Journal: Biomolecules

Article Title: Crinamine Induces Apoptosis and Inhibits Proliferation, Migration, and Angiogenesis in Cervical Cancer SiHa Cells

doi: 10.3390/biom9090494

Figure Lengend Snippet: Crinamine-mediated apoptosis activation in cervical cancer cells. ( a ) Annexin V and PI staining of SiHa cells treated with cisplatin, crinamine, or DMSO for 16 h. ( b ) Fluorescent intensity of Annexin V staining from ( a ); results are shown as means ± SD from triplicate measurements. ( c ) Caspase-3/7 activity of crinamine in SiHa cells non-treated or treated with the indicated concentrations of cisplatin or crinamine for 24 h. Caspase activity is normalized to the DMSO control and is reported as means ± SD from triplicate experiments. ( d ) Immunofluorescence staining of histone γ-H2AX in SiHa cells treated with the indicated concentrations of cisplatin, crinamine, or DMSO for 4 and 24 h. Panels represent phase contrast (PhL) images, DAPI nuclear staining (blue), γ-H2AX foci (red), and merged images. ( e ) Percentage of γ-H2AX foci-positive cells quantified from five fields per treatment obtained from duplicate measurements. Results are representative of data observed on two separate occasions. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: SiHa cells were seeded in 24-well plates and treated with the indicated concentration of cisplatin, crinamine, or 0.16% DMSO for 16 h. Cells were stained with Annexin V-fluorescein isothiocyanate (FITC) using the Annexin V-FITC Early Apoptosis Detection Kit (#6592; Cell Signaling Technology, Danvers, MA, USA).

Techniques: Activation Assay, Staining, Activity Assay, Immunofluorescence

Journal: Drug Delivery

Article Title: A recombinant scFv antibody-based fusion protein that targets EGFR associated with IMPDH2 downregulation and its drug conjugate show therapeutic efficacy against esophageal cancer

doi: 10.1080/10717544.2022.2063454

Figure Lengend Snippet: DNA damage, cell cycle arrest analysis, and apoptosis of EC cells. (A,B) Flow cytometry analyses of apoptosis in KYSE150 and Eca109 cells treated with Fv-LDP-D3 and Fv-LDP-D3-AE, respectively. (C) Western blot analysis of apoptosis-related proteins in KYSE150 cells treated with Fv-LDP-D3. (D) Western blot analysis of apoptosis- and DNA damage-related protein expression in KYSE150 and Eca109 cells treated with Fv-LDP-D3-AE. (E) Cell cycle arrest in KYSE150 and Eca109 cells treated with Fv-LDP-D3-AE was determined by flow cytometry. Results are reported as means ± standard deviation (SD) ( n = 3). ** p < 0.01, *** p < 0.001 versus control group.

Article Snippet: Cells were collected according to the instructions of the Annexin V FITC Apoptosis Detection Kit (Dojindo).

Techniques: Flow Cytometry, Western Blot, Expressing, Standard Deviation

Journal: Annals of Translational Medicine

Article Title: Oligoadenylate synthetases-like is a prognostic biomarker and therapeutic target in pancreatic ductal adenocarcinoma

doi: 10.21037/atm-21-6618

Figure Lengend Snippet: Knock-down of OASL inhibits cells proliferation, invasion and promotes apoptosis of PDAC cells. (A) The OASL protein expression was significantly decreased upon si-OASL transfection. (B) Cell viability was measured by MTT assay (Panc-1, si-NC vs. si-OASL-1. P<0.05. si-NC vs. si-OASL-2. P<0.05. Mia paca-2, si-NC vs. si-OASL-1. P<0.05. si-NC vs. si-OASL-2. P<0.05. Aspc-1, si-NC vs. si-OASL-1. P<0.05. si-NC vs. si-OASL-2. P<0.05). (C) The invasive ability of si-OASL groups appeared sharply reduced in the Transwell assay. Crystal violet-stained invasive cells were captured using an inverted microscope at ×100 magnification. (D) Obviously more apoptosis was observed in si-OASL groups. Experiments were repeated a minimum of 3 times. OASL, oligoadenylate synthetases-like; PDAC, pancreatic ductal adenocarcinoma; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.

Article Snippet: Flow cytometric analysis Apoptosis was detected by annexin V apoptosis kit (Vazyme, Jiangsu, China).

Techniques: Expressing, Transfection, MTT Assay, Transwell Assay, Staining, Inverted Microscopy