anti inos antibody Santa Cruz Biotechnology Search Results


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  • 99
    Millipore antibodies against inos
    Antibodies Against Inos, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti inducible nitric oxide synthase inos
    Dose-dependent effect of barley sprouts extract on nitric oxide (NO) generation and protein expression of inducible nitric oxide <t>synthase</t> <t>(iNOS)</t> and cyclooxygenase (COX)-2 in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. ( A ) Cell viability was determined after treatment with barley sprouts extract for 24 h. Cells were pretreated with barley sprouts extract for 1 h before treatment with 200 ng/mL LPS for 24 h; ( B ) NO generation in medium and ( C ) protein expression of iNOS and COX2 in whole cell lysates; ( D ) Quantitative analysis of blots. Each value is mean ± standard deviation (SD) of triplicates in three independent experiments. Values with different letters are significantly different by analysis of variance (ANOVA) followed by Newman–Keuls multiple range test ( p
    Anti Inducible Nitric Oxide Synthase Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti inos
    Manganese exposure induced NLRP3 inflammasome activation in microglial cells in vivo ( A ) Western Blot analysis of the NLRP3 and <t>iNOS</t> expression in wild-type microglial cells treated with Mn and αSyn Agg as indicated. Blots (left) are representative of 3 independent experiments. Normalized band intensity data (right) are means ± SEM from all experiments. ( B ) Luminex analysis of IL-1β production by wild-type microglial cells treated with Mn and αSyn Agg as indicated. Data are means ± SEM pooled from 4 independent experiments. ( C ) qRT-PCR analysis of NLRP3, NLRC4, and AIM2 mRNA expression in the striata of C57BL mice exposed to Mn for 30 days. Data are means ± SEM pooled from 5 mice from 3 experiments. ( D ) Western blot analysis of <t>Caspase</t> 1 cleavage and IL-1β maturation in lysates from striatum samples from mice treated as indicated. Blots (upper) are representative of 6 mice from 3 experiments. Normalized band intensity data (lower) are means ± SEM from all experiments. ( E ) Immunofluorescence microscopy analysis of NLRP3 abundance in IBA1-positive microglial cells in the striatal region of mice treated as indicated. Images are representative of 3 mice from 3 experiments. Scale bar, 15 μm. *P
    Anti Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology polyclonal rabbit anti inducible nitric oxide synthase inos
    Manganese exposure induced NLRP3 inflammasome activation in microglial cells in vivo ( A ) Western Blot analysis of the NLRP3 and <t>iNOS</t> expression in wild-type microglial cells treated with Mn and αSyn Agg as indicated. Blots (left) are representative of 3 independent experiments. Normalized band intensity data (right) are means ± SEM from all experiments. ( B ) Luminex analysis of IL-1β production by wild-type microglial cells treated with Mn and αSyn Agg as indicated. Data are means ± SEM pooled from 4 independent experiments. ( C ) qRT-PCR analysis of NLRP3, NLRC4, and AIM2 mRNA expression in the striata of C57BL mice exposed to Mn for 30 days. Data are means ± SEM pooled from 5 mice from 3 experiments. ( D ) Western blot analysis of <t>Caspase</t> 1 cleavage and IL-1β maturation in lysates from striatum samples from mice treated as indicated. Blots (upper) are representative of 6 mice from 3 experiments. Normalized band intensity data (lower) are means ± SEM from all experiments. ( E ) Immunofluorescence microscopy analysis of NLRP3 abundance in IBA1-positive microglial cells in the striatal region of mice treated as indicated. Images are representative of 3 mice from 3 experiments. Scale bar, 15 μm. *P
    Polyclonal Rabbit Anti Inducible Nitric Oxide Synthase Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti inducible nitric oxide synthase anti inos isoform nitros oxide
    Manganese exposure induced NLRP3 inflammasome activation in microglial cells in vivo ( A ) Western Blot analysis of the NLRP3 and <t>iNOS</t> expression in wild-type microglial cells treated with Mn and αSyn Agg as indicated. Blots (left) are representative of 3 independent experiments. Normalized band intensity data (right) are means ± SEM from all experiments. ( B ) Luminex analysis of IL-1β production by wild-type microglial cells treated with Mn and αSyn Agg as indicated. Data are means ± SEM pooled from 4 independent experiments. ( C ) qRT-PCR analysis of NLRP3, NLRC4, and AIM2 mRNA expression in the striata of C57BL mice exposed to Mn for 30 days. Data are means ± SEM pooled from 5 mice from 3 experiments. ( D ) Western blot analysis of <t>Caspase</t> 1 cleavage and IL-1β maturation in lysates from striatum samples from mice treated as indicated. Blots (upper) are representative of 6 mice from 3 experiments. Normalized band intensity data (lower) are means ± SEM from all experiments. ( E ) Immunofluorescence microscopy analysis of NLRP3 abundance in IBA1-positive microglial cells in the striatal region of mice treated as indicated. Images are representative of 3 mice from 3 experiments. Scale bar, 15 μm. *P
    Anti Inducible Nitric Oxide Synthase Anti Inos Isoform Nitros Oxide, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology rabbit anti inducible nitric oxide synthase inos
    Manganese exposure induced NLRP3 inflammasome activation in microglial cells in vivo ( A ) Western Blot analysis of the NLRP3 and <t>iNOS</t> expression in wild-type microglial cells treated with Mn and αSyn Agg as indicated. Blots (left) are representative of 3 independent experiments. Normalized band intensity data (right) are means ± SEM from all experiments. ( B ) Luminex analysis of IL-1β production by wild-type microglial cells treated with Mn and αSyn Agg as indicated. Data are means ± SEM pooled from 4 independent experiments. ( C ) qRT-PCR analysis of NLRP3, NLRC4, and AIM2 mRNA expression in the striata of C57BL mice exposed to Mn for 30 days. Data are means ± SEM pooled from 5 mice from 3 experiments. ( D ) Western blot analysis of <t>Caspase</t> 1 cleavage and IL-1β maturation in lysates from striatum samples from mice treated as indicated. Blots (upper) are representative of 6 mice from 3 experiments. Normalized band intensity data (lower) are means ± SEM from all experiments. ( E ) Immunofluorescence microscopy analysis of NLRP3 abundance in IBA1-positive microglial cells in the striatal region of mice treated as indicated. Images are representative of 3 mice from 3 experiments. Scale bar, 15 μm. *P
    Rabbit Anti Inducible Nitric Oxide Synthase Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 87/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology phycoerythrin pe conjugated anti inducible nitric oxide synthase
    Manganese exposure induced NLRP3 inflammasome activation in microglial cells in vivo ( A ) Western Blot analysis of the NLRP3 and <t>iNOS</t> expression in wild-type microglial cells treated with Mn and αSyn Agg as indicated. Blots (left) are representative of 3 independent experiments. Normalized band intensity data (right) are means ± SEM from all experiments. ( B ) Luminex analysis of IL-1β production by wild-type microglial cells treated with Mn and αSyn Agg as indicated. Data are means ± SEM pooled from 4 independent experiments. ( C ) qRT-PCR analysis of NLRP3, NLRC4, and AIM2 mRNA expression in the striata of C57BL mice exposed to Mn for 30 days. Data are means ± SEM pooled from 5 mice from 3 experiments. ( D ) Western blot analysis of <t>Caspase</t> 1 cleavage and IL-1β maturation in lysates from striatum samples from mice treated as indicated. Blots (upper) are representative of 6 mice from 3 experiments. Normalized band intensity data (lower) are means ± SEM from all experiments. ( E ) Immunofluorescence microscopy analysis of NLRP3 abundance in IBA1-positive microglial cells in the striatal region of mice treated as indicated. Images are representative of 3 mice from 3 experiments. Scale bar, 15 μm. *P
    Phycoerythrin Pe Conjugated Anti Inducible Nitric Oxide Synthase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 87/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology mouse anti inos monoclonal antibody
    Effect of P2Y12R on LPS-stimulated BV-2 microglial morphological changes and inflammatory cytokine production. a Double immunofluorescence staining images of <t>iNOS</t> with Iba-1 in BV-2 cells stimulated with LPS and pretreated with MRS2395/clopidogrel (1.8 μM). Scale bar: 20 μm. b Representative Western blot showing iNOS expression in LPS-stimulated BV-2 cells pretreated with the <t>P2Y12</t> antagonists MRS2395 (20 μM) and clopidogrel (0.18 and 1.8 μM). The band intensity is presented relative to that of β-actin. Data are presented as mean ± SEM of three independent experiments; ** p
    Mouse Anti Inos Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology goat polyclonal anti inducible nitric oxide synthase
    Effect of P2Y12R on LPS-stimulated BV-2 microglial morphological changes and inflammatory cytokine production. a Double immunofluorescence staining images of <t>iNOS</t> with Iba-1 in BV-2 cells stimulated with LPS and pretreated with MRS2395/clopidogrel (1.8 μM). Scale bar: 20 μm. b Representative Western blot showing iNOS expression in LPS-stimulated BV-2 cells pretreated with the <t>P2Y12</t> antagonists MRS2395 (20 μM) and clopidogrel (0.18 and 1.8 μM). The band intensity is presented relative to that of β-actin. Data are presented as mean ± SEM of three independent experiments; ** p
    Goat Polyclonal Anti Inducible Nitric Oxide Synthase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti inos rabbit polyclonal
    Inhibition of inducible nitric oxide synthase <t>(iNOS)</t> and cyclooxygenase-2 (COX-2) expression by Zuonin B. RAW264.7 cells were pretreated with different concentrations of Zuonin B for 1 h and stimulated with LPS (1 μ g/mL) for a further 24 h. Equal amounts of protein in cell lysates were electrophoresed, and the protein expression levels of iNOS (a) and COX-2 (b) determined using specific antibodies for iNOS and COX-2. The respective levels of β -actin were used to confirm equal amounts of protein loading for electrophoresis. Interleukin COX-2 and iNOS mRNA levels in lung tissue, (c) Cells were cultured for 24 h with LPS (1 μ g/mL), fixed, permeabilized, and incubated with rabbit <t>polyclonal</t> anti-iNOS and COX-2 antibody, followed by Texas-red conjugated anti-rabbit Ig (red). The nuclei of the corresponding cells were visualized with 4′,6-diamidino-2-phenylindole (DAPI) (magnification ×400). NC: untreated control cells; LPS: LPS only treatment.
    Anti Inos Rabbit Polyclonal, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology mouse anti human inducible nitric oxide synthase inos
    Inhibition of inducible nitric oxide synthase <t>(iNOS)</t> and cyclooxygenase-2 (COX-2) expression by Zuonin B. RAW264.7 cells were pretreated with different concentrations of Zuonin B for 1 h and stimulated with LPS (1 μ g/mL) for a further 24 h. Equal amounts of protein in cell lysates were electrophoresed, and the protein expression levels of iNOS (a) and COX-2 (b) determined using specific antibodies for iNOS and COX-2. The respective levels of β -actin were used to confirm equal amounts of protein loading for electrophoresis. Interleukin COX-2 and iNOS mRNA levels in lung tissue, (c) Cells were cultured for 24 h with LPS (1 μ g/mL), fixed, permeabilized, and incubated with rabbit <t>polyclonal</t> anti-iNOS and COX-2 antibody, followed by Texas-red conjugated anti-rabbit Ig (red). The nuclei of the corresponding cells were visualized with 4′,6-diamidino-2-phenylindole (DAPI) (magnification ×400). NC: untreated control cells; LPS: LPS only treatment.
    Mouse Anti Human Inducible Nitric Oxide Synthase Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology polyclonal rabbit anti rat inducible nitric oxide synthase inos antibody
    Inhibition of inducible nitric oxide synthase <t>(iNOS)</t> and cyclooxygenase-2 (COX-2) expression by Zuonin B. RAW264.7 cells were pretreated with different concentrations of Zuonin B for 1 h and stimulated with LPS (1 μ g/mL) for a further 24 h. Equal amounts of protein in cell lysates were electrophoresed, and the protein expression levels of iNOS (a) and COX-2 (b) determined using specific antibodies for iNOS and COX-2. The respective levels of β -actin were used to confirm equal amounts of protein loading for electrophoresis. Interleukin COX-2 and iNOS mRNA levels in lung tissue, (c) Cells were cultured for 24 h with LPS (1 μ g/mL), fixed, permeabilized, and incubated with rabbit <t>polyclonal</t> anti-iNOS and COX-2 antibody, followed by Texas-red conjugated anti-rabbit Ig (red). The nuclei of the corresponding cells were visualized with 4′,6-diamidino-2-phenylindole (DAPI) (magnification ×400). NC: untreated control cells; LPS: LPS only treatment.
    Polyclonal Rabbit Anti Rat Inducible Nitric Oxide Synthase Inos Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti mouse inducible nitric oxide synthases
    Inhibition of inducible nitric oxide synthase <t>(iNOS)</t> and cyclooxygenase-2 (COX-2) expression by Zuonin B. RAW264.7 cells were pretreated with different concentrations of Zuonin B for 1 h and stimulated with LPS (1 μ g/mL) for a further 24 h. Equal amounts of protein in cell lysates were electrophoresed, and the protein expression levels of iNOS (a) and COX-2 (b) determined using specific antibodies for iNOS and COX-2. The respective levels of β -actin were used to confirm equal amounts of protein loading for electrophoresis. Interleukin COX-2 and iNOS mRNA levels in lung tissue, (c) Cells were cultured for 24 h with LPS (1 μ g/mL), fixed, permeabilized, and incubated with rabbit <t>polyclonal</t> anti-iNOS and COX-2 antibody, followed by Texas-red conjugated anti-rabbit Ig (red). The nuclei of the corresponding cells were visualized with 4′,6-diamidino-2-phenylindole (DAPI) (magnification ×400). NC: untreated control cells; LPS: LPS only treatment.
    Anti Mouse Inducible Nitric Oxide Synthases, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology mouse anti inducible nitric oxide synthases inos
    Inhibition of inducible nitric oxide synthase <t>(iNOS)</t> and cyclooxygenase-2 (COX-2) expression by Zuonin B. RAW264.7 cells were pretreated with different concentrations of Zuonin B for 1 h and stimulated with LPS (1 μ g/mL) for a further 24 h. Equal amounts of protein in cell lysates were electrophoresed, and the protein expression levels of iNOS (a) and COX-2 (b) determined using specific antibodies for iNOS and COX-2. The respective levels of β -actin were used to confirm equal amounts of protein loading for electrophoresis. Interleukin COX-2 and iNOS mRNA levels in lung tissue, (c) Cells were cultured for 24 h with LPS (1 μ g/mL), fixed, permeabilized, and incubated with rabbit <t>polyclonal</t> anti-iNOS and COX-2 antibody, followed by Texas-red conjugated anti-rabbit Ig (red). The nuclei of the corresponding cells were visualized with 4′,6-diamidino-2-phenylindole (DAPI) (magnification ×400). NC: untreated control cells; LPS: LPS only treatment.
    Mouse Anti Inducible Nitric Oxide Synthases Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology polyclonal rabbit anti mouse inducible nitric oxide synthase inos
    Inhibiting TNFα and <t>iNOS</t> production by human marrow stromal cell treatment in SOD1 transgenic mice. Lumbar spinal cord tissues from wild-type mice, PBS-treated and human marrow stromal cell (hMSC)-treated transgenic mice at the age of 11 weeks are assayed for inducible nitric oxide <t>synthase</t> (iNOS) protein expression by Immunoblot and TNFα level by ELISA. ( A ) Representative immunoblots for iNOS and β-actin of the lumbar spinal cord from PBS-treated transgenic mice (1), wild-type mice (2) and hMSC-treated transgenic mice (3). ( B ) Quantitative evaluation through optical densitometry of iNOS blot and relation to β-actin blot. The levels of iNOS in Cu/Zn superoxide dismutase 1 (SOD1) transgenic mice receiving hMSC transplantation were significantly reduced compared with those in vehicle-treated mice (* P
    Polyclonal Rabbit Anti Mouse Inducible Nitric Oxide Synthase Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti inducible nitric oxide no synthase synthase inos igg
    Inhibiting TNFα and <t>iNOS</t> production by human marrow stromal cell treatment in SOD1 transgenic mice. Lumbar spinal cord tissues from wild-type mice, PBS-treated and human marrow stromal cell (hMSC)-treated transgenic mice at the age of 11 weeks are assayed for inducible nitric oxide <t>synthase</t> (iNOS) protein expression by Immunoblot and TNFα level by ELISA. ( A ) Representative immunoblots for iNOS and β-actin of the lumbar spinal cord from PBS-treated transgenic mice (1), wild-type mice (2) and hMSC-treated transgenic mice (3). ( B ) Quantitative evaluation through optical densitometry of iNOS blot and relation to β-actin blot. The levels of iNOS in Cu/Zn superoxide dismutase 1 (SOD1) transgenic mice receiving hMSC transplantation were significantly reduced compared with those in vehicle-treated mice (* P
    Anti Inducible Nitric Oxide No Synthase Synthase Inos Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology rabbit igg anti mouse inducible nitric oxide synthase inos enzyme
    Th17-associated cytokines regulate the IFN-γ production and inducible nitric oxide <t>synthase</t> enzyme <t>(iNOS)</t> expression during the experiment of virulent yeast strain 18 of Paracoccidioides brasiliensis (Pb18) infection. (A,B) C57BL/6, IL-6 −/− , IL-23 −/− , and IL-17 receptor A (IL-17RA) −/− mice were infected (intravenously) with 1 × 10 6 Pb18 yeast cells. (A) IFN-γ levels were measured by enzyme-linked immunosorbent assay in the lung homogenate at 15 and 30 days postinfection (dpi). (B) Immunohistochemistry was performed in lung tissue to analyze the iNOS expression at 30 dpi. (C) The percentage of the pulmonary area containing iNOS + cells is indicated. Lung sections were stained for iNOS at 30 dpi using immunohistochemistry. Scale bar: 50 µm. Similar results were obtained in three independent experiments. Data represent the mean ± SEM of five mice. Uninfected mice are represented by the dashed line. * p
    Rabbit Igg Anti Mouse Inducible Nitric Oxide Synthase Inos Enzyme, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology goat monoclonal anti inducible nitric oxide synthase antibody
    Th17-associated cytokines regulate the IFN-γ production and inducible nitric oxide <t>synthase</t> enzyme <t>(iNOS)</t> expression during the experiment of virulent yeast strain 18 of Paracoccidioides brasiliensis (Pb18) infection. (A,B) C57BL/6, IL-6 −/− , IL-23 −/− , and IL-17 receptor A (IL-17RA) −/− mice were infected (intravenously) with 1 × 10 6 Pb18 yeast cells. (A) IFN-γ levels were measured by enzyme-linked immunosorbent assay in the lung homogenate at 15 and 30 days postinfection (dpi). (B) Immunohistochemistry was performed in lung tissue to analyze the iNOS expression at 30 dpi. (C) The percentage of the pulmonary area containing iNOS + cells is indicated. Lung sections were stained for iNOS at 30 dpi using immunohistochemistry. Scale bar: 50 µm. Similar results were obtained in three independent experiments. Data represent the mean ± SEM of five mice. Uninfected mice are represented by the dashed line. * p
    Goat Monoclonal Anti Inducible Nitric Oxide Synthase Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti inos pe
    Role of <t>iNOS</t> in neutrophil apoptosis. ( a ) Neutrophil apoptosis was measured by Annexin V-PI labelling in scrambled, iNOS and <t>nNOS</t> silenced human PMNs after 12 h of incubation ( N =5). ( b ) Caspase-3 activity assay was performed in scrambled, iNOS and nNOS silenced human PMNs ( N =3). ( c ) Annexin V-PI labelling of BMDN from WT, iNOS and nNOS KO mice after 18 h of in vitro culture ( N =5). ( d ) Caspase-3 activity was monitored in BMDN from WT, iNOS and nNOS KO mice using caspase-3 specific fluorogenic substrate acetyl-Asp-Glu-Val-Asp-7-amino-4 methylcoumarin (150 μ M; N =5). Mitochondrial membrane potential in control and iNOS silenced human PMNs ( e ) and WT and iNOS KO BMDN ( f ) ( N =5).Data represent mean±S.E.M. of three to five independent experiments. * P
    Anti Inos Pe, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology polyclonal anti inos
    Role of <t>iNOS</t> in neutrophil apoptosis. ( a ) Neutrophil apoptosis was measured by Annexin V-PI labelling in scrambled, iNOS and <t>nNOS</t> silenced human PMNs after 12 h of incubation ( N =5). ( b ) Caspase-3 activity assay was performed in scrambled, iNOS and nNOS silenced human PMNs ( N =3). ( c ) Annexin V-PI labelling of BMDN from WT, iNOS and nNOS KO mice after 18 h of in vitro culture ( N =5). ( d ) Caspase-3 activity was monitored in BMDN from WT, iNOS and nNOS KO mice using caspase-3 specific fluorogenic substrate acetyl-Asp-Glu-Val-Asp-7-amino-4 methylcoumarin (150 μ M; N =5). Mitochondrial membrane potential in control and iNOS silenced human PMNs ( e ) and WT and iNOS KO BMDN ( f ) ( N =5).Data represent mean±S.E.M. of three to five independent experiments. * P
    Polyclonal Anti Inos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology rabbit anti mouse inducible nitric oxide synthase inos
    Role of <t>iNOS</t> in neutrophil apoptosis. ( a ) Neutrophil apoptosis was measured by Annexin V-PI labelling in scrambled, iNOS and <t>nNOS</t> silenced human PMNs after 12 h of incubation ( N =5). ( b ) Caspase-3 activity assay was performed in scrambled, iNOS and nNOS silenced human PMNs ( N =3). ( c ) Annexin V-PI labelling of BMDN from WT, iNOS and nNOS KO mice after 18 h of in vitro culture ( N =5). ( d ) Caspase-3 activity was monitored in BMDN from WT, iNOS and nNOS KO mice using caspase-3 specific fluorogenic substrate acetyl-Asp-Glu-Val-Asp-7-amino-4 methylcoumarin (150 μ M; N =5). Mitochondrial membrane potential in control and iNOS silenced human PMNs ( e ) and WT and iNOS KO BMDN ( f ) ( N =5).Data represent mean±S.E.M. of three to five independent experiments. * P
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    Downregulation of inflammation. (a1) Cells were pretreated with different concentrations of FFSO for 30 minutes and then stimulated with LPS (1 microg/mL) for 12 hours. Ratio of TNF- α to GAPDH expression in cells was determined by real-time PCR, as described in Section 2 . (a2) Cells were pretreated with the indicated concentrations of FFSO for 2 hours and then stimulated with LPS (1 microg/mL) for 1 hour, after which nuclear extracts were prepared. Detection of NF- κ B-binding activities was performed as described in Section 2 . This experiment was repeated two times with similar results. (a3) Ratio of COX-2 to GAPDH expression on cells same as (a1). (a4) Cells were pretreated with different concentrations of FFSO for 30 minutes and then stimulated with LPS (1 microg/mL) for another 24 hours. Expression of <t>iNOS</t> and COX-2 proteins was detected by Western blotting using specific anti-iNOS and anti-COX-2 antibodies. β -Actin protein was used as an internal control. Expression of TNF- α , NF-kB <t>p65,</t> iNOS, and COX-2 (arrow indicates dark brown) was induced by D. farinae in the AE group. These positive reactions in the AT group were remarkably decreased compared with those in the AE group (immunohistochemistry; Bar size, 50 μ m). Abbreviations are the same as in Figure 1 . ∗ P
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    Santa Cruz Biotechnology anti inos m 19
    Downregulation of inflammation. (a1) Cells were pretreated with different concentrations of FFSO for 30 minutes and then stimulated with LPS (1 microg/mL) for 12 hours. Ratio of TNF- α to GAPDH expression in cells was determined by real-time PCR, as described in Section 2 . (a2) Cells were pretreated with the indicated concentrations of FFSO for 2 hours and then stimulated with LPS (1 microg/mL) for 1 hour, after which nuclear extracts were prepared. Detection of NF- κ B-binding activities was performed as described in Section 2 . This experiment was repeated two times with similar results. (a3) Ratio of COX-2 to GAPDH expression on cells same as (a1). (a4) Cells were pretreated with different concentrations of FFSO for 30 minutes and then stimulated with LPS (1 microg/mL) for another 24 hours. Expression of <t>iNOS</t> and COX-2 proteins was detected by Western blotting using specific anti-iNOS and anti-COX-2 antibodies. β -Actin protein was used as an internal control. Expression of TNF- α , NF-kB <t>p65,</t> iNOS, and COX-2 (arrow indicates dark brown) was induced by D. farinae in the AE group. These positive reactions in the AT group were remarkably decreased compared with those in the AE group (immunohistochemistry; Bar size, 50 μ m). Abbreviations are the same as in Figure 1 . ∗ P
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    Santa Cruz Biotechnology rabbit anti inos
    Effects of SSM on SNCI-induced protein expression of TNF-α, IL-6, <t>iNOS,</t> and <t>COX-2</t> . The sciatic nerve was crushed for 30 s using a surgical clip. (a) Relative protein expression levels of TNF- α and IL-6. (b) Relative protein expression levels of iNOS and COX-2. Bands were detected using an enhanced chemiluminescence (ECL) detection kit. Actin was used as an internal control (46 kDa). (A) Sham operation group, (B) SNCI-induced group, (C) SNCI-induced and 0.1 g/kg SSM-treated group, (D) SNCI-induced and 1 g/kg SSM-treated group, and (E) SNCI-induced and 10 g/kg SSM-treated group. The results are presented as the means ± standard errors of the mean (SEMs). ∗ P
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    Santa Cruz Biotechnology pe conjugated anti inos
    Gr1 + (Ly6C+) inflammatory monocytes in the lamina propria express <t>iNOS,</t> TNFα, and IL-12 but not dendritic cell markers
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    Santa Cruz Biotechnology anti inos monoclonal antibody
    cSCK-pa 100 mediated silencing of <t>iNOS</t> expression by siRNA. Anti-iNOS siRNA481 (100 nM) was transfected into mouse monocyte-macrophage RAW264.7 cells using PAEA 128 - b -PS 40 cSCK-pa 100 at different N/P ratios (for N/P=1, cSCK 0.62 μg/mL),
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    cSCK-pa 100 mediated silencing of <t>iNOS</t> expression by siRNA. Anti-iNOS siRNA481 (100 nM) was transfected into mouse monocyte-macrophage RAW264.7 cells using PAEA 128 - b -PS 40 cSCK-pa 100 at different N/P ratios (for N/P=1, cSCK 0.62 μg/mL),
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    Santa Cruz Biotechnology anti inos polyclonal antibody pab
    cSCK-pa 100 mediated silencing of <t>iNOS</t> expression by siRNA. Anti-iNOS siRNA481 (100 nM) was transfected into mouse monocyte-macrophage RAW264.7 cells using PAEA 128 - b -PS 40 cSCK-pa 100 at different N/P ratios (for N/P=1, cSCK 0.62 μg/mL),
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    cSCK-pa 100 mediated silencing of <t>iNOS</t> expression by siRNA. Anti-iNOS siRNA481 (100 nM) was transfected into mouse monocyte-macrophage RAW264.7 cells using PAEA 128 - b -PS 40 cSCK-pa 100 at different N/P ratios (for N/P=1, cSCK 0.62 μg/mL),
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    Santa Cruz Biotechnology anti inos polycloncal antibody pab
    cSCK-pa 100 mediated silencing of <t>iNOS</t> expression by siRNA. Anti-iNOS siRNA481 (100 nM) was transfected into mouse monocyte-macrophage RAW264.7 cells using PAEA 128 - b -PS 40 cSCK-pa 100 at different N/P ratios (for N/P=1, cSCK 0.62 μg/mL),
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    cSCK-pa 100 mediated silencing of <t>iNOS</t> expression by siRNA. Anti-iNOS siRNA481 (100 nM) was transfected into mouse monocyte-macrophage RAW264.7 cells using PAEA 128 - b -PS 40 cSCK-pa 100 at different N/P ratios (for N/P=1, cSCK 0.62 μg/mL),
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    Image Search Results


    Dose-dependent effect of barley sprouts extract on nitric oxide (NO) generation and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. ( A ) Cell viability was determined after treatment with barley sprouts extract for 24 h. Cells were pretreated with barley sprouts extract for 1 h before treatment with 200 ng/mL LPS for 24 h; ( B ) NO generation in medium and ( C ) protein expression of iNOS and COX2 in whole cell lysates; ( D ) Quantitative analysis of blots. Each value is mean ± standard deviation (SD) of triplicates in three independent experiments. Values with different letters are significantly different by analysis of variance (ANOVA) followed by Newman–Keuls multiple range test ( p

    Journal: Nutrients

    Article Title: Barley Sprouts Extract Attenuates Alcoholic Fatty Liver Injury in Mice by Reducing Inflammatory Response

    doi: 10.3390/nu8070440

    Figure Lengend Snippet: Dose-dependent effect of barley sprouts extract on nitric oxide (NO) generation and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. ( A ) Cell viability was determined after treatment with barley sprouts extract for 24 h. Cells were pretreated with barley sprouts extract for 1 h before treatment with 200 ng/mL LPS for 24 h; ( B ) NO generation in medium and ( C ) protein expression of iNOS and COX2 in whole cell lysates; ( D ) Quantitative analysis of blots. Each value is mean ± standard deviation (SD) of triplicates in three independent experiments. Values with different letters are significantly different by analysis of variance (ANOVA) followed by Newman–Keuls multiple range test ( p

    Article Snippet: The membranes were incubated with TBST containing 5% milk and the primary antibodies against anti-inducible nitric oxide synthase (iNOS), anti-cyclooxygenase (COX)-2, and β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA).

    Techniques: Expressing, Standard Deviation

    Plasma markers of oxidative stress and nitric oxide synthase activity. Urinary excretion of the oxidative stress markers 8‐ OH ‐ dG (A), 8,12‐iso‐ iPF 2α‐ VI (B), and its metabolite 2,3‐dinor‐8‐iso iPF 2α (C) were increased in A 3 +/+ mice after UNX in combination with HS ( UNX ‐ HS ) but were not significantly changed in the A 3 −/− mice. UNX ‐ HS caused significant increase of plasma citrulline/arginine ratio in A 3 −/− mice (D) and significant reduction of plasma ornithine/arginine ratio in A 3 +/+ mice only (E). Baseline levels of both citrulline/arginine and ornithine/arginine ratios were lower in the A 3 −/− mice compared with A 3 +/+ mice. A 3 −/− mice had significantly lower plasma ADMA and SDMA levels (F and G). Although UNX ‐ HS did not influence circulating ADMA and SDMA levels, the SDMA level after UNX ‐ HS was significantly lower in A 3 −/− compared with A 3 +/+ mice. Data are shown as mean± SEM , * P

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Genetic Abrogation of Adenosine A3 Receptor Prevents Uninephrectomy and High Salt–Induced Hypertension

    doi: 10.1161/JAHA.116.003868

    Figure Lengend Snippet: Plasma markers of oxidative stress and nitric oxide synthase activity. Urinary excretion of the oxidative stress markers 8‐ OH ‐ dG (A), 8,12‐iso‐ iPF 2α‐ VI (B), and its metabolite 2,3‐dinor‐8‐iso iPF 2α (C) were increased in A 3 +/+ mice after UNX in combination with HS ( UNX ‐ HS ) but were not significantly changed in the A 3 −/− mice. UNX ‐ HS caused significant increase of plasma citrulline/arginine ratio in A 3 −/− mice (D) and significant reduction of plasma ornithine/arginine ratio in A 3 +/+ mice only (E). Baseline levels of both citrulline/arginine and ornithine/arginine ratios were lower in the A 3 −/− mice compared with A 3 +/+ mice. A 3 −/− mice had significantly lower plasma ADMA and SDMA levels (F and G). Although UNX ‐ HS did not influence circulating ADMA and SDMA levels, the SDMA level after UNX ‐ HS was significantly lower in A 3 −/− compared with A 3 +/+ mice. Data are shown as mean± SEM , * P

    Article Snippet: Membranes were treated with blocking solution (5% nonfat dry milk, 20 mmol/L Tris base, 150 mmol/L NaCl, 0.1% Tween‐20, pH 7.6) and then incubated with primary antibodies gp91phox/Nox2 (BD Biosciences); p67phox (Cell Signaling/BioNordika); or p22phox, p47phox, or inducible nitric oxide synthase (iNOS; Santa Cruz Biotechnology) overnight at 4°C.

    Techniques: Activity Assay, Mouse Assay

    Inducible nitric oxide synthase (iNOS) immunoreactivity is increased following MDMA administration. ( A – D ) Representative images (light microscopy, 40×) of iNOS immunoreactivity in the frontal cortex of rats receiving ( A ) saline (CTRL) and of rats receiving MDMA and sacrificed after ( B ) 6 h, ( C ) 16 h, and ( D ) 24 h from its administration. ( E – H ) Representative images (light microscopy, 40×) of endothelial nitric oxide synthase (eNOS) immunoreactivity in the frontal cortex of rats receiving ( E ) saline (CTRL) and of rats receiving MDMA and sacrificed after ( F ) 6 h, ( G ) 16 h, and ( H ) 24 h from its administration. ( I – L ) Representative images (light microscopy, 40×) of neuronal nitric oxide synthase (nNOS) immunoreactivity in the frontal cortex of rats receiving ( I ) saline (CTRL) and of rats receiving MDMA and sacrificed after ( J ) 6 h, ( K ) 16 h, and ( L ) 24 h from its administration. Scale bar for images in panels ( A – L ) = 50 μm. ( M – O ) Quantification of ( M ) iNOS, ( N ) eNOS, and ( O ) nNOS positive-stained cells/area analyzed in controls (CTRL) and MDMA-exposed rats, sacrificed after 6 h, 16 h, and 24 h from its administration. One-way ANOVA followed by Tukey’s post-hoc test. For iNOS: F = 9.090, *** p

    Journal: International Journal of Molecular Sciences

    Article Title: Increased iNOS and Nitrosative Stress in Dopaminergic Neurons of MDMA-Exposed Rats

    doi: 10.3390/ijms20051242

    Figure Lengend Snippet: Inducible nitric oxide synthase (iNOS) immunoreactivity is increased following MDMA administration. ( A – D ) Representative images (light microscopy, 40×) of iNOS immunoreactivity in the frontal cortex of rats receiving ( A ) saline (CTRL) and of rats receiving MDMA and sacrificed after ( B ) 6 h, ( C ) 16 h, and ( D ) 24 h from its administration. ( E – H ) Representative images (light microscopy, 40×) of endothelial nitric oxide synthase (eNOS) immunoreactivity in the frontal cortex of rats receiving ( E ) saline (CTRL) and of rats receiving MDMA and sacrificed after ( F ) 6 h, ( G ) 16 h, and ( H ) 24 h from its administration. ( I – L ) Representative images (light microscopy, 40×) of neuronal nitric oxide synthase (nNOS) immunoreactivity in the frontal cortex of rats receiving ( I ) saline (CTRL) and of rats receiving MDMA and sacrificed after ( J ) 6 h, ( K ) 16 h, and ( L ) 24 h from its administration. Scale bar for images in panels ( A – L ) = 50 μm. ( M – O ) Quantification of ( M ) iNOS, ( N ) eNOS, and ( O ) nNOS positive-stained cells/area analyzed in controls (CTRL) and MDMA-exposed rats, sacrificed after 6 h, 16 h, and 24 h from its administration. One-way ANOVA followed by Tukey’s post-hoc test. For iNOS: F = 9.090, *** p

    Article Snippet: For this study, 4 μm paraffin-embedded sections of the frontal cortex region were obtained from specimen A, by using an automized microtome (Leica, Cambridge, UK), mounted on 3-amminopropyl-triethoxysilane covered slides (Fluka, Buchs, Switzerland), and dried at 37 °C for 24 h. Brain sections were then deparaffinized through graded alcohols, subjected to epitope retrieval for 15 min and incubated for two hours at room temperature with primary antibodies, diluted in a blocking buffered serum solution containing albumin and fetal bovine serum (Sigma-Aldrich S.R.L., Milan, Italy), raised against NOX2 (1:50, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), NOX1 (1: 250, Abcam, Cambridge, UK), NOX4 (1:100, Abcam), iNOS (1:100, Santa Cruz Biotechnology), eNOS, (1:100, Santa Cruz Biotechnology), nNOS (1:150, Santa Cruz Biotechnology, Inc.), 8OHdG (1:10, JaICA, Shizuoka, Japan), NT (1:600, Santa Cruz Biotechnology, Inc.), DT1 (1:100, Abcam).

    Techniques: Light Microscopy, Staining

    Manganese exposure induced NLRP3 inflammasome activation in microglial cells in vivo ( A ) Western Blot analysis of the NLRP3 and iNOS expression in wild-type microglial cells treated with Mn and αSyn Agg as indicated. Blots (left) are representative of 3 independent experiments. Normalized band intensity data (right) are means ± SEM from all experiments. ( B ) Luminex analysis of IL-1β production by wild-type microglial cells treated with Mn and αSyn Agg as indicated. Data are means ± SEM pooled from 4 independent experiments. ( C ) qRT-PCR analysis of NLRP3, NLRC4, and AIM2 mRNA expression in the striata of C57BL mice exposed to Mn for 30 days. Data are means ± SEM pooled from 5 mice from 3 experiments. ( D ) Western blot analysis of Caspase 1 cleavage and IL-1β maturation in lysates from striatum samples from mice treated as indicated. Blots (upper) are representative of 6 mice from 3 experiments. Normalized band intensity data (lower) are means ± SEM from all experiments. ( E ) Immunofluorescence microscopy analysis of NLRP3 abundance in IBA1-positive microglial cells in the striatal region of mice treated as indicated. Images are representative of 3 mice from 3 experiments. Scale bar, 15 μm. *P

    Journal: Science signaling

    Article Title: Manganese Activates NLRP3 Inflammasome Signaling and Propagates Exosomal Release of ASC in Microglial Cells

    doi: 10.1126/scisignal.aat9900

    Figure Lengend Snippet: Manganese exposure induced NLRP3 inflammasome activation in microglial cells in vivo ( A ) Western Blot analysis of the NLRP3 and iNOS expression in wild-type microglial cells treated with Mn and αSyn Agg as indicated. Blots (left) are representative of 3 independent experiments. Normalized band intensity data (right) are means ± SEM from all experiments. ( B ) Luminex analysis of IL-1β production by wild-type microglial cells treated with Mn and αSyn Agg as indicated. Data are means ± SEM pooled from 4 independent experiments. ( C ) qRT-PCR analysis of NLRP3, NLRC4, and AIM2 mRNA expression in the striata of C57BL mice exposed to Mn for 30 days. Data are means ± SEM pooled from 5 mice from 3 experiments. ( D ) Western blot analysis of Caspase 1 cleavage and IL-1β maturation in lysates from striatum samples from mice treated as indicated. Blots (upper) are representative of 6 mice from 3 experiments. Normalized band intensity data (lower) are means ± SEM from all experiments. ( E ) Immunofluorescence microscopy analysis of NLRP3 abundance in IBA1-positive microglial cells in the striatal region of mice treated as indicated. Images are representative of 3 mice from 3 experiments. Scale bar, 15 μm. *P

    Article Snippet: The primary antibodies used are as follows: anti-caspase-1 (Adipogen, 1:1000) (AB_2490248), anti-NLRP3 (Adipogen, 1:1000), anti-iNOS (Santa Cruz, 1:1000), anti-IL-1β (R & D systems, 1:500), anti-Mfn2 (Cell Signaling, 1:1000), anti-VPS35 (Santa Cruz, 1:500), and anti-ASC (Adipogen, 1:1000) (AB_2490440).

    Techniques: Activation Assay, In Vivo, Western Blot, Expressing, Luminex, Quantitative RT-PCR, Mouse Assay, Immunofluorescence, Microscopy

    Effects of DS on phosphorylation of Akt and eNOS in brain tissues. Phosphorylation of Akt (p-Akt) and eNOS (p-eNOS) in brain tissues of saline- (Con) and DS-treated mice at 60 min after ischemia. Akt, p-Akt, eNOS, p-eNOS, iNOS, and nNOS protein levels were analyzed by Western blotting ( N = 4). DS promoted Akt and eNOS phosphorylation in both ischemic (ipsilateral, Ipsil) and nonischemic regions (contralateral, Cont) of the brain compared with control.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: The Traditional Herbal Medicine, Dangkwisoo-San, Prevents Cerebral Ischemic Injury through Nitric Oxide-Dependent Mechanisms

    doi: 10.1155/2011/718302

    Figure Lengend Snippet: Effects of DS on phosphorylation of Akt and eNOS in brain tissues. Phosphorylation of Akt (p-Akt) and eNOS (p-eNOS) in brain tissues of saline- (Con) and DS-treated mice at 60 min after ischemia. Akt, p-Akt, eNOS, p-eNOS, iNOS, and nNOS protein levels were analyzed by Western blotting ( N = 4). DS promoted Akt and eNOS phosphorylation in both ischemic (ipsilateral, Ipsil) and nonischemic regions (contralateral, Cont) of the brain compared with control.

    Article Snippet: Immunoblot analysis was performed with anti-eNOS and anti-phospho-eNOS (pS1177) antibodies (BD Biosciences, San Jose, CA), anti-Akt and anti-phospho-Akt (Ser 473) antibodies (Cell signaling, Danvers, MA), anti-nNOS and anti-iNOS antibodies (Santa Cruz Biotechnology, Santa Cruz, CA) followed by incubation with secondary antibody conjugated with horseradish peroxidase.

    Techniques: Mouse Assay, Western Blot

    Effect of P2Y12R on LPS-stimulated BV-2 microglial morphological changes and inflammatory cytokine production. a Double immunofluorescence staining images of iNOS with Iba-1 in BV-2 cells stimulated with LPS and pretreated with MRS2395/clopidogrel (1.8 μM). Scale bar: 20 μm. b Representative Western blot showing iNOS expression in LPS-stimulated BV-2 cells pretreated with the P2Y12 antagonists MRS2395 (20 μM) and clopidogrel (0.18 and 1.8 μM). The band intensity is presented relative to that of β-actin. Data are presented as mean ± SEM of three independent experiments; ** p

    Journal: Journal of Neuroinflammation

    Article Title: P2Y12 receptor mediates microglial activation via RhoA/ROCK pathway in the trigeminal nucleus caudalis in a mouse model of chronic migraine

    doi: 10.1186/s12974-019-1603-4

    Figure Lengend Snippet: Effect of P2Y12R on LPS-stimulated BV-2 microglial morphological changes and inflammatory cytokine production. a Double immunofluorescence staining images of iNOS with Iba-1 in BV-2 cells stimulated with LPS and pretreated with MRS2395/clopidogrel (1.8 μM). Scale bar: 20 μm. b Representative Western blot showing iNOS expression in LPS-stimulated BV-2 cells pretreated with the P2Y12 antagonists MRS2395 (20 μM) and clopidogrel (0.18 and 1.8 μM). The band intensity is presented relative to that of β-actin. Data are presented as mean ± SEM of three independent experiments; ** p

    Article Snippet: Coverslips were blocked with 5% goat/donkey serum containing 0.3% Triton X-100 for 30 min at room temperature, and were then incubated with following primary antibodies overnight at 4 °C: rabbit anti-P2Y12 (1:500, Anaspec Inc), goat anti-Iba1 (1:200, Abcam), and mouse anti-iNOS (1:50, Santa Cruz).

    Techniques: Double Immunofluorescence Staining, Western Blot, Expressing

    Overexpression of A20 decreases inflammation in aortic allografts while increasing apoptosis in neointimal SMC. Representative IHC photomicrographs show significantly A . Decreased VCAM-1 immunostaining in the neointimal and medial layers of rAd.A20 transduced vascular allografts at 4 weeks after transplant, as opposed to saline and rAd.βgal-treated vascular allografts; This correlated with decreased infiltration of the vascular allografts by monocytes/macrophages and neutrophils, as evaluated by immunostaining with F4/80 and Gr-1 antibodies, respectively. B. Increased iNOS immunostaining in the media of rAd.A20-treated aortic allografts correlating with C increased number of TUNEL positive (blue) neointimal SMC per mm 2 , as adjusted by Adobe scaling, when compared to saline and rAd.βgal treated allografts. Overlay of IF staining for smooth muscle cell α-actin SMA (red), Caspase 3 (green) and 4’,6-diamidino-2-phenylindole (DAPI, nuclear staining, blue) confirm that most TUNEL+ cells are neointimal SMC (yellow overlay). In A , B , and C , each bar represents mean±SE of immunostaining score, or number of F4/80, Gr-1, and TUNEL+ cells/HPF of 3-4 mice in rAd.A20-treated group and 4-7 mice in the control group. The control group represents combined saline and rAd.βgal treated vascular allografts (2-3 saline and 3-4 rAd.βgal-treated). I-intima, and M-media, original magnification X400 for VCAM-1, iNOS, TUNEL, and Caspase 3/SMA, and X200 for F4/80 and Gr-1. *p

    Journal: Transplantation

    Article Title: A20-mediated Modulation of Inflammatory and Immune Responses in Aortic Allografts and Development of Transplant Arteriosclerosis

    doi: 10.1097/TP.0b013e3182419829

    Figure Lengend Snippet: Overexpression of A20 decreases inflammation in aortic allografts while increasing apoptosis in neointimal SMC. Representative IHC photomicrographs show significantly A . Decreased VCAM-1 immunostaining in the neointimal and medial layers of rAd.A20 transduced vascular allografts at 4 weeks after transplant, as opposed to saline and rAd.βgal-treated vascular allografts; This correlated with decreased infiltration of the vascular allografts by monocytes/macrophages and neutrophils, as evaluated by immunostaining with F4/80 and Gr-1 antibodies, respectively. B. Increased iNOS immunostaining in the media of rAd.A20-treated aortic allografts correlating with C increased number of TUNEL positive (blue) neointimal SMC per mm 2 , as adjusted by Adobe scaling, when compared to saline and rAd.βgal treated allografts. Overlay of IF staining for smooth muscle cell α-actin SMA (red), Caspase 3 (green) and 4’,6-diamidino-2-phenylindole (DAPI, nuclear staining, blue) confirm that most TUNEL+ cells are neointimal SMC (yellow overlay). In A , B , and C , each bar represents mean±SE of immunostaining score, or number of F4/80, Gr-1, and TUNEL+ cells/HPF of 3-4 mice in rAd.A20-treated group and 4-7 mice in the control group. The control group represents combined saline and rAd.βgal treated vascular allografts (2-3 saline and 3-4 rAd.βgal-treated). I-intima, and M-media, original magnification X400 for VCAM-1, iNOS, TUNEL, and Caspase 3/SMA, and X200 for F4/80 and Gr-1. *p

    Article Snippet: For IHC analysis, frozen sections were incubated with antibodies to human A20, and mouse iNOS (Santa Cruz Biotechnology Inc, Santa Cruz, CA), VCAM-1, CD4, CD8, B-220 and GR-1 (BD Biosciences, San Jose, CA), CD3 (AbD Serotec, Raleigh, NC), F4/80 (AbD Serotec, Raleigh, NC), CD25 and FoxP3 (Biolegend, San Diego, CA), followed by biotinylated secondary antibodies (Vector, Burlingame, CA) ( ).

    Techniques: Over Expression, Immunohistochemistry, Immunostaining, TUNEL Assay, Staining, Mouse Assay

    MBL modulates ALD-DNA–induced macrophage M2b polarization. ALD-DNA was pre-incubated with indicated mouse MBL for 2 h. RAW264.7 cells or peritoneal macrophages were cultured with PBS, ALD-DNA or ALD-DNA/MBL complexes for 24 h. (A and C) mRNA levels of TNF-α, MCP-1, IL-6 and IL-10 in RAW264.7 cells or peritoneal macrophages were analyzed by real-time PCR. (B and D) Protein levels of TNF-α, MCP-1, IL-6 and IL-10 in the supernatants of RAW264.7 cells or peritoneal macrophages were measured by ELISA. (E and F) RAW264.7 cells were cultured with PBS, ALD-DNA or ALD-DNA/MBL complexes for 24 h. Then cell lysates were prepared to measure the protein levels of iNOS by western blot analysis. All values are given relative to the expression level of the β-actin. Data are means ± SD of three independent experiments. * p

    Journal: PLoS ONE

    Article Title: Mannose-Binding Lectin Blunts Macrophage Polarization and Ameliorates Lupus Nephritis

    doi: 10.1371/journal.pone.0062465

    Figure Lengend Snippet: MBL modulates ALD-DNA–induced macrophage M2b polarization. ALD-DNA was pre-incubated with indicated mouse MBL for 2 h. RAW264.7 cells or peritoneal macrophages were cultured with PBS, ALD-DNA or ALD-DNA/MBL complexes for 24 h. (A and C) mRNA levels of TNF-α, MCP-1, IL-6 and IL-10 in RAW264.7 cells or peritoneal macrophages were analyzed by real-time PCR. (B and D) Protein levels of TNF-α, MCP-1, IL-6 and IL-10 in the supernatants of RAW264.7 cells or peritoneal macrophages were measured by ELISA. (E and F) RAW264.7 cells were cultured with PBS, ALD-DNA or ALD-DNA/MBL complexes for 24 h. Then cell lysates were prepared to measure the protein levels of iNOS by western blot analysis. All values are given relative to the expression level of the β-actin. Data are means ± SD of three independent experiments. * p

    Article Snippet: Abs used here were anti-mouse iNOS (Santa Cruz Biotechnology), anti-mouse MBL (Santa Cruz Biotechnology), anti-IκB (Cell Signaling Technology), anti–β-actin (Santa Cruz Biotechnology), anti-CREB (Cell Signaling Technology), anti–phospho-CREB (Cell Signaling Technology), anti-ERK1/2 (Cell Signaling Technology), anti-phospho-ERK1/2 (Cell Signaling Technology), anti-p38 (Cell Signaling Technology), anti-phospho-p38 (Cell Signaling Technology), anti-JNK (Cell Signaling Technology), anti-phospho-JNK (Cell Signaling Technology), anti-goat IgG-HRP (Santa Cruz Biotechnology), anti-mouse IgG-HRP (Santa Cruz Biotechnology), and goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology).

    Techniques: Incubation, Cell Culture, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing

    Inhibition of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression by Zuonin B. RAW264.7 cells were pretreated with different concentrations of Zuonin B for 1 h and stimulated with LPS (1 μ g/mL) for a further 24 h. Equal amounts of protein in cell lysates were electrophoresed, and the protein expression levels of iNOS (a) and COX-2 (b) determined using specific antibodies for iNOS and COX-2. The respective levels of β -actin were used to confirm equal amounts of protein loading for electrophoresis. Interleukin COX-2 and iNOS mRNA levels in lung tissue, (c) Cells were cultured for 24 h with LPS (1 μ g/mL), fixed, permeabilized, and incubated with rabbit polyclonal anti-iNOS and COX-2 antibody, followed by Texas-red conjugated anti-rabbit Ig (red). The nuclei of the corresponding cells were visualized with 4′,6-diamidino-2-phenylindole (DAPI) (magnification ×400). NC: untreated control cells; LPS: LPS only treatment.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Zuonin B Inhibits Lipopolysaccharide-Induced Inflammation via Downregulation of the ERK1/2 and JNK Pathways in RAW264.7 Macrophages

    doi: 10.1155/2012/728196

    Figure Lengend Snippet: Inhibition of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression by Zuonin B. RAW264.7 cells were pretreated with different concentrations of Zuonin B for 1 h and stimulated with LPS (1 μ g/mL) for a further 24 h. Equal amounts of protein in cell lysates were electrophoresed, and the protein expression levels of iNOS (a) and COX-2 (b) determined using specific antibodies for iNOS and COX-2. The respective levels of β -actin were used to confirm equal amounts of protein loading for electrophoresis. Interleukin COX-2 and iNOS mRNA levels in lung tissue, (c) Cells were cultured for 24 h with LPS (1 μ g/mL), fixed, permeabilized, and incubated with rabbit polyclonal anti-iNOS and COX-2 antibody, followed by Texas-red conjugated anti-rabbit Ig (red). The nuclei of the corresponding cells were visualized with 4′,6-diamidino-2-phenylindole (DAPI) (magnification ×400). NC: untreated control cells; LPS: LPS only treatment.

    Article Snippet: After washing with PBS and blocking with 3% bovine serum albumin in PBS for 30 min, samples were incubated overnight at 4°C with rabbit polyclonal anti-iNOS, anti-COX-2 (1 : 500 dilution, Santa Cruz Biotechnology, Santa Cruz, CA, USA), and anti-NF-κ B p65 subunit (1 : 500 dilution, Assay Designs) antibodies.

    Techniques: Inhibition, Expressing, Electrophoresis, Cell Culture, Incubation

    mGSTA4-4 Expression and iNOS upregulation in mGSTA4 -transfected MS1 cells. Panel A: mGSTA4-4 expression by Western blot analysis. 50 μg cell lysates were loaded on 4–12% SDS-PAGE. The primary polyclonal rabbit-anti-mGSTA4-4 antibodies

    Journal: Toxicology and applied pharmacology

    Article Title: Endothelial glutathione-S-transferase A4-4 protects against oxidative stress and modulates iNOS expression through NF-?B translocation

    doi: 10.1016/j.taap.2008.03.018

    Figure Lengend Snippet: mGSTA4-4 Expression and iNOS upregulation in mGSTA4 -transfected MS1 cells. Panel A: mGSTA4-4 expression by Western blot analysis. 50 μg cell lysates were loaded on 4–12% SDS-PAGE. The primary polyclonal rabbit-anti-mGSTA4-4 antibodies

    Article Snippet: Lesions and adjacent sections of selected lesions were immunostained for 4-HNE and iNOS with goat anti-HNE antiserum (Alpha Diagnostic, San Antonio, TX), and rabbit anti-iNOS polyclonal antibodies (Santa Cruz Biotechnology., Santa Cruz, CA).

    Techniques: Expressing, Transfection, Western Blot, SDS Page

    Participation of PPARγ in iNOS inhibition by HP 24 . (A) Peritoneal macrophages from T. cruzi -infected mice (9 dpi) were obtained. Cells were transfected with PPARγ-siRNA during 72 h. Transfected or non-transfected cells were treated with HP 24 (100 μM) for 48 h. iNOS expression was detected by immunofluorescence with a rabbit polyclonal anti-iNOS antibody and with a secondary goat anti-rabbit Alexa 488-labeled antibody. Cell nuclei were stained with DAPI. Mean fluorescence intensity (MFI) represents iNOS expression. Representative microphotographs are shown. Scale bar: 10 μm. (B) Western blot analysis was carried out and iNOS expression was determined. Protein levels were normalized against α-actin. (C) NO release to culture supernatants was analyzed by the Griess method. Results are expressed as the mean of three independent experiments (six mice/group). * P

    Journal: Frontiers in Immunology

    Article Title: Pyridinecarboxylic Acid Derivative Stimulates Pro-Angiogenic Mediators by PI3K/AKT/mTOR and Inhibits Reactive Nitrogen and Oxygen Species and NF-κB Activation Through a PPARγ-Dependent Pathway in T. cruzi-Infected Macrophages

    doi: 10.3389/fimmu.2019.02955

    Figure Lengend Snippet: Participation of PPARγ in iNOS inhibition by HP 24 . (A) Peritoneal macrophages from T. cruzi -infected mice (9 dpi) were obtained. Cells were transfected with PPARγ-siRNA during 72 h. Transfected or non-transfected cells were treated with HP 24 (100 μM) for 48 h. iNOS expression was detected by immunofluorescence with a rabbit polyclonal anti-iNOS antibody and with a secondary goat anti-rabbit Alexa 488-labeled antibody. Cell nuclei were stained with DAPI. Mean fluorescence intensity (MFI) represents iNOS expression. Representative microphotographs are shown. Scale bar: 10 μm. (B) Western blot analysis was carried out and iNOS expression was determined. Protein levels were normalized against α-actin. (C) NO release to culture supernatants was analyzed by the Griess method. Results are expressed as the mean of three independent experiments (six mice/group). * P

    Article Snippet: For these purposes, rabbit polyclonal IgG anti-iNOS (Santa Cruz Biotechnology, CA, USA; Cat#sc-650), rabbit polyclonal IgG anti-p65 (Santa Cruz Biotechnology, CA, USA; Cat#sc-109), and a rabbit polyclonal IgG directed to T. cruzi developed in our laboratory were used as primary antibodies at a 1:50 dilution, and goat anti-rabbit IgG Alexa Fluor 488 nm (for iNOS) (Jackson ImmunoResearch Labs; Cat# 111-545-003) or goat anti-rabbit IgG Alexa Fluor 647 nm (for p65 and T. cruzi ) (Jackson ImmunoResearch Labs; Cat# 111-605-003) was used at a 1:500 dilution as secondary antibodies.

    Techniques: Inhibition, Infection, Mouse Assay, Transfection, Expressing, Immunofluorescence, Labeling, Staining, Fluorescence, Western Blot

    Inhibiting TNFα and iNOS production by human marrow stromal cell treatment in SOD1 transgenic mice. Lumbar spinal cord tissues from wild-type mice, PBS-treated and human marrow stromal cell (hMSC)-treated transgenic mice at the age of 11 weeks are assayed for inducible nitric oxide synthase (iNOS) protein expression by Immunoblot and TNFα level by ELISA. ( A ) Representative immunoblots for iNOS and β-actin of the lumbar spinal cord from PBS-treated transgenic mice (1), wild-type mice (2) and hMSC-treated transgenic mice (3). ( B ) Quantitative evaluation through optical densitometry of iNOS blot and relation to β-actin blot. The levels of iNOS in Cu/Zn superoxide dismutase 1 (SOD1) transgenic mice receiving hMSC transplantation were significantly reduced compared with those in vehicle-treated mice (* P

    Journal: Journal of Neuroinflammation

    Article Title: Human marrow stromal cells reduce microglial activation to protect motor neurons in a transgenic mouse model of amyotrophic lateral sclerosis

    doi: 10.1186/1742-2094-10-52

    Figure Lengend Snippet: Inhibiting TNFα and iNOS production by human marrow stromal cell treatment in SOD1 transgenic mice. Lumbar spinal cord tissues from wild-type mice, PBS-treated and human marrow stromal cell (hMSC)-treated transgenic mice at the age of 11 weeks are assayed for inducible nitric oxide synthase (iNOS) protein expression by Immunoblot and TNFα level by ELISA. ( A ) Representative immunoblots for iNOS and β-actin of the lumbar spinal cord from PBS-treated transgenic mice (1), wild-type mice (2) and hMSC-treated transgenic mice (3). ( B ) Quantitative evaluation through optical densitometry of iNOS blot and relation to β-actin blot. The levels of iNOS in Cu/Zn superoxide dismutase 1 (SOD1) transgenic mice receiving hMSC transplantation were significantly reduced compared with those in vehicle-treated mice (* P

    Article Snippet: The blots were blocked with 5% (w/v) defatted milk in TBS-Tween20 (pH 8.0) for 1 hour at room temperature and then incubated overnight at 4°C with primary antibodies: polyclonal rabbit anti-mouse inducible nitric oxide synthase (iNOS) (1:1,000; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and mouse anti-actin (1:1,000; Santa Cruz Biotechnology).

    Techniques: Transgenic Assay, Mouse Assay, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Transplantation Assay

    Th17-associated cytokines regulate the IFN-γ production and inducible nitric oxide synthase enzyme (iNOS) expression during the experiment of virulent yeast strain 18 of Paracoccidioides brasiliensis (Pb18) infection. (A,B) C57BL/6, IL-6 −/− , IL-23 −/− , and IL-17 receptor A (IL-17RA) −/− mice were infected (intravenously) with 1 × 10 6 Pb18 yeast cells. (A) IFN-γ levels were measured by enzyme-linked immunosorbent assay in the lung homogenate at 15 and 30 days postinfection (dpi). (B) Immunohistochemistry was performed in lung tissue to analyze the iNOS expression at 30 dpi. (C) The percentage of the pulmonary area containing iNOS + cells is indicated. Lung sections were stained for iNOS at 30 dpi using immunohistochemistry. Scale bar: 50 µm. Similar results were obtained in three independent experiments. Data represent the mean ± SEM of five mice. Uninfected mice are represented by the dashed line. * p

    Journal: Frontiers in Immunology

    Article Title: Th17-Inducing Cytokines IL-6 and IL-23 Are Crucial for Granuloma Formation during Experimental Paracoccidioidomycosis

    doi: 10.3389/fimmu.2017.00949

    Figure Lengend Snippet: Th17-associated cytokines regulate the IFN-γ production and inducible nitric oxide synthase enzyme (iNOS) expression during the experiment of virulent yeast strain 18 of Paracoccidioides brasiliensis (Pb18) infection. (A,B) C57BL/6, IL-6 −/− , IL-23 −/− , and IL-17 receptor A (IL-17RA) −/− mice were infected (intravenously) with 1 × 10 6 Pb18 yeast cells. (A) IFN-γ levels were measured by enzyme-linked immunosorbent assay in the lung homogenate at 15 and 30 days postinfection (dpi). (B) Immunohistochemistry was performed in lung tissue to analyze the iNOS expression at 30 dpi. (C) The percentage of the pulmonary area containing iNOS + cells is indicated. Lung sections were stained for iNOS at 30 dpi using immunohistochemistry. Scale bar: 50 µm. Similar results were obtained in three independent experiments. Data represent the mean ± SEM of five mice. Uninfected mice are represented by the dashed line. * p

    Article Snippet: Briefly, slides were incubated with anti-mouse IgG as control or rabbit IgG anti-mouse inducible nitric oxide synthase (iNOS) enzyme (Santa Cruz Biotechnology, Santa Cruz, CA, USA) diluted 100 times in PBS 0.01% saponin.

    Techniques: Expressing, Infection, Mouse Assay, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Staining

    Role of iNOS in neutrophil apoptosis. ( a ) Neutrophil apoptosis was measured by Annexin V-PI labelling in scrambled, iNOS and nNOS silenced human PMNs after 12 h of incubation ( N =5). ( b ) Caspase-3 activity assay was performed in scrambled, iNOS and nNOS silenced human PMNs ( N =3). ( c ) Annexin V-PI labelling of BMDN from WT, iNOS and nNOS KO mice after 18 h of in vitro culture ( N =5). ( d ) Caspase-3 activity was monitored in BMDN from WT, iNOS and nNOS KO mice using caspase-3 specific fluorogenic substrate acetyl-Asp-Glu-Val-Asp-7-amino-4 methylcoumarin (150 μ M; N =5). Mitochondrial membrane potential in control and iNOS silenced human PMNs ( e ) and WT and iNOS KO BMDN ( f ) ( N =5).Data represent mean±S.E.M. of three to five independent experiments. * P

    Journal: Cell Death & Disease

    Article Title: Nitric oxide-mediated apoptosis of neutrophils through caspase-8 and caspase-3-dependent mechanism

    doi: 10.1038/cddis.2016.248

    Figure Lengend Snippet: Role of iNOS in neutrophil apoptosis. ( a ) Neutrophil apoptosis was measured by Annexin V-PI labelling in scrambled, iNOS and nNOS silenced human PMNs after 12 h of incubation ( N =5). ( b ) Caspase-3 activity assay was performed in scrambled, iNOS and nNOS silenced human PMNs ( N =3). ( c ) Annexin V-PI labelling of BMDN from WT, iNOS and nNOS KO mice after 18 h of in vitro culture ( N =5). ( d ) Caspase-3 activity was monitored in BMDN from WT, iNOS and nNOS KO mice using caspase-3 specific fluorogenic substrate acetyl-Asp-Glu-Val-Asp-7-amino-4 methylcoumarin (150 μ M; N =5). Mitochondrial membrane potential in control and iNOS silenced human PMNs ( e ) and WT and iNOS KO BMDN ( f ) ( N =5).Data represent mean±S.E.M. of three to five independent experiments. * P

    Article Snippet: Scrambled, iNOS and nNOS siRNA, anti-iNOS-PE, anti-Bax-PE and CD15-PE antibodies were procured from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Techniques: Incubation, Caspase-3 Activity Assay, Mouse Assay, In Vitro, Activity Assay

    iNOS/NO production during human PMNs spontaneous apoptosis. ( a ) NO generation as measured by DAF. Control human PMNs (1 × 10 6 cells/ml) were incubated with DAF after 12 and 18 h ( N =6). ( b ) Total nitrite content measured using Griess reagent in fresh control and apoptotic cell after 12 and 18 h ( N =6). iNOS expression was monitored by real-time PCR ( c ) in fresh control and apoptotic human PMNs and western blotting ( d ) ( N =5). ( e ) Immunolabelling of iNOS in fresh and apoptotic neutrophils. Cells were fixed and permeabilized followed by labelling with iNOS and Bax ( N =2). Scale bar-5 μ m. Data represent mean±S.E.M. of three to five independent experiments. * P

    Journal: Cell Death & Disease

    Article Title: Nitric oxide-mediated apoptosis of neutrophils through caspase-8 and caspase-3-dependent mechanism

    doi: 10.1038/cddis.2016.248

    Figure Lengend Snippet: iNOS/NO production during human PMNs spontaneous apoptosis. ( a ) NO generation as measured by DAF. Control human PMNs (1 × 10 6 cells/ml) were incubated with DAF after 12 and 18 h ( N =6). ( b ) Total nitrite content measured using Griess reagent in fresh control and apoptotic cell after 12 and 18 h ( N =6). iNOS expression was monitored by real-time PCR ( c ) in fresh control and apoptotic human PMNs and western blotting ( d ) ( N =5). ( e ) Immunolabelling of iNOS in fresh and apoptotic neutrophils. Cells were fixed and permeabilized followed by labelling with iNOS and Bax ( N =2). Scale bar-5 μ m. Data represent mean±S.E.M. of three to five independent experiments. * P

    Article Snippet: Scrambled, iNOS and nNOS siRNA, anti-iNOS-PE, anti-Bax-PE and CD15-PE antibodies were procured from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Techniques: Incubation, Expressing, Real-time Polymerase Chain Reaction, Western Blot

    Downregulation of inflammation. (a1) Cells were pretreated with different concentrations of FFSO for 30 minutes and then stimulated with LPS (1 microg/mL) for 12 hours. Ratio of TNF- α to GAPDH expression in cells was determined by real-time PCR, as described in Section 2 . (a2) Cells were pretreated with the indicated concentrations of FFSO for 2 hours and then stimulated with LPS (1 microg/mL) for 1 hour, after which nuclear extracts were prepared. Detection of NF- κ B-binding activities was performed as described in Section 2 . This experiment was repeated two times with similar results. (a3) Ratio of COX-2 to GAPDH expression on cells same as (a1). (a4) Cells were pretreated with different concentrations of FFSO for 30 minutes and then stimulated with LPS (1 microg/mL) for another 24 hours. Expression of iNOS and COX-2 proteins was detected by Western blotting using specific anti-iNOS and anti-COX-2 antibodies. β -Actin protein was used as an internal control. Expression of TNF- α , NF-kB p65, iNOS, and COX-2 (arrow indicates dark brown) was induced by D. farinae in the AE group. These positive reactions in the AT group were remarkably decreased compared with those in the AE group (immunohistochemistry; Bar size, 50 μ m). Abbreviations are the same as in Figure 1 . ∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Therapeutic Effects of Fermented Flax Seed Oil on NC/Nga Mice with Atopic Dermatitis-Like Skin Lesions

    doi: 10.1155/2017/5469125

    Figure Lengend Snippet: Downregulation of inflammation. (a1) Cells were pretreated with different concentrations of FFSO for 30 minutes and then stimulated with LPS (1 microg/mL) for 12 hours. Ratio of TNF- α to GAPDH expression in cells was determined by real-time PCR, as described in Section 2 . (a2) Cells were pretreated with the indicated concentrations of FFSO for 2 hours and then stimulated with LPS (1 microg/mL) for 1 hour, after which nuclear extracts were prepared. Detection of NF- κ B-binding activities was performed as described in Section 2 . This experiment was repeated two times with similar results. (a3) Ratio of COX-2 to GAPDH expression on cells same as (a1). (a4) Cells were pretreated with different concentrations of FFSO for 30 minutes and then stimulated with LPS (1 microg/mL) for another 24 hours. Expression of iNOS and COX-2 proteins was detected by Western blotting using specific anti-iNOS and anti-COX-2 antibodies. β -Actin protein was used as an internal control. Expression of TNF- α , NF-kB p65, iNOS, and COX-2 (arrow indicates dark brown) was induced by D. farinae in the AE group. These positive reactions in the AT group were remarkably decreased compared with those in the AE group (immunohistochemistry; Bar size, 50 μ m). Abbreviations are the same as in Figure 1 . ∗ P

    Article Snippet: The proteolyzed slices were incubated in blocking serum (10% normal goat serum) for 4 hours, after which slices were incubated with the primary antibodies goat anti-p-ERK 1/2 (1 : 100, Santa Cruz Biotec, USA), goat anti-Fc ε receptor (1 : 100, Santa Cruz Biotec), goat anti-substance-P (1 : 100, Santa Cruz Biotec), goat anti-MMP-9 (1 : 100, Santa Cruz Biotec), goat anti-5HT (1 : 100, Santa Cruz Biotec), goat anti-TNF-α (1 : 250, Santa Cruz Biotec), goat anti-NF-κ B p65 (1 : 500, Santa Cruz Biotec), goat anti-iNOS (1 : 250, Santa Cruz Biotec), goat anti-COX-2 (1 : 100, Santa Cruz Biotec), goat anti-LXR (1 : 200, Santa Cruz Biotec), goat anti-PKC (1 : 100, Santa), goat anti-IL4 (1 : 100, Santa Cruz Biotec), goat anti-STAT 6 (1 : 100, Santa Cruz Biotec), goat anti-IL6 (1 : 100, Santa Cruz Biotec), and goat anti-STAT 3 (1 : 100, Santa Cruz Biotec) for 72 hours in a 4°C humidified chamber.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Binding Assay, Western Blot, Immunohistochemistry

    Effects of SSM on SNCI-induced protein expression of TNF-α, IL-6, iNOS, and COX-2 . The sciatic nerve was crushed for 30 s using a surgical clip. (a) Relative protein expression levels of TNF- α and IL-6. (b) Relative protein expression levels of iNOS and COX-2. Bands were detected using an enhanced chemiluminescence (ECL) detection kit. Actin was used as an internal control (46 kDa). (A) Sham operation group, (B) SNCI-induced group, (C) SNCI-induced and 0.1 g/kg SSM-treated group, (D) SNCI-induced and 1 g/kg SSM-treated group, and (E) SNCI-induced and 10 g/kg SSM-treated group. The results are presented as the means ± standard errors of the mean (SEMs). ∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Scolopendra subspinipes mutilans Extract Suppresses Inflammatory and Neuropathic Pain In Vitro and In Vivo

    doi: 10.1155/2018/5057372

    Figure Lengend Snippet: Effects of SSM on SNCI-induced protein expression of TNF-α, IL-6, iNOS, and COX-2 . The sciatic nerve was crushed for 30 s using a surgical clip. (a) Relative protein expression levels of TNF- α and IL-6. (b) Relative protein expression levels of iNOS and COX-2. Bands were detected using an enhanced chemiluminescence (ECL) detection kit. Actin was used as an internal control (46 kDa). (A) Sham operation group, (B) SNCI-induced group, (C) SNCI-induced and 0.1 g/kg SSM-treated group, (D) SNCI-induced and 1 g/kg SSM-treated group, and (E) SNCI-induced and 10 g/kg SSM-treated group. The results are presented as the means ± standard errors of the mean (SEMs). ∗ P

    Article Snippet: The membranes were immersed in blocking buffer for 1 h and reacted at 4°C overnight with primary antibody: mouse anti-β -actin, rabbit anti-iNOS, goat anti-COX-2, anti-TNF-α , and anti-IL-6 (1:1000; Santa Cruz Biotechnology).

    Techniques: Expressing, Cross-linking Immunoprecipitation

    Effects of SSM on LPS-induced protein expression of TNF-α, IL-6, iNOS, and COX-2 . RAW 264.7 cells were induced with 1 μ g/mL LPS and various concentrations of SSM for 24 h. (a) Relative protein expression levels of TNF- α and IL-6. (b) Relative protein expression levels of iNOS and COX-2. Bands were detected using an enhanced chemiluminescence (ECL) detection kit. Actin was used as an internal control (46 kDa). (A) Control group, (B) 1 μ g/mL LPS-administered group, (C) 1 μ g/mL LPS-administered and 0.1 μ g/mL SSM-treated group, (D) 1 μ g/mL LPS-administered and 1 μ g/mL SSM-treated group, and (E) 1 μ g/mL LPS-administered and 10 μ g/mL SSM-treated group. The results are presented as the means ± standard errors of the mean (SEMs). ∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Scolopendra subspinipes mutilans Extract Suppresses Inflammatory and Neuropathic Pain In Vitro and In Vivo

    doi: 10.1155/2018/5057372

    Figure Lengend Snippet: Effects of SSM on LPS-induced protein expression of TNF-α, IL-6, iNOS, and COX-2 . RAW 264.7 cells were induced with 1 μ g/mL LPS and various concentrations of SSM for 24 h. (a) Relative protein expression levels of TNF- α and IL-6. (b) Relative protein expression levels of iNOS and COX-2. Bands were detected using an enhanced chemiluminescence (ECL) detection kit. Actin was used as an internal control (46 kDa). (A) Control group, (B) 1 μ g/mL LPS-administered group, (C) 1 μ g/mL LPS-administered and 0.1 μ g/mL SSM-treated group, (D) 1 μ g/mL LPS-administered and 1 μ g/mL SSM-treated group, and (E) 1 μ g/mL LPS-administered and 10 μ g/mL SSM-treated group. The results are presented as the means ± standard errors of the mean (SEMs). ∗ P

    Article Snippet: The membranes were immersed in blocking buffer for 1 h and reacted at 4°C overnight with primary antibody: mouse anti-β -actin, rabbit anti-iNOS, goat anti-COX-2, anti-TNF-α , and anti-IL-6 (1:1000; Santa Cruz Biotechnology).

    Techniques: Expressing

    Gr1 + (Ly6C+) inflammatory monocytes in the lamina propria express iNOS, TNFα, and IL-12 but not dendritic cell markers

    Journal:

    Article Title: Gr1+ (Ly6C+) Inflammatory Monocytes are Required for Mucosal Resistance to the Pathogen Toxoplasma gondii

    doi: 10.1016/j.immuni.2008.05.019

    Figure Lengend Snippet: Gr1 + (Ly6C+) inflammatory monocytes in the lamina propria express iNOS, TNFα, and IL-12 but not dendritic cell markers

    Article Snippet: Thereafter, cells were incubated on ice for 30 min with fluorescently conjugated antibodies for cell surface markers: Cy5-conjugated anti-Gr1 mAb RB6-8C5 (eBioscience, San Diego, CA), PE or APC- conjugated anti-F4/80 mAb A3-1 (AbD serotec, Raleigh, NC), FITC-conjugated hamster anti-mouse p150/90 (CD11c) (eBioscience), PE-conjugated anti-iNOS (M-19, Santa Cruz), FITC-conjugated anti-Ly6C mAb AL-21 (BD bioscience), PE-conjugated anti-Ly6G mAb 1A8 (BD bioscience), or Cy5-conjugated anti-CD11b mAb Mac-1α (eBioscience).

    Techniques:

    cSCK-pa 100 mediated silencing of iNOS expression by siRNA. Anti-iNOS siRNA481 (100 nM) was transfected into mouse monocyte-macrophage RAW264.7 cells using PAEA 128 - b -PS 40 cSCK-pa 100 at different N/P ratios (for N/P=1, cSCK 0.62 μg/mL),

    Journal: Nucleic Acid Therapeutics

    Article Title: Efficient Protection and Transfection of Small Interfering RNA by Cationic Shell-Crosslinked Knedel-Like Nanoparticles

    doi: 10.1089/nat.2012.0390

    Figure Lengend Snippet: cSCK-pa 100 mediated silencing of iNOS expression by siRNA. Anti-iNOS siRNA481 (100 nM) was transfected into mouse monocyte-macrophage RAW264.7 cells using PAEA 128 - b -PS 40 cSCK-pa 100 at different N/P ratios (for N/P=1, cSCK 0.62 μg/mL),

    Article Snippet: The separated proteins were then transferred onto polyvinylidene difluoride membrane (Amersham Hybond™ -P, GE Healthcare). iNOS was detected by using monoclonal anti-iNOS antibody NOS2(C-11) at 1:200 (Santa Cruz Biotechnology, Inc.). β-actin was detected using mouse anti- β-actin monoclonal antibody at 1:20,000 (GenScript Corporation).

    Techniques: Expressing, Transfection

    iNOS mRNA silencing by cSCK•siRNA•GALA nanocomplex in RAW264.7 cells. (A) cytoxicity of PAEA 160 -b-PS 30 cSCK-pa 100 after 24 h in RAW cells that had been induced for 24 hours with LPS/γ-IFN as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium

    Journal: Nucleic Acid Therapeutics

    Article Title: Efficient Protection and Transfection of Small Interfering RNA by Cationic Shell-Crosslinked Knedel-Like Nanoparticles

    doi: 10.1089/nat.2012.0390

    Figure Lengend Snippet: iNOS mRNA silencing by cSCK•siRNA•GALA nanocomplex in RAW264.7 cells. (A) cytoxicity of PAEA 160 -b-PS 30 cSCK-pa 100 after 24 h in RAW cells that had been induced for 24 hours with LPS/γ-IFN as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium

    Article Snippet: The separated proteins were then transferred onto polyvinylidene difluoride membrane (Amersham Hybond™ -P, GE Healthcare). iNOS was detected by using monoclonal anti-iNOS antibody NOS2(C-11) at 1:200 (Santa Cruz Biotechnology, Inc.). β-actin was detected using mouse anti- β-actin monoclonal antibody at 1:20,000 (GenScript Corporation).

    Techniques:

    Endocytosis inhibition and tracking studies. (A) Inhibition experiments. iNOS-induced RAW 264.7 cells were incubated with cytochalasin D (Cyto-D) (average of 2–50 μM), chlorpromazine (CPZ) (20 μM) and methyl-beta-cyclodextrin,

    Journal: Nucleic Acid Therapeutics

    Article Title: Efficient Protection and Transfection of Small Interfering RNA by Cationic Shell-Crosslinked Knedel-Like Nanoparticles

    doi: 10.1089/nat.2012.0390

    Figure Lengend Snippet: Endocytosis inhibition and tracking studies. (A) Inhibition experiments. iNOS-induced RAW 264.7 cells were incubated with cytochalasin D (Cyto-D) (average of 2–50 μM), chlorpromazine (CPZ) (20 μM) and methyl-beta-cyclodextrin,

    Article Snippet: The separated proteins were then transferred onto polyvinylidene difluoride membrane (Amersham Hybond™ -P, GE Healthcare). iNOS was detected by using monoclonal anti-iNOS antibody NOS2(C-11) at 1:200 (Santa Cruz Biotechnology, Inc.). β-actin was detected using mouse anti- β-actin monoclonal antibody at 1:20,000 (GenScript Corporation).

    Techniques: Inhibition, Incubation