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  • 95
    Millipore ctcf
    <t>CTCF</t> regulates <t>HPIP</t> expression in K562 cells. A, K562 cells were either treated or untreated with Epo (5 units/ml) for 4 h, and ChIP assay was performed using anti-CTCF antibody. IgG was used as control. CTCF occupancy over human HPIP promoter regions
    Ctcf, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ctcf/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ctcf - by Bioz Stars, 2021-09
    95/100 stars
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    96
    Abcam anti ctcf
    SH3PXD2A-AS1 recruits <t>CTCF</t> to the promoters of SH3PXD2A and <t>CCR7</t> and inhibits their transcription. a The expression level of CTCF in HTR8/SVneo cells transfected with full-length CTCF. Left: results of qPCR; right: results of western blot. b The expression level of CTCF in HTR8/SVneo cells transfected with shRNA of CTCF. Left: results of qPCR; right: results of western blot. c The reduced expression of SH3PXD2A and CCR7 in HTR8/SVneo cells with CTCF overexpression. Left: results of qPCR; right: results of western blot. d The increased expression of SH3PXD2A and CCR7 in HTR8/SVneo cells with shRNA of CTCF. Left: results of qPCR; right: results of western blot. e CTCF’s binding to the promoters of SH3PXD2A and CCR7 detected by ChIP assays and qPCR in HTR8/SVneo cells. f CTCF’s binding to the promoter of SH3PXD2A-2 detected by ChIP assays with SH3PXD2A-AS1 overexpression or knockout in HTR8/SVneo cells. g CTCF’s binding to the promoter of CCR7-3 detected in HTR8/SVneo cells with SH3PXD2A-AS1 overexpression or knockout using ChIP assays. h The transcription activity of SH3PXD2A-2 and CCR7-3 promoters detected in HTR8/SVneo cells with SH3PXD2A-AS1 overexpression or knockout using luciferase reporter assay. ** p
    Anti Ctcf, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ctcf/product/Abcam
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ctcf - by Bioz Stars, 2021-09
    96/100 stars
      Buy from Supplier

    Image Search Results


    CTCF regulates HPIP expression in K562 cells. A, K562 cells were either treated or untreated with Epo (5 units/ml) for 4 h, and ChIP assay was performed using anti-CTCF antibody. IgG was used as control. CTCF occupancy over human HPIP promoter regions

    Journal: The Journal of Biological Chemistry

    Article Title: Functional Regulation of Pre-B-cell Leukemia Homeobox Interacting Protein 1 (PBXIP1/HPIP) in Erythroid Differentiation *

    doi: 10.1074/jbc.M111.289843

    Figure Lengend Snippet: CTCF regulates HPIP expression in K562 cells. A, K562 cells were either treated or untreated with Epo (5 units/ml) for 4 h, and ChIP assay was performed using anti-CTCF antibody. IgG was used as control. CTCF occupancy over human HPIP promoter regions

    Article Snippet: Western blotting was performed using antibodies against HPIP from Bethyl Laboratories; GAPDH, acetyl-H3K4, and CTCF from Millipore; GATA1, phospho-AKT Ser-473, phosphor-GSK3β, total AKT, and total GSK3β from Cell Signaling Technologies; C/EBPα from Santa Cruz Biotechnology and Alexis; and FLAG from Sigma.

    Techniques: Expressing, Chromatin Immunoprecipitation

    BDNF protein expression in Neuro-2a (N2a)-neuroblastoma cells treated with (A) 10, 20 and 40 μM of DHA for 24 h and (B) CTCF-siRNA followed by 40 μM of DHA treatment. (C) CTCF protein expression in N2a cells treated with CTCF-siRNA followed

    Journal: Neurobiology of disease

    Article Title: Interactive actions of Bdnf methylation and cell metabolism for building neural resilience under the influence of diet

    doi: 10.1016/j.nbd.2014.09.014

    Figure Lengend Snippet: BDNF protein expression in Neuro-2a (N2a)-neuroblastoma cells treated with (A) 10, 20 and 40 μM of DHA for 24 h and (B) CTCF-siRNA followed by 40 μM of DHA treatment. (C) CTCF protein expression in N2a cells treated with CTCF-siRNA followed

    Article Snippet: Anti-BDNF (1:300; Santa Cruz Biotechnology, CA, USA) and anti-CTCF (1:300, Millipore, MA, USA) antibodies, diluted in 1% bovine serum albumin (BSA)/TBST were added to the cells and incubated overnight at 4°C.

    Techniques: Expressing

    (A) CTCF Binding at promoter region of Bdnf exon IV in low omega-3 (Low-n-3) or high omega-3 diet (High-n-3) fed animals, which were either continued to the same diet i.e. no transition to western diet (Low-n-3/No WD and High-n-3/No WD) or switched to

    Journal: Neurobiology of disease

    Article Title: Interactive actions of Bdnf methylation and cell metabolism for building neural resilience under the influence of diet

    doi: 10.1016/j.nbd.2014.09.014

    Figure Lengend Snippet: (A) CTCF Binding at promoter region of Bdnf exon IV in low omega-3 (Low-n-3) or high omega-3 diet (High-n-3) fed animals, which were either continued to the same diet i.e. no transition to western diet (Low-n-3/No WD and High-n-3/No WD) or switched to

    Article Snippet: Anti-BDNF (1:300; Santa Cruz Biotechnology, CA, USA) and anti-CTCF (1:300, Millipore, MA, USA) antibodies, diluted in 1% bovine serum albumin (BSA)/TBST were added to the cells and incubated overnight at 4°C.

    Techniques: Binding Assay, Western Blot

    SH3PXD2A-AS1 recruits CTCF to the promoters of SH3PXD2A and CCR7 and inhibits their transcription. a The expression level of CTCF in HTR8/SVneo cells transfected with full-length CTCF. Left: results of qPCR; right: results of western blot. b The expression level of CTCF in HTR8/SVneo cells transfected with shRNA of CTCF. Left: results of qPCR; right: results of western blot. c The reduced expression of SH3PXD2A and CCR7 in HTR8/SVneo cells with CTCF overexpression. Left: results of qPCR; right: results of western blot. d The increased expression of SH3PXD2A and CCR7 in HTR8/SVneo cells with shRNA of CTCF. Left: results of qPCR; right: results of western blot. e CTCF’s binding to the promoters of SH3PXD2A and CCR7 detected by ChIP assays and qPCR in HTR8/SVneo cells. f CTCF’s binding to the promoter of SH3PXD2A-2 detected by ChIP assays with SH3PXD2A-AS1 overexpression or knockout in HTR8/SVneo cells. g CTCF’s binding to the promoter of CCR7-3 detected in HTR8/SVneo cells with SH3PXD2A-AS1 overexpression or knockout using ChIP assays. h The transcription activity of SH3PXD2A-2 and CCR7-3 promoters detected in HTR8/SVneo cells with SH3PXD2A-AS1 overexpression or knockout using luciferase reporter assay. ** p

    Journal: Cell Death & Disease

    Article Title: Association of lncRNA SH3PXD2A-AS1 with preeclampsia and its function in invasion and migration of placental trophoblast cells

    doi: 10.1038/s41419-020-02796-0

    Figure Lengend Snippet: SH3PXD2A-AS1 recruits CTCF to the promoters of SH3PXD2A and CCR7 and inhibits their transcription. a The expression level of CTCF in HTR8/SVneo cells transfected with full-length CTCF. Left: results of qPCR; right: results of western blot. b The expression level of CTCF in HTR8/SVneo cells transfected with shRNA of CTCF. Left: results of qPCR; right: results of western blot. c The reduced expression of SH3PXD2A and CCR7 in HTR8/SVneo cells with CTCF overexpression. Left: results of qPCR; right: results of western blot. d The increased expression of SH3PXD2A and CCR7 in HTR8/SVneo cells with shRNA of CTCF. Left: results of qPCR; right: results of western blot. e CTCF’s binding to the promoters of SH3PXD2A and CCR7 detected by ChIP assays and qPCR in HTR8/SVneo cells. f CTCF’s binding to the promoter of SH3PXD2A-2 detected by ChIP assays with SH3PXD2A-AS1 overexpression or knockout in HTR8/SVneo cells. g CTCF’s binding to the promoter of CCR7-3 detected in HTR8/SVneo cells with SH3PXD2A-AS1 overexpression or knockout using ChIP assays. h The transcription activity of SH3PXD2A-2 and CCR7-3 promoters detected in HTR8/SVneo cells with SH3PXD2A-AS1 overexpression or knockout using luciferase reporter assay. ** p

    Article Snippet: The following primary antibodies were used: anti-Fish, anti-CCR7, anti-CTCF (Abcam, UK), anti-MMP2, anti-MMP3, anti-Vimentin, anti-N-cadherin (Cell Signaling Technology, USA), and anti-β-Actin (Cell Signaling Technology, USA).

    Techniques: Expressing, Transfection, Real-time Polymerase Chain Reaction, Western Blot, shRNA, Over Expression, Binding Assay, Chromatin Immunoprecipitation, Knock-Out, Activity Assay, Luciferase, Reporter Assay