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    • anti chrm1 antibody  (Alomone Labs)
    92
    Alomone Labs anti chrm1 antibody
    Anti Chrm1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti chrm1 antibody/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti chrm1 antibody - by Bioz Stars, 2023-03
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    amr 010 ab 2340994 rabbit anti m2 muscarinic receptor alomone  (Alomone Labs)
    93
    Alomone Labs amr 010 ab 2340994 rabbit anti m2 muscarinic receptor alomone
    Amr 010 Ab 2340994 Rabbit Anti M2 Muscarinic Receptor Alomone, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amr 010 ab 2340994 rabbit anti m2 muscarinic receptor alomone/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
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    chrm1  (Santa Cruz Biotechnology)
    86
    Santa Cruz Biotechnology chrm1
    ( A ) Immunofluorescent analysis of TUBB3+ nerves as well as SOX2-, SOX10-, <t>CHRM1-</t> and EGFR-expressing cells in fetal human submandibular (SMG) or sublingual (SLG) at 16–24 w. E-cadherin (ECAD) marks epithelial cells. Image of the 16 w SLG is a 20 µm stack of 1 µm confocal sections. All other images are 1–2 µm confocal sections. ( B ) Brightfield images of SLG explants cultured with E13 murine parasympathetic ganglion (PSG) or mesenchyme alone (MES) at day 0 (upper panels) and day 7 (lower panels). ( C–E ) Explants cultured with murine E13 PSG or mesenchyme for 7 days were subjected to immunofluorescent analysis for SOX10, ECAD and TUBB3+ nerves and SOX2 ( C, D ) or gene profiling by qPCR ( E ). Data in E (six biological replicates, two individual experiments) are means+s.d. Scale bar is 50 µm ( A , C (right panel)), 500 µm ( B , C (left panel), 20 µm ( D ). ( F–H ) Analysis of fetal human SLG (22–23 w) dissociated cells ( F and H ) or explants ( G ) cultured ± CCh for 48–72 hr. ( H ) The number of ECAD+SOX2+ (red markers) and ECAD+SOX2+Ki67+ (black markers) cells were measured by FACS as a percentage of cells of total ECAD+ cells. Each line represents an independent experiment. In F and G fold changes in gene expression in dissociated cells or explants were determined via qPCR with expression normalised to GAPDH and control values (dashed line). Data in H were analyzed using a Wilcoxon signed-rank test. Data in F and G were analyzed using a one-way analysis of variance with post-hoc Dunnett’s test. *p<0.05, **p<0.01. Additional data for this figure in . DOI: http://dx.doi.org/10.7554/eLife.26620.031 10.7554/eLife.26620.032 Figure 6—source data 1. Source data relating to . Human fetal SLG explants cultured with murine E13 PSG or mesenchyme for 7 days were subjected to gene profiling by qPCR. Data were normalized to GAPDH and control (+ mesenchyme). Data are means of six biological replicates, two individual experiments. s.d. = standard deviation. DOI: http://dx.doi.org/10.7554/eLife.26620.032 10.7554/eLife.26620.033 Figure 6—source data 2. Source data relating to . qPCR analysis of fetal human SLG (22–23 w) dissociated cells cultured ± CCh for 48 hr. Data were normalized to GAPDH and control (-CCh). Data are means of six biological replicates, two individual experiments. s.d. = standard deviation. DOI: http://dx.doi.org/10.7554/eLife.26620.033 10.7554/eLife.26620.034 Figure 6—source data 3. Source data relating to . qPCR analysis of fetal human SLG (22–23 w) explants cultured ± CCh for 72 hr. Data were normalized to GAPDH and control (-CCh). Data are means of six biological replicates, two individual experiments. s.d. = standard deviation. DOI: http://dx.doi.org/10.7554/eLife.26620.034 10.7554/eLife.26620.035 Figure 6—source data 4. Source data relating to . Analysis of fetal human SLG (22–23 w) dissociated cells cultured ± CCh for 48 hr. The number of ECAD+SOX2+ and ECAD+SOX2+Ki67+ cells were measured by FACS as a percentage of total ECAD+ cells. Each # represents an independent experiment. s.d. = standard deviation. DOI: http://dx.doi.org/10.7554/eLife.26620.035
    Chrm1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chrm1/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    chrm1 - by Bioz Stars, 2023-03
    86/100 stars
    		  Buy from Supplier
    anti chrm1  (Santa Cruz Biotechnology)
    93
    Santa Cruz Biotechnology anti chrm1
    ( A ) Immunofluorescent analysis of TUBB3+ nerves as well as SOX2-, SOX10-, <t>CHRM1-</t> and EGFR-expressing cells in fetal human submandibular (SMG) or sublingual (SLG) at 16–24 w. E-cadherin (ECAD) marks epithelial cells. Image of the 16 w SLG is a 20 µm stack of 1 µm confocal sections. All other images are 1–2 µm confocal sections. ( B ) Brightfield images of SLG explants cultured with E13 murine parasympathetic ganglion (PSG) or mesenchyme alone (MES) at day 0 (upper panels) and day 7 (lower panels). ( C–E ) Explants cultured with murine E13 PSG or mesenchyme for 7 days were subjected to immunofluorescent analysis for SOX10, ECAD and TUBB3+ nerves and SOX2 ( C, D ) or gene profiling by qPCR ( E ). Data in E (six biological replicates, two individual experiments) are means+s.d. Scale bar is 50 µm ( A , C (right panel)), 500 µm ( B , C (left panel), 20 µm ( D ). ( F–H ) Analysis of fetal human SLG (22–23 w) dissociated cells ( F and H ) or explants ( G ) cultured ± CCh for 48–72 hr. ( H ) The number of ECAD+SOX2+ (red markers) and ECAD+SOX2+Ki67+ (black markers) cells were measured by FACS as a percentage of cells of total ECAD+ cells. Each line represents an independent experiment. In F and G fold changes in gene expression in dissociated cells or explants were determined via qPCR with expression normalised to GAPDH and control values (dashed line). Data in H were analyzed using a Wilcoxon signed-rank test. Data in F and G were analyzed using a one-way analysis of variance with post-hoc Dunnett’s test. *p<0.05, **p<0.01. Additional data for this figure in . DOI: http://dx.doi.org/10.7554/eLife.26620.031 10.7554/eLife.26620.032 Figure 6—source data 1. Source data relating to . Human fetal SLG explants cultured with murine E13 PSG or mesenchyme for 7 days were subjected to gene profiling by qPCR. Data were normalized to GAPDH and control (+ mesenchyme). Data are means of six biological replicates, two individual experiments. s.d. = standard deviation. DOI: http://dx.doi.org/10.7554/eLife.26620.032 10.7554/eLife.26620.033 Figure 6—source data 2. Source data relating to . qPCR analysis of fetal human SLG (22–23 w) dissociated cells cultured ± CCh for 48 hr. Data were normalized to GAPDH and control (-CCh). Data are means of six biological replicates, two individual experiments. s.d. = standard deviation. DOI: http://dx.doi.org/10.7554/eLife.26620.033 10.7554/eLife.26620.034 Figure 6—source data 3. Source data relating to . qPCR analysis of fetal human SLG (22–23 w) explants cultured ± CCh for 72 hr. Data were normalized to GAPDH and control (-CCh). Data are means of six biological replicates, two individual experiments. s.d. = standard deviation. DOI: http://dx.doi.org/10.7554/eLife.26620.034 10.7554/eLife.26620.035 Figure 6—source data 4. Source data relating to . Analysis of fetal human SLG (22–23 w) dissociated cells cultured ± CCh for 48 hr. The number of ECAD+SOX2+ and ECAD+SOX2+Ki67+ cells were measured by FACS as a percentage of total ECAD+ cells. Each # represents an independent experiment. s.d. = standard deviation. DOI: http://dx.doi.org/10.7554/eLife.26620.035
    Anti Chrm1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti chrm1/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti chrm1 - by Bioz Stars, 2023-03
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Immunofluorescent analysis of TUBB3+ nerves as well as SOX2-, SOX10-, CHRM1- and EGFR-expressing cells in fetal human submandibular (SMG) or sublingual (SLG) at 16–24 w. E-cadherin (ECAD) marks epithelial cells. Image of the 16 w SLG is a 20 µm stack of 1 µm confocal sections. All other images are 1–2 µm confocal sections. ( B ) Brightfield images of SLG explants cultured with E13 murine parasympathetic ganglion (PSG) or mesenchyme alone (MES) at day 0 (upper panels) and day 7 (lower panels). ( C–E ) Explants cultured with murine E13 PSG or mesenchyme for 7 days were subjected to immunofluorescent analysis for SOX10, ECAD and TUBB3+ nerves and SOX2 ( C, D ) or gene profiling by qPCR ( E ). Data in E (six biological replicates, two individual experiments) are means+s.d. Scale bar is 50 µm ( A , C (right panel)), 500 µm ( B , C (left panel), 20 µm ( D ). ( F–H ) Analysis of fetal human SLG (22–23 w) dissociated cells ( F and H ) or explants ( G ) cultured ± CCh for 48–72 hr. ( H ) The number of ECAD+SOX2+ (red markers) and ECAD+SOX2+Ki67+ (black markers) cells were measured by FACS as a percentage of cells of total ECAD+ cells. Each line represents an independent experiment. In F and G fold changes in gene expression in dissociated cells or explants were determined via qPCR with expression normalised to GAPDH and control values (dashed line). Data in H were analyzed using a Wilcoxon signed-rank test. Data in F and G were analyzed using a one-way analysis of variance with post-hoc Dunnett’s test. *p<0.05, **p<0.01. Additional data for this figure in . DOI: http://dx.doi.org/10.7554/eLife.26620.031 10.7554/eLife.26620.032 Figure 6—source data 1. Source data relating to . Human fetal SLG explants cultured with murine E13 PSG or mesenchyme for 7 days were subjected to gene profiling by qPCR. Data were normalized to GAPDH and control (+ mesenchyme). Data are means of six biological replicates, two individual experiments. s.d. = standard deviation. DOI: http://dx.doi.org/10.7554/eLife.26620.032 10.7554/eLife.26620.033 Figure 6—source data 2. Source data relating to . qPCR analysis of fetal human SLG (22–23 w) dissociated cells cultured ± CCh for 48 hr. Data were normalized to GAPDH and control (-CCh). Data are means of six biological replicates, two individual experiments. s.d. = standard deviation. DOI: http://dx.doi.org/10.7554/eLife.26620.033 10.7554/eLife.26620.034 Figure 6—source data 3. Source data relating to . qPCR analysis of fetal human SLG (22–23 w) explants cultured ± CCh for 72 hr. Data were normalized to GAPDH and control (-CCh). Data are means of six biological replicates, two individual experiments. s.d. = standard deviation. DOI: http://dx.doi.org/10.7554/eLife.26620.034 10.7554/eLife.26620.035 Figure 6—source data 4. Source data relating to . Analysis of fetal human SLG (22–23 w) dissociated cells cultured ± CCh for 48 hr. The number of ECAD+SOX2+ and ECAD+SOX2+Ki67+ cells were measured by FACS as a percentage of total ECAD+ cells. Each # represents an independent experiment. s.d. = standard deviation. DOI: http://dx.doi.org/10.7554/eLife.26620.035

    Journal: eLife

    Article Title: SOX2 regulates acinar cell development in the salivary gland

    doi: 10.7554/eLife.26620

    Figure Lengend Snippet: ( A ) Immunofluorescent analysis of TUBB3+ nerves as well as SOX2-, SOX10-, CHRM1- and EGFR-expressing cells in fetal human submandibular (SMG) or sublingual (SLG) at 16–24 w. E-cadherin (ECAD) marks epithelial cells. Image of the 16 w SLG is a 20 µm stack of 1 µm confocal sections. All other images are 1–2 µm confocal sections. ( B ) Brightfield images of SLG explants cultured with E13 murine parasympathetic ganglion (PSG) or mesenchyme alone (MES) at day 0 (upper panels) and day 7 (lower panels). ( C–E ) Explants cultured with murine E13 PSG or mesenchyme for 7 days were subjected to immunofluorescent analysis for SOX10, ECAD and TUBB3+ nerves and SOX2 ( C, D ) or gene profiling by qPCR ( E ). Data in E (six biological replicates, two individual experiments) are means+s.d. Scale bar is 50 µm ( A , C (right panel)), 500 µm ( B , C (left panel), 20 µm ( D ). ( F–H ) Analysis of fetal human SLG (22–23 w) dissociated cells ( F and H ) or explants ( G ) cultured ± CCh for 48–72 hr. ( H ) The number of ECAD+SOX2+ (red markers) and ECAD+SOX2+Ki67+ (black markers) cells were measured by FACS as a percentage of cells of total ECAD+ cells. Each line represents an independent experiment. In F and G fold changes in gene expression in dissociated cells or explants were determined via qPCR with expression normalised to GAPDH and control values (dashed line). Data in H were analyzed using a Wilcoxon signed-rank test. Data in F and G were analyzed using a one-way analysis of variance with post-hoc Dunnett’s test. *p<0.05, **p<0.01. Additional data for this figure in . DOI: http://dx.doi.org/10.7554/eLife.26620.031 10.7554/eLife.26620.032 Figure 6—source data 1. Source data relating to . Human fetal SLG explants cultured with murine E13 PSG or mesenchyme for 7 days were subjected to gene profiling by qPCR. Data were normalized to GAPDH and control (+ mesenchyme). Data are means of six biological replicates, two individual experiments. s.d. = standard deviation. DOI: http://dx.doi.org/10.7554/eLife.26620.032 10.7554/eLife.26620.033 Figure 6—source data 2. Source data relating to . qPCR analysis of fetal human SLG (22–23 w) dissociated cells cultured ± CCh for 48 hr. Data were normalized to GAPDH and control (-CCh). Data are means of six biological replicates, two individual experiments. s.d. = standard deviation. DOI: http://dx.doi.org/10.7554/eLife.26620.033 10.7554/eLife.26620.034 Figure 6—source data 3. Source data relating to . qPCR analysis of fetal human SLG (22–23 w) explants cultured ± CCh for 72 hr. Data were normalized to GAPDH and control (-CCh). Data are means of six biological replicates, two individual experiments. s.d. = standard deviation. DOI: http://dx.doi.org/10.7554/eLife.26620.034 10.7554/eLife.26620.035 Figure 6—source data 4. Source data relating to . Analysis of fetal human SLG (22–23 w) dissociated cells cultured ± CCh for 48 hr. The number of ECAD+SOX2+ and ECAD+SOX2+Ki67+ cells were measured by FACS as a percentage of total ECAD+ cells. Each # represents an independent experiment. s.d. = standard deviation. DOI: http://dx.doi.org/10.7554/eLife.26620.035

    Article Snippet: Cell surface staining was achieved by incubating cell suspensions with antibodies against CD324 (ECAD; eBioscience, 46-3249-80; AB_1834418), CD326 (EpCAM; Miltenyi, 130-098-113; AB_2660298) and CHRM1 (Santa Cruz, sc-7470; AB_2079955).

    Techniques: Expressing, Cell Culture, Standard Deviation

    Sequences for mouse primers used for qPCR. DOI: http://dx.doi.org/10.7554/eLife.26620.037

    Journal: eLife

    Article Title: SOX2 regulates acinar cell development in the salivary gland

    doi: 10.7554/eLife.26620

    Figure Lengend Snippet: Sequences for mouse primers used for qPCR. DOI: http://dx.doi.org/10.7554/eLife.26620.037

    Article Snippet: Cell surface staining was achieved by incubating cell suspensions with antibodies against CD324 (ECAD; eBioscience, 46-3249-80; AB_1834418), CD326 (EpCAM; Miltenyi, 130-098-113; AB_2660298) and CHRM1 (Santa Cruz, sc-7470; AB_2079955).

    Techniques: Sequencing

    Sequences for human primers used for qPCR. DOI: http://dx.doi.org/10.7554/eLife.26620.038

    Journal: eLife

    Article Title: SOX2 regulates acinar cell development in the salivary gland

    doi: 10.7554/eLife.26620

    Figure Lengend Snippet: Sequences for human primers used for qPCR. DOI: http://dx.doi.org/10.7554/eLife.26620.038

    Article Snippet: Cell surface staining was achieved by incubating cell suspensions with antibodies against CD324 (ECAD; eBioscience, 46-3249-80; AB_1834418), CD326 (EpCAM; Miltenyi, 130-098-113; AB_2660298) and CHRM1 (Santa Cruz, sc-7470; AB_2079955).

    Techniques: Sequencing