Journal: Annals of Translational Medicine
Article Title: Oligoadenylate synthetases-like is a prognostic biomarker and therapeutic target in pancreatic ductal adenocarcinoma
Figure Lengend Snippet: Knock-down of OASL inhibits cells proliferation, invasion and promotes apoptosis of PDAC cells. (A) The OASL protein expression was significantly decreased upon si-OASL transfection. (B) Cell viability was measured by MTT assay (Panc-1, si-NC vs. si-OASL-1. P<0.05. si-NC vs. si-OASL-2. P<0.05. Mia paca-2, si-NC vs. si-OASL-1. P<0.05. si-NC vs. si-OASL-2. P<0.05. Aspc-1, si-NC vs. si-OASL-1. P<0.05. si-NC vs. si-OASL-2. P<0.05). (C) The invasive ability of si-OASL groups appeared sharply reduced in the Transwell assay. Crystal violet-stained invasive cells were captured using an inverted microscope at ×100 magnification. (D) Obviously more apoptosis was observed in si-OASL groups. Experiments were repeated a minimum of 3 times. OASL, oligoadenylate synthetases-like; PDAC, pancreatic ductal adenocarcinoma; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.
Article Snippet: Flow cytometric analysis Apoptosis was detected by annexin V apoptosis kit (Vazyme, Jiangsu, China).
Techniques: Expressing, Transfection, MTT Assay, Transwell Assay, Staining, Inverted Microscopy