amv reverse transcriptase Promega Search Results


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  • 95
    Millipore amv reverse transcriptase
    Amv Reverse Transcriptase, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher amv rt
    Contamination of commercial <t>AMV</t> reverse transcriptases (RT) with MLV sequences. A. Representative real-time, generic MLV protease ( pro ) amplification plot. Blue lines, XMRV RNA standard extracted from 22Rv1 cell culture supernatants from 10 6 –10 0 copies per reaction; burgundy lines with triangles, 4/16 (25%) water only controls tested positive for MLV pro sequences using the ABI TaqMan Fast 1-step Master Mix; bright green line, RFU, relative fluorescent units. B. Representative gel image showing nested PCR detection of 208-bp MLV polymerase ( pol ) sequences in water only control reactions using <t>Finnzymes</t> RobustI AMV RT. Lanes 1–16, water only controls; lanes 17–20, XMRV RNA extracted from 22Rv1 cell culture supernatants from 10 3 , 10 2 , and 10 copies per reaction, respectively; M. molecular weight marker.
    Amv Rt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 353 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian myoblastosis virus reverse transcriptase polymerase
    Contamination of commercial <t>AMV</t> reverse transcriptases (RT) with MLV sequences. A. Representative real-time, generic MLV protease ( pro ) amplification plot. Blue lines, XMRV RNA standard extracted from 22Rv1 cell culture supernatants from 10 6 –10 0 copies per reaction; burgundy lines with triangles, 4/16 (25%) water only controls tested positive for MLV pro sequences using the ABI TaqMan Fast 1-step Master Mix; bright green line, RFU, relative fluorescent units. B. Representative gel image showing nested PCR detection of 208-bp MLV polymerase ( pol ) sequences in water only control reactions using <t>Finnzymes</t> RobustI AMV RT. Lanes 1–16, water only controls; lanes 17–20, XMRV RNA extracted from 22Rv1 cell culture supernatants from 10 3 , 10 2 , and 10 copies per reaction, respectively; M. molecular weight marker.
    Avian Myoblastosis Virus Reverse Transcriptase Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega amv reverse transcriptase
    Encapsidation of <t>pgRNA.</t> Primer extension using oligo 1948− extended with avian myeloblastosis virus <t>(AMV)</t> reverse transcriptase (RT) was used to measure the level of 5′ ends of encapsidated and reference RNAs. (A and B) Representative gels used to measure the encapsidation efficiency of total and encapsidated pgRNA, respectively. In each gel, the positions of the pgRNA and reference RNA (ref. RNA) are indicated. A sequencing ladder is provided for reference. (C) Histogram of the efficiency of encapsidation of the 5′ end of pgRNA, with standard deviations of normalized values represented by error bars. (D) Formula used to calculate encapsidation. An asterisk (*) indicates a significant difference from the WT reference ( P
    Amv Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 6834 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian myoblastosis virus reverse transcriptase primer extension system
    Encapsidation of <t>pgRNA.</t> Primer extension using oligo 1948− extended with avian myeloblastosis virus <t>(AMV)</t> reverse transcriptase (RT) was used to measure the level of 5′ ends of encapsidated and reference RNAs. (A and B) Representative gels used to measure the encapsidation efficiency of total and encapsidated pgRNA, respectively. In each gel, the positions of the pgRNA and reference RNA (ref. RNA) are indicated. A sequencing ladder is provided for reference. (C) Histogram of the efficiency of encapsidation of the 5′ end of pgRNA, with standard deviations of normalized values represented by error bars. (D) Formula used to calculate encapsidation. An asterisk (*) indicates a significant difference from the WT reference ( P
    Avian Myoblastosis Virus Reverse Transcriptase Primer Extension System, supplied by Promega, used in various techniques. Bioz Stars score: 75/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Promega avian myeloblastosis virus amv reverse transcriptase enzyme
    Encapsidation of <t>pgRNA.</t> Primer extension using oligo 1948− extended with avian myeloblastosis virus <t>(AMV)</t> reverse transcriptase (RT) was used to measure the level of 5′ ends of encapsidated and reference RNAs. (A and B) Representative gels used to measure the encapsidation efficiency of total and encapsidated pgRNA, respectively. In each gel, the positions of the pgRNA and reference RNA (ref. RNA) are indicated. A sequencing ladder is provided for reference. (C) Histogram of the efficiency of encapsidation of the 5′ end of pgRNA, with standard deviations of normalized values represented by error bars. (D) Formula used to calculate encapsidation. An asterisk (*) indicates a significant difference from the WT reference ( P
    Avian Myeloblastosis Virus Amv Reverse Transcriptase Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 81/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian myeloblastosis virus amv reverse transcriptase buffer
    Encapsidation of <t>pgRNA.</t> Primer extension using oligo 1948− extended with avian myeloblastosis virus <t>(AMV)</t> reverse transcriptase (RT) was used to measure the level of 5′ ends of encapsidated and reference RNAs. (A and B) Representative gels used to measure the encapsidation efficiency of total and encapsidated pgRNA, respectively. In each gel, the positions of the pgRNA and reference RNA (ref. RNA) are indicated. A sequencing ladder is provided for reference. (C) Histogram of the efficiency of encapsidation of the 5′ end of pgRNA, with standard deviations of normalized values represented by error bars. (D) Formula used to calculate encapsidation. An asterisk (*) indicates a significant difference from the WT reference ( P
    Avian Myeloblastosis Virus Amv Reverse Transcriptase Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 80/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian myeloblastosis virus amv reverse transcriptase kit
    Encapsidation of <t>pgRNA.</t> Primer extension using oligo 1948− extended with avian myeloblastosis virus <t>(AMV)</t> reverse transcriptase (RT) was used to measure the level of 5′ ends of encapsidated and reference RNAs. (A and B) Representative gels used to measure the encapsidation efficiency of total and encapsidated pgRNA, respectively. In each gel, the positions of the pgRNA and reference RNA (ref. RNA) are indicated. A sequencing ladder is provided for reference. (C) Histogram of the efficiency of encapsidation of the 5′ end of pgRNA, with standard deviations of normalized values represented by error bars. (D) Formula used to calculate encapsidation. An asterisk (*) indicates a significant difference from the WT reference ( P
    Avian Myeloblastosis Virus Amv Reverse Transcriptase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian avian myeloblastosis virus reverse transcriptase
    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
    Avian Avian Myeloblastosis Virus Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian myeloblastosis virus amv reverse transcriptase enzyme kit
    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
    Avian Myeloblastosis Virus Amv Reverse Transcriptase Enzyme Kit, supplied by Promega, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian myeloblastosis virus amv reverse transcriptase primer extension system
    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
    Avian Myeloblastosis Virus Amv Reverse Transcriptase Primer Extension System, supplied by Promega, used in various techniques. Bioz Stars score: 81/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian myeloblastosis virus reverse transcriptiase
    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
    Avian Myeloblastosis Virus Reverse Transcriptiase, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian myeloblastosis virus reverse trancriptase
    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
    Avian Myeloblastosis Virus Reverse Trancriptase, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian myeloblastosis virus reverse transcriptase amv rtase
    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
    Avian Myeloblastosis Virus Reverse Transcriptase Amv Rtase, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian myeloblastosis virus reverse transcriptase primer extension kit
    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
    Avian Myeloblastosis Virus Reverse Transcriptase Primer Extension Kit, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian myeloblastosis virus reverse transcriptase taq polymerase
    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
    Avian Myeloblastosis Virus Reverse Transcriptase Taq Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian myeloblastosis virus amv rt
    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
    Avian Myeloblastosis Virus Amv Rt, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian myelobastosis virus amv
    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
    Avian Myelobastosis Virus Amv, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega primer extension system avian myeloblastosis virus reverse transcriptase kit
    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
    Primer Extension System Avian Myeloblastosis Virus Reverse Transcriptase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 81/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega primer extension system avian myeloblastosis virus amv reverse transcriptase kit
    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
    Primer Extension System Avian Myeloblastosis Virus Amv Reverse Transcriptase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 77/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian myeloblastosis virus amv reserve transcriptase
    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
    Avian Myeloblastosis Virus Amv Reserve Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega amv reverse transcriptase reaction buffer
    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
    Amv Reverse Transcriptase Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega improm ii avian myeloblastosis virus reverse transcriptase kit
    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
    Improm Ii Avian Myeloblastosis Virus Reverse Transcriptase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega primer extension amv reverse transcriptase kit
    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
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    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
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    Promega avian myeblastosis virus reverse transcriptase amv rt
    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
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    Promega transcriptase avian myeloblastosis virus
    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
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    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
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    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
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    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian <t>myeloblastosis</t> virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.
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    Image Search Results


    Contamination of commercial AMV reverse transcriptases (RT) with MLV sequences. A. Representative real-time, generic MLV protease ( pro ) amplification plot. Blue lines, XMRV RNA standard extracted from 22Rv1 cell culture supernatants from 10 6 –10 0 copies per reaction; burgundy lines with triangles, 4/16 (25%) water only controls tested positive for MLV pro sequences using the ABI TaqMan Fast 1-step Master Mix; bright green line, RFU, relative fluorescent units. B. Representative gel image showing nested PCR detection of 208-bp MLV polymerase ( pol ) sequences in water only control reactions using Finnzymes RobustI AMV RT. Lanes 1–16, water only controls; lanes 17–20, XMRV RNA extracted from 22Rv1 cell culture supernatants from 10 3 , 10 2 , and 10 copies per reaction, respectively; M. molecular weight marker.

    Journal: PLoS ONE

    Article Title: Detection of Murine Leukemia Virus or Mouse DNA in Commercial RT-PCR Reagents and Human DNAs

    doi: 10.1371/journal.pone.0029050

    Figure Lengend Snippet: Contamination of commercial AMV reverse transcriptases (RT) with MLV sequences. A. Representative real-time, generic MLV protease ( pro ) amplification plot. Blue lines, XMRV RNA standard extracted from 22Rv1 cell culture supernatants from 10 6 –10 0 copies per reaction; burgundy lines with triangles, 4/16 (25%) water only controls tested positive for MLV pro sequences using the ABI TaqMan Fast 1-step Master Mix; bright green line, RFU, relative fluorescent units. B. Representative gel image showing nested PCR detection of 208-bp MLV polymerase ( pol ) sequences in water only control reactions using Finnzymes RobustI AMV RT. Lanes 1–16, water only controls; lanes 17–20, XMRV RNA extracted from 22Rv1 cell culture supernatants from 10 3 , 10 2 , and 10 copies per reaction, respectively; M. molecular weight marker.

    Article Snippet: We also replaced the AMV RT from Finnzymes with an AMV RT from Promega and repeated the testing and found no positive reactions in the water only controls (0/16, data not shown), further supporting the contamination of the Finnzymes RT.

    Techniques: Amplification, Cell Culture, Nested PCR, Molecular Weight, Marker

    Encapsidation of pgRNA. Primer extension using oligo 1948− extended with avian myeloblastosis virus (AMV) reverse transcriptase (RT) was used to measure the level of 5′ ends of encapsidated and reference RNAs. (A and B) Representative gels used to measure the encapsidation efficiency of total and encapsidated pgRNA, respectively. In each gel, the positions of the pgRNA and reference RNA (ref. RNA) are indicated. A sequencing ladder is provided for reference. (C) Histogram of the efficiency of encapsidation of the 5′ end of pgRNA, with standard deviations of normalized values represented by error bars. (D) Formula used to calculate encapsidation. An asterisk (*) indicates a significant difference from the WT reference ( P

    Journal: Journal of Virology

    Article Title: The Arginine Clusters of the Carboxy-Terminal Domain of the Core Protein of Hepatitis B Virus Make Pleiotropic Contributions to Genome Replication ▿

    doi: 10.1128/JVI.01957-10

    Figure Lengend Snippet: Encapsidation of pgRNA. Primer extension using oligo 1948− extended with avian myeloblastosis virus (AMV) reverse transcriptase (RT) was used to measure the level of 5′ ends of encapsidated and reference RNAs. (A and B) Representative gels used to measure the encapsidation efficiency of total and encapsidated pgRNA, respectively. In each gel, the positions of the pgRNA and reference RNA (ref. RNA) are indicated. A sequencing ladder is provided for reference. (C) Histogram of the efficiency of encapsidation of the 5′ end of pgRNA, with standard deviations of normalized values represented by error bars. (D) Formula used to calculate encapsidation. An asterisk (*) indicates a significant difference from the WT reference ( P

    Article Snippet: For simultaneous detection of RNA and DNA by primer extension (as shown in Fig. and ) a cDNA of pgRNA was generated using AMV reverse transcriptase (Promega) and an unlabeled primer.

    Techniques: Sequencing

    FcRn expression in normal adult human small intestine and human intestinal epithelial cell lines. Western blots of total cellular protein (13 μg protein per lane, a ; 10 μg protein per lane, b ) isolated from the indicated source using affinity-purified rabbit antisera raised against amino acids 112–125 ( a ) or amino acids 174–188 ( b ). ( c ) RT-PCR detection of FcγRI transcripts. Total RNA (2 μg) from T84 (lanes 3 and 4), MOLT-4 (lanes 5 and 6; negative control), and U937 (lanes 1 and 2; positive control) cell lines was incubated with an oligo-dT primer with (odd-numbered lanes) or without (even-numbered lanes) avian myeloblastosis virus–RT (AMV-RT), and a nested PCR was performed with primers specific for FcγRI cDNA (top) or for β-actin (bottom).

    Journal: Journal of Clinical Investigation

    Article Title: Bidirectional FcRn-dependent IgG transport in a polarized human intestinal epithelial cell line

    doi:

    Figure Lengend Snippet: FcRn expression in normal adult human small intestine and human intestinal epithelial cell lines. Western blots of total cellular protein (13 μg protein per lane, a ; 10 μg protein per lane, b ) isolated from the indicated source using affinity-purified rabbit antisera raised against amino acids 112–125 ( a ) or amino acids 174–188 ( b ). ( c ) RT-PCR detection of FcγRI transcripts. Total RNA (2 μg) from T84 (lanes 3 and 4), MOLT-4 (lanes 5 and 6; negative control), and U937 (lanes 1 and 2; positive control) cell lines was incubated with an oligo-dT primer with (odd-numbered lanes) or without (even-numbered lanes) avian myeloblastosis virus–RT (AMV-RT), and a nested PCR was performed with primers specific for FcγRI cDNA (top) or for β-actin (bottom).

    Article Snippet: RNA (2 μg) was reverse-transcribed to cDNA with an oligo-dT primer (Promega Corp., Madison, Wisconsin, USA) and avian myeloblastosis virus reverse transcriptase (Promega Corp.).

    Techniques: Expressing, Western Blot, Isolation, Affinity Purification, Reverse Transcription Polymerase Chain Reaction, Negative Control, Positive Control, Incubation, Nested PCR

    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian myeloblastosis virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.

    Journal: Applied and Environmental Microbiology

    Article Title: Cloning and Nucleotide Sequencing of a Staphylococcus aureus Gene Encoding a Branched-Chain-Amino-Acid Transporter

    doi:

    Figure Lengend Snippet: Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian myeloblastosis virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.

    Article Snippet: To define the transcription unit more precisely, the 5′ end of the transcript was mapped with the avian myeloblastosis virus reverse transcriptase system (Promega).

    Techniques: Northern Blot, Sequencing