anti γ h2ax Merck Kgaa Search Results


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  • 93
    Merck KGaA anti γ h2ax
    DNA damage after IR increased following treatment with RGD/P-AuNPs. Notes: ( A ) Representative confocal immunofluorescence images of <t>γ-H2AX</t> (red) in MDA-MB-231 cells after treatment with AuNPs and radiation (0 Gy or 4 Gy). Bar, 20 μm. ( B ) Number of γ-H2AX foci per nucleus in MDA-MB-231 cells was counted. Cells pre-cultured with AuNPs were fixed and stained with γ-H2AX antibody 12 h after IR (0 Gy or 4 Gy). At least 50 nuclei were counted in each independent experiment. Columns, mean (n=3), bars, SE, * P
    Anti γ H2ax, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Merck KGaA mouse anti γ h2ax
    DNA damage after IR increased following treatment with RGD/P-AuNPs. Notes: ( A ) Representative confocal immunofluorescence images of <t>γ-H2AX</t> (red) in MDA-MB-231 cells after treatment with AuNPs and radiation (0 Gy or 4 Gy). Bar, 20 μm. ( B ) Number of γ-H2AX foci per nucleus in MDA-MB-231 cells was counted. Cells pre-cultured with AuNPs were fixed and stained with γ-H2AX antibody 12 h after IR (0 Gy or 4 Gy). At least 50 nuclei were counted in each independent experiment. Columns, mean (n=3), bars, SE, * P
    Mouse Anti γ H2ax, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 83/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti γ h2ax/product/Merck KGaA
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    86
    Merck KGaA γ h2ax primary antibody
    DNA damage after IR increased following treatment with RGD/P-AuNPs. Notes: ( A ) Representative confocal immunofluorescence images of <t>γ-H2AX</t> (red) in MDA-MB-231 cells after treatment with AuNPs and radiation (0 Gy or 4 Gy). Bar, 20 μm. ( B ) Number of γ-H2AX foci per nucleus in MDA-MB-231 cells was counted. Cells pre-cultured with AuNPs were fixed and stained with γ-H2AX antibody 12 h after IR (0 Gy or 4 Gy). At least 50 nuclei were counted in each independent experiment. Columns, mean (n=3), bars, SE, * P
    γ H2ax Primary Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/γ h2ax primary antibody/product/Merck KGaA
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    97
    Merck KGaA anti γ h2ax rabbit polyclonal antibody
    (A) Western blotting analysis of wild-type and S260A-mutated XRCC4 in M10-derived transformants. Proliferating cell nuclear antigen (PCNA) is shown as the loading control. (B) Radiosensitivity of M10-XRCC4 WT , M10-XRCC4 S320A and M10-CMV cells measured by colony formation assay in 0.2% w/v agarose. Each symbol represents the average surviving fraction obtained from 8–10 repeated experiments, with the error bar indicating standard deviation. The P value was calculated by applying the data to Welch’s one-sided t -test. * P = 0.049, ** P = 0.023, *** P = 0.017. (C): Number of <t>γ-H2AX</t> foci per cell in M10-XRCC4 WT , M10-XRCC4 S320A and M10-CMV cells. Cells were harvested 4 h after 1 Gy irradiation or without irradiation and subjected to immunostaining using anti-γ-H2AX antibody and fluorescent-labeled secondary antibody. More than 60 cells were examined for each group. Each circle represents the number of foci in a single cell, and the horizontal bar shows the average number of foci per cell in the group. The P -value was calculated by applying the data to Welch’s one-sided t -test.
    Anti γ H2ax Rabbit Polyclonal Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 97/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti γ h2ax rabbit polyclonal antibody/product/Merck KGaA
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    81
    Merck KGaA 488 alex fluor anti γ h2ax antibody
    (A) Western blotting analysis of wild-type and S260A-mutated XRCC4 in M10-derived transformants. Proliferating cell nuclear antigen (PCNA) is shown as the loading control. (B) Radiosensitivity of M10-XRCC4 WT , M10-XRCC4 S320A and M10-CMV cells measured by colony formation assay in 0.2% w/v agarose. Each symbol represents the average surviving fraction obtained from 8–10 repeated experiments, with the error bar indicating standard deviation. The P value was calculated by applying the data to Welch’s one-sided t -test. * P = 0.049, ** P = 0.023, *** P = 0.017. (C): Number of <t>γ-H2AX</t> foci per cell in M10-XRCC4 WT , M10-XRCC4 S320A and M10-CMV cells. Cells were harvested 4 h after 1 Gy irradiation or without irradiation and subjected to immunostaining using anti-γ-H2AX antibody and fluorescent-labeled secondary antibody. More than 60 cells were examined for each group. Each circle represents the number of foci in a single cell, and the horizontal bar shows the average number of foci per cell in the group. The P -value was calculated by applying the data to Welch’s one-sided t -test.
    488 Alex Fluor Anti γ H2ax Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 81/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/488 alex fluor anti γ h2ax antibody/product/Merck KGaA
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    81
    Merck KGaA γ h2ax antibody fitc conjugate
    (A) Western blotting analysis of wild-type and S260A-mutated XRCC4 in M10-derived transformants. Proliferating cell nuclear antigen (PCNA) is shown as the loading control. (B) Radiosensitivity of M10-XRCC4 WT , M10-XRCC4 S320A and M10-CMV cells measured by colony formation assay in 0.2% w/v agarose. Each symbol represents the average surviving fraction obtained from 8–10 repeated experiments, with the error bar indicating standard deviation. The P value was calculated by applying the data to Welch’s one-sided t -test. * P = 0.049, ** P = 0.023, *** P = 0.017. (C): Number of <t>γ-H2AX</t> foci per cell in M10-XRCC4 WT , M10-XRCC4 S320A and M10-CMV cells. Cells were harvested 4 h after 1 Gy irradiation or without irradiation and subjected to immunostaining using anti-γ-H2AX antibody and fluorescent-labeled secondary antibody. More than 60 cells were examined for each group. Each circle represents the number of foci in a single cell, and the horizontal bar shows the average number of foci per cell in the group. The P -value was calculated by applying the data to Welch’s one-sided t -test.
    γ H2ax Antibody Fitc Conjugate, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 81/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Merck KGaA antibodies against γ h2ax fitc
    DSBs were analysed using the <t>γ-H2AX</t> assay ( A ) The induction of DSBs in R1 and SiHa was significantly higher after addition of PARP1- i to cDDP-based thermochermotherapy. In HeLa cells this was not found to be significant, although a trend is seen. R1: p = 0.048, SiHa: p = 0.035, HeLa: p = 0.068 From left to right: R1, SiHa, Hela cells. ( B ) One representative cell is depicted for each condition. Bars represent the mean of three independent experiments with the standard error of the mean (SEM). * p
    Antibodies Against γ H2ax Fitc, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 83/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against γ h2ax fitc/product/Merck KGaA
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    87
    Merck KGaA phosphorylated h2ax
    DSBs were analysed using the <t>γ-H2AX</t> assay ( A ) The induction of DSBs in R1 and SiHa was significantly higher after addition of PARP1- i to cDDP-based thermochermotherapy. In HeLa cells this was not found to be significant, although a trend is seen. R1: p = 0.048, SiHa: p = 0.035, HeLa: p = 0.068 From left to right: R1, SiHa, Hela cells. ( B ) One representative cell is depicted for each condition. Bars represent the mean of three independent experiments with the standard error of the mean (SEM). * p
    Phosphorylated H2ax, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 87/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    DNA damage after IR increased following treatment with RGD/P-AuNPs. Notes: ( A ) Representative confocal immunofluorescence images of γ-H2AX (red) in MDA-MB-231 cells after treatment with AuNPs and radiation (0 Gy or 4 Gy). Bar, 20 μm. ( B ) Number of γ-H2AX foci per nucleus in MDA-MB-231 cells was counted. Cells pre-cultured with AuNPs were fixed and stained with γ-H2AX antibody 12 h after IR (0 Gy or 4 Gy). At least 50 nuclei were counted in each independent experiment. Columns, mean (n=3), bars, SE, * P

    Journal: International Journal of Nanomedicine

    Article Title: Targeting integrins with RGD-conjugated gold nanoparticles in radiotherapy decreases the invasive activity of breast cancer cells

    doi: 10.2147/IJN.S137833

    Figure Lengend Snippet: DNA damage after IR increased following treatment with RGD/P-AuNPs. Notes: ( A ) Representative confocal immunofluorescence images of γ-H2AX (red) in MDA-MB-231 cells after treatment with AuNPs and radiation (0 Gy or 4 Gy). Bar, 20 μm. ( B ) Number of γ-H2AX foci per nucleus in MDA-MB-231 cells was counted. Cells pre-cultured with AuNPs were fixed and stained with γ-H2AX antibody 12 h after IR (0 Gy or 4 Gy). At least 50 nuclei were counted in each independent experiment. Columns, mean (n=3), bars, SE, * P

    Article Snippet: The following primary antibodies were used for immunofluorescence staining: anti-α5-integrin (Cell Signaling Technology), anti-αv-integrin (Cell Signaling Technology), anti-LAMP1 (Cell Signaling Technology), anti-EEA1 (Cell Signaling Technology), anti-Rab5 (Cell Signaling Technology), anti-Rab7 (Cell Signaling Technology), anti-Rab9 (Cell Signaling Technology) and anti-γ-H2AX (Merck Millipore).

    Techniques: Immunofluorescence, Multiple Displacement Amplification, Cell Culture, Staining

    Histopathological analysis of H460 tumors following combination treatment with oridonin and radiation. Hematoxylin and eosin (H-E) staining and immunohistochemistry for cleaved caspase-3 and γ-H2AX were performed on tumors harvested at 14 days after IR. Representative images of H-E-stained tumors ( upper images ) and cleaved caspase-3- and γ-H2AX-positive cells ( middle images , brown staining) and quantification of cleaved caspase-3 and γ-H2AX-positive staining with six mice in each group ( lower plots , means ± SEM) are shown; * p

    Journal: International Journal of Molecular Sciences

    Article Title: Oridonin Enhances Radiation-Induced Cell Death by Promoting DNA Damage in Non-Small Cell Lung Cancer Cells

    doi: 10.3390/ijms19082378

    Figure Lengend Snippet: Histopathological analysis of H460 tumors following combination treatment with oridonin and radiation. Hematoxylin and eosin (H-E) staining and immunohistochemistry for cleaved caspase-3 and γ-H2AX were performed on tumors harvested at 14 days after IR. Representative images of H-E-stained tumors ( upper images ) and cleaved caspase-3- and γ-H2AX-positive cells ( middle images , brown staining) and quantification of cleaved caspase-3 and γ-H2AX-positive staining with six mice in each group ( lower plots , means ± SEM) are shown; * p

    Article Snippet: The specific antibodies were cleaved caspase-3 (1:1000, #9661, Cell Signaling Technology, Inc., Danvers, MA, USA), γ-H2AX (1:3000, 05-636, Merck KGaA, Darmstadt, Germany), and β-actin (1:5000, sc-47778, Santa Cruz Biotechnology, Inc., Dallas, TX, USA).

    Techniques: Staining, Immunohistochemistry, Mouse Assay

    BO-1055 induces DDR and cell death A. Immunoblots showing DDR through the detection of the phosphorylation of ATM Ser1981(ATM-S1981p), Chk1 Ser 345 (Chk1-S345p), or Chk2 Thr 68 (Chk2-T68p), following the exposure of MCF-7 cells to 5, 10, or 20 μM of BO-1055 for 6-h. Cells treated with 0.1 mM of H 2 O 2 and 10 J/m 2 of UV for 30 min served as positive controls. B. The same experiment described in (A), cells were exposed to 5 μM of MMC or of BO-1055 for 0, 1, 6, or 12 hours. C. Immunohistochemical staining for the DNA damage marker γ-H2AX (green) and nucleus DAPI (blue) of cultured MCF-7 cells was conducted following incubation with 5 μM of MMC or BO-1055 for 24-h. D. FACS histogram analysis of DNA content. PI staining in fixed cells was performed following the exposure of cultured MCF-7 cells to the indicated doses of MMC or BO-1055 for the indicated times. E. FACS dot-blot analysis for cell death. AnnexinV/PI double staining in living cells was conducted following the exposure of cultured MCF-7 cells to 5 μM of MMC or of BO-1055 for the indicated times. The experiment of (D) and (E) were performed three times, and the quantitative results expressed as the mean ± SEM are respectively presented in Supplementary Figure S2A and S2B . The cell death, assessed in cells treated with 20 μM of MMC or of BO-1055, is presented in Supplementary Figure S2C .

    Journal: Oncotarget

    Article Title: Repairing of N-mustard derivative BO-1055 induced DNA damage requires NER, HR, and MGMT-dependent DNA repair mechanisms

    doi:

    Figure Lengend Snippet: BO-1055 induces DDR and cell death A. Immunoblots showing DDR through the detection of the phosphorylation of ATM Ser1981(ATM-S1981p), Chk1 Ser 345 (Chk1-S345p), or Chk2 Thr 68 (Chk2-T68p), following the exposure of MCF-7 cells to 5, 10, or 20 μM of BO-1055 for 6-h. Cells treated with 0.1 mM of H 2 O 2 and 10 J/m 2 of UV for 30 min served as positive controls. B. The same experiment described in (A), cells were exposed to 5 μM of MMC or of BO-1055 for 0, 1, 6, or 12 hours. C. Immunohistochemical staining for the DNA damage marker γ-H2AX (green) and nucleus DAPI (blue) of cultured MCF-7 cells was conducted following incubation with 5 μM of MMC or BO-1055 for 24-h. D. FACS histogram analysis of DNA content. PI staining in fixed cells was performed following the exposure of cultured MCF-7 cells to the indicated doses of MMC or BO-1055 for the indicated times. E. FACS dot-blot analysis for cell death. AnnexinV/PI double staining in living cells was conducted following the exposure of cultured MCF-7 cells to 5 μM of MMC or of BO-1055 for the indicated times. The experiment of (D) and (E) were performed three times, and the quantitative results expressed as the mean ± SEM are respectively presented in Supplementary Figure S2A and S2B . The cell death, assessed in cells treated with 20 μM of MMC or of BO-1055, is presented in Supplementary Figure S2C .

    Article Snippet: Cells on coverslips were then briefly rinsed with PBS and permeabilized with 0.5% Triton X-100 for 10 min, before being stained with a primary antibody against γ-H2AX (clone JBW301; Merck-Millipore).

    Techniques: Western Blot, Immunohistochemistry, Staining, Marker, Cell Culture, Incubation, FACS, Dot Blot, Double Staining

    MGMT-mediated repair is required to repair BO-1055-induced, but not melphalan-induced, lesions A. Immunoblot analysis showing endogenous MGMT expression in cells. B. DDR assessed by detecting the phosphorylation of Chk1 Ser 345 (Chk1-S345p), Chk2 Thr 68 (Chk2-T68p), or P53 Ser 15 (P53-S15p), following the exposure of HEK293T cells to 5 μM of MMC or of BO-1055 for 0, 1, 6, or 12 hours. C. DDR induced by BO-1055 in MGMT knockdown MCF-7 cells. D. Immunohistochemical staining of the DNA damage marker γ-H2AX (green) and the nucleus DAPI (blue) in MCF-7 cells cultured with siRNA knockdown of MGMT, followed treatment with or without 5 μM of BO-1055 for 24-h. E. Detection of DDR in MCF-7 cells transfected with control siRNA or siRNA knockdown of MGMT, following treatment with or without 5 μM of melphalan or 5 μM of BO-1055 for 6-h. F. Detection of DDR in HEK293T cells transfected with a control vector or an MGMT expression vector, following treatment with or without 5 μM of melphalan or 5 μM of BO-1055 for 6-h. G. In vitro clonogenic survival of MCF-7 cells with knockdown of MGMT by siRNA, in MCF-7 cells exposed to the indicated doses of melphalan for 6-h.

    Journal: Oncotarget

    Article Title: Repairing of N-mustard derivative BO-1055 induced DNA damage requires NER, HR, and MGMT-dependent DNA repair mechanisms

    doi:

    Figure Lengend Snippet: MGMT-mediated repair is required to repair BO-1055-induced, but not melphalan-induced, lesions A. Immunoblot analysis showing endogenous MGMT expression in cells. B. DDR assessed by detecting the phosphorylation of Chk1 Ser 345 (Chk1-S345p), Chk2 Thr 68 (Chk2-T68p), or P53 Ser 15 (P53-S15p), following the exposure of HEK293T cells to 5 μM of MMC or of BO-1055 for 0, 1, 6, or 12 hours. C. DDR induced by BO-1055 in MGMT knockdown MCF-7 cells. D. Immunohistochemical staining of the DNA damage marker γ-H2AX (green) and the nucleus DAPI (blue) in MCF-7 cells cultured with siRNA knockdown of MGMT, followed treatment with or without 5 μM of BO-1055 for 24-h. E. Detection of DDR in MCF-7 cells transfected with control siRNA or siRNA knockdown of MGMT, following treatment with or without 5 μM of melphalan or 5 μM of BO-1055 for 6-h. F. Detection of DDR in HEK293T cells transfected with a control vector or an MGMT expression vector, following treatment with or without 5 μM of melphalan or 5 μM of BO-1055 for 6-h. G. In vitro clonogenic survival of MCF-7 cells with knockdown of MGMT by siRNA, in MCF-7 cells exposed to the indicated doses of melphalan for 6-h.

    Article Snippet: Cells on coverslips were then briefly rinsed with PBS and permeabilized with 0.5% Triton X-100 for 10 min, before being stained with a primary antibody against γ-H2AX (clone JBW301; Merck-Millipore).

    Techniques: Expressing, Immunohistochemistry, Staining, Marker, Cell Culture, Transfection, Plasmid Preparation, In Vitro

    (A) Western blotting analysis of wild-type and S260A-mutated XRCC4 in M10-derived transformants. Proliferating cell nuclear antigen (PCNA) is shown as the loading control. (B) Radiosensitivity of M10-XRCC4 WT , M10-XRCC4 S320A and M10-CMV cells measured by colony formation assay in 0.2% w/v agarose. Each symbol represents the average surviving fraction obtained from 8–10 repeated experiments, with the error bar indicating standard deviation. The P value was calculated by applying the data to Welch’s one-sided t -test. * P = 0.049, ** P = 0.023, *** P = 0.017. (C): Number of γ-H2AX foci per cell in M10-XRCC4 WT , M10-XRCC4 S320A and M10-CMV cells. Cells were harvested 4 h after 1 Gy irradiation or without irradiation and subjected to immunostaining using anti-γ-H2AX antibody and fluorescent-labeled secondary antibody. More than 60 cells were examined for each group. Each circle represents the number of foci in a single cell, and the horizontal bar shows the average number of foci per cell in the group. The P -value was calculated by applying the data to Welch’s one-sided t -test.

    Journal: Journal of Radiation Research

    Article Title: In cellulo phosphorylation of DNA double-strand break repair protein XRCC4 on Ser260 by DNA-PK

    doi: 10.1093/jrr/rry072

    Figure Lengend Snippet: (A) Western blotting analysis of wild-type and S260A-mutated XRCC4 in M10-derived transformants. Proliferating cell nuclear antigen (PCNA) is shown as the loading control. (B) Radiosensitivity of M10-XRCC4 WT , M10-XRCC4 S320A and M10-CMV cells measured by colony formation assay in 0.2% w/v agarose. Each symbol represents the average surviving fraction obtained from 8–10 repeated experiments, with the error bar indicating standard deviation. The P value was calculated by applying the data to Welch’s one-sided t -test. * P = 0.049, ** P = 0.023, *** P = 0.017. (C): Number of γ-H2AX foci per cell in M10-XRCC4 WT , M10-XRCC4 S320A and M10-CMV cells. Cells were harvested 4 h after 1 Gy irradiation or without irradiation and subjected to immunostaining using anti-γ-H2AX antibody and fluorescent-labeled secondary antibody. More than 60 cells were examined for each group. Each circle represents the number of foci in a single cell, and the horizontal bar shows the average number of foci per cell in the group. The P -value was calculated by applying the data to Welch’s one-sided t -test.

    Article Snippet: Slides were incubated first with 5% bovine serum albumin–containing PBS(–) (BSA-PBS) for 1 h at room temperature for blocking and then with anti-γ-H2AX rabbit polyclonal antibody (Merck Millipore, 05–636) in BSA-PBS at 4°C overnight.

    Techniques: Western Blot, Derivative Assay, Colony Assay, Standard Deviation, Irradiation, Immunostaining, Labeling

    DSBs were analysed using the γ-H2AX assay ( A ) The induction of DSBs in R1 and SiHa was significantly higher after addition of PARP1- i to cDDP-based thermochermotherapy. In HeLa cells this was not found to be significant, although a trend is seen. R1: p = 0.048, SiHa: p = 0.035, HeLa: p = 0.068 From left to right: R1, SiHa, Hela cells. ( B ) One representative cell is depicted for each condition. Bars represent the mean of three independent experiments with the standard error of the mean (SEM). * p

    Journal: Oncotarget

    Article Title: Enhancing synthetic lethality of PARP-inhibitor and cisplatin in BRCA-proficient tumour cells with hyperthermia

    doi: 10.18632/oncotarget.15922

    Figure Lengend Snippet: DSBs were analysed using the γ-H2AX assay ( A ) The induction of DSBs in R1 and SiHa was significantly higher after addition of PARP1- i to cDDP-based thermochermotherapy. In HeLa cells this was not found to be significant, although a trend is seen. R1: p = 0.048, SiHa: p = 0.035, HeLa: p = 0.068 From left to right: R1, SiHa, Hela cells. ( B ) One representative cell is depicted for each condition. Bars represent the mean of three independent experiments with the standard error of the mean (SEM). * p

    Article Snippet: After centrifugation, cells were stained with antibodies against γ-H2AX-FITC (4 μg/ml, Merck Millipore, USA).

    Techniques:

    The effects of PARP1- i , HT, cDDP and combined treatments on BRCA2, Rad51 and cell survival are shown ( A ) Western blots demonstrating R1, SiHa and HeLa cells are BRCA2 proficient. After HT, BRCA2 is downregulated. ( B ) γ-H2AX and Rad51 co-localization, to investigate activity of homologous recombination. ( C ) Clonogenic assays were performed to study the effect of the different treatment combinations, 10–12 days after treatments. The addition of PARP1- i to cDDP-based thermochemotherapy resulted in a significantly lower cell survival compared to cDDP-based thermochemotherapy alone. R1: p = 0.0008, SiHa: p = 0.034, HeLa: p = 0.021. The bar graph shows the mean of at least five independent experiments. From left to right: R1, SiHa, Hela cells. * p

    Journal: Oncotarget

    Article Title: Enhancing synthetic lethality of PARP-inhibitor and cisplatin in BRCA-proficient tumour cells with hyperthermia

    doi: 10.18632/oncotarget.15922

    Figure Lengend Snippet: The effects of PARP1- i , HT, cDDP and combined treatments on BRCA2, Rad51 and cell survival are shown ( A ) Western blots demonstrating R1, SiHa and HeLa cells are BRCA2 proficient. After HT, BRCA2 is downregulated. ( B ) γ-H2AX and Rad51 co-localization, to investigate activity of homologous recombination. ( C ) Clonogenic assays were performed to study the effect of the different treatment combinations, 10–12 days after treatments. The addition of PARP1- i to cDDP-based thermochemotherapy resulted in a significantly lower cell survival compared to cDDP-based thermochemotherapy alone. R1: p = 0.0008, SiHa: p = 0.034, HeLa: p = 0.021. The bar graph shows the mean of at least five independent experiments. From left to right: R1, SiHa, Hela cells. * p

    Article Snippet: After centrifugation, cells were stained with antibodies against γ-H2AX-FITC (4 μg/ml, Merck Millipore, USA).

    Techniques: Western Blot, Activity Assay, Homologous Recombination