Journal: Annals of Translational Medicine

Article Title: Oligoadenylate synthetases-like is a prognostic biomarker and therapeutic target in pancreatic ductal adenocarcinoma

doi: 10.21037/atm-21-6618

Figure Lengend Snippet: Knock-down of OASL inhibits cells proliferation, invasion and promotes apoptosis of PDAC cells. (A) The OASL protein expression was significantly decreased upon si-OASL transfection. (B) Cell viability was measured by MTT assay (Panc-1, si-NC vs. si-OASL-1. P<0.05. si-NC vs. si-OASL-2. P<0.05. Mia paca-2, si-NC vs. si-OASL-1. P<0.05. si-NC vs. si-OASL-2. P<0.05. Aspc-1, si-NC vs. si-OASL-1. P<0.05. si-NC vs. si-OASL-2. P<0.05). (C) The invasive ability of si-OASL groups appeared sharply reduced in the Transwell assay. Crystal violet-stained invasive cells were captured using an inverted microscope at ×100 magnification. (D) Obviously more apoptosis was observed in si-OASL groups. Experiments were repeated a minimum of 3 times. OASL, oligoadenylate synthetases-like; PDAC, pancreatic ductal adenocarcinoma; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.

Article Snippet: Flow cytometric analysis Apoptosis was detected by annexin V apoptosis kit (Vazyme, Jiangsu, China).

Techniques: Expressing, Transfection, MTT Assay, Transwell Assay, Staining, Inverted Microscopy

Journal: International Journal of Clinical and Experimental Pathology

Article Title: miR-27b expression in diagnosis and evaluation prognosis of prostate cancer

doi:

Figure Lengend Snippet: The cell apoptosis in difference groups. ***: P<0.05, Compared with NC group.

Article Snippet: Apoptosis analysis assay FITC apoptosis detection kit (Vazyme, US) was performed to detect cell apoptosis levels.

Techniques:

Journal: Antioxidants

Article Title: Magnolia officinalis Bark Extract Prevents Enterocyte Death in a Colitis Mouse Model by Inhibiting ROS-Mediated Necroptosis

doi: 10.3390/antiox11122435

Figure Lengend Snippet: MBE efficiently blocks SZ-induced necroptosis in both InEpCs and HT29 cells. ( A ) Effects of MBE on the viability of InEpC cells treated with the indicated extract concentration for 24 h and 48 h. ( B ) InEpC cells were pretreated with the indicated concentrations of MBE for 30 min before treatment with SZ (Smac mimetic (20 µM) and z−VAD (20 µM)) for 24 h. annexin V/PI was analyzed using flow cytometry. ( C ) The values represent the sum of the percentage of cells in A (PI+ annexin V+; necroptosis). ( D ) Levels of pRIP1, RIP1, pRIP3, RIP3, pMLKL, and MLKL were analyzed using immunoblotting. Tubulin was used as a loading control. ( E ) HT29 cells were treated with the indicated concentrations of MBE for 24 h and 48 h. ( F ) HT29 cells were pretreated with the indicated concentrations of MBE for 30 min before treatment with SZ (Smac mimetic (100 nM) and z−VAD (20 µM)) for 12 h. The expression of annexin V in HT29 cells was induced by SZ and analyzed using flow cytometry. ( G ) The values represent the sum of the percentage of cells in E (PI+ annexin V+; necroptosis). ( H ) Levels of pRIP1, RIP1, pRIP3, RIP3, pMLKL, and MLKL were analyzed using western blotting. Tubulin was used as a loading control. ** p < 0.01, *** p < 0.001, and **** p < 0.0001 (data were analyzed using an ANOVA).

Article Snippet: Cell death was assessed using an Annexin V-FITC Analysis Kit (Cat# BD556547, BD, Franklin Lakes, NJ, USA).

Techniques: Concentration Assay, Flow Cytometry, Western Blot, Expressing

Journal: Antioxidants

Article Title: Magnolia officinalis Bark Extract Prevents Enterocyte Death in a Colitis Mouse Model by Inhibiting ROS-Mediated Necroptosis

doi: 10.3390/antiox11122435

Figure Lengend Snippet: MN and HK inhibit SZ-induced expression of necroptosis proteins in HT29 cells. ( A and D ) HT29 cells were pretreated with MN and HK for 30 min before the treatment with SZ (Smac mimetic (100 nM) and z-VAD (20 µM)) for 12 h. ( B ) The values represent the sum of the percentage of cells in A (PI+ annexin V+; necroptosis). ( C and F ) Protein levels of pRIP1, RIP1, pRIP3, RIP3, pMLKL, and MLKL were analyzed using western blotting. Tubulin was used as a loading control. ( E ) The values represent the sum of the percentage of cells in D (PI+ annexin V+; necroptosis). **** p < 0.0001 (data were analyzed using an ANOVA).

Article Snippet: Cell death was assessed using an Annexin V-FITC Analysis Kit (Cat# BD556547, BD, Franklin Lakes, NJ, USA).

Techniques: Expressing, Western Blot