BSA. S. aureus Reynolds was incubated in factor H and BSA for 60 min at 37°C. Each datum point represents the mean of results from three independent experiments. Error bars denote standard errors of the means.

    Journal:

    Article Title: Cleavage of Complement C3b to iC3b on the Surface of Staphylococcus aureus Is Mediated by Serum Complement Factor I

    doi: 10.1128/IAI.72.5.2858-2863.2004

    Figure Lengend Snippet: Purified factor H bound to mid-logarithmic-phase S. aureus Reynolds incubated in various concentrations of BSA. Factor H ELISA showed that factor H binding to S. aureus decreased in the presence of excess BSA. S. aureus Reynolds was incubated in factor H and BSA for 60 min at 37°C. Each datum point represents the mean of results from three independent experiments. Error bars denote standard errors of the means.

    Article Snippet: Flat-bottom Immulon 2 plates were coated with mouse anti-human factor H (Serotec Ltd., Oxford, United Kingdom) at 20 μg/ml in a carbonate coating buffer overnight at 4°C.

    Techniques: Purification, Incubation, Enzyme-linked Immunosorbent Assay, Binding Assay

    Glomerular FHR5 staining in C3 glomerulopathy. (a) Glomerular staining intensity in native and transplant biopsies. FHR5 was the most frequent protein at 3+ intensity and detected in all of the transplant biopsies. (b) Representative images of complement staining in the native kidney. For each set of images, the biopsy indication, the appearances of the glomeruli by light microscopy, and the estimated glomerular filtration rate (eGFR) and proteinuria at the time of biopsy are indicated. *The cases represented by the fourth and fifth rows of images are from the same family. (c) Representative images of complement staining in transplant kidneys. For each set of images, the biopsy indication (either protocol or due to clinical changes; i.e., indication biopsy), time posttransplant together with the eGFR, and proteinuria at the time of biopsy are indicated. The histology including the presence or absence of rejection and the histology of subsequent biopsies is also shown. C3G, C3 glomerulopathy; C3GN, C3 glomerulonephritis; EH, endocapillary hypercellularity; fH, factor H; FHR1, factor H–related protein 1; FHR5, factor H–related protein 5; FHR5 mutation, the mutation described in CFHR5 nephropathy  ; MPGN, membranoproliferative glomerulonephritis; NS, nephrotic syndrome; UPCR, urinary protein:creatinine ratio. Original magnification ×400. Bars = 100 μm.

    Journal: Kidney International Reports

    Article Title: Glomerular Complement Factor H–Related Protein 5 (FHR5) Is Highly Prevalent in C3 Glomerulopathy and Associated With Renal Impairment

    doi: 10.1016/j.ekir.2019.06.008

    Figure Lengend Snippet: Glomerular FHR5 staining in C3 glomerulopathy. (a) Glomerular staining intensity in native and transplant biopsies. FHR5 was the most frequent protein at 3+ intensity and detected in all of the transplant biopsies. (b) Representative images of complement staining in the native kidney. For each set of images, the biopsy indication, the appearances of the glomeruli by light microscopy, and the estimated glomerular filtration rate (eGFR) and proteinuria at the time of biopsy are indicated. *The cases represented by the fourth and fifth rows of images are from the same family. (c) Representative images of complement staining in transplant kidneys. For each set of images, the biopsy indication (either protocol or due to clinical changes; i.e., indication biopsy), time posttransplant together with the eGFR, and proteinuria at the time of biopsy are indicated. The histology including the presence or absence of rejection and the histology of subsequent biopsies is also shown. C3G, C3 glomerulopathy; C3GN, C3 glomerulonephritis; EH, endocapillary hypercellularity; fH, factor H; FHR1, factor H–related protein 1; FHR5, factor H–related protein 5; FHR5 mutation, the mutation described in CFHR5 nephropathy ; MPGN, membranoproliferative glomerulonephritis; NS, nephrotic syndrome; UPCR, urinary protein:creatinine ratio. Original magnification ×400. Bars = 100 μm.

    Article Snippet: Primary antibodies were polyclonal rabbit anti-C3c (#A0062; Dako, Glostrup, Denmark); polyclonal rabbit anti-C3d (#136916; Abcam, Cambridge, UK) that recognizes C3dg; polyclonal rabbit anti-properdin (#2097; Biorbyt, Cambridge, UK); monoclonal mouse anti-C5b9 (#M0777; Dako); polyclonal rabbit anti-C4d (#107-01; BD Biotech, Franklin Lakes, NJ); monoclonal rabbit anti-C1q (#A0136; Dako); monoclonal mouse anti-factor H, OX-24 (#118820; Abcam); monoclonal mouse anti-FHR1 (#3078-M01; Abnova, Taipei, Taiwan); polyclonal rabbit anti-FHR5 (#81494-D01P; Abnova); and monoclonal mouse anti-CD68 (#M0876; Dako).

    Techniques: Staining, Light Microscopy, Filtration, Mutagenesis

    Sequential glomerular FHR5 staining in a single case of C3 glomerulopathy transplant recurrence. Images and information from the same biopsy are organized in columns. A protocol surveillance biopsy performed 3 months after transplantation (Transplant biopsy 1) showed recurrent C3 glomerulonephritis (C3GN) with mild endocapillary hypercellularity (EH). Glomerular factor H–related protein 5 (FHR5), C5b9, C3b/iC3b/C3c, and C3dg were detected. The estimated glomerular filtration rate (eGFR) was stable and there was no significant proteinuria. Approximately 6 months posttransplant, the patient developed proteinuria and a fall in eGFR. Biopsy showed crescentic C3GN and increased glomerular CD68-positive cells (Transplant biopsy 2). Glomerular FHR5, C5b9, C3b/iC3b/C3c, and C3dg were detected but at increased staining intensity compared with the first transplant biopsy. The eGFR and proteinuria improved with eculizumab and prednisolone treatment. After 4 months of eculizumab treatment, renal biopsy (Transplant biopsy 3) showed resolution of glomerular CD68 staining, but glomerular staining for FHR5, C5b9, C3b/iC3b/C3c, and C3dg remained unchanged. After a further 3 months, proteinuria increased and biopsy showed crescentic C3GN and the recurrence of glomerular CD68-positive cells (Transplant biopsy 4). Proteinuria improved with re-introduction of eculizumab. UPCR, urine protein:creatinine ratio. Original magnification ×200 or ×400. Bars = 100 μm.

    Journal: Kidney International Reports

    Article Title: Glomerular Complement Factor H–Related Protein 5 (FHR5) Is Highly Prevalent in C3 Glomerulopathy and Associated With Renal Impairment

    doi: 10.1016/j.ekir.2019.06.008

    Figure Lengend Snippet: Sequential glomerular FHR5 staining in a single case of C3 glomerulopathy transplant recurrence. Images and information from the same biopsy are organized in columns. A protocol surveillance biopsy performed 3 months after transplantation (Transplant biopsy 1) showed recurrent C3 glomerulonephritis (C3GN) with mild endocapillary hypercellularity (EH). Glomerular factor H–related protein 5 (FHR5), C5b9, C3b/iC3b/C3c, and C3dg were detected. The estimated glomerular filtration rate (eGFR) was stable and there was no significant proteinuria. Approximately 6 months posttransplant, the patient developed proteinuria and a fall in eGFR. Biopsy showed crescentic C3GN and increased glomerular CD68-positive cells (Transplant biopsy 2). Glomerular FHR5, C5b9, C3b/iC3b/C3c, and C3dg were detected but at increased staining intensity compared with the first transplant biopsy. The eGFR and proteinuria improved with eculizumab and prednisolone treatment. After 4 months of eculizumab treatment, renal biopsy (Transplant biopsy 3) showed resolution of glomerular CD68 staining, but glomerular staining for FHR5, C5b9, C3b/iC3b/C3c, and C3dg remained unchanged. After a further 3 months, proteinuria increased and biopsy showed crescentic C3GN and the recurrence of glomerular CD68-positive cells (Transplant biopsy 4). Proteinuria improved with re-introduction of eculizumab. UPCR, urine protein:creatinine ratio. Original magnification ×200 or ×400. Bars = 100 μm.

    Article Snippet: Primary antibodies were polyclonal rabbit anti-C3c (#A0062; Dako, Glostrup, Denmark); polyclonal rabbit anti-C3d (#136916; Abcam, Cambridge, UK) that recognizes C3dg; polyclonal rabbit anti-properdin (#2097; Biorbyt, Cambridge, UK); monoclonal mouse anti-C5b9 (#M0777; Dako); polyclonal rabbit anti-C4d (#107-01; BD Biotech, Franklin Lakes, NJ); monoclonal rabbit anti-C1q (#A0136; Dako); monoclonal mouse anti-factor H, OX-24 (#118820; Abcam); monoclonal mouse anti-FHR1 (#3078-M01; Abnova, Taipei, Taiwan); polyclonal rabbit anti-FHR5 (#81494-D01P; Abnova); and monoclonal mouse anti-CD68 (#M0876; Dako).

    Techniques: Staining, Transplantation Assay, Filtration

    Glomerular factor H–related protein 5 (FHR5) colocalizes with C3 in C3 glomerulopathy. (a) Glomerular FHR5 staining intensity positively correlated with the staining intensity of C3b/iC3b/C3c, C3dg, and C5b9. Both glomerular C3b/iC3b/C3c and C3dg positively correlated with glomerular C5b9. R values are calculated from Pearson’s correlation coefficient and P values are adjusted for multiple comparisons. (b) Representative images of combined immunofluorescence staining in three C3G cases for glomerular FHR5 with either C3b/iC3b/C3c or C3dg, and C3b/iC3b/C3c with C3dg. Renal biopsies in all 3 cases showed C3-dominant membranoproliferative glomerulonephritis, and the biopsy indications together with the urine protein:creatinine ratio (UPCR), estimated glomerular filtration rate (eGFR), and serum C3 levels at the time of biopsy are listed. The staining patterns for C3b/iC3b/C3c and C3dg (right-hand column of images) showed areas of colocalization (arrows), areas of C3b/iC3b/C3c alone (stars), and areas of C3dg alone (triangles). The staining pattern for C3b/iC3b/C3c and FHR5 (left-hand column of images) showed areas of colocalization (arrows), areas of C3b/iC3b/C3c alone (stars), and areas of FHR5 alone (triangles). The staining pattern for C3dg and FHR5 (middle column of images) showed areas of colocalization, particularly along capillary walls in cases 2 and 3 (arrows) and areas of FHR5 alone in cases 1 and 2 (triangles). Notably, we did not detect areas of C3dg without FHR5 staining. (c) FHR5, C3b/iC3b/C3c, and C3dg glomerular locations correlate in C3G. We calculated the correlation of glomerular antigen locations in all available glomeruli from the three C3G cases (c). The Pearson’s correlation coefficient for all glomeruli from cases 1 (circles), 2 (squares), and 3 (triangles), and the median values of the correlations for each case (horizontal lines) are shown. The median values of the correlation coefficients ( r ) for each case were as follows: C3b/iC3b/C3c with FHR5: 0.73 (case 1), 0.76 (case 2), and 0.68 (case 3); FHR5 with C3dg: 0.71 (case 1), 0.90 (case 2), and 0.5 (case 3); and C3b/iC3b/C3c with FHR5: 0.68 (case 1), 0.81 (case 2), and 0.58 (case 3). MMF, mycophenolate mofetil. Original magnification ×400. Bars = 100 μm.

    Journal: Kidney International Reports

    Article Title: Glomerular Complement Factor H–Related Protein 5 (FHR5) Is Highly Prevalent in C3 Glomerulopathy and Associated With Renal Impairment

    doi: 10.1016/j.ekir.2019.06.008

    Figure Lengend Snippet: Glomerular factor H–related protein 5 (FHR5) colocalizes with C3 in C3 glomerulopathy. (a) Glomerular FHR5 staining intensity positively correlated with the staining intensity of C3b/iC3b/C3c, C3dg, and C5b9. Both glomerular C3b/iC3b/C3c and C3dg positively correlated with glomerular C5b9. R values are calculated from Pearson’s correlation coefficient and P values are adjusted for multiple comparisons. (b) Representative images of combined immunofluorescence staining in three C3G cases for glomerular FHR5 with either C3b/iC3b/C3c or C3dg, and C3b/iC3b/C3c with C3dg. Renal biopsies in all 3 cases showed C3-dominant membranoproliferative glomerulonephritis, and the biopsy indications together with the urine protein:creatinine ratio (UPCR), estimated glomerular filtration rate (eGFR), and serum C3 levels at the time of biopsy are listed. The staining patterns for C3b/iC3b/C3c and C3dg (right-hand column of images) showed areas of colocalization (arrows), areas of C3b/iC3b/C3c alone (stars), and areas of C3dg alone (triangles). The staining pattern for C3b/iC3b/C3c and FHR5 (left-hand column of images) showed areas of colocalization (arrows), areas of C3b/iC3b/C3c alone (stars), and areas of FHR5 alone (triangles). The staining pattern for C3dg and FHR5 (middle column of images) showed areas of colocalization, particularly along capillary walls in cases 2 and 3 (arrows) and areas of FHR5 alone in cases 1 and 2 (triangles). Notably, we did not detect areas of C3dg without FHR5 staining. (c) FHR5, C3b/iC3b/C3c, and C3dg glomerular locations correlate in C3G. We calculated the correlation of glomerular antigen locations in all available glomeruli from the three C3G cases (c). The Pearson’s correlation coefficient for all glomeruli from cases 1 (circles), 2 (squares), and 3 (triangles), and the median values of the correlations for each case (horizontal lines) are shown. The median values of the correlation coefficients ( r ) for each case were as follows: C3b/iC3b/C3c with FHR5: 0.73 (case 1), 0.76 (case 2), and 0.68 (case 3); FHR5 with C3dg: 0.71 (case 1), 0.90 (case 2), and 0.5 (case 3); and C3b/iC3b/C3c with FHR5: 0.68 (case 1), 0.81 (case 2), and 0.58 (case 3). MMF, mycophenolate mofetil. Original magnification ×400. Bars = 100 μm.

    Article Snippet: Primary antibodies were polyclonal rabbit anti-C3c (#A0062; Dako, Glostrup, Denmark); polyclonal rabbit anti-C3d (#136916; Abcam, Cambridge, UK) that recognizes C3dg; polyclonal rabbit anti-properdin (#2097; Biorbyt, Cambridge, UK); monoclonal mouse anti-C5b9 (#M0777; Dako); polyclonal rabbit anti-C4d (#107-01; BD Biotech, Franklin Lakes, NJ); monoclonal rabbit anti-C1q (#A0136; Dako); monoclonal mouse anti-factor H, OX-24 (#118820; Abcam); monoclonal mouse anti-FHR1 (#3078-M01; Abnova, Taipei, Taiwan); polyclonal rabbit anti-FHR5 (#81494-D01P; Abnova); and monoclonal mouse anti-CD68 (#M0876; Dako).

    Techniques: Staining, Immunofluorescence, Filtration

    Glomerular factor H–related protein 1 (FHR5) and C5b9 staining intensity associated with lower estimated glomerular filtration rate (eGFR) at biopsy in C3 glomerulopathy. The eGFR at the time of biopsy was significantly lower in biopsies that had maximal staining intensities for FHR5 ( P = 0.04, difference of medians 19.7 ml/min per 1.73 m 2 ; 95% confidence interval [CI] 1.1–43.0) and C5b-9 ( P = 0.03, difference of medians 14.86 ml/min per 1.73 m 2 ; 95% CI 3.8–46.6). This was not seen for glomerular factor H–related protein 1 (FHR1), C3b/iC3b/C3c, C3dg, and C4d. P values are derived from Mann-Whitney U tests.

    Journal: Kidney International Reports

    Article Title: Glomerular Complement Factor H–Related Protein 5 (FHR5) Is Highly Prevalent in C3 Glomerulopathy and Associated With Renal Impairment

    doi: 10.1016/j.ekir.2019.06.008

    Figure Lengend Snippet: Glomerular factor H–related protein 1 (FHR5) and C5b9 staining intensity associated with lower estimated glomerular filtration rate (eGFR) at biopsy in C3 glomerulopathy. The eGFR at the time of biopsy was significantly lower in biopsies that had maximal staining intensities for FHR5 ( P = 0.04, difference of medians 19.7 ml/min per 1.73 m 2 ; 95% confidence interval [CI] 1.1–43.0) and C5b-9 ( P = 0.03, difference of medians 14.86 ml/min per 1.73 m 2 ; 95% CI 3.8–46.6). This was not seen for glomerular factor H–related protein 1 (FHR1), C3b/iC3b/C3c, C3dg, and C4d. P values are derived from Mann-Whitney U tests.

    Article Snippet: Primary antibodies were polyclonal rabbit anti-C3c (#A0062; Dako, Glostrup, Denmark); polyclonal rabbit anti-C3d (#136916; Abcam, Cambridge, UK) that recognizes C3dg; polyclonal rabbit anti-properdin (#2097; Biorbyt, Cambridge, UK); monoclonal mouse anti-C5b9 (#M0777; Dako); polyclonal rabbit anti-C4d (#107-01; BD Biotech, Franklin Lakes, NJ); monoclonal rabbit anti-C1q (#A0136; Dako); monoclonal mouse anti-factor H, OX-24 (#118820; Abcam); monoclonal mouse anti-FHR1 (#3078-M01; Abnova, Taipei, Taiwan); polyclonal rabbit anti-FHR5 (#81494-D01P; Abnova); and monoclonal mouse anti-CD68 (#M0876; Dako).

    Techniques: Staining, Filtration, Derivative Assay, MANN-WHITNEY

    Tubulo-interstitial staining for FHR5 in C3 glomerulopathy. (a) Representative images of staining for factor H–related protein 5 (FHR5), factor H–related protein 1 (FHR1), factor H (fH), properdin, and C3b/iC3b/C3c. No tubulo-interstitial staining for FHR5 or fH was evident but there was strong tubulo-interstitial staining for both properdin and FHR1. (b) Tubulo-interstitial staining for properdin was also seen in other renal diseases (thin basement membrane disease [TBM], lupus nephritis [LN], and membranous nephropathy). Tubulo-interstitial staining for FHR1 was demonstrable in biopsies from patients with TBM and IgA nephropathy. Tubulo-interstitial FHR1 staining in TBM was still detectable but less intense when the proteinase XXIV enzyme was used instead of pressure cooker antigen retrieval. Glomerular and tubulo-interstitial FHR1 was absent in renal tissue from a patient with IgA nephropathy and FHR1 deficiency. C3G, C3 glomerulopathy; LN IV(A/C), lupus nephritis class 4, active and chronic. Original magnification ×200. Bars = 100 μm.

    Journal: Kidney International Reports

    Article Title: Glomerular Complement Factor H–Related Protein 5 (FHR5) Is Highly Prevalent in C3 Glomerulopathy and Associated With Renal Impairment

    doi: 10.1016/j.ekir.2019.06.008

    Figure Lengend Snippet: Tubulo-interstitial staining for FHR5 in C3 glomerulopathy. (a) Representative images of staining for factor H–related protein 5 (FHR5), factor H–related protein 1 (FHR1), factor H (fH), properdin, and C3b/iC3b/C3c. No tubulo-interstitial staining for FHR5 or fH was evident but there was strong tubulo-interstitial staining for both properdin and FHR1. (b) Tubulo-interstitial staining for properdin was also seen in other renal diseases (thin basement membrane disease [TBM], lupus nephritis [LN], and membranous nephropathy). Tubulo-interstitial staining for FHR1 was demonstrable in biopsies from patients with TBM and IgA nephropathy. Tubulo-interstitial FHR1 staining in TBM was still detectable but less intense when the proteinase XXIV enzyme was used instead of pressure cooker antigen retrieval. Glomerular and tubulo-interstitial FHR1 was absent in renal tissue from a patient with IgA nephropathy and FHR1 deficiency. C3G, C3 glomerulopathy; LN IV(A/C), lupus nephritis class 4, active and chronic. Original magnification ×200. Bars = 100 μm.

    Article Snippet: Primary antibodies were polyclonal rabbit anti-C3c (#A0062; Dako, Glostrup, Denmark); polyclonal rabbit anti-C3d (#136916; Abcam, Cambridge, UK) that recognizes C3dg; polyclonal rabbit anti-properdin (#2097; Biorbyt, Cambridge, UK); monoclonal mouse anti-C5b9 (#M0777; Dako); polyclonal rabbit anti-C4d (#107-01; BD Biotech, Franklin Lakes, NJ); monoclonal rabbit anti-C1q (#A0136; Dako); monoclonal mouse anti-factor H, OX-24 (#118820; Abcam); monoclonal mouse anti-FHR1 (#3078-M01; Abnova, Taipei, Taiwan); polyclonal rabbit anti-FHR5 (#81494-D01P; Abnova); and monoclonal mouse anti-CD68 (#M0876; Dako).

    Techniques: Staining