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    • annexin v pe apoptosis detection kit  (Vazyme Biotech Co)
    96
    Vazyme Biotech Co annexin v pe apoptosis detection kit
    BAF312 decreases S1PR1 and P-STAT3 levels and promotes <t>apoptosis</t> in SKOV3DR cells. Notes: ( A ) The chemical structure of BAF312. ( B ) MTT assay analysis of the viability of SKOV3DR cell lines after treatment with BAF312 for 72 h. ( C ) qPCR analysis of S1PR1 expression in SKOV3DR cells following treatment with 10 µM BAF312 for 2 days. Mean ± SEM, n = 3, ***P < 0.001. ( D ) Western blot analysis of the protein levels of S1PR1 and P-STAT3 in SKOV3DR cells following treatment with 10 µM BAF312 for 2 days. ( E ) MTT assay analysis of the viability of SKOV3DR-siRNA cells treated with BAF312 for 72 h. ( F ) MTT assay analysis of the viability of SKOV3DR cells treated with the indicated concentrations of DDP for 72 h. Mean ± SEM, n=5, ns P>0.05. ( G ) Apoptosis assay analysis of the apoptotic ratio of SKOV3DR cell lines after incubation with 10 µM BAF312, 10 µM DDP, and 5 µM BAF312 + 5 µM DDP for 2 days. ( H ) The statistical results of the apoptotic ratio for SKOV3DR cells. Mean ± SEM, n = 3, **P < 0.01, ***P < 0.001.
    Annexin V Pe Apoptosis Detection Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v pe apoptosis detection kit/product/Vazyme Biotech Co
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    annexin v pe apoptosis detection kit - by Bioz Stars, 2023-06
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    BAF312 decreases S1PR1 and P-STAT3 levels and promotes apoptosis in SKOV3DR cells. Notes: ( A ) The chemical structure of BAF312. ( B ) MTT assay analysis of the viability of SKOV3DR cell lines after treatment with BAF312 for 72 h. ( C ) qPCR analysis of S1PR1 expression in SKOV3DR cells following treatment with 10 µM BAF312 for 2 days. Mean ± SEM, n = 3, ***P < 0.001. ( D ) Western blot analysis of the protein levels of S1PR1 and P-STAT3 in SKOV3DR cells following treatment with 10 µM BAF312 for 2 days. ( E ) MTT assay analysis of the viability of SKOV3DR-siRNA cells treated with BAF312 for 72 h. ( F ) MTT assay analysis of the viability of SKOV3DR cells treated with the indicated concentrations of DDP for 72 h. Mean ± SEM, n=5, ns P>0.05. ( G ) Apoptosis assay analysis of the apoptotic ratio of SKOV3DR cell lines after incubation with 10 µM BAF312, 10 µM DDP, and 5 µM BAF312 + 5 µM DDP for 2 days. ( H ) The statistical results of the apoptotic ratio for SKOV3DR cells. Mean ± SEM, n = 3, **P < 0.01, ***P < 0.001.

    Journal: International Journal of Nanomedicine

    Article Title: Nanoparticle BAF312@CaP-NP Overcomes Sphingosine-1-Phosphate Receptor-1-Mediated Chemoresistance Through Inhibiting S1PR1/P-STAT3 Axis in Ovarian Carcinoma

    doi: 10.2147/IJN.S248667

    Figure Lengend Snippet: BAF312 decreases S1PR1 and P-STAT3 levels and promotes apoptosis in SKOV3DR cells. Notes: ( A ) The chemical structure of BAF312. ( B ) MTT assay analysis of the viability of SKOV3DR cell lines after treatment with BAF312 for 72 h. ( C ) qPCR analysis of S1PR1 expression in SKOV3DR cells following treatment with 10 µM BAF312 for 2 days. Mean ± SEM, n = 3, ***P < 0.001. ( D ) Western blot analysis of the protein levels of S1PR1 and P-STAT3 in SKOV3DR cells following treatment with 10 µM BAF312 for 2 days. ( E ) MTT assay analysis of the viability of SKOV3DR-siRNA cells treated with BAF312 for 72 h. ( F ) MTT assay analysis of the viability of SKOV3DR cells treated with the indicated concentrations of DDP for 72 h. Mean ± SEM, n=5, ns P>0.05. ( G ) Apoptosis assay analysis of the apoptotic ratio of SKOV3DR cell lines after incubation with 10 µM BAF312, 10 µM DDP, and 5 µM BAF312 + 5 µM DDP for 2 days. ( H ) The statistical results of the apoptotic ratio for SKOV3DR cells. Mean ± SEM, n = 3, **P < 0.01, ***P < 0.001.

    Article Snippet: Cells were seeded in 6-well plates at a density of 5×10 5 cells per well overnight and treated with the indicated concentrations of the indicated drugs for 48 h. Cells were collected and detected by an Annexin V-PE apoptosis detection kit (Vazyme, China).

    Techniques: MTT Assay, Expressing, Western Blot, Apoptosis Assay, Incubation

    BAF312@CaP-NPs dramatically boost the apoptosis of SKOV3DR cells by inhibiting S1PR1 and downregulating P-STAT3. Notes: ( A ) Fluorescence microscopy analysis of the cellular uptake of tumor-targeted shell-core nanoparticles in SKOV3DR cell lines. ( B ) Apoptosis assay analysis of the apoptotic ratio of SKOV3DR cell lines after incubation with 10 µM BAF312, 10 µM BAF312@CaP-NPs and an equal amount of control CaP-NPs for 2 days. ( C ) The statistical results of the apoptotic ratio for SKOV3DR cells. Mean ± SEM, n = 3, ns P > 0.05, *P < 0.05, ***P < 0.001. ( D ) Calcium indicator and PI staining analysis of apoptosis in SKOV3DR cells following incubation with 10 µM BAF312, 10 µM BAF312@CaP-NPs and an equal amount of control CaP-NPs for 3 days. The red represents PI positivity, the green represents calcium positivity, and the yellow represents both PI and calcium positivity. ( E ) MTT assay analysis of the viability of SKOV3DR cells treated with BAF312 for 12 h and 24 h in pH 6.0 and pH 7.4 medium, respectively. ( F ) Western blot analysis of the protein levels of S1PR1, P-STAT3, and survivin in SKOV3DR cells following treatment with 10 µM BAF312, 10 µM BAF312@CaP-NPs and an equal amount of control CaP-NPs for 2 days. ( G ) Schematic diagram of BAF312@CaP-NPs killing ovarian cancer cells.

    Journal: International Journal of Nanomedicine

    Article Title: Nanoparticle BAF312@CaP-NP Overcomes Sphingosine-1-Phosphate Receptor-1-Mediated Chemoresistance Through Inhibiting S1PR1/P-STAT3 Axis in Ovarian Carcinoma

    doi: 10.2147/IJN.S248667

    Figure Lengend Snippet: BAF312@CaP-NPs dramatically boost the apoptosis of SKOV3DR cells by inhibiting S1PR1 and downregulating P-STAT3. Notes: ( A ) Fluorescence microscopy analysis of the cellular uptake of tumor-targeted shell-core nanoparticles in SKOV3DR cell lines. ( B ) Apoptosis assay analysis of the apoptotic ratio of SKOV3DR cell lines after incubation with 10 µM BAF312, 10 µM BAF312@CaP-NPs and an equal amount of control CaP-NPs for 2 days. ( C ) The statistical results of the apoptotic ratio for SKOV3DR cells. Mean ± SEM, n = 3, ns P > 0.05, *P < 0.05, ***P < 0.001. ( D ) Calcium indicator and PI staining analysis of apoptosis in SKOV3DR cells following incubation with 10 µM BAF312, 10 µM BAF312@CaP-NPs and an equal amount of control CaP-NPs for 3 days. The red represents PI positivity, the green represents calcium positivity, and the yellow represents both PI and calcium positivity. ( E ) MTT assay analysis of the viability of SKOV3DR cells treated with BAF312 for 12 h and 24 h in pH 6.0 and pH 7.4 medium, respectively. ( F ) Western blot analysis of the protein levels of S1PR1, P-STAT3, and survivin in SKOV3DR cells following treatment with 10 µM BAF312, 10 µM BAF312@CaP-NPs and an equal amount of control CaP-NPs for 2 days. ( G ) Schematic diagram of BAF312@CaP-NPs killing ovarian cancer cells.

    Article Snippet: Cells were seeded in 6-well plates at a density of 5×10 5 cells per well overnight and treated with the indicated concentrations of the indicated drugs for 48 h. Cells were collected and detected by an Annexin V-PE apoptosis detection kit (Vazyme, China).

    Techniques: Fluorescence, Microscopy, Apoptosis Assay, Incubation, Staining, MTT Assay, Western Blot