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Image Search Results

Journal: PLoS ONE
Article Title: Overexpression of the Large-Conductance, Ca 2+ -Activated K + (BK) Channel Shortens Action Potential Duration in HL-1 Cardiomyocytes
doi: 10.1371/journal.pone.0130588
Figure Lengend Snippet: A) Examples of Western blots comparing ion channel expression levels between HL-1 cells transfected with Null or +hBKα for 48 h. Pore-forming α-subunits of voltage-gated K + channels (Kv4.3, Kv7.1, Kv11.1) native to HL-1 cells and voltage-gated Na + (Na v 1.5) and L-type Ca 2+ (Ca v 1.2) channels were probed. Molecular mass markers for each blot (in kD) are indicated on the right column. B) Density of immunoreactive bands were averaged from 5 separate HL-1 cell cultures transfected with Null or +hBKα (n = 5). * = significant difference from Null, P<0.05. See and for complete Western blots of the data described in this Fig.
Article Snippet: Western blot analyses were performed using the following primary antibodies: anti-Flag (Sigma-Aldrich), anti-Kv4.3 (Alomone, Jerusalem, Israel),
Techniques: Western Blot, Expressing, Transfection