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  • 99
    New England Biolabs avian myeloblastosis virus amv reverse transcriptase
    Avian Myeloblastosis Virus Amv Reverse Transcriptase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore avian myeloblastosis virus amv reverse transcriptase
    Avian Myeloblastosis Virus Amv Reverse Transcriptase, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Seikagaku avian myoblastosis virus amv reverse transcriptase
    Avian Myoblastosis Virus Amv Reverse Transcriptase, supplied by Seikagaku, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian myoblastosis virus amv reverse transcriptase
    Encapsidation of <t>pgRNA.</t> Primer extension using oligo 1948− extended with avian myeloblastosis virus <t>(AMV)</t> reverse transcriptase (RT) was used to measure the level of 5′ ends of encapsidated and reference RNAs. (A and B) Representative gels used to measure the encapsidation efficiency of total and encapsidated pgRNA, respectively. In each gel, the positions of the pgRNA and reference RNA (ref. RNA) are indicated. A sequencing ladder is provided for reference. (C) Histogram of the efficiency of encapsidation of the 5′ end of pgRNA, with standard deviations of normalized values represented by error bars. (D) Formula used to calculate encapsidation. An asterisk (*) indicates a significant difference from the WT reference ( P
    Avian Myoblastosis Virus Amv Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 438 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    TaKaRa avian myelobastosis virus amv reverse transcriptase
    (A) SeHex and (B) SeSP1 transcript analysis by RT-PCR amplification after dsRNA injection . Three larval states (before death, still living and less vital) were randomly selected at each time point after injection. Total RNA was extracted and reversed to <t>cDNA</t> using <t>AMV</t> reverse transcriptase (Takara). SeHex and SeSP1 specific probes were radiolabeled with [α- 32 P]-dCTP. The specific primers SeHex-FP and SeHex-RP or SeSP1-FP and SeSP1-RP were used to amplify cDNAs in the same PCR reactions. The PCR products were separated on 2% agarose gel and transferred to a Hybond-N + nylon membrane. Hybridization, washing and signal detection of the blots were carried out as described previously. (A) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1), 96 h (P2) and120 h (P3) indicate the times (and developmental stage) after injection. (B) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1) and 96 h (P2) indicate the times (and developmental stage) after injection. The housekeeping gene β-actin was used as a reference.
    Avian Myelobastosis Virus Amv Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Roche avian myeloblastosis virus amv reverse transcriptase
    (A) SeHex and (B) SeSP1 transcript analysis by RT-PCR amplification after dsRNA injection . Three larval states (before death, still living and less vital) were randomly selected at each time point after injection. Total RNA was extracted and reversed to <t>cDNA</t> using <t>AMV</t> reverse transcriptase (Takara). SeHex and SeSP1 specific probes were radiolabeled with [α- 32 P]-dCTP. The specific primers SeHex-FP and SeHex-RP or SeSP1-FP and SeSP1-RP were used to amplify cDNAs in the same PCR reactions. The PCR products were separated on 2% agarose gel and transferred to a Hybond-N + nylon membrane. Hybridization, washing and signal detection of the blots were carried out as described previously. (A) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1), 96 h (P2) and120 h (P3) indicate the times (and developmental stage) after injection. (B) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1) and 96 h (P2) indicate the times (and developmental stage) after injection. The housekeeping gene β-actin was used as a reference.
    Avian Myeloblastosis Virus Amv Reverse Transcriptase, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa avian myeloblastosis virus amv reverse transcriptase
    (A) SeHex and (B) SeSP1 transcript analysis by RT-PCR amplification after dsRNA injection . Three larval states (before death, still living and less vital) were randomly selected at each time point after injection. Total RNA was extracted and reversed to <t>cDNA</t> using <t>AMV</t> reverse transcriptase (Takara). SeHex and SeSP1 specific probes were radiolabeled with [α- 32 P]-dCTP. The specific primers SeHex-FP and SeHex-RP or SeSP1-FP and SeSP1-RP were used to amplify cDNAs in the same PCR reactions. The PCR products were separated on 2% agarose gel and transferred to a Hybond-N + nylon membrane. Hybridization, washing and signal detection of the blots were carried out as described previously. (A) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1), 96 h (P2) and120 h (P3) indicate the times (and developmental stage) after injection. (B) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1) and 96 h (P2) indicate the times (and developmental stage) after injection. The housekeeping gene β-actin was used as a reference.
    Avian Myeloblastosis Virus Amv Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 507 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Toyobo avian myeloblastosis virus amv reverse transcriptase
    (A) SeHex and (B) SeSP1 transcript analysis by RT-PCR amplification after dsRNA injection . Three larval states (before death, still living and less vital) were randomly selected at each time point after injection. Total RNA was extracted and reversed to <t>cDNA</t> using <t>AMV</t> reverse transcriptase (Takara). SeHex and SeSP1 specific probes were radiolabeled with [α- 32 P]-dCTP. The specific primers SeHex-FP and SeHex-RP or SeSP1-FP and SeSP1-RP were used to amplify cDNAs in the same PCR reactions. The PCR products were separated on 2% agarose gel and transferred to a Hybond-N + nylon membrane. Hybridization, washing and signal detection of the blots were carried out as described previously. (A) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1), 96 h (P2) and120 h (P3) indicate the times (and developmental stage) after injection. (B) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1) and 96 h (P2) indicate the times (and developmental stage) after injection. The housekeeping gene β-actin was used as a reference.
    Avian Myeloblastosis Virus Amv Reverse Transcriptase, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare avian myeloblastosis virus amv reverse transcriptase
    (A) SeHex and (B) SeSP1 transcript analysis by RT-PCR amplification after dsRNA injection . Three larval states (before death, still living and less vital) were randomly selected at each time point after injection. Total RNA was extracted and reversed to <t>cDNA</t> using <t>AMV</t> reverse transcriptase (Takara). SeHex and SeSP1 specific probes were radiolabeled with [α- 32 P]-dCTP. The specific primers SeHex-FP and SeHex-RP or SeSP1-FP and SeSP1-RP were used to amplify cDNAs in the same PCR reactions. The PCR products were separated on 2% agarose gel and transferred to a Hybond-N + nylon membrane. Hybridization, washing and signal detection of the blots were carried out as described previously. (A) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1), 96 h (P2) and120 h (P3) indicate the times (and developmental stage) after injection. (B) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1) and 96 h (P2) indicate the times (and developmental stage) after injection. The housekeeping gene β-actin was used as a reference.
    Avian Myeloblastosis Virus Amv Reverse Transcriptase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Life Sciences Advanced Technologies avian myeloblastosis virus amv reverse transcriptase
    (A) SeHex and (B) SeSP1 transcript analysis by RT-PCR amplification after dsRNA injection . Three larval states (before death, still living and less vital) were randomly selected at each time point after injection. Total RNA was extracted and reversed to <t>cDNA</t> using <t>AMV</t> reverse transcriptase (Takara). SeHex and SeSP1 specific probes were radiolabeled with [α- 32 P]-dCTP. The specific primers SeHex-FP and SeHex-RP or SeSP1-FP and SeSP1-RP were used to amplify cDNAs in the same PCR reactions. The PCR products were separated on 2% agarose gel and transferred to a Hybond-N + nylon membrane. Hybridization, washing and signal detection of the blots were carried out as described previously. (A) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1), 96 h (P2) and120 h (P3) indicate the times (and developmental stage) after injection. (B) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1) and 96 h (P2) indicate the times (and developmental stage) after injection. The housekeeping gene β-actin was used as a reference.
    Avian Myeloblastosis Virus Amv Reverse Transcriptase, supplied by Life Sciences Advanced Technologies, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Boehringer Mannheim avian myeloblastosis virus amv reverse transcriptase
    (A) SeHex and (B) SeSP1 transcript analysis by RT-PCR amplification after dsRNA injection . Three larval states (before death, still living and less vital) were randomly selected at each time point after injection. Total RNA was extracted and reversed to <t>cDNA</t> using <t>AMV</t> reverse transcriptase (Takara). SeHex and SeSP1 specific probes were radiolabeled with [α- 32 P]-dCTP. The specific primers SeHex-FP and SeHex-RP or SeSP1-FP and SeSP1-RP were used to amplify cDNAs in the same PCR reactions. The PCR products were separated on 2% agarose gel and transferred to a Hybond-N + nylon membrane. Hybridization, washing and signal detection of the blots were carried out as described previously. (A) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1), 96 h (P2) and120 h (P3) indicate the times (and developmental stage) after injection. (B) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1) and 96 h (P2) indicate the times (and developmental stage) after injection. The housekeeping gene β-actin was used as a reference.
    Avian Myeloblastosis Virus Amv Reverse Transcriptase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Valiant avian myeloblastosis virus amv reverse transcriptase
    (A) SeHex and (B) SeSP1 transcript analysis by RT-PCR amplification after dsRNA injection . Three larval states (before death, still living and less vital) were randomly selected at each time point after injection. Total RNA was extracted and reversed to <t>cDNA</t> using <t>AMV</t> reverse transcriptase (Takara). SeHex and SeSP1 specific probes were radiolabeled with [α- 32 P]-dCTP. The specific primers SeHex-FP and SeHex-RP or SeSP1-FP and SeSP1-RP were used to amplify cDNAs in the same PCR reactions. The PCR products were separated on 2% agarose gel and transferred to a Hybond-N + nylon membrane. Hybridization, washing and signal detection of the blots were carried out as described previously. (A) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1), 96 h (P2) and120 h (P3) indicate the times (and developmental stage) after injection. (B) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1) and 96 h (P2) indicate the times (and developmental stage) after injection. The housekeeping gene β-actin was used as a reference.
    Avian Myeloblastosis Virus Amv Reverse Transcriptase, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Stratagene avian myeloblastosis virus amv reverse transcriptase
    (A) SeHex and (B) SeSP1 transcript analysis by RT-PCR amplification after dsRNA injection . Three larval states (before death, still living and less vital) were randomly selected at each time point after injection. Total RNA was extracted and reversed to <t>cDNA</t> using <t>AMV</t> reverse transcriptase (Takara). SeHex and SeSP1 specific probes were radiolabeled with [α- 32 P]-dCTP. The specific primers SeHex-FP and SeHex-RP or SeSP1-FP and SeSP1-RP were used to amplify cDNAs in the same PCR reactions. The PCR products were separated on 2% agarose gel and transferred to a Hybond-N + nylon membrane. Hybridization, washing and signal detection of the blots were carried out as described previously. (A) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1), 96 h (P2) and120 h (P3) indicate the times (and developmental stage) after injection. (B) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1) and 96 h (P2) indicate the times (and developmental stage) after injection. The housekeeping gene β-actin was used as a reference.
    Avian Myeloblastosis Virus Amv Reverse Transcriptase, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Encapsidation of pgRNA. Primer extension using oligo 1948− extended with avian myeloblastosis virus (AMV) reverse transcriptase (RT) was used to measure the level of 5′ ends of encapsidated and reference RNAs. (A and B) Representative gels used to measure the encapsidation efficiency of total and encapsidated pgRNA, respectively. In each gel, the positions of the pgRNA and reference RNA (ref. RNA) are indicated. A sequencing ladder is provided for reference. (C) Histogram of the efficiency of encapsidation of the 5′ end of pgRNA, with standard deviations of normalized values represented by error bars. (D) Formula used to calculate encapsidation. An asterisk (*) indicates a significant difference from the WT reference ( P

    Journal: Journal of Virology

    Article Title: The Arginine Clusters of the Carboxy-Terminal Domain of the Core Protein of Hepatitis B Virus Make Pleiotropic Contributions to Genome Replication ▿

    doi: 10.1128/JVI.01957-10

    Figure Lengend Snippet: Encapsidation of pgRNA. Primer extension using oligo 1948− extended with avian myeloblastosis virus (AMV) reverse transcriptase (RT) was used to measure the level of 5′ ends of encapsidated and reference RNAs. (A and B) Representative gels used to measure the encapsidation efficiency of total and encapsidated pgRNA, respectively. In each gel, the positions of the pgRNA and reference RNA (ref. RNA) are indicated. A sequencing ladder is provided for reference. (C) Histogram of the efficiency of encapsidation of the 5′ end of pgRNA, with standard deviations of normalized values represented by error bars. (D) Formula used to calculate encapsidation. An asterisk (*) indicates a significant difference from the WT reference ( P

    Article Snippet: For simultaneous detection of RNA and DNA by primer extension (as shown in Fig. and ) a cDNA of pgRNA was generated using AMV reverse transcriptase (Promega) and an unlabeled primer.

    Techniques: Sequencing

    (A) SeHex and (B) SeSP1 transcript analysis by RT-PCR amplification after dsRNA injection . Three larval states (before death, still living and less vital) were randomly selected at each time point after injection. Total RNA was extracted and reversed to cDNA using AMV reverse transcriptase (Takara). SeHex and SeSP1 specific probes were radiolabeled with [α- 32 P]-dCTP. The specific primers SeHex-FP and SeHex-RP or SeSP1-FP and SeSP1-RP were used to amplify cDNAs in the same PCR reactions. The PCR products were separated on 2% agarose gel and transferred to a Hybond-N + nylon membrane. Hybridization, washing and signal detection of the blots were carried out as described previously. (A) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1), 96 h (P2) and120 h (P3) indicate the times (and developmental stage) after injection. (B) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1) and 96 h (P2) indicate the times (and developmental stage) after injection. The housekeeping gene β-actin was used as a reference.

    Journal: BMC Molecular Biology

    Article Title: Two storage hexamerins from the beet armyworm Spodoptera exigua: Cloning, characterization and the effect of gene silencing on survival

    doi: 10.1186/1471-2199-11-65

    Figure Lengend Snippet: (A) SeHex and (B) SeSP1 transcript analysis by RT-PCR amplification after dsRNA injection . Three larval states (before death, still living and less vital) were randomly selected at each time point after injection. Total RNA was extracted and reversed to cDNA using AMV reverse transcriptase (Takara). SeHex and SeSP1 specific probes were radiolabeled with [α- 32 P]-dCTP. The specific primers SeHex-FP and SeHex-RP or SeSP1-FP and SeSP1-RP were used to amplify cDNAs in the same PCR reactions. The PCR products were separated on 2% agarose gel and transferred to a Hybond-N + nylon membrane. Hybridization, washing and signal detection of the blots were carried out as described previously. (A) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1), 96 h (P2) and120 h (P3) indicate the times (and developmental stage) after injection. (B) Lanes marked 12 h (5L2), 24 h (5L3), 36 h (5L3), 48 h (W), 72 h (P1) and 96 h (P2) indicate the times (and developmental stage) after injection. The housekeeping gene β-actin was used as a reference.

    Article Snippet: Total RNA was extracted from individual larvae using the acid guanidinium thiocyanate-phenol-chloroform method [ , ] and converted to cDNA using AMV reverse transcriptase (Takara).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Injection, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Hybridization