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Image Search Results

Journal: Viruses
Article Title: Heat Shock Enhances the Expression of the Human T Cell Leukemia Virus Type-I (HTLV-I) Trans-Activator (Tax) Antigen in Human HTLV-I Infected Primary and Cultured T Cells
doi: 10.3390/v8070191
Figure Lengend Snippet: Effects of heat shock (HS) exposure on human T cell leukemia virus type-I (HTLV-I)-infected cell lines derived from acute adult T cell leukemia (ATL) patients: ( a ) Aliquots of ATL-026i and ATL-056i cells were incubated at various temperatures for 30 min and cultured for 24 h. The cells were then analyzed for the frequencies of trans-activator (Tax) + cells (left bar graphs) by flow cytometry (FCM) and the relative density (Mean Fluorescent Intensity, MFI) of heat shock protein 70 (HSP70) expression (middle bar graphs) and for cell viability using the CCK-8 cell counting kit (right bar graphs). ( b ) The kinetics of the up-regulation of Tax and HSP70 expression by the same two cell lines following exposure to 43 °C for various times is shown. The values denote the means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet:
Techniques: Infection, Derivative Assay, Incubation, Cell Culture, Flow Cytometry, Expressing, CCK-8 Assay, Cell Counting

Journal: Viruses
Article Title: Heat Shock Enhances the Expression of the Human T Cell Leukemia Virus Type-I (HTLV-I) Trans-Activator (Tax) Antigen in Human HTLV-I Infected Primary and Cultured T Cells
doi: 10.3390/v8070191
Figure Lengend Snippet: A comparison of time course of the expression among HSP70, Tax and gp46 antigens followed by exposure to HS: The ATL-026i (upper panel) and YT/cM1 (lower panel) cells were either exposed to HS (black square) or mock treated (open square), cultured for 12, 24 and 48 h, and then their phenotypes were analyzed by FCM. Data shown are representative of three independent experiments.
Article Snippet:
Techniques: Expressing, Cell Culture

Journal: Viruses
Article Title: Heat Shock Enhances the Expression of the Human T Cell Leukemia Virus Type-I (HTLV-I) Trans-Activator (Tax) Antigen in Human HTLV-I Infected Primary and Cultured T Cells
doi: 10.3390/v8070191
Figure Lengend Snippet: The effects of the HSP70 inducing and inhibiting agents on the expression of Tax antigen: Triplicate cultures of YT/cM1 cells were either exposed to HS or mock treated, and then cultured in the presence or absence of either ( a ) 10 μM zerumbone (ZER, HSP70 inducer) or ( b ) 1 μM pifithlin-μ (PFT, HSP70 functional inhibitor) for 24 h. The Tax and HSP70 expression was analyzed by FCM. The values denote the means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet:
Techniques: Expressing, Cell Culture, Functional Assay