anti h2a 07 146 antibodies Merck Kgaa Search Results


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  • 81
    Merck KGaA anti monoubiquityl h2a
    Anti Monoubiquityl H2a, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 81/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti monoubiquityl h2a/product/Merck KGaA
    Average 81 stars, based on 5 article reviews
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    anti monoubiquityl h2a - by Bioz Stars, 2020-01
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    90
    Merck KGaA anti h2a
    Anti H2a, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti h2a/product/Merck KGaA
    Average 90 stars, based on 13 article reviews
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    93
    Merck KGaA anti γh2ax
    Absence of Rad51 foci in fibroblasts from patients II-4 II-5. Following exposure to either 3Gy IR or 50ngml -1 mitomycin C no Rad51 foci were detected in fibroblasts from II-4 and II-5. <t>γH2AX</t> foci indicate the presence of DNA DSB. The residual foci at 24h following IR are consistent with a DNA repair deficiency.
    Anti γh2ax, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Merck KGaA anti ubiquitin lys48 specific
    Absence of Rad51 foci in fibroblasts from patients II-4 II-5. Following exposure to either 3Gy IR or 50ngml -1 mitomycin C no Rad51 foci were detected in fibroblasts from II-4 and II-5. <t>γH2AX</t> foci indicate the presence of DNA DSB. The residual foci at 24h following IR are consistent with a DNA repair deficiency.
    Anti Ubiquitin Lys48 Specific, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 81/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ubiquitin lys48 specific/product/Merck KGaA
    Average 81 stars, based on 10 article reviews
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    anti ubiquitin lys48 specific - by Bioz Stars, 2020-01
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    89
    Merck KGaA atf4
    CuET inhibits the p97 pathway and induces cellular unfolded protein response (UPR) a ) Proteasome inhibitor MG132-treated cells (5μM, 6h) accumulate both forms of NRF1 (120- and 110-KDa bands, upper and lower arrows, respectively) while CuET-treated cells (1μM, 6h) accumulate only the non-cleaved 120-KDa form; b ) Inhibition of the NRF1 cleavage process (appearance of the lower band) by CuET and NMS873 (a p97 inhibitor; 5μM) in mouse NIH3T3 cells co-treated with the proteasome inhibitor MG132 (5μM for 6h); c ) Time-course example images from a FRAP experiment the quantitative analysis of which is shown in Fig. 2g (U-2OS cells, blue boxes mark areas before bleaching, arrows after bleaching); d ) U-2OS cells pre-extracted by TritonX and stained for K-48-polyUb. The Ab signal intensities for cells treated with DMSO, BTZ (1μM), NMS873 (10μM) and CuET (1μM) are analysed by microscopy-based cytometry and plotted below; e ) Western blot analysis of accumulated poly-Ub proteins in ultracentrifugation-separated microsomal fraction from U-2OS cells treated by mock, CuET (1μM), NMS873 (10μM) or BTZ (1μM) for 3h; f ) UPR in U-2OS and MDA-MB-231 cell lines induced by 6-h treatment with CuET (various concentrations) or positive controls (NMS873 5μM, tunicamycin 2μg/ml, thapsigargin 1μM) manifested by increased levels of Xbp1s, <t>ATF4</t> and p-eIF2a. Panels a-f are representative of two independent experiments.
    Atf4, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 89/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atf4/product/Merck KGaA
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    Image Search Results


    Absence of Rad51 foci in fibroblasts from patients II-4 II-5. Following exposure to either 3Gy IR or 50ngml -1 mitomycin C no Rad51 foci were detected in fibroblasts from II-4 and II-5. γH2AX foci indicate the presence of DNA DSB. The residual foci at 24h following IR are consistent with a DNA repair deficiency.

    Journal: PLoS Genetics

    Article Title: A Hypomorphic PALB2 Allele Gives Rise to an Unusual Form of FA-N Associated with Lymphoid Tumour Development

    doi: 10.1371/journal.pgen.1005945

    Figure Lengend Snippet: Absence of Rad51 foci in fibroblasts from patients II-4 II-5. Following exposure to either 3Gy IR or 50ngml -1 mitomycin C no Rad51 foci were detected in fibroblasts from II-4 and II-5. γH2AX foci indicate the presence of DNA DSB. The residual foci at 24h following IR are consistent with a DNA repair deficiency.

    Article Snippet: Antibodies used for immunoblotting were: ATM 11G12 (made in house), anti-phospho ATM (S1981) (AF1655; R & D Systems), anti-SMC1 (A300-055A; Bethyl Laboratories), anti-phospho SMC1 (S966) (A300-050A; Bethyl), anti-KAP-1 (A300-274A; Bethyl), anti-phospho KAP-1 (S824) (A300-767A; Bethyl), anti-NBN (ab23996; Abcam), anti-phospho NBN (S343) (ab47272; Abcam), anti-phospho CHK2 (T68) (2661; Cell Signaling Technology, New England Biolabs), anti-CREB (48H2; Cell Signaling), anti-phospho CREB (S121) (NB100-410; Novus Biologicals), anti-γH2AX (05–636; Merck Millipore), anti-H2A (07–146; Merck Millipore), anti-phospho BRCA1 (S1423) (Bethyl), anti-BRCA1 (OP92; Calbiochem), anti-Chk2 (gift from Dr S. Elledge), anti-53BP1 (NB100-904; Novus Biologicals) and anti-phospho 53BP1 (S1778) (2675; Cell Signaling Technology).

    Techniques:

    CuET inhibits the p97 pathway and induces cellular unfolded protein response (UPR) a ) Proteasome inhibitor MG132-treated cells (5μM, 6h) accumulate both forms of NRF1 (120- and 110-KDa bands, upper and lower arrows, respectively) while CuET-treated cells (1μM, 6h) accumulate only the non-cleaved 120-KDa form; b ) Inhibition of the NRF1 cleavage process (appearance of the lower band) by CuET and NMS873 (a p97 inhibitor; 5μM) in mouse NIH3T3 cells co-treated with the proteasome inhibitor MG132 (5μM for 6h); c ) Time-course example images from a FRAP experiment the quantitative analysis of which is shown in Fig. 2g (U-2OS cells, blue boxes mark areas before bleaching, arrows after bleaching); d ) U-2OS cells pre-extracted by TritonX and stained for K-48-polyUb. The Ab signal intensities for cells treated with DMSO, BTZ (1μM), NMS873 (10μM) and CuET (1μM) are analysed by microscopy-based cytometry and plotted below; e ) Western blot analysis of accumulated poly-Ub proteins in ultracentrifugation-separated microsomal fraction from U-2OS cells treated by mock, CuET (1μM), NMS873 (10μM) or BTZ (1μM) for 3h; f ) UPR in U-2OS and MDA-MB-231 cell lines induced by 6-h treatment with CuET (various concentrations) or positive controls (NMS873 5μM, tunicamycin 2μg/ml, thapsigargin 1μM) manifested by increased levels of Xbp1s, ATF4 and p-eIF2a. Panels a-f are representative of two independent experiments.

    Journal: Nature

    Article Title: Alcohol-abuse drug disulfiram targets cancer via p97 segregase adapter NPL4

    doi: 10.1038/nature25016

    Figure Lengend Snippet: CuET inhibits the p97 pathway and induces cellular unfolded protein response (UPR) a ) Proteasome inhibitor MG132-treated cells (5μM, 6h) accumulate both forms of NRF1 (120- and 110-KDa bands, upper and lower arrows, respectively) while CuET-treated cells (1μM, 6h) accumulate only the non-cleaved 120-KDa form; b ) Inhibition of the NRF1 cleavage process (appearance of the lower band) by CuET and NMS873 (a p97 inhibitor; 5μM) in mouse NIH3T3 cells co-treated with the proteasome inhibitor MG132 (5μM for 6h); c ) Time-course example images from a FRAP experiment the quantitative analysis of which is shown in Fig. 2g (U-2OS cells, blue boxes mark areas before bleaching, arrows after bleaching); d ) U-2OS cells pre-extracted by TritonX and stained for K-48-polyUb. The Ab signal intensities for cells treated with DMSO, BTZ (1μM), NMS873 (10μM) and CuET (1μM) are analysed by microscopy-based cytometry and plotted below; e ) Western blot analysis of accumulated poly-Ub proteins in ultracentrifugation-separated microsomal fraction from U-2OS cells treated by mock, CuET (1μM), NMS873 (10μM) or BTZ (1μM) for 3h; f ) UPR in U-2OS and MDA-MB-231 cell lines induced by 6-h treatment with CuET (various concentrations) or positive controls (NMS873 5μM, tunicamycin 2μg/ml, thapsigargin 1μM) manifested by increased levels of Xbp1s, ATF4 and p-eIF2a. Panels a-f are representative of two independent experiments.

    Article Snippet: :3933), anti-H2A, acidic patch (1:1000; Merck Millipore, cat. n.: 07-146), anti-monoubiquityl-H2A (1:1000; Merck Millipore, clone E6C5), anti-IκBα (1:500; Santa Cruz Biotechnology, cat. n.: sc-371), anti-p53 (1:500; Santa Cruz Biotechnology, clone DO-1), anti-HIF1α (1:1000; BD Biosciences, cat. n.: 610958), anti-Cdc25A (1:500; Santa Cruz Biotechnology, clone DCS-120), anti-NRF1 (1:1000, Cell Signaling, clone D5B10), anti-VCP (1:2000; Abcam, cat. n.: ab11433), anti-VCP (1:1000; Novus Bio, cat. n.: NBP100-1557), anti-NPLOC4 (1:1000; Novus Bio, cat. n.: NBP1-82166), anti-ubiquitin lys48-specific (1:1000; Merck Millipore, clone Apu2), anti-β-actin (1:2000; Santa Cruz Biotechnology, cat. n.: sc-1616; or 1:500, Santa Cruz Biotechnology, cat. n. sc-87778), anti-GAPDH (1:1000,GeneTex, clone 1D4), anti-Lamin B (1:1000; Santa Cruz Biotechnology, cat. n.: sc-6217), anti-calnexin (1:500; Santa Cruz Biotechnology, cat. n.: sc-11397), anti-α-Tubulin (1:500; Santa Cruz Biotechnology, cat. n.: sc-5286), anti-Xbp1 (1:500; Santa Cruz Biotechnology, cat. n.: sc-7160), Ufd1 (1:500; Abcam, cat. n.: ab155003), cleaved PARP1 (1:500; Cell Signaling, cat. n.: 9544), p-eIF2a (1:500; Cell Signaling, cat. n.: 3597), ATF4 (1:500; Merck Millipore, cat. n.: ABE387), HSP90 (1.500; Enzo, cat. n.: ADI-SPA-810), HSP70 (1:500; Enzo, cat. n.: ADI-SPA-830), HSF1(1:500; Cell Signaling, cat. n.: 4356), pHSP27 (1:1000; Abcam, cat. n.: 155987), HSP27 (1:1000; Abcam, cat. n.: 109376) followed by detection by secondary antibodies: goat-anti mouse IgG-HRP (GE Healthcare), goat-anti rabbit (GE Healthcare), donkey-anti goat IgG-HRP (Santa Cruz Biotechnology, sc-2020).

    Techniques: Inhibition, Staining, Microscopy, Cytometry, Western Blot, Multiple Displacement Amplification