Journal: Viruses

Article Title: Serum-Derived Extracellular Vesicles from African Swine Fever Virus-Infected Pigs Selectively Recruit Viral and Porcine Proteins

doi: 10.3390/v11100882

Figure Lengend Snippet: FACS analysis of EV-enriched fractions derived from infected and control animals. ( a ) EVs from representative sera of an infected animal with OURT 88/3 at 24 days post infection, ( b ) EV profiles from a representative of uninfected swine sera, ( c ) EV FACS profile from a representative animal infected with Benin ΔMGF virus. In all samples, molecular markers CD5 and CD163 were used as control markers for EVs and three different viral proteins were evaluated (p30, p54 and p72).

Article Snippet: Fractions were incubated in microtest conical-bottom 96-well plates for 30 min at 4 °C with anti-CD63 (Clone H5C6) and anti-CD81 (Clone JS-81) antibodies (BD Biosciences, San Jose, CA, USA) at 1:100 dilution, anti-CD5 (clone PG114A, Kingfisher Biotech, St Paul, MN, USA) at 1:200 or anti-CD163 (clone 2A10 gently given by Dr. Javier Dominguez).

Techniques: Derivative Assay, Infection

Journal: Cell reports

Article Title: A Two-Step Process of Effector Programming Governs CD4 + T Cell Fate Determination Induced by Antigenic Activation in the Steady State

doi: 10.1016/j.celrep.2020.108424

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: anti-CD5 , BioLegend , 53–7.3 RRID: AB_312733.

Techniques: Purification, Recombinant, Staining, Isolation, Protease Inhibitor, Software