Journal: Biomolecules

Article Title: Crinamine Induces Apoptosis and Inhibits Proliferation, Migration, and Angiogenesis in Cervical Cancer SiHa Cells

doi: 10.3390/biom9090494

Figure Lengend Snippet: Crinamine-mediated apoptosis activation in cervical cancer cells. ( a ) Annexin V and PI staining of SiHa cells treated with cisplatin, crinamine, or DMSO for 16 h. ( b ) Fluorescent intensity of Annexin V staining from ( a ); results are shown as means ± SD from triplicate measurements. ( c ) Caspase-3/7 activity of crinamine in SiHa cells non-treated or treated with the indicated concentrations of cisplatin or crinamine for 24 h. Caspase activity is normalized to the DMSO control and is reported as means ± SD from triplicate experiments. ( d ) Immunofluorescence staining of histone γ-H2AX in SiHa cells treated with the indicated concentrations of cisplatin, crinamine, or DMSO for 4 and 24 h. Panels represent phase contrast (PhL) images, DAPI nuclear staining (blue), γ-H2AX foci (red), and merged images. ( e ) Percentage of γ-H2AX foci-positive cells quantified from five fields per treatment obtained from duplicate measurements. Results are representative of data observed on two separate occasions. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: SiHa cells were seeded in 24-well plates and treated with the indicated concentration of cisplatin, crinamine, or 0.16% DMSO for 16 h. Cells were stained with Annexin V-fluorescein isothiocyanate (FITC) using the Annexin V-FITC Early Apoptosis Detection Kit (#6592; Cell Signaling Technology, Danvers, MA, USA).

Techniques: Activation Assay, Staining, Activity Assay, Immunofluorescence

Journal: Annals of Translational Medicine

Article Title: Oligoadenylate synthetases-like is a prognostic biomarker and therapeutic target in pancreatic ductal adenocarcinoma

doi: 10.21037/atm-21-6618

Figure Lengend Snippet: Knock-down of OASL inhibits cells proliferation, invasion and promotes apoptosis of PDAC cells. (A) The OASL protein expression was significantly decreased upon si-OASL transfection. (B) Cell viability was measured by MTT assay (Panc-1, si-NC vs. si-OASL-1. P<0.05. si-NC vs. si-OASL-2. P<0.05. Mia paca-2, si-NC vs. si-OASL-1. P<0.05. si-NC vs. si-OASL-2. P<0.05. Aspc-1, si-NC vs. si-OASL-1. P<0.05. si-NC vs. si-OASL-2. P<0.05). (C) The invasive ability of si-OASL groups appeared sharply reduced in the Transwell assay. Crystal violet-stained invasive cells were captured using an inverted microscope at ×100 magnification. (D) Obviously more apoptosis was observed in si-OASL groups. Experiments were repeated a minimum of 3 times. OASL, oligoadenylate synthetases-like; PDAC, pancreatic ductal adenocarcinoma; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.

Article Snippet: Flow cytometric analysis Apoptosis was detected by annexin V apoptosis kit (Vazyme, Jiangsu, China).

Techniques: Expressing, Transfection, MTT Assay, Transwell Assay, Staining, Inverted Microscopy

Journal: Molecules

Article Title: Schisandrin B Attenuates Hepatic Stellate Cell Activation and Promotes Apoptosis to Protect against Liver Fibrosis

doi: 10.3390/molecules26226882

Figure Lengend Snippet: Sch B induced HSCs apoptosis. ( A ) HSC-T6 cells were treated with 0, 25 or 50 μM Sch B for 24 h, ( B ) LX-2 cells were treated with 0, 25 or 50 μM Sch B for 24 h and apoptosis was assessed by flow cytometry. The results shown are means ± SD, *** p < 0.001 compared with the TGF-β1 group.

Article Snippet: FITC/PI cell apoptosis kit were purchased from Keygen Biotechnology Co., Ltd (Nanjing, China).

Techniques: Flow Cytometry

Journal: Molecules

Article Title: Schisandrin B Attenuates Hepatic Stellate Cell Activation and Promotes Apoptosis to Protect against Liver Fibrosis

doi: 10.3390/molecules26226882

Figure Lengend Snippet: Effects of Sch B on hepatic stellate cells apoptosis factors. The expression of Bcl-2, Bax, and Caspase-3 of HSC-T6 ( A ) and LX-2 ( B ) were determined by western blots. ( C ) Densitometric analysis of cleaved-Cas3, Bax and Bcl-2 of HSC-T6 cells. ( D ) Densitometric analysis of cleaved-Cas3, Bax and Bcl-2 of LX-2 cells. The protein density of bands in western blots were detected using Bio-Rad Quantity One software. Data are presented as means ± SD, ** p < 0.01, *** p < 0.001 compared with the TGF-β1 group.

Article Snippet: FITC/PI cell apoptosis kit were purchased from Keygen Biotechnology Co., Ltd (Nanjing, China).

Techniques: Expressing, Western Blot, Software

Journal: Cell Death & Disease

Article Title: DNA hypermethylation modification promotes the development of hepatocellular carcinoma by depressing the tumor suppressor gene ZNF334

doi: 10.1038/s41419-022-04895-6

Figure Lengend Snippet: A mRNA expression of ZNF334 in different cell lines. B mRNA expression of ZNF334 in overexpression Huh7 cell line (ZNF334-OE) and SNU387 interfering cell line(sh-ZNF334). C Protein expression of ZNF334 in different cell lines. D Protein expression of ZNF334 in overexpression Huh7 cell line (ZNF334-OE) and SNU387 interfering cell line(sh-ZNF334). E Representative images of ZNF334 expression in HepG2, SNU387 (immunofluorescence staining, ×200, blue fluorescence represents nucleus, red fluorescence represents ZNF334). F Cell proliferation curve of ZNF334-OE and ZNF334-NC by CCK8 in Huh7 cell line. G Cell proliferation curve of sh-ZNF334 and sh-NC by CCK8 in SNU387 cell line. H Proliferation ratio of ZNF334-OE and ZNF334-NC by EdU proliferation assay. I Apoptosis ratio of ZNF334-OE and ZNF334-NC by TUNEL apoptosis assay. J Representative images of proliferation of ZNF334-OE and ZNF334-NC by EdU (immunofluorescence staining, ×200, blue fluorescence represents nucleus, red fluorescence represents proliferating cells). K Cell apoptosis of ZNF334-OE and ZNF334-NC by flow cytometry. L Representative images of apoptosis of ZNF334-OE and ZNF334-NC by EdU (immunofluorescence staining, ×200, blue fluorescence represents nucleus, green fluorescence represents nucleus undergoing apoptosis). M Representative images and statistical analysis of ZNF334-OE, ZNF334-NC, sh-ZNF334, and sh-NC by plate clone formation assay. N Representative images and statistical analysis of ZNF334-OE, ZNF334-NC, sh-ZNF334, and sh-NC by sphere formation assay. ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Annexin V: FITC Cell Apoptosis Detection Kit (BD, #556547) was used to determinate the cell apoptosis.

Techniques: Expressing, Over Expression, Immunofluorescence, Staining, Fluorescence, Proliferation Assay, TUNEL Assay, Apoptosis Assay, Flow Cytometry, Tube Formation Assay

Journal: Frontiers in Cardiovascular Medicine

Article Title: Proteomics Revealed That Mitochondrial Function Contributed to the Protective Effect of Herba Siegesbeckiae Against Cardiac Ischemia/Reperfusion Injury

doi: 10.3389/fcvm.2022.895797

Figure Lengend Snippet: Effect of Herba Siegesbeckiae (HS) on myocardial histology, apoptosis, and injury after Ischemia/Reperfusion (I/R). (A) Representative HE-stained (a1–a5) and TUNEL staining (a6–a10) photographs in Sham (a1,a6), I/R (a2,a7), HS1 + I/R (a3,a8), HS2 + I/R (a4,a9), and HS4 + I/R (a5,a10) groups. Bar = 50 μm. (B) The quantitative analysis of TUNEL-positive cells/field in different groups ( n = 3). (C–G) The plasma contents of CK-MB (C) , cTnT (D) , LDH (E) , and activities of ALT (F) and AST (G) in different groups ( n = 9). * P < 0.05 vs. Sham group, # P < 0.05 vs. I/R group.

Article Snippet: Serial paraffin sections (5 μm) were prepared and stained with HE, and a TUNEL assay was performed using a FITC cell apoptosis detection kit (G1501, Servicebio, Wuhan, China), according to the manufacturer’s instruction ( ).

Techniques: Staining, TUNEL Assay

Journal: Frontiers in Cardiovascular Medicine

Article Title: Proteomics Revealed That Mitochondrial Function Contributed to the Protective Effect of Herba Siegesbeckiae Against Cardiac Ischemia/Reperfusion Injury

doi: 10.3389/fcvm.2022.895797

Figure Lengend Snippet: A diagrammatic sketch displaying the pathways that lead to the protective effect of Herba Siegesbeckiae (HS) on Ischemia/Reperfusion (I/R)-induced cardiac injury. I/R upregulates the expression of Adgb, Cbr1, Decr1, Uchl5, and Lmo7, and downregulates the expression of Bdh1, Ckmt2, and COX7A, resulting in oxidative stress and NLRP3 inflammasome activation with enhancement of inflammation, pyroptosis, apoptosis, and infarct size. Nevertheless, HS pre-treatment reverses all the above alterations. The cardioprotective role of HS relies on the inhibition of oxidative stress and NLRP3 inflammasome, and the restoration of mitochondrial functions.

Article Snippet: Serial paraffin sections (5 μm) were prepared and stained with HE, and a TUNEL assay was performed using a FITC cell apoptosis detection kit (G1501, Servicebio, Wuhan, China), according to the manufacturer’s instruction ( ).

Techniques: Expressing, Activation Assay, Inhibition