Journal: Oncology Letters

Article Title: Effect of microRNA-27b on cisplatin chemotherapy sensitivity of oral squamous cell carcinoma via FZD7 signaling pathway

doi: 10.3892/ol.2019.10347

Figure Lengend Snippet: Effect of miR-27b overexpression on apoptosis of drug-resistant OSCC cells. Cisplatin-resistant cell group: Drug resistance group, DRG; Cisplatin resistance + miR-27b mimic group: Drug resistance + miR-27b mimic group, DRG+miR-27b mimic. *P<0.05; compared with DRG. OSCC, oral squamous cell carcinoma.

Article Snippet: The apoptosis rate was measured according to the procedure of the apoptosis detection kit Annexin V-FITC PI (Biotime, Shanghai, China).

Techniques: Over Expression

Journal: Antioxidants

Article Title: Pharmacological Ascorbate Elicits Anti-Cancer Activities against Non-Small Cell Lung Cancer through Hydrogen-Peroxide-Induced-DNA-Damage

doi: 10.3390/antiox12091775

Figure Lengend Snippet: Pharmacological ascorbate causes apoptotic cell death in NSCLC via H 2 O 2 production. ( A , B ) Exposure to P-AscH − (1–4 mM; 1 h) induces apoptotic cell death in NSCLC in a dose-dependent fashion. ( C , D ) The inductions of apoptosis by P-AscH − (1–4 mM; 1 h) on NSCLC were prevented by catalase co-treatment. Cells were treated with P-AscH − (0–4 mM) ± catalase (200 U/mL) for 1 h. At 24 h after treatments, the modes of cell death were characterized with flow cytometric assay using annexin V/PI co-staining ( n = 3; mean ± SEM; * p < 0.05, ** p < 0.01; *** p < 0.001, **** p < 0.0001 vs. untreated control).

Article Snippet: Annexin V/propidium iodide staining kit was purchased from ImmunoTools (Friesoythe, Germany).

Techniques: Flow Cytometry, Staining