annexin v fitc early apoptosis detection kit Search Results


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  • 97
    Vazyme Biotech Co annexin v
    Annexin V, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems annexin v fitc apoptosis detection kit
    ORAOV1 silencing induces <t>apoptosis</t> in HeLa cells . (A) Analysis of HeLa cell apoptosis (sub-G1 cells) upon ORAOV1 silencing by flow cytometry assay using PI staining. (B) Quantitative analysis of cell apoptosis by <t>Annexin</t> <t>V</t> and PI double staining. Data were Mean ± SE for triplicate experiments, *, P < 0.05. (C) The effect of ORAOV1 silencing on the expression of P53, Bcl-2, Caspase-3, 8 and 9, and cytochrome c (mitochondrial and cytosolic). G3PDH was used as an internal control for protein equal loading, and VDAC and β-actin was used as equal mitochondria and cytosol loading control respectively. Data was representative blots of three independent experiments.
    Annexin V Fitc Apoptosis Detection Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc annexin v fitc early apoptosis detection kit
    Crinamine-mediated <t>apoptosis</t> activation in cervical cancer cells. ( a ) <t>Annexin</t> <t>V</t> and PI staining of SiHa cells treated with cisplatin, crinamine, or DMSO for 16 h. ( b ) Fluorescent intensity of Annexin V staining from ( a ); results are shown as means ± SD from triplicate measurements. ( c ) Caspase-3/7 activity of crinamine in SiHa cells non-treated or treated with the indicated concentrations of cisplatin or crinamine for 24 h. Caspase activity is normalized to the DMSO control and is reported as means ± SD from triplicate experiments. ( d ) Immunofluorescence staining of histone γ-H2AX in SiHa cells treated with the indicated concentrations of cisplatin, crinamine, or DMSO for 4 and 24 h. Panels represent phase contrast (PhL) images, DAPI nuclear staining (blue), γ-H2AX foci (red), and merged images. ( e ) Percentage of γ-H2AX foci-positive cells quantified from five fields per treatment obtained from duplicate measurements. Results are representative of data observed on two separate occasions. * p < 0.05; ** p < 0.01; *** p < 0.001.
    Annexin V Fitc Early Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc early apoptosis detection kit/product/Cell Signaling Technology Inc
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    Dojindo Labs annexin v fitc apoptosis detection kit
    Crinamine-mediated <t>apoptosis</t> activation in cervical cancer cells. ( a ) <t>Annexin</t> <t>V</t> and PI staining of SiHa cells treated with cisplatin, crinamine, or DMSO for 16 h. ( b ) Fluorescent intensity of Annexin V staining from ( a ); results are shown as means ± SD from triplicate measurements. ( c ) Caspase-3/7 activity of crinamine in SiHa cells non-treated or treated with the indicated concentrations of cisplatin or crinamine for 24 h. Caspase activity is normalized to the DMSO control and is reported as means ± SD from triplicate experiments. ( d ) Immunofluorescence staining of histone γ-H2AX in SiHa cells treated with the indicated concentrations of cisplatin, crinamine, or DMSO for 4 and 24 h. Panels represent phase contrast (PhL) images, DAPI nuclear staining (blue), γ-H2AX foci (red), and merged images. ( e ) Percentage of γ-H2AX foci-positive cells quantified from five fields per treatment obtained from duplicate measurements. Results are representative of data observed on two separate occasions. * p < 0.05; ** p < 0.01; *** p < 0.001.
    Annexin V Fitc Apoptosis Detection Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tebu-bio sa annexin v, fitc apoptosis detection kit
    Crinamine-mediated <t>apoptosis</t> activation in cervical cancer cells. ( a ) <t>Annexin</t> <t>V</t> and PI staining of SiHa cells treated with cisplatin, crinamine, or DMSO for 16 h. ( b ) Fluorescent intensity of Annexin V staining from ( a ); results are shown as means ± SD from triplicate measurements. ( c ) Caspase-3/7 activity of crinamine in SiHa cells non-treated or treated with the indicated concentrations of cisplatin or crinamine for 24 h. Caspase activity is normalized to the DMSO control and is reported as means ± SD from triplicate experiments. ( d ) Immunofluorescence staining of histone γ-H2AX in SiHa cells treated with the indicated concentrations of cisplatin, crinamine, or DMSO for 4 and 24 h. Panels represent phase contrast (PhL) images, DAPI nuclear staining (blue), γ-H2AX foci (red), and merged images. ( e ) Percentage of γ-H2AX foci-positive cells quantified from five fields per treatment obtained from duplicate measurements. Results are representative of data observed on two separate occasions. * p < 0.05; ** p < 0.01; *** p < 0.001.
    Annexin V, Fitc Apoptosis Detection Kit, supplied by tebu-bio sa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ORAOV1 silencing induces apoptosis in HeLa cells . (A) Analysis of HeLa cell apoptosis (sub-G1 cells) upon ORAOV1 silencing by flow cytometry assay using PI staining. (B) Quantitative analysis of cell apoptosis by Annexin V and PI double staining. Data were Mean ± SE for triplicate experiments, *, P < 0.05. (C) The effect of ORAOV1 silencing on the expression of P53, Bcl-2, Caspase-3, 8 and 9, and cytochrome c (mitochondrial and cytosolic). G3PDH was used as an internal control for protein equal loading, and VDAC and β-actin was used as equal mitochondria and cytosol loading control respectively. Data was representative blots of three independent experiments.

    Journal: Molecular Cancer

    Article Title: Oral cancer overexpressed 1 (ORAOV1) regulates cell cycle and apoptosis in cervical cancer HeLa cells

    doi: 10.1186/1476-4598-9-20

    Figure Lengend Snippet: ORAOV1 silencing induces apoptosis in HeLa cells . (A) Analysis of HeLa cell apoptosis (sub-G1 cells) upon ORAOV1 silencing by flow cytometry assay using PI staining. (B) Quantitative analysis of cell apoptosis by Annexin V and PI double staining. Data were Mean ± SE for triplicate experiments, *, P < 0.05. (C) The effect of ORAOV1 silencing on the expression of P53, Bcl-2, Caspase-3, 8 and 9, and cytochrome c (mitochondrial and cytosolic). G3PDH was used as an internal control for protein equal loading, and VDAC and β-actin was used as equal mitochondria and cytosol loading control respectively. Data was representative blots of three independent experiments.

    Article Snippet: Annexin-V & PI double staining was performed using Annexin-V FITC Apoptosis Detection Kit (R&D systems, Abingdon, UK) following the manufacturer's protocol.

    Techniques: Flow Cytometry, Staining, Double Staining, Expressing

    Crinamine-mediated apoptosis activation in cervical cancer cells. ( a ) Annexin V and PI staining of SiHa cells treated with cisplatin, crinamine, or DMSO for 16 h. ( b ) Fluorescent intensity of Annexin V staining from ( a ); results are shown as means ± SD from triplicate measurements. ( c ) Caspase-3/7 activity of crinamine in SiHa cells non-treated or treated with the indicated concentrations of cisplatin or crinamine for 24 h. Caspase activity is normalized to the DMSO control and is reported as means ± SD from triplicate experiments. ( d ) Immunofluorescence staining of histone γ-H2AX in SiHa cells treated with the indicated concentrations of cisplatin, crinamine, or DMSO for 4 and 24 h. Panels represent phase contrast (PhL) images, DAPI nuclear staining (blue), γ-H2AX foci (red), and merged images. ( e ) Percentage of γ-H2AX foci-positive cells quantified from five fields per treatment obtained from duplicate measurements. Results are representative of data observed on two separate occasions. * p < 0.05; ** p < 0.01; *** p < 0.001.

    Journal: Biomolecules

    Article Title: Crinamine Induces Apoptosis and Inhibits Proliferation, Migration, and Angiogenesis in Cervical Cancer SiHa Cells

    doi: 10.3390/biom9090494

    Figure Lengend Snippet: Crinamine-mediated apoptosis activation in cervical cancer cells. ( a ) Annexin V and PI staining of SiHa cells treated with cisplatin, crinamine, or DMSO for 16 h. ( b ) Fluorescent intensity of Annexin V staining from ( a ); results are shown as means ± SD from triplicate measurements. ( c ) Caspase-3/7 activity of crinamine in SiHa cells non-treated or treated with the indicated concentrations of cisplatin or crinamine for 24 h. Caspase activity is normalized to the DMSO control and is reported as means ± SD from triplicate experiments. ( d ) Immunofluorescence staining of histone γ-H2AX in SiHa cells treated with the indicated concentrations of cisplatin, crinamine, or DMSO for 4 and 24 h. Panels represent phase contrast (PhL) images, DAPI nuclear staining (blue), γ-H2AX foci (red), and merged images. ( e ) Percentage of γ-H2AX foci-positive cells quantified from five fields per treatment obtained from duplicate measurements. Results are representative of data observed on two separate occasions. * p < 0.05; ** p < 0.01; *** p < 0.001.

    Article Snippet: SiHa cells were seeded in 24-well plates and treated with the indicated concentration of cisplatin, crinamine, or 0.16% DMSO for 16 h. Cells were stained with Annexin V-fluorescein isothiocyanate (FITC) using the Annexin V-FITC Early Apoptosis Detection Kit (#6592; Cell Signaling Technology, Danvers, MA, USA).

    Techniques: Activation Assay, Staining, Activity Assay, Immunofluorescence