Journal: Molecular Medicine Reports

Article Title: LMTK3 knockdown retards cell growth and invasion and promotes apoptosis in thyroid cancer

doi: 10.3892/mmr.2017.6262

Figure Lengend Snippet: Serum level of LMTK3 measured by ELISA in TCP, BTD and HV. Markedly elevated serum LMTK3 levels were detected in TCP compared with BTD and HV (n=6). Data are expressed as the mean ± standard error of the mean. ***P<0.001 vs. HV; ### P<0.001 vs. BTD. LMTK3, lemur tyrosine kinase-3; TCP, thyroid cancer patients; BTD, benign tumor diseases; HV, healthy volunteers.

Article Snippet: Following washing, the specimens were incubated with peroxidase-labeled polymer conjugated to goat anti-human LMTK3 (dilution, 1:4,000; cat. no. A0201; Beyotime Institute of Biotechnology, Haimen, China) in Tris-HCl buffer at room temperature for 30 min.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Molecular Medicine Reports

Article Title: LMTK3 knockdown retards cell growth and invasion and promotes apoptosis in thyroid cancer

doi: 10.3892/mmr.2017.6262

Figure Lengend Snippet: LMTK3 protein levels and clinical features in thyroid cancer.

Article Snippet: Following washing, the specimens were incubated with peroxidase-labeled polymer conjugated to goat anti-human LMTK3 (dilution, 1:4,000; cat. no. A0201; Beyotime Institute of Biotechnology, Haimen, China) in Tris-HCl buffer at room temperature for 30 min.

Techniques:

Journal: Molecular Medicine Reports

Article Title: LMTK3 knockdown retards cell growth and invasion and promotes apoptosis in thyroid cancer

doi: 10.3892/mmr.2017.6262

Figure Lengend Snippet: LMTK3 level measured in TCP, BTD and HV tissue. (A) LMTK3 expression visualized by immunohistochemistry staining (original magnification, ×400; scale bar=100 µm). (B) The mRNA expression of LMTK3 was markedly elevated in TCP compared with BTD tissue samples. (C) Protein levels of LMTK3 were increased in TCP compared with BTD tissue samples. Data are expressed as the mean ± standard error of the mean (n=35). **P<0.01 vs. BTD group. LMTK3, lemur tyrosine kinase-3; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; TCP, thyroid cancer patients; BTD, benign tumor diseases; HV, healthy volunteers.

Article Snippet: Following washing, the specimens were incubated with peroxidase-labeled polymer conjugated to goat anti-human LMTK3 (dilution, 1:4,000; cat. no. A0201; Beyotime Institute of Biotechnology, Haimen, China) in Tris-HCl buffer at room temperature for 30 min.

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Molecular Medicine Reports

Article Title: LMTK3 knockdown retards cell growth and invasion and promotes apoptosis in thyroid cancer

doi: 10.3892/mmr.2017.6262

Figure Lengend Snippet: LMTK3 knockdown retarded cell cycle and inhibited proliferation of SW579 cells. (A) The mRNA level of LMTK3 was reduced in the siRNA LMTK3 treated group compared with NC (n=6). (B) Protein expression of LMTK3 was significantly inhibited by treatment with LMTK3 siRNA compared with NC (n=3). (C) The effect of LMTK3 knockdown on cell cycle distribution in SW579 cells. Histograms illustrate SW579 cells treated with LMTK3 siRNA or exposed to NC, respectively (n=6). (D) Cell proliferation assessed by using an MTT assay. The result demonstrated that cell proliferation was decreased by treatment with LMTK3 siRNA, n=6. Data are expressed as the mean ± standard error of the mean. **P<0.01 vs. NC. LMTK3, lemur tyrosine kinase-3; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; siRNA, small interfering RNA; NC, negative control.

Article Snippet: Following washing, the specimens were incubated with peroxidase-labeled polymer conjugated to goat anti-human LMTK3 (dilution, 1:4,000; cat. no. A0201; Beyotime Institute of Biotechnology, Haimen, China) in Tris-HCl buffer at room temperature for 30 min.

Techniques: Expressing, MTT Assay, Small Interfering RNA, Negative Control

Journal: Molecular Medicine Reports

Article Title: LMTK3 knockdown retards cell growth and invasion and promotes apoptosis in thyroid cancer

doi: 10.3892/mmr.2017.6262

Figure Lengend Snippet: LMTK3 siRNA inhibits the migration and invasion of SW579 cells. (A) Migration/invasive cells were imaged following staining with hematoxylin (original magnification, ×200). (B) Histogram of the number of migrated cells. (C) Histogram of the number of invaded cells. Data are expressed as the mean ± standard error of the mean (n=6); **P<0.01 vs. NC. Ctrl, control group; NC, negative control; siRNA, small interfering RNA; LMTK3, lemur tyrosine kinase-3.

Article Snippet: Following washing, the specimens were incubated with peroxidase-labeled polymer conjugated to goat anti-human LMTK3 (dilution, 1:4,000; cat. no. A0201; Beyotime Institute of Biotechnology, Haimen, China) in Tris-HCl buffer at room temperature for 30 min.

Techniques: Migration, Staining, Negative Control, Small Interfering RNA

Journal: Molecular Medicine Reports

Article Title: LMTK3 knockdown retards cell growth and invasion and promotes apoptosis in thyroid cancer

doi: 10.3892/mmr.2017.6262

Figure Lengend Snippet: LMTK3 knockdown promoted apoptosis in SW579 cells. (A) Apoptotic cells were determined by TUNEL staining and visualized (magnification, ×200). Green=apoptotic cells; blue=cell nucleus stained by DAPI. Representative images are presented. (B) Calculation of apoptotic cells. The results indicate that LMTK3 knockdown induced apoptosis in SW579 cells (n=6 independent experiments). Data are expressed as the mean ± standard error of the mean. **P<0.01 vs. NC. Ctrl, control group; NC, negative control; siRNA, small interfering RNA; LMTK3, lemur tyrosine kinase-3; DAPI, 4′,6-diamidino-2-phenylindole; TUNEL, terminal dUTP nick end labeling.

Article Snippet: Following washing, the specimens were incubated with peroxidase-labeled polymer conjugated to goat anti-human LMTK3 (dilution, 1:4,000; cat. no. A0201; Beyotime Institute of Biotechnology, Haimen, China) in Tris-HCl buffer at room temperature for 30 min.

Techniques: TUNEL Assay, Staining, Negative Control, Small Interfering RNA, End Labeling