amplitaq gold dna polymerase Thermo Fisher Search Results


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  • 97
    Thermo Fisher amplitaq 360 dna polymerase
    Inhibition and the effect of hot-start. ( a ) Inhibition profiles of 2D9 versus <t>AmpliTaq</t> <t>Gold</t> 360 (ATaqG) in PCR. The effect of various inhibitors (SP61: bone dust, Tar, Soil, 1736; cave sediment) on polymerase activity of 2D9 and ATaqG as determined by a PCR assay in the presence of decreasing amounts of inhibitor and plotted as the average relative resistance ( n = 3, standard error) (as in Figure 4 ) of 2D9 and ATaqG with respect to ATaqG as a reference (resistance = 1). ( b ) The effect of manual hot-start on resistance of 2D9 polymerase to inhibitors tar and soil at minimal limiting amounts of inhibitor. Hot-start increases inhibitor resistance by at least a factor of two.
    Amplitaq 360 Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher amplitaq dna polymerase
    Inhibition and the effect of hot-start. ( a ) Inhibition profiles of 2D9 versus <t>AmpliTaq</t> <t>Gold</t> 360 (ATaqG) in PCR. The effect of various inhibitors (SP61: bone dust, Tar, Soil, 1736; cave sediment) on polymerase activity of 2D9 and ATaqG as determined by a PCR assay in the presence of decreasing amounts of inhibitor and plotted as the average relative resistance ( n = 3, standard error) (as in Figure 4 ) of 2D9 and ATaqG with respect to ATaqG as a reference (resistance = 1). ( b ) The effect of manual hot-start on resistance of 2D9 polymerase to inhibitors tar and soil at minimal limiting amounts of inhibitor. Hot-start increases inhibitor resistance by at least a factor of two.
    Amplitaq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 4337 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher amplitaq gold360
    Hot Start <t>DNA</t> polymerase evaluation in triplex one-step RT-PCR amplification of ABCA5, ABCA6 and ABCA7 targets . Reactions contained 0.82 μg of human trachea total RNA and an unmodified oligo(dT) 18 RT primer. The PCR primers were either unmodified, or contained CleanAmp™ Precision modifications. These reactions contained one of the following DNA polymerases: Taq , Platinum ® Taq , or <t>AmpliTaq</t> Gold ® and one of the following reverse transcriptases: M-MLV or SSIII. Reactions with M-MLV were incubated at 42°C, while reactions with SSIII were incubated at 55°C.
    Amplitaq Gold360, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1644 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher high fidelity dna polymerase
    Hot Start <t>DNA</t> polymerase evaluation in triplex one-step RT-PCR amplification of ABCA5, ABCA6 and ABCA7 targets . Reactions contained 0.82 μg of human trachea total RNA and an unmodified oligo(dT) 18 RT primer. The PCR primers were either unmodified, or contained CleanAmp™ Precision modifications. These reactions contained one of the following DNA polymerases: Taq , Platinum ® Taq , or <t>AmpliTaq</t> Gold ® and one of the following reverse transcriptases: M-MLV or SSIII. Reactions with M-MLV were incubated at 42°C, while reactions with SSIII were incubated at 55°C.
    High Fidelity Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 827 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher amplitaq dna polymerase gold
    Hot Start <t>DNA</t> polymerase evaluation in triplex one-step RT-PCR amplification of ABCA5, ABCA6 and ABCA7 targets . Reactions contained 0.82 μg of human trachea total RNA and an unmodified oligo(dT) 18 RT primer. The PCR primers were either unmodified, or contained CleanAmp™ Precision modifications. These reactions contained one of the following DNA polymerases: Taq , Platinum ® Taq , or <t>AmpliTaq</t> Gold ® and one of the following reverse transcriptases: M-MLV or SSIII. Reactions with M-MLV were incubated at 42°C, while reactions with SSIII were incubated at 55°C.
    Amplitaq Dna Polymerase Gold, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher amplitaq gold 360 polymerase
    Hot Start <t>DNA</t> polymerase evaluation in triplex one-step RT-PCR amplification of ABCA5, ABCA6 and ABCA7 targets . Reactions contained 0.82 μg of human trachea total RNA and an unmodified oligo(dT) 18 RT primer. The PCR primers were either unmodified, or contained CleanAmp™ Precision modifications. These reactions contained one of the following DNA polymerases: Taq , Platinum ® Taq , or <t>AmpliTaq</t> Gold ® and one of the following reverse transcriptases: M-MLV or SSIII. Reactions with M-MLV were incubated at 42°C, while reactions with SSIII were incubated at 55°C.
    Amplitaq Gold 360 Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher dna polymerase
    Hot Start <t>DNA</t> polymerase evaluation in triplex one-step RT-PCR amplification of ABCA5, ABCA6 and ABCA7 targets . Reactions contained 0.82 μg of human trachea total RNA and an unmodified oligo(dT) 18 RT primer. The PCR primers were either unmodified, or contained CleanAmp™ Precision modifications. These reactions contained one of the following DNA polymerases: Taq , Platinum ® Taq , or <t>AmpliTaq</t> Gold ® and one of the following reverse transcriptases: M-MLV or SSIII. Reactions with M-MLV were incubated at 42°C, while reactions with SSIII were incubated at 55°C.
    Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3457 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Inhibition and the effect of hot-start. ( a ) Inhibition profiles of 2D9 versus AmpliTaq Gold 360 (ATaqG) in PCR. The effect of various inhibitors (SP61: bone dust, Tar, Soil, 1736; cave sediment) on polymerase activity of 2D9 and ATaqG as determined by a PCR assay in the presence of decreasing amounts of inhibitor and plotted as the average relative resistance ( n = 3, standard error) (as in Figure 4 ) of 2D9 and ATaqG with respect to ATaqG as a reference (resistance = 1). ( b ) The effect of manual hot-start on resistance of 2D9 polymerase to inhibitors tar and soil at minimal limiting amounts of inhibitor. Hot-start increases inhibitor resistance by at least a factor of two.

    Journal: Nucleic Acids Research

    Article Title: Molecular breeding of polymerases for resistance to environmental inhibitors

    doi: 10.1093/nar/gkq1360

    Figure Lengend Snippet: Inhibition and the effect of hot-start. ( a ) Inhibition profiles of 2D9 versus AmpliTaq Gold 360 (ATaqG) in PCR. The effect of various inhibitors (SP61: bone dust, Tar, Soil, 1736; cave sediment) on polymerase activity of 2D9 and ATaqG as determined by a PCR assay in the presence of decreasing amounts of inhibitor and plotted as the average relative resistance ( n = 3, standard error) (as in Figure 4 ) of 2D9 and ATaqG with respect to ATaqG as a reference (resistance = 1). ( b ) The effect of manual hot-start on resistance of 2D9 polymerase to inhibitors tar and soil at minimal limiting amounts of inhibitor. Hot-start increases inhibitor resistance by at least a factor of two.

    Article Snippet: Inhibition profiles for AmpliTaq Gold 360 DNA polymerase (Applied Biosystems) were determined using an identical PCR set-up but using AmpliTaq Gold buffer and thermocycling conditions according to manufacturer’s recommendations.

    Techniques: Inhibition, Polymerase Chain Reaction, Activity Assay

    Hot Start DNA polymerase evaluation in triplex one-step RT-PCR amplification of ABCA5, ABCA6 and ABCA7 targets . Reactions contained 0.82 μg of human trachea total RNA and an unmodified oligo(dT) 18 RT primer. The PCR primers were either unmodified, or contained CleanAmp™ Precision modifications. These reactions contained one of the following DNA polymerases: Taq , Platinum ® Taq , or AmpliTaq Gold ® and one of the following reverse transcriptases: M-MLV or SSIII. Reactions with M-MLV were incubated at 42°C, while reactions with SSIII were incubated at 55°C.

    Journal: BMC Molecular Biology

    Article Title: Selective control of primer usage in multiplex one-step reverse transcription PCR

    doi: 10.1186/1471-2199-10-113

    Figure Lengend Snippet: Hot Start DNA polymerase evaluation in triplex one-step RT-PCR amplification of ABCA5, ABCA6 and ABCA7 targets . Reactions contained 0.82 μg of human trachea total RNA and an unmodified oligo(dT) 18 RT primer. The PCR primers were either unmodified, or contained CleanAmp™ Precision modifications. These reactions contained one of the following DNA polymerases: Taq , Platinum ® Taq , or AmpliTaq Gold ® and one of the following reverse transcriptases: M-MLV or SSIII. Reactions with M-MLV were incubated at 42°C, while reactions with SSIII were incubated at 55°C.

    Article Snippet: Reaction conditions included 1× PCR buffer (20 mM Tris (pH 8.4), 50 mM KCl) (Invitrogen), 1.5 mM MgCl2 (Invitrogen), gene-specific PCR primers (0.5 μM) (TriLink), oligo(dT)18 primer (1 μM) (TriLink), 0.16 mM dNTPs (New England Biolabs), 0.5 μL of human trachea total RNA or other human total RNA (Stratagene or Ambion, each at ~1.6 μg/μL), 5 U RNase Inhibitor (New England Biolabs), 50 U Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Invitrogen) or SuperScript® III Reverse Transcriptase (SSIII RT) (Invitrogen), and 0.6 U of Taq DNA Polymerase, recombinant (Invitrogen) or Platinum® Taq DNA Polymerase (Invitrogen) or AmpliTaq Gold® DNA Polymerase (Applied Biosystems), in a 50 μL reaction volume.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Polymerase Chain Reaction, Incubation