Journal: Antimicrobial Agents and Chemotherapy
Article Title: Kallistatin Ameliorates Influenza Virus Pathogenesis by Inhibition of Kallikrein-Related Peptidase 1-Mediated Cleavage of Viral Hemagglutinin
Figure Lengend Snippet: KLK1 expression is increased, whereas kallistatin expression is reduced, during acute influenza virus infection. (A to C) C57BL/6 mice were intranasally inoculated with 10 7 PFU of influenza A/WSN/33 (H1N1) virus or saline, and lung tissues were collected
Article Snippet: The primary antibodies used for immunoblotting included mouse monoclonal anti-prekallikrein/kallikrein antibody (Abcam), mouse anti-human kallistatin monoclonal antibody (ascitic fluid) , goat anti-influenza A virus (H1N1) antiserum (Virostat, Portland, ME, USA), mouse anti-influenza A H1N1 virus HA monoclonal antibody (C-102; Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-influenza A virus H2N2 HA antibody (Sino Biological Inc.), rabbit anti-influenza A virus H3N2 HA antibody (Sino Biological Inc.), and mouse monoclonal anti-β-actin peroxidase antibody (Sigma-Aldrich, St. Louis, MO, USA).
Techniques: Expressing, Infection, Mouse Assay