anti mhc class ii Search Results


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  • 97
    BioLegend mhc ii
    Human and feline ASCs possess typical MSC characteristics. Feline ( a ) and human ( b ) ASCs adhere to plastic and have a spindle, fibroblast morphology in culture. However, human ASCs are significantly larger than feline ASCs when adherent ( c ) and in suspension ( d ). Feline ( e ) and human ( f ) ASCs both have positive surface expression of CD44, CD90, and CD105, and are negative for surface expression of CD45 (pan leukocyte, human) or <t>CD18</t> (pan leukocyte, feline) and <t>MHC</t> II. Both feline ( g ) and human ( h ) ASCs undergo osteogenic differentiation. Cell size presented as mean and standard error. * P
    Mhc Ii, supplied by BioLegend, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher anti mhc class ii
    Human and feline ASCs possess typical MSC characteristics. Feline ( a ) and human ( b ) ASCs adhere to plastic and have a spindle, fibroblast morphology in culture. However, human ASCs are significantly larger than feline ASCs when adherent ( c ) and in suspension ( d ). Feline ( e ) and human ( f ) ASCs both have positive surface expression of CD44, CD90, and CD105, and are negative for surface expression of CD45 (pan leukocyte, human) or <t>CD18</t> (pan leukocyte, feline) and <t>MHC</t> II. Both feline ( g ) and human ( h ) ASCs undergo osteogenic differentiation. Cell size presented as mean and standard error. * P
    Anti Mhc Class Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mhc class ii/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mhc class ii - by Bioz Stars, 2021-09
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    97
    BioLegend mhcii
    Phenotypic characterization of duodenal macrophage in response to Giardia infection. CX3CR1-GFP mice were infected with 1 × 10 6 Giardia trophozoites on day 0 and euthanized on day 7. Staining for <t>F4/80</t> is shown in combination with CD11b ( A ), CD11c ( C ), Ly6C ( D ), and <t>MHCII</t> ( E ). CX3CR1 expression was assessed through the detection of endogenous GFP. Cells are gated on live, CD45 + , Ly6G − , Siglec-F − cells. (A) F4/80 and CD11b staining was examined on cells from duodenum and jejunum of uninfected and infected mice; and (B) the comparative cell frequencies and total numbers of F4/80 + , CD11b + macrophages (in the red boxes in (A) are shown for individual mice. (C–E) Surface expression of CD11c (C), Ly6C (D), and MHCII (E) of F4/80 + , CD11b + macrophages are shown as red dots, with black dots representing F4/80 − , CD11b − cells. All flow cytometry plots are shown from one individual mouse from the uninfected and infected groups and are representative of three mice per group. Flow cytometry density plots were adjusted with smoothing = 20. Flow cytometry data are representative of two independent experiments using CX3CR1-GFP mice. Error bars represent mean ± SEM. Each circle represents data from one mouse with * p
    Mhcii, supplied by BioLegend, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mhcii/product/BioLegend
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    94
    Thermo Fisher anti mhc ii
    Expression of <t>MHC-I,</t> MHC-II, and CD80/86 on putative cDC1, cDC2, and <t>CD14</t> + DCs after stimulation by TLR3, TLR4, and TLR7 agonists (10 μg/ml poly I:C, 1 μg/ml LPS, and 10 μg/ml gardiquimod, respectively) for 18 h or unstimulated as controls. Unstimulated control, gray histogram; poly I:C, red histogram; LPS, blue histogram; gardiquimod, green histogram. Data are representative of three pigs.
    Anti Mhc Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mhc ii/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mhc ii - by Bioz Stars, 2021-09
    94/100 stars
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    Image Search Results


    Human and feline ASCs possess typical MSC characteristics. Feline ( a ) and human ( b ) ASCs adhere to plastic and have a spindle, fibroblast morphology in culture. However, human ASCs are significantly larger than feline ASCs when adherent ( c ) and in suspension ( d ). Feline ( e ) and human ( f ) ASCs both have positive surface expression of CD44, CD90, and CD105, and are negative for surface expression of CD45 (pan leukocyte, human) or CD18 (pan leukocyte, feline) and MHC II. Both feline ( g ) and human ( h ) ASCs undergo osteogenic differentiation. Cell size presented as mean and standard error. * P

    Journal: Stem Cell Research & Therapy

    Article Title: Human and feline adipose-derived mesenchymal stem cells have comparable phenotype, immunomodulatory functions, and transcriptome

    doi: 10.1186/s13287-017-0528-z

    Figure Lengend Snippet: Human and feline ASCs possess typical MSC characteristics. Feline ( a ) and human ( b ) ASCs adhere to plastic and have a spindle, fibroblast morphology in culture. However, human ASCs are significantly larger than feline ASCs when adherent ( c ) and in suspension ( d ). Feline ( e ) and human ( f ) ASCs both have positive surface expression of CD44, CD90, and CD105, and are negative for surface expression of CD45 (pan leukocyte, human) or CD18 (pan leukocyte, feline) and MHC II. Both feline ( g ) and human ( h ) ASCs undergo osteogenic differentiation. Cell size presented as mean and standard error. * P

    Article Snippet: All human antibodies were purchased from BD Biosciences and feline antibodies were purchased from the Leukocyte Antigen Biology Laboratory, UCD, unless otherwise indicated (MHC II (clone 42.3), CD18 (clone FE3.9 F2), CD90 (clone CA1.4G8), CD44 (clone IM7; BioLegend, San Diego, CA, USA), and CD105 (SN6; eBioscience, SanDiego, CA, USA).

    Techniques: Expressing

    Phenotypic characterization of duodenal macrophage in response to Giardia infection. CX3CR1-GFP mice were infected with 1 × 10 6 Giardia trophozoites on day 0 and euthanized on day 7. Staining for F4/80 is shown in combination with CD11b ( A ), CD11c ( C ), Ly6C ( D ), and MHCII ( E ). CX3CR1 expression was assessed through the detection of endogenous GFP. Cells are gated on live, CD45 + , Ly6G − , Siglec-F − cells. (A) F4/80 and CD11b staining was examined on cells from duodenum and jejunum of uninfected and infected mice; and (B) the comparative cell frequencies and total numbers of F4/80 + , CD11b + macrophages (in the red boxes in (A) are shown for individual mice. (C–E) Surface expression of CD11c (C), Ly6C (D), and MHCII (E) of F4/80 + , CD11b + macrophages are shown as red dots, with black dots representing F4/80 − , CD11b − cells. All flow cytometry plots are shown from one individual mouse from the uninfected and infected groups and are representative of three mice per group. Flow cytometry density plots were adjusted with smoothing = 20. Flow cytometry data are representative of two independent experiments using CX3CR1-GFP mice. Error bars represent mean ± SEM. Each circle represents data from one mouse with * p

    Journal: ImmunoHorizons

    Article Title: Proliferation of Resident Macrophages Is Dispensable for Protection during Giardia duodenalis Infections

    doi: 10.4049/immunohorizons.1900041

    Figure Lengend Snippet: Phenotypic characterization of duodenal macrophage in response to Giardia infection. CX3CR1-GFP mice were infected with 1 × 10 6 Giardia trophozoites on day 0 and euthanized on day 7. Staining for F4/80 is shown in combination with CD11b ( A ), CD11c ( C ), Ly6C ( D ), and MHCII ( E ). CX3CR1 expression was assessed through the detection of endogenous GFP. Cells are gated on live, CD45 + , Ly6G − , Siglec-F − cells. (A) F4/80 and CD11b staining was examined on cells from duodenum and jejunum of uninfected and infected mice; and (B) the comparative cell frequencies and total numbers of F4/80 + , CD11b + macrophages (in the red boxes in (A) are shown for individual mice. (C–E) Surface expression of CD11c (C), Ly6C (D), and MHCII (E) of F4/80 + , CD11b + macrophages are shown as red dots, with black dots representing F4/80 − , CD11b − cells. All flow cytometry plots are shown from one individual mouse from the uninfected and infected groups and are representative of three mice per group. Flow cytometry density plots were adjusted with smoothing = 20. Flow cytometry data are representative of two independent experiments using CX3CR1-GFP mice. Error bars represent mean ± SEM. Each circle represents data from one mouse with * p

    Article Snippet: Cells were FcR blocked with TruStain FcX (anti-mouse CD16/32 Ab) (BioLegend) for 15 min at room temperature in the dark and then immediately stained for cellular surface markers with fluorophore-conjugated Abs against F4/80, CD11b, CD11c, MHCII, Ly6C, CD3, CD4, and CD8 (all BioLegend) for 20 min at 4°C.

    Techniques: Infection, Mouse Assay, Staining, Expressing, Flow Cytometry

    Expression of MHC-I, MHC-II, and CD80/86 on putative cDC1, cDC2, and CD14 + DCs after stimulation by TLR3, TLR4, and TLR7 agonists (10 μg/ml poly I:C, 1 μg/ml LPS, and 10 μg/ml gardiquimod, respectively) for 18 h or unstimulated as controls. Unstimulated control, gray histogram; poly I:C, red histogram; LPS, blue histogram; gardiquimod, green histogram. Data are representative of three pigs.

    Journal: Frontiers in Immunology

    Article Title: Development of Pig Conventional Dendritic Cells From Bone Marrow Hematopoietic Cells in vitro

    doi: 10.3389/fimmu.2020.553859

    Figure Lengend Snippet: Expression of MHC-I, MHC-II, and CD80/86 on putative cDC1, cDC2, and CD14 + DCs after stimulation by TLR3, TLR4, and TLR7 agonists (10 μg/ml poly I:C, 1 μg/ml LPS, and 10 μg/ml gardiquimod, respectively) for 18 h or unstimulated as controls. Unstimulated control, gray histogram; poly I:C, red histogram; LPS, blue histogram; gardiquimod, green histogram. Data are representative of three pigs.

    Article Snippet: Finally, cells were stained with anti-CD14 FITC, anti-MHC-II conjugated to anti-mouse IgG2b Zenon Alexa Fluor 647 (Thermo Fisher Scientific, Spain), and anti-CD172a conjugated to LYNX Rapid RPE (Bio-Rad, Spain).

    Techniques: Expressing

    Phenotype of Flt3 ligand-derived dendritic cells (Flt3L-DCs). Cells were stained for multi-color flow cytometry. (A) Illustrative density plots show the gating strategy: singlets → excluding cell debris → selecting CADM1 + population and divided by CD14 → identify cDC1-putative and cDC2-putative populations based on MHC-II + and different expression of cD172a. Three putative populations were identified with cDC1-putative population gated as CADM1 + CD14 – MHC-II + CD172a – / lo , cDC2-putative population as CADM1 + CD14 – MHC-II + CD172a + , and plus a CD14 + population (CADM1 + CD14 + MHC-II + CD172a + ) without classification. (B) Expression of phenotypic markers CD1, CD163, DEC205, CD11R1, CD11R3, CD33, and CD80/86 on the defined cDC1-putative, cDC1-putative, and CD14 + populations was assessed by flow cytometry. The filled histogram represents the FMO control. The density plots and histograms shown are illustrative for one but represent four pigs.

    Journal: Frontiers in Immunology

    Article Title: Development of Pig Conventional Dendritic Cells From Bone Marrow Hematopoietic Cells in vitro

    doi: 10.3389/fimmu.2020.553859

    Figure Lengend Snippet: Phenotype of Flt3 ligand-derived dendritic cells (Flt3L-DCs). Cells were stained for multi-color flow cytometry. (A) Illustrative density plots show the gating strategy: singlets → excluding cell debris → selecting CADM1 + population and divided by CD14 → identify cDC1-putative and cDC2-putative populations based on MHC-II + and different expression of cD172a. Three putative populations were identified with cDC1-putative population gated as CADM1 + CD14 – MHC-II + CD172a – / lo , cDC2-putative population as CADM1 + CD14 – MHC-II + CD172a + , and plus a CD14 + population (CADM1 + CD14 + MHC-II + CD172a + ) without classification. (B) Expression of phenotypic markers CD1, CD163, DEC205, CD11R1, CD11R3, CD33, and CD80/86 on the defined cDC1-putative, cDC1-putative, and CD14 + populations was assessed by flow cytometry. The filled histogram represents the FMO control. The density plots and histograms shown are illustrative for one but represent four pigs.

    Article Snippet: Finally, cells were stained with anti-CD14 FITC, anti-MHC-II conjugated to anti-mouse IgG2b Zenon Alexa Fluor 647 (Thermo Fisher Scientific, Spain), and anti-CD172a conjugated to LYNX Rapid RPE (Bio-Rad, Spain).

    Techniques: Derivative Assay, Staining, Flow Cytometry, Expressing