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  • 99
    Thermo Fisher anti cd8
    mDTC-associated PD-1+ <t>CD8+</t> and CD4+ T cells display a molecular profile indicative of exhaustion
    Anti Cd8, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd8/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd8 - by Bioz Stars, 2022-09
    99/100 stars
      Buy from Supplier

    97
    Becton Dickinson anti cd8
    mDTC-associated PD-1+ <t>CD8+</t> and CD4+ T cells display a molecular profile indicative of exhaustion
    Anti Cd8, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd8/product/Becton Dickinson
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd8 - by Bioz Stars, 2022-09
    97/100 stars
      Buy from Supplier

    88
    Thermo Fisher anti cd3
    Expression of cytotoxic molecules by in vitro stimulated T and NK cells of PBMCs from cART treated PLWH. PBMCs from cART-treated PLWH were stimulated either with 200 ng/ml SEB or with 1 µg/ml of an HLA class I restricted HIV peptide pool in presence of 2000 U/ml IFNα2, IFNα14, IFNβ, or without IFN (-IFN) for 4 days. PBMCs were re-stimulated with 5 µg/ml SEB or 1 µg/ml peptide pool respectively and incubated in presence of <t>antibodies</t> against the co-stimulatory molecules CD28 and CD49d for 6 h BFA was added after 1 h of stimulation. Flow cytometry was used to analyze T cell activation and cytokine expression. (A) Activation profile determined by the frequencies of HLA-DR + <t>CD4</t> + and <t>CD8</t> + T cells with or without stimulation in the presence or absence of the different IFNs. (B–E) Frequencies of the cytotoxic molecules CD107a, TRAIL, GzmB, and IFNγ expressed on CD4 + , CD8 + T cells, and <t>CD56</t> + NK cells. Mean values ± SEM are shown for (A–C) n=6 and (D, E) n=3. Statistical analyses between the treated groups within a cell population were done by using Friedman test and Dunn’s multiple comparison test. *, P
    Anti Cd3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd3/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd3 - by Bioz Stars, 2022-09
    88/100 stars
      Buy from Supplier

    93
    BioLegend anti cd8
    Expression of cytotoxic molecules by in vitro stimulated T and NK cells of PBMCs from cART treated PLWH. PBMCs from cART-treated PLWH were stimulated either with 200 ng/ml SEB or with 1 µg/ml of an HLA class I restricted HIV peptide pool in presence of 2000 U/ml IFNα2, IFNα14, IFNβ, or without IFN (-IFN) for 4 days. PBMCs were re-stimulated with 5 µg/ml SEB or 1 µg/ml peptide pool respectively and incubated in presence of <t>antibodies</t> against the co-stimulatory molecules CD28 and CD49d for 6 h BFA was added after 1 h of stimulation. Flow cytometry was used to analyze T cell activation and cytokine expression. (A) Activation profile determined by the frequencies of HLA-DR + <t>CD4</t> + and <t>CD8</t> + T cells with or without stimulation in the presence or absence of the different IFNs. (B–E) Frequencies of the cytotoxic molecules CD107a, TRAIL, GzmB, and IFNγ expressed on CD4 + , CD8 + T cells, and <t>CD56</t> + NK cells. Mean values ± SEM are shown for (A–C) n=6 and (D, E) n=3. Statistical analyses between the treated groups within a cell population were done by using Friedman test and Dunn’s multiple comparison test. *, P
    Anti Cd8, supplied by BioLegend, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd8/product/BioLegend
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd8 - by Bioz Stars, 2022-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    mDTC-associated PD-1+ CD8+ and CD4+ T cells display a molecular profile indicative of exhaustion

    Journal: Cancer immunology research

    Article Title: PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer

    doi: 10.1158/2326-6066.CIR-14-0201

    Figure Lengend Snippet: mDTC-associated PD-1+ CD8+ and CD4+ T cells display a molecular profile indicative of exhaustion

    Article Snippet: For analysis of T-cell exhaustion, cells were split and stained with the following antibody combinations: (1) anti-CD3-Alexafluor700, anti-CD4-Fitc, anti-CD8, anti-PD-1, anti-TIM-3, anti-CD127, anti-CD27-APC-efluor780, and anti-CD69-biotin or (2) anti-CD3-Alexafluor700, anti-CD4-APC-efluor780, anti-CD8, anti-PD-1, anti-TIM-3, and anti-LAG-3 for 25 minutes at 4 ° C. Anti-CD69-biotin was detected with Streptavidin-PE-TR (15 minutes at 4 ° C; eBioscience).

    Techniques:

    Cytotoxic potential of CD8 + T cells from PD-1 + Tim-3 + TILNs

    Journal: Cancer immunology research

    Article Title: PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer

    doi: 10.1158/2326-6066.CIR-14-0201

    Figure Lengend Snippet: Cytotoxic potential of CD8 + T cells from PD-1 + Tim-3 + TILNs

    Article Snippet: For analysis of T-cell exhaustion, cells were split and stained with the following antibody combinations: (1) anti-CD3-Alexafluor700, anti-CD4-Fitc, anti-CD8, anti-PD-1, anti-TIM-3, anti-CD127, anti-CD27-APC-efluor780, and anti-CD69-biotin or (2) anti-CD3-Alexafluor700, anti-CD4-APC-efluor780, anti-CD8, anti-PD-1, anti-TIM-3, and anti-LAG-3 for 25 minutes at 4 ° C. Anti-CD69-biotin was detected with Streptavidin-PE-TR (15 minutes at 4 ° C; eBioscience).

    Techniques:

    Expression of cytotoxic molecules by in vitro stimulated T and NK cells of PBMCs from cART treated PLWH. PBMCs from cART-treated PLWH were stimulated either with 200 ng/ml SEB or with 1 µg/ml of an HLA class I restricted HIV peptide pool in presence of 2000 U/ml IFNα2, IFNα14, IFNβ, or without IFN (-IFN) for 4 days. PBMCs were re-stimulated with 5 µg/ml SEB or 1 µg/ml peptide pool respectively and incubated in presence of antibodies against the co-stimulatory molecules CD28 and CD49d for 6 h BFA was added after 1 h of stimulation. Flow cytometry was used to analyze T cell activation and cytokine expression. (A) Activation profile determined by the frequencies of HLA-DR + CD4 + and CD8 + T cells with or without stimulation in the presence or absence of the different IFNs. (B–E) Frequencies of the cytotoxic molecules CD107a, TRAIL, GzmB, and IFNγ expressed on CD4 + , CD8 + T cells, and CD56 + NK cells. Mean values ± SEM are shown for (A–C) n=6 and (D, E) n=3. Statistical analyses between the treated groups within a cell population were done by using Friedman test and Dunn’s multiple comparison test. *, P

    Journal: Frontiers in Immunology

    Article Title: Distinct Type I Interferon Subtypes Differentially Stimulate T Cell Responses in HIV-1-Infected Individuals

    doi: 10.3389/fimmu.2022.936918

    Figure Lengend Snippet: Expression of cytotoxic molecules by in vitro stimulated T and NK cells of PBMCs from cART treated PLWH. PBMCs from cART-treated PLWH were stimulated either with 200 ng/ml SEB or with 1 µg/ml of an HLA class I restricted HIV peptide pool in presence of 2000 U/ml IFNα2, IFNα14, IFNβ, or without IFN (-IFN) for 4 days. PBMCs were re-stimulated with 5 µg/ml SEB or 1 µg/ml peptide pool respectively and incubated in presence of antibodies against the co-stimulatory molecules CD28 and CD49d for 6 h BFA was added after 1 h of stimulation. Flow cytometry was used to analyze T cell activation and cytokine expression. (A) Activation profile determined by the frequencies of HLA-DR + CD4 + and CD8 + T cells with or without stimulation in the presence or absence of the different IFNs. (B–E) Frequencies of the cytotoxic molecules CD107a, TRAIL, GzmB, and IFNγ expressed on CD4 + , CD8 + T cells, and CD56 + NK cells. Mean values ± SEM are shown for (A–C) n=6 and (D, E) n=3. Statistical analyses between the treated groups within a cell population were done by using Friedman test and Dunn’s multiple comparison test. *, P

    Article Snippet: Surface staining was performed simultaneously to IFN stimulation with the following antibodies: anti-CD3 (UCHT1, eBioscience™), anti-CD4 (RPA-T4, BioLegend), anti-CD8 (RPA-T8, BioLegend), anti-CD56 (HCD56, BioLegend), as well as FVD.

    Techniques: Expressing, In Vitro, Incubation, Flow Cytometry, Activation Assay

    Phosphorylation of STAT molecules after IFN stimulation in PBMCs from PLWH. PBMCs from PLWH were stimulated with 2000 U/ml IFNα2, IFNα14, IFNβ, or without IFN (-IFN) in presence of the surface markers anti-CD3, anti-CD4, anti-CD8, anti-CD56, and the viability marker FVD. Cells were then fixated and permeabilized for phosphostaining with anti-STAT1 pTyr702, anti-STAT5 pTyr694, anti-STAT3 pTyr705, and anti-STAT3 pSer727. (A) Frequencies of phosphorylated STAT1, (B) mean donor-specific fold changes of MFI (pSTAT1) (C) frequencies of phosphorylated STAT3 pTyr705, (D) STAT3 pSer727, (E) STAT5, and (F) mean donor-specific fold changes of MFI (pSTAT5) on CD4 + , CD8 + T, and NK cells. Mean values ± SEM are shown for n=5. Statistical analyses between the treated groups within a cell population were done by using Friedman test and Dunn’s multiple comparison test. **, P

    Journal: Frontiers in Immunology

    Article Title: Distinct Type I Interferon Subtypes Differentially Stimulate T Cell Responses in HIV-1-Infected Individuals

    doi: 10.3389/fimmu.2022.936918

    Figure Lengend Snippet: Phosphorylation of STAT molecules after IFN stimulation in PBMCs from PLWH. PBMCs from PLWH were stimulated with 2000 U/ml IFNα2, IFNα14, IFNβ, or without IFN (-IFN) in presence of the surface markers anti-CD3, anti-CD4, anti-CD8, anti-CD56, and the viability marker FVD. Cells were then fixated and permeabilized for phosphostaining with anti-STAT1 pTyr702, anti-STAT5 pTyr694, anti-STAT3 pTyr705, and anti-STAT3 pSer727. (A) Frequencies of phosphorylated STAT1, (B) mean donor-specific fold changes of MFI (pSTAT1) (C) frequencies of phosphorylated STAT3 pTyr705, (D) STAT3 pSer727, (E) STAT5, and (F) mean donor-specific fold changes of MFI (pSTAT5) on CD4 + , CD8 + T, and NK cells. Mean values ± SEM are shown for n=5. Statistical analyses between the treated groups within a cell population were done by using Friedman test and Dunn’s multiple comparison test. **, P

    Article Snippet: Surface staining was performed simultaneously to IFN stimulation with the following antibodies: anti-CD3 (UCHT1, eBioscience™), anti-CD4 (RPA-T4, BioLegend), anti-CD8 (RPA-T8, BioLegend), anti-CD56 (HCD56, BioLegend), as well as FVD.

    Techniques: Marker

    Phosphorylation of STAT molecules after IFN stimulation in LPMCs. LPMCs were stimulated with 2000 U/ml IFNα2, IFNα14, IFNβ, or without IFN (-IFN) in presence of the surface markers anti-CD3, anti-CD4, anti-CD8, anti-CD56, and the viability marker FVD. Cells were then fixated and permeabilized for phosphoflow analysis with anti-STAT1 pTyr702, anti-STAT5 pTyr694, anti-STAT3 pTyr705, and anti-STAT3 pSer727. (A) Frequencies of phosphorylated STAT1, (B) mean donor-specific fold changes of MFI (pSTAT1) (C) frequencies of phosphorylated STAT3 pTyr705, (D) STAT3 pSer727, and (E) STAT5 on CD4 + , CD8 + T, and NK cells. Mean values ± SEM are shown for n=6. Statistical analyses between the treated groups within a cell population were done by using Friedman test and Dunn’s multiple comparison test. ***, P

    Journal: Frontiers in Immunology

    Article Title: Distinct Type I Interferon Subtypes Differentially Stimulate T Cell Responses in HIV-1-Infected Individuals

    doi: 10.3389/fimmu.2022.936918

    Figure Lengend Snippet: Phosphorylation of STAT molecules after IFN stimulation in LPMCs. LPMCs were stimulated with 2000 U/ml IFNα2, IFNα14, IFNβ, or without IFN (-IFN) in presence of the surface markers anti-CD3, anti-CD4, anti-CD8, anti-CD56, and the viability marker FVD. Cells were then fixated and permeabilized for phosphoflow analysis with anti-STAT1 pTyr702, anti-STAT5 pTyr694, anti-STAT3 pTyr705, and anti-STAT3 pSer727. (A) Frequencies of phosphorylated STAT1, (B) mean donor-specific fold changes of MFI (pSTAT1) (C) frequencies of phosphorylated STAT3 pTyr705, (D) STAT3 pSer727, and (E) STAT5 on CD4 + , CD8 + T, and NK cells. Mean values ± SEM are shown for n=6. Statistical analyses between the treated groups within a cell population were done by using Friedman test and Dunn’s multiple comparison test. ***, P

    Article Snippet: Surface staining was performed simultaneously to IFN stimulation with the following antibodies: anti-CD3 (UCHT1, eBioscience™), anti-CD4 (RPA-T4, BioLegend), anti-CD8 (RPA-T8, BioLegend), anti-CD56 (HCD56, BioLegend), as well as FVD.

    Techniques: Marker

    Phosphorylation of STAT molecules after IFN stimulation in PBMCs. PBMCs from healthy donors were stimulated with 2000 U/ml IFNα2, IFNα14, IFNβ, or without IFN (-IFN) in presence of the surface markers anti-CD3, anti-CD4, anti-CD8, anti-CD56, and the viability marker FVD. Cells were then fixated and permeabilized for phosphostaining with anti-STAT1 pTyr702, anti-STAT5 pTyr694, anti-STAT3 pTyr705, and anti-STAT3 pSer727. (A) Frequencies of phosphorylated STAT1, (B) mean donor-specific fold changes of MFI (pSTAT1) (C) frequencies of phosphorylated STAT3 pTyr705, (D) STAT3 pSer727, (E) STAT5, and (F) mean donor-specific fold changes of MFI (pSTAT5) on CD4 + , CD8 + T, and NK cells. Mean values ± SEM are shown for n=6. Statistical analyses between the treated groups within a cell population were done by using Friedman test and Dunn’s multiple comparison test. **, P

    Journal: Frontiers in Immunology

    Article Title: Distinct Type I Interferon Subtypes Differentially Stimulate T Cell Responses in HIV-1-Infected Individuals

    doi: 10.3389/fimmu.2022.936918

    Figure Lengend Snippet: Phosphorylation of STAT molecules after IFN stimulation in PBMCs. PBMCs from healthy donors were stimulated with 2000 U/ml IFNα2, IFNα14, IFNβ, or without IFN (-IFN) in presence of the surface markers anti-CD3, anti-CD4, anti-CD8, anti-CD56, and the viability marker FVD. Cells were then fixated and permeabilized for phosphostaining with anti-STAT1 pTyr702, anti-STAT5 pTyr694, anti-STAT3 pTyr705, and anti-STAT3 pSer727. (A) Frequencies of phosphorylated STAT1, (B) mean donor-specific fold changes of MFI (pSTAT1) (C) frequencies of phosphorylated STAT3 pTyr705, (D) STAT3 pSer727, (E) STAT5, and (F) mean donor-specific fold changes of MFI (pSTAT5) on CD4 + , CD8 + T, and NK cells. Mean values ± SEM are shown for n=6. Statistical analyses between the treated groups within a cell population were done by using Friedman test and Dunn’s multiple comparison test. **, P

    Article Snippet: Surface staining was performed simultaneously to IFN stimulation with the following antibodies: anti-CD3 (UCHT1, eBioscience™), anti-CD4 (RPA-T4, BioLegend), anti-CD8 (RPA-T8, BioLegend), anti-CD56 (HCD56, BioLegend), as well as FVD.

    Techniques: Marker