Journal: Pathogens

Article Title: Epitope-Specific Response of Human Milk Immunoglobulins in COVID-19 Recovered Women

doi: 10.3390/pathogens10060705

Figure Lengend Snippet: Results of ELISA testing of human milk and blood serum samples of women that recovered from COVID-19 during pregnancy. ( A ) Distribution of the relative antibody levels calculated for IgA ( A , B ) and IgG ( C ) specific to SARS-CoV-2 S-protein NTD, RBD-SD1, and RBD (CHO) fragments, and N-protein in human milk ( A ) and blood serum ( B , C ). The dashed lines represent the cut-off value, samples with a relative antibody level ≥1 were considered positive. The statistical differences in antigen-specific relative antibody levels were compared between groups by an unpaired, two-tailed Mann–Whitney U-test, significant differences are projected using asterisks (*: p < 0.05, **: p < 0.01, ****: p < 0.0001).

Article Snippet: The following antibodies were employed in this work: anti-human IgG-HRP (Biosan, Novosibirsk, Russia, Cat.# I-3021), anti-human IgA-HRP (Invitrogen, Carlsbad, CA, USA, Cat.# SA1-35467), anti-human IgM-HRP (Biosan, Novosibirsk, Russia, Cat.# I-3022), anti-human IgG1 (HyTest, Turku, Finland, Cat.# 1G2cc), anti-human IgG2 (HyTest, Turku, Finland, Cat.# 1G5), anti-human IgG3 (HyTest, Turku, Finland, Cat.# 1G3cc), anti-human IgG4 (HyTest, Turku, Finland, Cat.# 1G4cc), anti-human IgA1-HRP (Invitrogen, Carlsbad, CA, USA, Cat.# SA5-10198), anti-human IgA2-HRP (Invitrogen, Carlsbad, CA, USA, Cat.# SA5-10199), anti-rabbit IgG-HRP (Sigma–Aldrich, Saint Louis, MO, USA, Cat.# A0545), anti-mouse IgG-HRP (Sigma–Aldrich, Saint Louis, MO, USA, Cat.# A2554).

Techniques: Enzyme-linked Immunosorbent Assay, Two Tailed Test, MANN-WHITNEY