Journal: Biomolecules

Article Title: Contactin-1 Is Reduced in Cerebrospinal Fluid of Parkinson’s Disease Patients and Is Present within Lewy Bodies

doi: 10.3390/biom10081177

Figure Lengend Snippet: Levels of cerebrospinal fluid (CSF) contactin-1 ( A ) and contactin-2 ( B ) in PD, DLB and controls. The long horizontal line represents the median and the short horizontal lines represent the inter-quartile range (IQR), respectively. ** p < 0.01. The p -values displayed are corrected for multiple comparisons (Bonferroni correction), sex and age.

Article Snippet: The following primary antibodies were used for evaluation of colocalization using IF between contactin-1/-2 and pathological α-synuclein: rabbit polyclonal anti-contactin-1 (1:40; cat no. ab66265, Abcam, Cambridge, UK), rabbit polyclonal anti-contactin-2 (1:40; HPA001397, Atlas Antibodies, Stockholm, Sweden) in combination with mouse monoclonal anti-p-Ser129-aSyn (11A5 1:30,000, courtesy of Prothena Biosciences [ ]).

Techniques:

Journal: Biomolecules

Article Title: Contactin-1 Is Reduced in Cerebrospinal Fluid of Parkinson’s Disease Patients and Is Present within Lewy Bodies

doi: 10.3390/biom10081177

Figure Lengend Snippet: Correlation of CSF contactin-1 ( A – C ) and contactin-2 ( D – F ) with CSF t-α-syn, tTau and pTau in PD patients. Each dot in the scatter plot represents a sample. r = Spearman’s correlation coefficient.

Article Snippet: The following primary antibodies were used for evaluation of colocalization using IF between contactin-1/-2 and pathological α-synuclein: rabbit polyclonal anti-contactin-1 (1:40; cat no. ab66265, Abcam, Cambridge, UK), rabbit polyclonal anti-contactin-2 (1:40; HPA001397, Atlas Antibodies, Stockholm, Sweden) in combination with mouse monoclonal anti-p-Ser129-aSyn (11A5 1:30,000, courtesy of Prothena Biosciences [ ]).

Techniques:

Journal: Biomolecules

Article Title: Contactin-1 Is Reduced in Cerebrospinal Fluid of Parkinson’s Disease Patients and Is Present within Lewy Bodies

doi: 10.3390/biom10081177

Figure Lengend Snippet: Representative photomicrographs of sections immunostained for contactin-1, contactin-2 and p-Ser129-aSyn in the substantia nigra (SN) of post-mortem human PD brain sections. ( A – C ) Contactin-1 shows punctate staining which co-localizes with p-Ser129-aSyn within Lewy bodies (LBs). ( D – F ) Contactin-1 in bulgy Lewy neurites (LNs). ( B ) A Lewy body and diffuse cytoplasmic (shown by blue arrowhead) stain of p-Ser129-aSyn is visible. ( G – I ) Contactin-2 similarly shows punctate staining, which co-localizes with α-syn within LBs. ( J – L ) Contactin-1 in bulgy LNs. White arrowheads indicate the presence of Contactin-1/-2 within LBs/LNs. Scale bar: 5 uM. Colocalization quantification is shown in .

Article Snippet: The following primary antibodies were used for evaluation of colocalization using IF between contactin-1/-2 and pathological α-synuclein: rabbit polyclonal anti-contactin-1 (1:40; cat no. ab66265, Abcam, Cambridge, UK), rabbit polyclonal anti-contactin-2 (1:40; HPA001397, Atlas Antibodies, Stockholm, Sweden) in combination with mouse monoclonal anti-p-Ser129-aSyn (11A5 1:30,000, courtesy of Prothena Biosciences [ ]).

Techniques: Staining